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Workflow Overview
Daily Startup Procedure for FACSAria™ Sorters
Fluidics
Turn ON
Hardware
Stream
Software
Startup
1. Turning On Hardware/Software
1
Open Air valve for Aria-2/4. Turn yellow lever on wall until it's parallel with the pipe.
1
This is not required for Aria-7
1
Aria-2/4: Turn on Aerosol Management System
2
Aria-7: Turn on Class 1 BSC by turning Fans on
and set AMS fan to 20%
2
The AMS should be operating at 20% at all times
under normal conditions. If clog occurs
Sorter Clog SOP for further instructions.
Startup, QC & Shutdown Procedure
Cleaning
&
Sort Chamber
.
FACSAria™
Optics
QC
Cell
See
Stream/Sort
QC
Accudrop
Shutdown
1
AMS On/Off
1
switch
100% evacuation
2
button
Ver.
2019-12-22
Post Sort
Cleanup/
2
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Related Manuals for BD FACSAria
Summary of Contents for BD FACSAria
- Page 1 FACSAria™ Startup, QC & Shutdown Procedure Ver. 2019-12-22 Workflow Overview Daily Startup Procedure for FACSAria™ Sorters Fluidics Turn ON Stream/Sort Post Sort Optics Cleaning & Hardware Cleanup/ Sort Chamber Stream Software Accudrop Shutdown Startup 1. Turning On Hardware/Software Open Air valve for Aria-2/4. Turn yellow lever on wall until it’s parallel with the pipe.
- Page 2 Turn ON FACSAria™ sorter. Once computer is fully on - press the two buttons on the left side Laser Switch of the FACSAria™. Make sure to turn ON the Main Power first and then the Laser Switch button. Launch OBIS/Coherent Connection Software ...
- Page 3 2. Fluidics & Stream Startup Fill sheath tank and empty waste. Sheath=1xPBS. To prepare, combine 200 mL of 10XPBS with 1800 mL of dH2O. For Aria-7: Tue-Fri: No fluidic startup needed. Remove waste container and add bleach to bring it to a final concentration of 10% and leave it to the side.
- Page 4 3. Cleaning Sort Chamber Clean interior chamber as well as plates and sort chamber with ethanol. Make sure all surfaces are dry. Flow cell. Check for fluid or salt buildup after removing closed loop nozzle and before inserting sort nozzle. High voltage deflection plates.
- Page 5 Note: If incorrect nozzle size shown FACSAria™ Nozzle Swap SOP for further instructions. Check the stream image and droplet formation. Confirm with images below to ensure that the droplet formation is normal.
- Page 6 Create a new tube and name it with the date: yyyymmdd Run the CS&T beads in the 3 peak experiment To prepare CS&T beads, add one drop of BD CS&T beads, Cat#655050, into 350uL of 1xPBS. (Refrigerate after use.) Verify that all channels have 3 peaks and brightest peak is falling within target.
- Page 7 Fanning of center stream seen. drop optimized. Tight center stream Open Accudrop Experiment in QC Folder See FACSAria™ Drop-Charge Delay SOP Select the tube under the specimen and open the Drop Delay Sort Layout under the Global Sheet.
- Page 8 “Fine Tune” selected Load & Run Accudrop beads. To prepare Accudrop beads, put one drop of BD Accudrop beads, Cat#345249, into 700uL of 1xPBS. (Refrigerate after use.) Acquire the beads and run at a flow rate of ~2,000 evts/sec. Carry out Accudrop sort.
- Page 9 6. Post Sort Cleanup and Shutdown Clean the sorter after appointment is complete and check to see if fluidics and/or cytometer shutdown is necessary. Before 5pm (business weekdays only): Clean with 2 minutes CLEAN (bleach) at flow rate 11, 2 minutes RINSE (detergent) at flow rate 11, followed by 2 minutes H2O at flow rate 11 and leave instrument on.
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