Table of Contents
Advertisement
1: Introduction
Features
Features of the CUV-TLC-50F include the following:
•
Magnetic stirring (in the CUV-TLC-50F). A stir bar is included.
•
Dry gas purge: Limits condensation and excludes oxygen from around the cuvette. Tubing for
water and gas connections is included.
•
Optical slits: Control the illuminated volume. Several removable optical slits are included.
An optional PC-based CUV-TLC-ADP adapter package comes with Windows-compatible software that
allows you to remotely start a test sequence, operate the controller box and monitor experiments. Without
the CUV-TLC-ADP, you can control the cuvette holder's temperature mechanically from the controller
box.
One side of the holder is open for visual inspection, or may be blocked with a blank slit. The other three
sides can be left open or fitted with a removable lens system. Both collimating and focusing lens systems
are available in either AR-coated BK7 glass or AR-coated fused silica and can be used in a variety of
configurations. Typically two collimating systems are used on opposite sides for absorbance
measurements, or two imaging lens assemblies are used at right angles for fluorescence measurements.
SMA 905 optical fiber connectors are provided with the lens systems.
System Setup
►
Procedure
1. Firmly fasten the CUV-TLC-50F to your table using the holes in the base of the instrument.
2. Connect a water source to either one of the two hose barbs labeled "water" on the rear side of
the CUV-TLC-50F. Use a length of tubing with 1/8" (3mm) inside diameter such as the 3603
Tygon provided. Cooling water is required for the functioning of the thermoelectric cooler.
Tap water may be used. However, a more convenient source would be a circulating bath or a
small submersible pump in a container of water.
3. Connect tubing from the other hose barb to a drain or back into the source of circulating
water. Do not exceed an input water pressure of 25 psi (1.7 bar), as damage may occur inside
the TLC50.
4. Set and maintain a cooling water flow rate of about 200 – 300 ml per minute. This flow
should require a pressure of approximately 3 – 5 psi (0.2 – 0.3 bar). If a circulating bath is
used as the water source, it may not be possible to maintain this flow rate. However, a slower
rate will likely be adequate for low temperature work, if precooled circulating water is used.
5. If using dry gas, connect a source of dry gas (typically nitrogen or air that has passed through
a desiccant) using a length of tubing with 1/8" (3mm) inside diameter, to the hose barb
labeled "gas" on the side of the CUV-TLC-50F. A flow of gas must be used to prevent
condensation on the faces of the cuvette when working below the dew point temperature. Set
the dry gas flow rate to 50 – 200 cc/min.
6. Connect the Temperature Controller to a power source and to the CUV-TLC-50F using the
electrical cords provided.
2
000-30000-000-02-0205
Advertisement
Table of Contents
