Page 1 thermo scientific Vanquish Diode Array/Multiple- Wavelength Detectors VC-D11, VC-D12, VF-D11 Operating Manual 4820.8302-EN Revision 2.0 • December 2019...
Page 2 Copyright © 2019 Thermo Fisher Scientific Inc. All rights reserved. Original Operating Manual The hardware descriptions in this manual revision refer to devices VC-D11-A-01, VC-D12-A-01, VF-D11-A-01. Trademarks Acrobat, Adobe, and Adobe Reader are trademarks of Adobe Systems Incorporated. Microsoft and Windows are trademarks of Microsoft Corporation. Torx is a trademark of Acument Intellectual Properties, LLC.
Page 3 Contacting Us Contacting Us There are several ways to contact us: Ordering Information For ordering information or sales support for HPLC products, contact your local Thermo Fisher Scientific sales organization. For contact information, go to Contact Us on http://www.thermofisher.com. Technical Assistance For technical support for HPLC products, contact your local Thermo Fisher Scientific support organization.
Page 4 Contacting Us Page 4 Diode Array/Multiple-Wavelength Detectors (VC-D11, VC-D12, VF-D11) Operating Manual...
Page 5: Table Of Contents
Contents Contents 1 Using this Manual .............. 11 About this Manual .................... 12 Conventions...................... 13 1.2.1 Safety Messages.................. 13 1.2.2 Special Notices and Informational Notes .......... 13 1.2.3 Typographical Conventions .............. 14 Reference Documentation ................... 15 2 Safety.................. 17 Safety Symbols and Signal Words................. 18 2.1.1 Safety Symbols and Signal Words in This Manual ........
Page 6 Contents Leak Detection...................... 40 Operation ...................... 41 4 Unpacking .................. 43 Unpacking...................... 44 Scope of Delivery.................... 47 5 Installation................. 49 Safety Guidelines for Installation ................. 50 Installing the Device ..................... 51 Site Requirements .................... 53 5.3.1 Power Considerations ................ 53 5.3.2 Power Cord.................... 54 5.3.3 Condensation ................... 54 Accessing the Interior Components .............. 55 Setting Up the Hardware.................. 56 5.5.1...
Page 7 Contents Power On/Off Control .................. 87 Preparing the Device for Operation .............. 88 6.5.1 Guidelines for Use of Flow Cells ............... 90 6.5.2 Guidelines for Use of the Lamps .............. 93 Important Operating Parameters................. 95 Optimizing the Performance of the Device ............ 99 6.7.1 General Guidelines ...................
Page 8 Contents 7.11 Updating the Device Firmware................ 144 7.12 Replacing the Doors................... 146 7.13 Transporting or Shipping the Device .............. 148 7.13.1 Preparing the Device for Transport............ 148 7.13.2 Transporting the Device to a New Location ........... 149 7.13.3 Shipping the Device................ 150 7.14 Replacing the Slide-In Module................
Page 9 Contents 11.2 UV Cutoff Wavelengths of Solvents.............. 185 Index.................. 187 Diode Array/Multiple-Wavelength Detectors (VC-D11, VC-D12, VF-D11) Page 9 Operating Manual...
Page 10 Contents Page 10 Diode Array/Multiple-Wavelength Detectors (VC-D11, VC-D12, VF-D11) Operating Manual...
Page 11: Using This Manual
• Using this Manual 1 Using this Manual This chapter provides information about this manual, the conventions used throughout the manual, and the reference documentation that is available in addition to this manual. Diode Array/Multiple-Wavelength Detectors (VC-D11, VC-D12, VF-D11) Page 11 Operating Manual...
Page 12: About This Manual
• Using this Manual About this Manual This manual describes the functional features and operating principle of your Vanquish™ device and provides instructions for installation, set up, start up, shut down, operation, maintenance and troubleshooting. The layout of this manual is designed to provide quick reference to the sections of interest to the user.
Page 13: Conventions
• Using this Manual Conventions This section describes the conventions that are used throughout this manual. 1.2.1 Safety Messages The safety messages and precautionary statements in this manual appear as follows: • Safety messages or precautionary statements that apply to the entire manual and all procedures in this manual are grouped in the Safety chapter.
Page 14: Typographical Conventions
• Using this Manual 1.2.3 Typographical Conventions These typographical conventions apply to the descriptions in this manual: Data Input and Output • The following appears in bold type: Input that you enter by the keyboard or that you select with the ¨...
Page 15: Reference Documentation
• Using this Manual Reference Documentation In addition to this operating manual, other documentation is available for reference. Hardware Documentation Additional hardware documentation includes the following: • Operating manuals for the other modules of the Vanquish system A printed version of the manual is shipped with the device. •...
Page 16 • Using this Manual TIP The Chromeleon Help and documents are included in the software shipment. Third-Party Documentation Refer also to the user documentation provided by the manufacturers of third-party components and materials, for example, Safety Data Sheets (SDSs). Page 16 Diode Array/Multiple-Wavelength Detectors (VC-D11, VC-D12, VF-D11) Operating Manual...
Page 17: Safety
• Safety 2 Safety This chapter provides general and specific safety information and informs about the intended use of the device. Diode Array/Multiple-Wavelength Detectors (VC-D11, VC-D12, VF-D11) Page 17 Operating Manual...
Page 18: Safety Symbols And Signal Words
• Safety Safety Symbols and Signal Words 2.1.1 Safety Symbols and Signal Words in This Manual This manual contains safety messages to prevent injury of the persons using the device. The safety symbols and signal words in this manual include the following: Always be aware of the safety information.
Page 19: Safety Symbols On The Device
• Safety 2.1.3 Safety Symbols on the Device The table lists the safety symbols that appear on the device or on labels affixed to the device. Follow the safety notices in this manual to prevent the risk of operator injury or damage to the device. Symbol Description Indicates a potential hazard.
Page 20: Intended Use
• Safety Intended Use The device is intended to be part of the Vanquish system. The intended use of the Vanquish system is to analyze mixtures of compounds in sample solutions. The device is for use by qualified personnel and in laboratory environment only.
Page 21: Safety Precautions
• Safety Safety Precautions 2.3.1 General Safety Information All users must observe the general safety information presented in this section and all specific safety messages and precautionary statements elsewhere in this manual during all phases of installation, operation, troubleshooting, maintenance, shutdown, and transport of the device. If the device is used in a manner not specified by Thermo Fisher Scientific, the protection provided by the device could be impaired.
Page 22: Qualification Of The Personnel
• Safety 2.3.2 Qualification of the Personnel Observe the information below on the proper qualification of the personnel installing and/or operating the device. Installation Only skilled personnel are permitted to install the device and to establish the electrical connections according to the appropriate regulations.
Page 23: Electrical Safety Precautions
• Safety Protective Eyewear To prevent liquids from striking your eyes, put on appropriate protective eyewear, such as safety glasses with side shields. If there is a risk of splashing liquids, put on goggles. Gloves To protect you from harmful liquids and avoid personal injury during maintenance or service, put on appropriate protective gloves.
Page 24: General Residual Hazards
• Safety 2.3.5 General Residual Hazards Pay attention to the following general residual hazards when working with the device: WARNING—Hazardous Substances Solvents, mobile phases, samples, and reagents might contain toxic, carcinogenic, mutagenic, infectious, or otherwise harmful substances. The handling of these substances can pose health and safety risks. •...
Page 25 • Safety WARNING—Self-Ignition of Solvents Solvents with a self-ignition temperature below 150 °C might ignite when in contact with a hot surface (for example, due to leakage in the chromatography system). Avoid the use of these solvents. WARNING—Hazardous Vapors Mobile phases and samples might contain volatile or flammable solvents.
Page 26: In Case Of Emergency
• Safety CAUTION—Sparking due to Electrostatic Discharge Liquid flowing through capillaries can generate static electricity. This effect is particularly present with insulating capillaries and non- conductive solvents (for example, pure acetonitrile). Discharge of electrostatic energy might lead to sparking, which could constitute a fire hazard.
Page 27: Solvent And Additive Information
• Safety Solvent and Additive Information 2.4.1 General Compatibility To protect optimal functionality of the Vanquish system, observe these recommendations on the use of solvents and additives: • The system must be used with reversed-phase (RP) compatible solvents and additives only. •...
Page 28: Allowed Ph Ranges
• Safety 2.4.2 Allowed pH Ranges Allowed pH ranges (standard system configuration): System Allowed Remarks (Standard pH ranges Configuration) Vanquish Core 1-13 • pH values of 2 or less: The application time should be as short as possible. Flush the system Vanquish 2-12 thoroughly after these applications.
Page 29: Further Information
• Safety 2.4.4 Further Information • For details about the materials that are used in the analytical flow path of the device, see the Specifications chapter in this manual. For information about the materials that are used in the flow path of the other modules in the Vanquish system, refer to the Specifications chapter in the Operating Manual for the modules.
Page 30: Compliance Information
• Safety Compliance Information Thermo Fisher Scientific performs complete testing and evaluation of its products to ensure full compliance with applicable domestic and international regulations. When the device is delivered to you, it meets all pertinent electromagnetic compatibility (EMC) and safety standards as described in this manual.
Page 31: Device Overview
• Device Overview 3 Device Overview This chapter introduces you to the device and the main components. Diode Array/Multiple-Wavelength Detectors (VC-D11, VC-D12, VF-D11) Page 31 Operating Manual...
Page 32: Device Features
• Device Overview Device Features The device comprises the following main features: Wavelength Detection Range and Lamps Characteristic Values Wavelength detection 190 nm to 800 nm range Light sources Deuterium lamp For ultraviolet (UV) detection Tungsten lamp For visible (VIS) and near-infrared (NIR) detection (standard feature for the VF detector, optional feature for the VC detectors)
Page 33 • Device Overview Motorized Filter Paddle The filter paddle automatically moves into the following positions: Optimization Features Purpose Open For data acquisition Closed (dark) For performing autozero Holmium oxide filter For validation of wavelength accuracy Optional Accessories • Various flow cells for different fields of applications •...
Page 34: Operating Principle
• Device Overview Operating Principle The device is designed for ultraviolet (UV), visible (VIS) and near-infrared (NIR) absorption spectroscopy in combination with HPLC or UHPLC separations. After sufficient separation from other sample compounds, the analysis of the target compound follows the Beer-Lambert law. This means that the response of the detector is proportional to the: •...
Page 35 • Device Overview A tungsten lamp (1) as light source emits VIS and NIR light. The VIS lens (2) focuses the light through an opening in the internal structure of the deuterium lamp (3) that emits UV light. The achromat (4) focuses the light beams from both lamps so that the beams pass through the flow cell (5).
Page 36: Interior Components
• Device Overview Interior Components The user-accessible components of the device are located directly behind the front doors: Figure 2: Interior view with flow cell installed Description Cooling air intake Keypad with status indicators Lamp house cover Flow cell (after installation) LED bar Connection unit Leak tray...
Page 37: Flow Cell
• Device Overview Flow Cell The detector design allows easy access to the flow cell on the interior front. Figure 3: Flow cell (example) Description ID Chip Flow cell body Optical port (second optical port is on the opposite side) Connection unit Outlet (OUT) (closed with plug during storage and transport) Inlet (IN) (closed with plug during storage and transport) Handle...
Page 38 • Device Overview detector reads the data from the chip and transfers the flow cell data to Chromeleon. Closing the front door triggers an immediate flow cell installation check. Heat exchanger The flow cells are equipped with a built-in heat exchanger. The heat exchanger helps to adapt the temperature of the mobile phase to the flow cell temperature before the mobile phase enters the optical flow path within the flow cell.
Page 39: Lamps
• Device Overview Lamps Lamp Types Two different light sources provide the light for a wavelength detection range from 190 nm to 800 nm: • Deuterium lamp for ultraviolet (UV) wavelength detection • Tungsten lamp for visible (VIS) and near-infrared (NIR) wavelength detection (standard for the VF detector, optional for the VC detectors) The lamp house containing both lamps is temperature controlled.
Page 40: Leak Detection
• Device Overview Leak Detection Leaks are a potential safety issue. The leak sensor inside the device monitors the device for liquid leaks from the flow connections. The liquid is collected in the leak tray and guided to the drain port. From the drain port, the liquid is discharged to waste through the drain system of the Vanquish system.
Page 41: Operation
• Device Overview Operation The device is designed to be operated from a computer configured with the Chromeleon Chromatography Data System (CDS). The Chromeleon software provides complete instrument control, data acquisition, and data management. For a basic description of instrument control and automated sample analysis with the Chromeleon software, refer to the Vanquish System Operating Manual.
Page 42 • Device Overview Page 42 Diode Array/Multiple-Wavelength Detectors (VC-D11, VC-D12, VF-D11) Operating Manual...
Page 43: Unpacking
• Unpacking 4 Unpacking This chapter provides information for unpacking the device and informs you about the scope of delivery. Diode Array/Multiple-Wavelength Detectors (VC-D11, VC-D12, VF-D11) Page 43 Operating Manual...
Page 44: Unpacking
• Unpacking Unpacking Damaged Packaging, Defective on Arrival Inspect the shipping container for signs of external damage and, after unpacking, inspect the device for any signs of mechanical damage that might have occurred during shipment. If you suspect that the device may have been damaged during shipment, immediately notify the incoming carrier and Thermo Fisher Scientific about the damage.
Page 45 • Unpacking Follow these steps 1. Place the shipping container on the floor and open it. 2. Remove the ship kit from the shipping container. 3. Remove the device from the shipping container: Grasp the device by the carrying handles. Slowly and carefully, lift the device out of the shipping container.
Page 46 • Unpacking 8. Slide off the carrying handles from the rails towards the rear of the device. Figure 5: Sliding off the carrying handle from the left rail TIP Keep the shipping container, the carrying handles with the attachment screws, and all packing material. These items will be needed if the device is transported to a new location or shipped.
Page 47: Scope Of Delivery
• Unpacking Scope of Delivery The following items are included in the delivery: • Detector • Ship Kit • Operating manual • Power cord For information on contents of the ship kit or reordering parts, see Accessories, Consumables and Replacement Parts (} page 175).
Page 48 • Unpacking Page 48 Diode Array/Multiple-Wavelength Detectors (VC-D11, VC-D12, VF-D11) Operating Manual...
Page 49: Installation
• Installation 5 Installation This chapter specifies the requirements for the installation site and describes how to set up, install, and configure the device in the Vanquish system and in the chromatography software. Diode Array/Multiple-Wavelength Detectors (VC-D11, VC-D12, VF-D11) Page 49 Operating Manual...
Page 50: Safety Guidelines For Installation
• Installation Safety Guidelines for Installation Pay attention to the following safety guidelines: Observe all warning messages and precautionary statements presented Safety Precautions (} page 21). CAUTION—Heavy Load, Bulky Device The device is too heavy or bulky for one person alone to handle safely. To avoid personal injury or damage to the device, observe the following guidelines: •...
Page 51: Installing The Device
• Installation Installing the Device The Vanquish system is installed and set up by a Thermo Fisher Scientific service engineer, including all modules and options or parts shipped with them. The service engineer checks that the installation is correct and that the Vanquish system and modules operate as specified.
Page 52 • Installation 6. Recommended: Perform Instrument Installation Qualification. In the Chromeleon software, a wizard is available to guide you through the qualification process. On the Chromeleon 7 Console: Click Tools > Instrument Qualification > Installation Qualification. Follow the instructions in the Instruments Installation Qualification Operating Instructions.
Page 53: Site Requirements
• Installation Site Requirements The operating environment is important to ensure optimal performance of the device. This section provides important requirements for the installation site. Note the following: • Operate the device only under appropriate laboratory conditions. • The device is intended to be part of the Vanquish system. Observe the site requirements for the Vanquish system as stated in the Vanquish System Operating Manual.
Page 54: Power Cord
• Installation 5.3.2 Power Cord The power cords are designed to match the wall socket requirements of the country in which they are used. The end of the power cords that plugs into the power socket on the device is identical for all power cords. The end of the power cords that plugs into the wall socket is different.
Page 55: Accessing The Interior Components
• Installation Accessing the Interior Components To access the interior components in the device, open the front doors. To allow easy access from the front, the user-accessible components and flow connections in the device are located directly behind the doors. Figure 6: Opening the front doors Diode Array/Multiple-Wavelength Detectors (VC-D11, VC-D12, VF-D11) Page 55...
Page 56: Setting Up The Hardware
• Installation Setting Up the Hardware This section describes how to set up the hardware and provides information about the device connectors and cables. 5.5.1 System Arrangement The device is part of the Vanquish system. The system modules are typically arranged in a system stack, with the arrangement depending on the system configuration.
Page 57: Connecting The Device
• Installation 5.5.2 Connecting the Device Device Connectors The following connectors are provided on the device: Figure 8: Electrical connectors on the right side of the device Description Rating plate, indicating the serial number, part number, module name, revision number (if any), line and fuse rating, and the manufacturer's address Main power switch (on/off control) Fuse holder Power inlet connector...
Page 58: Connecting The Power Cord
• Installation Description USB (Universal Serial Bus) port ("B" type connector) Allows connection to other modules in the Vanquish system or the computer on which the data management system is installed, such as the Chromeleon software TIP Thermo Fisher Scientific recommends using the USB ports only as described above.
Page 59: Installing A Vis Lamp (Tungsten Lamp) (Optional)
• Installation Installing a VIS Lamp (Tungsten Lamp) (Optional) Follow the instructions below to install the optional VIS lamp. Parts required VIS lamp Preparations 1. Turn off the device. Follow these steps Figure 9: VIS lamp Description VIS lamp connector VIS lamp VIS lamp attachment screw VIS lamp locating pin Diode Array/Multiple-Wavelength Detectors (VC-D11, VC-D12, VF-D11)
Page 60 • Installation 1. Open the doors. 2. Turn the two screws on the lamp house cover counterclockwise until the lamp house cover is loose. Do not remove the screws completely from the lamp house cover. Figure 10: Screws on the lamp house cover 3.
Page 61 • Installation 11. If you install a VIS lamp for the first time and the detector is operated with Chromeleon, activate the lamp in Chromeleon: Only then, the VIS lamp can be ignited from Chromeleon. a) Right-click the detector in the Instrument Configuration Manager.
Page 62: Installing A Flow Cell
• Installation Installing a Flow Cell This section describes the installation of the flow cell upon initial installation of the device. NOTICE Flow cells are sensitive to dirt and dust. Observe the following notes when installing the flow cell to the detector: •...
Page 63 • Installation Follow these steps 1. Open the device doors. 2. Loosen the two screws of the flow cell cover, remove the cover and keep the cover to close the flow cell opening after this procedure. CAUTION—UV Radiation The deuterium lamp emits UV radiation that is harmful to eyes and skin. Do not look and do not reach into the flow cell opening while no flow cell or diagnostic cell is installed and the UV lamp is turned on.
Page 64 • Installation 4. Flow cells with connection unit only: Press the locating pin of the connection unit into the slot provided on the interior front panel. Figure 12: Installing a flow cell with connection unit Description Connection unit Connection unit screw Locating pin Connection unit socket Slot for locating pin...
Page 65 • Installation 5. Flow cells with connection unit only: Tighten the screw of the connection unit hand-tight. 6. To ensure best performance, make sure that the flow cell is properly installed: Check against the flow cell positioning marks (no. 1 in the figure below) whether the flow cell is seated correctly.
Page 66 • Installation 8. Mount the flow cell cover so that the slot in the flow cell cover is located on the bottom right (see image). Make sure that the capillaries run through the slot in the flow cell cover. Figure 14: Fixing the flow cell cover (here: flow cell with connection unit) 9.
Page 67: Setting Up The Flow Connections
• Installation Setting Up the Flow Connections 5.8.1 General Information and Guidelines When setting up flow connections, follow these rules and recommendations: Flow connections can be filled with hazardous substances. Observe the warning messages and precautionary statements presented in Safety Precautions (} page 21).
Page 68 • Installation NOTICE Flow cells are sensitive to contamination, clogging and high backpressures. If the pressure exceeds the upper limit, the flow cell may be permanently damaged. Observe the following notes when connecting the flow cell to the system flow path: •...
Page 69: Guiding Capillaries And Tubing Through The System
• Installation Follow these steps To set up the flow connections and complete the installation of the device, follow these steps: 1. Set up the flow connections to the flow cell (see Flow Connections to the Flow Cell (} page 74)). 2. Connect the device to the drain system (refer to the Vanquish System Operating Manual).
Page 70: Installing The Partition Panel Plugs
• Installation Tubing brackets are available for holding the tubing in place. Slip the bracket side onto the drain pipe. Figure 16: Tubing bracket (left), tubing bracket installed (right) Dual System Arrangements The number of tubes may exceed the capacity that the tubing guides can hold.
Page 71 • Installation Installing the plug with slit 1. On the detector partition panel, push the plug with slit in the required recess of the partition panel (if not present yet). 2. To secure the plug with slit, insert the nose into the opening of the partition panel.
Page 72: Connecting Fittings, Capillaries, And Tubing
• Installation Installing the rotating plug 1. On the detector partition panel, push the rotating plug in the required recess of the partition panel (if not present yet). 2. To open the plug in order to route the capillary through the plug, turn the rotating plug toward the front.
Page 73 • Installation 5.8.4.2 Connecting Viper Capillaries This section describes how to connect Viper™ capillaries. All Viper flow connections in the Vanquish system are designed to be finger-tight. To connect Viper capillaries with knurls, follow these steps: NOTICE • Tighten or loosen Viper capillaries only with your fingers. Do not use tools other than the knurl that comes with the capillary.
Page 74: Flow Connections To The Flow Cell
• Installation Resolving Leakage of Viper Fittings with Knurls 1. Tighten the connection a little more. 2. If leakage continues, remove the capillary. 3. Clean the capillary ends carefully by using a lint-free tissue wetted with isopropanol. 4. Reinstall the capillary. 5.
Page 75 • Installation Follow these steps 1. Connect the inlet capillary to the flow cell. 2. Connect the detector waste line to the flow cell. See also Connecting the Inlet Capillary (} page 75) Connecting the Detector Waste Line (} page 76) 5.8.5.1 Connecting the Inlet Capillary Preparations Flow Connections to the Flow Cell (} page 74).
Page 76 • Installation 2. If the column compartment is located to the right of the detector Route the capillary through the bottom recess in the partition panel. Make sure that you use the suitable partition panel plug for the capillary. TIP For capillaries with small outer diameter, the plug with slit secures the capillary properly.
Page 77 • Installation 4. On the system base, route the detector waste line through the dedicated detector waste outlet to waste and connect the detector waste line to the waste container as described in the Vanquish System Operating Manual. The waste line should go straight to the system base and to waste. Make sure that the line is positioned straight in the tubing guides.
Page 78: Guiding Liquid Leaks To Waste
• Installation 5.8.6 Guiding Liquid Leaks to Waste Leaking liquids of the device are collected in the leak tray, where they flow off through the chase on the right side of the leak tray to the drain system. For information about how the liquid is discharged to waste through the Vanquish drain system, refer to the Vanquish System Operating Manual.
Page 79: Turning On The Device
• Installation Turning On the Device Before turning on the power to a Vanquish system module for the first time, verify that the chromatography software is installed on the data system computer. When the power is turned on, the required USB drivers are automatically found and the Windows™...
Page 80: Setting Up The Device In The Software
• Installation 5.10 Setting Up the Device in the Software This manual assumes that the chromatography software is already installed on the data system computer and a valid license is available. For more information about setting up the Vanquish system in the software, refer to the Vanquish System Operating Manual.
Page 81: Operation
• Operation 6 Operation This chapter describes the elements for device control, provides information for routine operation and for shutdown. Diode Array/Multiple-Wavelength Detectors (VC-D11, VC-D12, VF-D11) Page 81 Operating Manual...
Page 82: Introduction To This Chapter
• Operation Introduction to this Chapter The information in this chapter assumes that the initial setup of the device has already been completed. If this is not the case, refer to the instructions in Installation (} page 49). For a basic description of instrument control and automated sample analysis with the Chromeleon software, refer to the Vanquish System Operating Manual.
Page 83: Safety Guidelines For Operation
• Operation Safety Guidelines for Operation When operating the device, pay attention to the following safety guidelines: Observe all warning messages and precautionary statements presented Safety Precautions (} page 21). CAUTION—Hot Surfaces Surfaces inside the device may become hot during operation. Touching hot parts might cause burns.
Page 84: Control Elements
• Operation Control Elements The device is designed to be operated mainly from a computer running with the chromatography software. In addition, the following elements are available on the device: • Keypad The keypad buttons allow you to perform certain functions directly from the device.
Page 85 • Operation MUTE ALARM Beeping alerts you when the device detects a problem, for example, leakage. To turn off the beep for the current alarm, press this button. Eliminate the source for the alarm within 10 minutes. Otherwise, beeping starts again. If the device detects a different problem, beeping alerts you again immediately.
Page 86: Status Indicators
• Operation 6.3.2 Status Indicators The status LED bar on the front side of the device and the STATUS LED on the inside keypad provide information about the device status. LED Bar The LED bar provides the information when the device is closed. When the device is connected in the Chromeleon software, the LED bar may provide less information.
Page 87: Power On/Off Control
• Operation Power On/Off Control The power switch on the device is the main switch for power on/off control. The main power switch is turned on during initial installation of the device. For easier handling, you can use the power button on the front left of the Vanquish system base (system power button) for power on/off.
Page 88: Preparing The Device For Operation
• Operation Preparing the Device for Operation This section gives information on any additional steps that are required to prepare the device for operation and sample analysis. Before Operating the Device for the First Time Prepare the device for the first-time operation, observing the following: NOTICE Flush the system flow path thoroughly before operating the device for the first time:...
Page 89 • Operation System Equilibration System equilibration should include the following operations: • Purging the pump (all channels, including those not used for the application) • Flushing the entire chromatography system with the starting solvent to rinse out any solvent from a previous analysis run •...
Page 90: Guidelines For Use Of Flow Cells
• Operation 6.5.1 Guidelines for Use of Flow Cells NOTICE—Sensitive flow cells Flow cells are sensitive to damage and contamination. • Handle flow cells with care. • Observe the following guidelines for use of the flow cell. Operating conditions Make sure the following conditions are met while operating the flow cell: •...
Page 91 • Operation In the capillaries: ¨ Can lead to a blockage of the capillary. A blocked waste line can lead to flow cell damage. To avoid this, observe the following: Use highly pure solvents, such as of LC/MS-grade, only. ¨ If capillaries are removed from the flow cell inlet and outlet, ¨...
Page 92 • Operation Interrupted Operation • During short analysis breaks, take appropriate measures to protect the flow cell: Never leave any substances in the flow cell without flow. Avoid ¨ leaving particularly any aggressive solvents in the flow cell for a longer time.
Page 93: Guidelines For Use Of The Lamps
• Operation 6.5.2 Guidelines for Use of the Lamps The table shows which lamp is recommended if at least one of your detection wavelength is within the range indicated in the left column. The values apply to single-channel and 3D data field measurements. At least one detection wavelength Recommended lamp ≤ 345 nm...
Page 94 • Operation 6.5.2.2 Turning off the Lamps UV Lamp You can turn off the UV lamp using the UV button on the keypad of the device or using Chromeleon (see Important Operating Parameters (} page 95)). It is not possible to turn the UV lamp off or on during data acquisition.
Page 95: Important Operating Parameters
• Operation Important Operating Parameters The commands and parameters described in the table should be considered for simple routine operation of the device. You can usually access these parameters from the Chromeleon user interface. If a parameter listed below is not available in the Chromeleon software, consider updating the firmware and Chromeleon version.
Page 96 • Operation Parameters Description Wavelength Sets a wavelength for each of the UV_VIS_x signal channels individually, in the range of 190 nm to 800 nm. The wavelength defines the value at which the detector measures the absorbance of the analyte(s) in the sample. Observe the following: •...
Page 97 • Operation Parameters Description Response time The response time determines how quickly the detector responds to a change in signal. As a standard, the Response Time setting is defined when the data collection rate is set in the Instrument Method Wizard.
Page 98 • Operation Parameters Description Bunch width (only If the 3D Field signal is selected, set the bunch width. available for the VF‑D11 The bunch width setting determines the distance of the and VC‑D11 detectors) wavelengths between recorded data points in a 3D field. For each recorded data point, the absorption of a diode range is averaged (bunched) with the width of the bunch width.
Page 99: Optimizing The Performance Of The Device
• Operation Optimizing the Performance of the Device This section provides information for best performance of the device and gives hints on what you can do to optimize the performance further. See also Important Operating Parameters (} page 95) 6.7.1 General Guidelines Consider the following guidelines for optimization of the device performance: •...
Page 100: Overview Of Optimization Parameters
• Operation • Observe the general guidelines and recommendations on the use of solvents and additives in the chromatography system. Refer to Use of Solvents and Additives in the Vanquish System Operating Manual. • Degas the solvent. • Consider the influences of the parameters on the detection. •...
Page 101 • Operation 6.7.2.1 Selecting the Data Collection Rate The data collection rate is the number of data points per second (Hz) that the Chromeleon software collects from the detector and stores as raw data. When you select a data collection rate, observe the following guidelines: •...
Page 102 • Operation 6.7.2.2 Selecting the Response Time and Peak Width As a standard, when setting the data collection rate, Chromeleon automatically sets the optimum response time and peak width. As an advanced option, response time and peak width can be set individually. Disabling the link between the data collection rate parameters and setting these parameters individually may lead to increased noise or increased peak widths.
Page 103 • Operation Bandwidth The bandwidth specifies the spectral bandwidth at which a chromatogram (UV_VIS channel) is recorded. You can accept the standard bandwidth in Chromeleon, or set the bandwidth to a higher or lower value. Setting a wider bandwidth will average more photodiode signals.
Page 104 • Operation Slit Width (only applicable to the VF detector) You can select between two different slit widths: • Narrow • Wide The following table shows the differences of these two slit widths. Characteristic Narrow Wide Spectral bandwidth Smaller Higher Spectral resolution (the Higher Lower ability of the detector to...
Page 105 • Operation Reference Wavelength Interfering substances can absorb in addition to the absorption of the analyte in the sample. The absorption of the interferences adds to the measured signal from the analyte. As a result, this can lead to errors in the measurement of the analyte concentration.
Page 106 • Operation Reference Bandwidth If the reference wavelength is used, the reference bandwidth determines how many photodiode signals are averaged for the reference measurement. You can set the reference bandwidth separately for each signal channel and the 3D field (only available for the VC‑D11 and VF‑D11 detectors).
Page 107 • Operation 6.7.2.5 Baseline Behavior If wavelengths are switched during a data acquisition, the chromatogram may show baseline jumps. You can define how the baseline behaves in these cases: • Zero: sets the baseline to zero • Append: appends the baseline to the previous signal •...
Page 108: Shutting Down The Device
• Operation Shutting Down the Device If the device will not be operated for some time, follow the instructions in this section to shut down the device. TIP The Chromeleon software provides procedures for automatically preparing the chromatography system for shutdown. The procedures include, for example, operations for reducing the flow rate, reducing the temperature in temperature-controlled devices, and turning off the detector lamps.
Page 109: Long-Term Shutdown
• Operation NOTICE Turning on and off the UV lamp too often can reduce the lifetime of the UV lamp. • Do not turn off the UV lamp if you intend to use the detector again within 24 hours. If you want to use the detector the next day, leave the UV lamp turned on and operate the detector with a flow rate of 0.05 mL/min.
Page 110 • Operation 6. Flush the flow cell with an appropriate solvent (minimum HPLC- grade). Observe the following: TIP With a Vanquish Core system that has been modified for using normal-phase compatible solvents and additives, see the information about the flushing liquid in the Considerations with Normal-Phase Compatible Solvents and Additives section in Operating Manual for the pump.
Page 111 • Operation NOTICE Residual samples, impurities from the column or buffers with high salt concentrations can deposit in the flow cell. This can lead to damage of the flow cell. In addition, solvents containing acid can damage the flow cell. •...
Page 112: Restart After Long-Term Shutdown
• Operation 6.8.3 Restart after Long-Term Shutdown To restart the device after a long-term shutdown, follow these steps: 1. Prepare and restart the other modules in the Vanquish system, following the instructions in the Operating Manuals for the modules. Pay special attention to the Preparing the Module for Operation section.
Page 113: Maintenance And Service
• Maintenance and Service 7 Maintenance and Service This chapter describes the routine maintenance and the service procedures that the user may perform. Diode Array/Multiple-Wavelength Detectors (VC-D11, VC-D12, VF-D11) Page 113 Operating Manual...
Page 114: Introduction To Maintenance And Service
• Maintenance and Service Introduction to Maintenance and Service This chapter describes the routine maintenance and service and repair procedures that the user may perform. Additional maintenance or service procedures must be performed only by service personnel certified by Thermo Fisher Scientific (for brevity, referred to as Thermo Fisher Scientific service personnel).
Page 115: Safety Guidelines For Maintenance And Service
• Maintenance and Service Safety Guidelines for Maintenance and Service 7.2.1 General When performing maintenance or service procedures, pay attention to the following safety guidelines: Observe all warning messages and precautionary statements presented Safety Precautions (} page 21). WARNING—High Voltage High voltages are present inside the device that could cause an electric shock.
Page 116 • Maintenance and Service CAUTION—Spraying Solvent Solvents can spray when under high pressure. • Stop the pump flow prior to opening the flow path. • Wait until the system pressure is down to zero. • When opening the flow path, wear appropriate protective equipment.
Page 117: Flow Cells
• Maintenance and Service CAUTION—Electric Shock or Damage to the Device After the power to the device is turned off, the device is still energized as long as the power cord is connected. Repair work on the device while the device is connected to power could lead to personal injury. •...
Page 118: General Rules For Maintenance And Service
• Maintenance and Service General Rules for Maintenance and Service For successful maintenance and service procedures, follow these rules and recommendations: • Before starting maintenance or service procedures, shut down the device when instructed to do so. • Use only the replacement parts specifically authorized and qualified for the device by Thermo Fisher Scientific.
Page 119: Routine And Preventive Maintenance
• Maintenance and Service Routine and Preventive Maintenance Optimum device performance, maximum uptime of the device, and accurate results can be obtained only if the device is in good condition and properly maintained. 7.4.1 Maintenance Plan Perform the maintenance procedures in the table on a regular basis. The frequency given in the table is a suggestion.
Page 120 • Maintenance and Service Decontamination Decontamination is required, for example, when leakage or spillage has occurred, or before service or transport of the device. Use a suitable cleaning detergent or disinfectant to ensure that the treatment renders the device safe to handle. Parts required •...
Page 121: Predictive Performance
• Maintenance and Service Preparations 1. Turn off the power to the device and disconnect the power cord from the power source. Follow these steps 1. Wipe the surfaces clean with a clean, dry, soft, lint-free cloth or wipe. If necessary, slightly dampen the cloth or wipe with a solution of lukewarm water and a suitable cleaning detergent.
Page 122: Performing A Wavelength Validation And Calibration
• Maintenance and Service Performing a Wavelength Validation and Calibration A holmium-oxide glass filter is used to validate the wavelength accuracy. The filter can be moved into the light path in front of the entrance slit for the wavelength validation. The detector determines the wavelengths of several absorption peaks in the resulting spectrum and compares them to the nominal holmium oxide values that are stored in the detector firmware.
Page 123 • Maintenance and Service To perform a wavelength validation 1. Turn on the flow at the flow rate that will be used for the analysis. 2. Perform the wavelength validation from Chromeleon. 3. Check whether the wavelength validation passed. If the wavelength validation fails, perform a wavelength calibration from Chromeleon (see next section).
Page 124: Lamps
• Maintenance and Service Lamps This section describes the replacement of these lamps: • UV lamp (see Replacing the UV Lamp (Deuterium Lamp) (} page 124)) • VIS lamp (see Replacing the VIS Lamp (Tungsten Lamp) (} page 128)) NOTICE—Damage to the device Usage of lamps not supplied or approved by Thermo Fisher Scientific for this detector may result in inferior performance, detector malfunction or may damage the detector.
Page 125 • Maintenance and Service Follow these steps Figure 27: UV lamp Description UV lamp connector UV lamp UV lamp attachment screw UV lamp locating pin Cable clip Diode Array/Multiple-Wavelength Detectors (VC-D11, VC-D12, VF-D11) Page 125 Operating Manual...
Page 126 • Maintenance and Service 1. Open the doors. 2. Turn the two screws on the lamp house cover counterclockwise until the lamp house cover is loose. Do not remove the screws completely from the lamp house cover. Figure 28: Screws on the lamp house cover 3.
Page 127 • Maintenance and Service 9. Align the new lamp with the locating pin. When the lamp is in the correct position, push the lamp gently into the lamp house. The lamp socket must be in a level position with the lamp house.
Page 128: Replacing The Vis Lamp (Tungsten Lamp)
• Maintenance and Service 7.6.2 Replacing the VIS Lamp (Tungsten Lamp) When • The lamp is defective • After a defined amount of operating hours of the lamp as a preventive measure • The lamp intensity is too low for demanding applications •...
Page 129 • Maintenance and Service 1. Open the doors. 2. Turn the two screws on the lamp house cover counterclockwise until the lamp house cover is loose. Do not remove the screws completely from the lamp house cover. Figure 30: Screws on the lamp house cover 3.
Page 130 • Maintenance and Service 10. Mind the routing of the lamp cables. Position the lamp cables as indicated in Figure 29 VIS lamp (} page 128) to prevent the cable from being pinched under the lamp house cover. 11. Install the lamp house cover and fasten the screws on the lamp house cover.
Page 131: Flow Cell
• Maintenance and Service Flow Cell This section describes how to flush and exchange flow cells. To store or ship the flow cell, follow these steps: 1. Flush the flow cell (see Preparing the Flow Cell for Storage (} page 132)). 2. Remove the flow cell from the device and place it in its packaging (see Removing the Flow Cell (} page 134)).
Page 132: Preparing The Flow Cell For Storage
• Maintenance and Service 7.7.2 Preparing the Flow Cell for Storage When Before the flow cell is stored in its packaging or in the device if one of the following applies: • Flushing the flow cell with highly pure isopropanol through the system is not possible •...
Page 133 • Maintenance and Service 6. Push the isopropanol in the syringe into the flow cell. Make sure that no residual air in the syringe is pushed into the flow cell. 7. Disconnect the waste line from the flow cell outlet. 8.
Page 134: Cleaning The Flow Cell
• Maintenance and Service 7.7.3 Cleaning the Flow Cell When When you suspect that eluent or sample components may have deposited on the flow cell windows. Parts required • Flushing and injection kit for flow cells (optional) • HPLC-grade water •...
Page 135 • Maintenance and Service Parts required • If the flow cell is to be stored: Packaging of the flow cell • Cover for the flow cell opening on the detector. Preparations 1. Flush the flow cell with isopropanol. 2. Turn off the pump flow. Follow these steps CAUTION—Hot surface The flow cell may become hot.
Page 136 • Maintenance and Service 6. Firmly squeeze the handle of the flow cell and carefully pull it from the opening in the detector. CAUTION—UV Radiation The deuterium lamp emits UV radiation that is harmful to eyes and skin. Do not look and do not reach into the flow cell opening while no flow cell or diagnostic cell is installed and the UV lamp is turned on.
Page 137: Diagnostic Cell
• Maintenance and Service Diagnostic Cell When problems with the baseline noise, baseline drift or during wavelength validation occur, install the diagnostic cell and use it to identify the cause: • If the problems disappear with the diagnostic cell The problems are caused by the flow cell that was previously installed or the flow path or components in the flow path before the device.
Page 138 • Maintenance and Service Follow these steps 1. Hold the diagnostic cell with the diagnostic cell body to the left, firmly squeeze the handle of the diagnostic cell and insert it straight into the flow cell opening. Release the handle. If there is a click, the diagnostic cell is properly mounted.
Page 139: Removing The Diagnostic Cell
• Maintenance and Service 7.8.2 Removing the Diagnostic Cell Preparations 1. Loosen the two screws of the flow cell cover, remove the cover and keep the cover to close the flow cell opening after this procedure. Follow these steps 1. Carefully pull out the diagnostic cell from the opening in the device. CAUTION—UV Radiation The deuterium lamp emits UV radiation that is harmful to eyes and skin.
Page 140: Replacing The Waste Line
• Maintenance and Service Replacing the Waste Line NOTICE Backpressures that exceed the specified maximum pressure limit of the flow cell can destroy the flow cell. Observe the following: • Use only the waste line connection that is provided for your detector.
Page 141 • Maintenance and Service Follow these steps 1. When removing the waste line from the tubing guides, be careful not to pull on other tubing in the guides. 2. Disconnect the waste line from the flow cell outlet. 3. Remove the waste line from the top recess of the partition panel: If the waste line is installed in a rotating plug ¨...
Page 142: Replacing The Main Power Fuses
• Maintenance and Service 7.10 Replacing the Main Power Fuses When Blown fuses Parts required Fuses (2 fuses, 3.15 AT, 250 V AC, slow-blow, 5 x 20 mm) from Fuses Kit Tools required Slotted screwdriver, any size between 3.3 mm and 5.5 mm is appropriate Preparations WARNING—Electric Shock High voltages are present inside the device that could cause an electric...
Page 143 • Maintenance and Service 1. Use the screwdriver to remove the fuse holder. 2. Replace the two fuses with new fuses of the specified type and current rating. Always replace both fuses. 3. Reinstall the fuse holder. 4. Reconnect the power cord to the power source and to the device. 5.
Page 144: Updating The Device Firmware
• Maintenance and Service 7.11 Updating the Device Firmware When Updating the device firmware might be required, for example, when a new firmware version is released that adds functionality or solves problems of a previous version. Items required Firmware version/Chromeleon version as appropriate TIP When a new firmware version is released, the new version will be included in the next available Chromeleon version.
Page 145 • Maintenance and Service 3. Monitor the Audit Trail of the Instrument Configuration Manager program to see whether the firmware update was successful or failed. If the firmware update failed, turn the device off and on again ¨ and repeat the firmware update. If the firmware update fails repeatedly, contact Thermo Fisher ¨...
Page 146: Replacing The Doors
• Maintenance and Service 7.12 Replacing the Doors When Damage of door TIP The maintenance procedures do not require that you remove the doors. If this should ever be required for a specific reason or procedure, follow the related steps in this section. Parts required Replacement door Follow these steps...
Page 147 • Maintenance and Service 2. Slightly tilt the door to the outside, away from the housing, and remove the door. 3. To install the door, align the door with the hinges on the housing. Be careful not to clamp tubing or capillaries between the door and the enclosure.
Page 148: Transporting Or Shipping The Device
• Maintenance and Service 7.13 Transporting or Shipping the Device If you want to transport the device to a new location or if you need to ship the device, first prepare the device for transport and then move or ship the device as required. Follow the instructions in this section. Observe the following safety guidelines: CAUTION—Heavy Load, Bulky Device The device is too heavy or bulky for one person alone to handle safely.
Page 149: Transporting The Device To A New Location
• Maintenance and Service 4. Remove the flow cell from the device and store it in its packaging. Ensure that you install the flow cell cover to the flow cell opening. Flow cells must be shipped in their original packaging. NOTICE The flow cell opening on the device is sensitive to dust and debris.
Page 150: Shipping The Device
• Maintenance and Service 4. Set up the device: a) Connect the device and set up flow connections (see Installation (} page 49)). b) Prepare the device for operation (see Preparing the Device for Operation (} page 88)). 5. Before starting an analysis, let the device equilibrate and be sure that it is ready for operation.
Page 151 • Maintenance and Service Restarting the Device after Shipping To restart the device after shipping, follow these steps: 1. Follow the unpacking instruction in this operating manual. 2. Install and set up the device in the system stack. Follow the instructions on mounting the system stack in the Vanquish System Operating Manual.
Page 152: Replacing The Slide-In Module
• Maintenance and Service 7.14 Replacing the Slide-In Module 7.14.1 Removing the Slide-In Module CAUTION—Heavy Load, Bulky Device The device is too heavy or bulky for one person alone to handle safely. To avoid personal injury or damage to the device, observe the following guidelines: •...
Page 153 • Maintenance and Service 3. Grasp the slide-in module by the cooling air intake shank and the partition panel, and pull the module out of the enclosure by approximately 10 cm. Figure 36: Pulling out the slide-in module NOTICE The slide-in module can fall down when pulling it out of the enclosure too far.
Page 154: Returning The Slide-In Module
• Maintenance and Service 7.14.2 Returning the Slide-In Module Preparation If not yet done: Remove the slide-in module from the enclosure. See Removing the Slide-In Module (} page 152). Follow these steps 1. Install the slide-in module to the enclosure of the replacement device.
Page 155 • Maintenance and Service Preparations 1. Unpack the replacement device. Remove the slide-in module from its enclosure as described in Removing the Slide-In Module (} page 152). 2. Verify that the device enclosure into which the slide-in module shall be installed is clean. If required, clean the inner and outer surfaces of the enclosure.
Page 156: Setting Up The Slide-In Module
• Maintenance and Service 4. To facilitate tightening the slide-in module in the enclosure, pull out the module a few millimeters (approximately 2 to 5 mm). 5. With the screwdriver, move each captive screw back and forth while pressing inward until the screw slips into the thread. 6.
Page 157: Troubleshooting
• Troubleshooting 8 Troubleshooting This chapter is a guide to troubleshooting issues that may arise during operation of the device. Diode Array/Multiple-Wavelength Detectors (VC-D11, VC-D12, VF-D11) Page 157 Operating Manual...
Page 158: General Information About Troubleshooting
• Troubleshooting General Information about Troubleshooting The following features help you to identify and eliminate the source for problems that may arise during operation of the device. TIP For information about operating issues that might occur during the operation of a Vanquish system, refer to the Vanquish System Operating Manual.
Page 159 • Troubleshooting Messages in the Chromeleon Audit Trail are preceded by an icon. The icon identifies the seriousness of the problem (refer to the Chromeleon Help). For possible causes and remedial actions, see Messages (} page 160). Firmware Failure If a firmware failure occurred during operation of the module, an exception log has been created about the processes during the firmware failure.
Page 160: Messages
• Troubleshooting Messages The table lists the most frequently observed messages for the device and provides troubleshooting assistance. Each message consists of a code number and a text. The code number is the unique identifier for the problem while the wording may change. Note the following: •...
Page 161 • Troubleshooting Message and Code Description and Remedial Action Code 33 Leak detected – eliminate within xx = the number of seconds within the leak must be resolved approx. xx seconds. Find and eliminate the source for the leakage (see Resolving Liquid Leaks (} page 167)).
Page 162 • Troubleshooting Message and Code Description and Remedial Action Code 3116 Data transfer error from Check the USB connection. Use only the USB cables provided by detector to PC – check USB connection. Thermo Fisher Scientific for the detector. Avoid CPU-intensive and time-consuming operations on the PC during high-speed data acquisition.
Page 163 • Troubleshooting Message and Code Description and Remedial Action Code 3132 Holmium validation failed – • Repeat the validation. wavelength outside limits. • Make sure that the lamp(s) is/are turned on, perform a wavelength calibration and repeat the wavelength validation again. •...
Page 164 • Troubleshooting Message and Code Description and Remedial Action Code 3156 xx failed. D-alpha line not xx = Wavelength calibration / wavelength validation found - check flow cell. Identification of D-alpha line during calibration/validation failed. • Repeat wavelength calibration (see Performing a Wavelength Validation and Calibration (} page 122)).
Page 165 • Troubleshooting Message and Code Description and Remedial Action Code 3206 Lamp process already in The lamp is igniting or shutting down, wait for completion. progress. Code 3207 Unexpected module behavior • Check the flow cell (see Checking the Flow Cell (} page 166)).
Page 166: Checking The Flow Cell
• Troubleshooting Checking the Flow Cell When Related messages that require a flow cell check appear in the Audit Trail. Follow these steps 1. Check that the flow cell is installed properly. 2. Remove and re-install the flow cell. 3. Flush the flow cell with pure LC/MS-grade water with a flow rate of >1 mL/min for several minutes.
Page 167: Resolving Liquid Leaks
• Troubleshooting Resolving Liquid Leaks When The leak sensor is wet. The leak sensor reports leakage. Parts and additional items required • Replacement part as required • Cloth or tissue Preparations When resolving leakage, observe the safety guidelines and general rules for maintenance and service as presented in Maintenance and Service (} page 113).
Page 168 • Troubleshooting Page 168 Diode Array/Multiple-Wavelength Detectors (VC-D11, VC-D12, VF-D11) Operating Manual...
Page 169: Specifications
• Specifications 9 Specifications This chapter provides the physical and performance specifications, including information about the materials used in the flow path of the device. Diode Array/Multiple-Wavelength Detectors (VC-D11, VC-D12, VF-D11) Page 169 Operating Manual...
Page 170: Performance Specifications
• Specifications Performance Specifications 9.1.1 Detector The device performance is specified as follows: Type Specification Optical design • Single-beam, reverse-optics design with concave holographic grating • 1024-element photodiode array Light source • Deuterium lamp for UV and VIS wavelength ranges (30 W) •...
Page 171 • Specifications Type Specification Drift <±1 mAU/h at 254 nm ASTM Drift reference conditions: • Wavelength: as listed above; bandwidth 4 nm; reference wavelength: Off; slit width Wide • Time constant: 2 s (response time = 2.2 x time constant as proposed in ASTM). For details, see below this table. •...
Page 172 • Specifications Temperature reference conditions for noise, drift and linearity specifications For drift tests, ASTM requires that temperature changes stay below 2 °C/ hour over a period of one hour. The drift specification above is based on these conditions. Larger changes in the ambient temperature will result in a larger drift.
Page 173: Flow Cells
• Specifications 9.1.2 Flow Cells The flow cell performances are as follows: Type Path length Illuminated Pressure Max. eluate Materials Bio- volume limit temperature in the flow compatibility limit path Standard flow cell, 10 mm 13 µL 12 MPa 80 °C Stainless steel, fused silica, PTFE, PEEK, titanium...
Page 174: Physical Specifications
• Specifications Physical Specifications The physical conditions of the device are specified as follows: Type Specification Range of use Indoor use only Ambient operating 5 °C - 35 °C temperature Ambient storage temperature -20 °C - 45 °C Ambient operating humidity 20% - 80% relative humidity (non-condensing) Ambient storage humidity Maximum 60% relative humidity (non-condensing) Operating altitude...
Page 175: Accessories, Consumables And Replacement Parts
• Accessories, Consumables and Replacement Parts 10 Accessories, Consumables and Replacement Parts This chapter describes the standard accessories that are shipped with the device and the accessories that are available as an option. This chapter also provides information for reordering consumables and replacement parts.
Page 176: General Information
• Accessories, Consumables and Replacement Parts 10.1 General Information The device must be operated only with the replacement parts and additional components, options, and peripherals specifically authorized and qualified by Thermo Fisher Scientific. Accessories, consumables, and replacement parts are always maintained at the latest technical standard.
Page 177: Ship Kit
• Accessories, Consumables and Replacement Parts 10.2 Ship Kit The ship kit includes the items listed in the table. The kit content is subject to change and may vary from the information in this manual. See the content list included in the kit for the most recent information about the kit content at the time when the device is shipped.
Page 178: Optional Accessories
• Accessories, Consumables and Replacement Parts 10.3 Optional Accessories Item Part No. Standard flow cell, SST, 10 mm 6083.0510 Semi-analytical flow cell, SST, 7 mm 6083.0520 Semi-micro flow cell, SST, 7 mm 6083.0530 Standard biocompatible flow cell, PEEK, 10 mm 6083.0540 Semi-micro biocompatible flow cell, MP35N, 7 mm 6083.0550 Diagnostic cell 6083.0570...
Page 179: Consumables And Replacement Parts
• Accessories, Consumables and Replacement Parts 10.4 Consumables and Replacement Parts Lamps Description Part No. UV lamp (deuterium lamp) 6083.1111 VIS lamp (tungsten lamp) 6083.2000 Capillaries for connection to the column compartment Description Part No. Viper capillary, I.D. x length 0.1 x 300 mm, MP35N 6083.7950 Viper capillary, I.D.
Page 180 • Accessories, Consumables and Replacement Parts Interface cables Description Part No. System interlink cable 6036.0004 USB cable, type A to type B, high-speed, USB 2.0 6720.8910 Cable length: 0.5 m USB cable, type A to type B, high-speed, USB 2.0 6035.9035 Cable length: 1 m USB cable, type A to type B, high-speed, USB 2.0 6911.0002...
Page 181: Appendix
• Appendix 11 Appendix This chapter provides additional information about compliance and UV cutoff wavelengths. Diode Array/Multiple-Wavelength Detectors (VC-D11, VC-D12, VF-D11) Page 181 Operating Manual...
Page 182: Compliance Information
• Appendix 11.1 Compliance Information 11.1.1 Declarations of Conformity CE Declaration of Conformity The device has satisfied the requirements for the CE mark and is compliant with the applicable requirements. cTUVus Compliance The cTUVus label on the device indicates that the device has satisfied the requirements for the cTUVus mark.
Page 183: Weee Compliance
• Appendix 11.1.2 WEEE Compliance This product is required to comply with the European Union’s Waste Electrical & Electronic Equipment (WEEE) Directive. It is marked with the following symbol: Figure 38: WEEE symbol Thermo Fisher Scientific has contracted with one or more recycling or disposal companies in each European Union (EU) Member State, and these companies should dispose of or recycle this product.
Page 184: Manual Release History
• Appendix 11.1.5 Manual Release History Revision Covering VC‑D11-A-01; VC‑D12-A-01; VF‑D11‑A‑01 VF‑D11‑A‑01 The instructions were prepared in English (original instructions). Other language versions are translations based on the English original instructions. Page 184 Diode Array/Multiple-Wavelength Detectors (VC-D11, VC-D12, VF-D11) Operating Manual...
Page 185: Uv Cutoff Wavelengths Of Solvents
• Appendix 11.2 UV Cutoff Wavelengths of Solvents The UV cutoff wavelength is the minimum effective wavelength for the measurement. The mobile phase composition affects its UV cutoff wavelength. In general, mobile phases are solvents, such as, water, acetonitrile, methanol, or other substances. They may also contain salts, such as sodium hydroxide (NaOH).
Page 186 • Appendix Page 186 Diode Array/Multiple-Wavelength Detectors (VC-D11, VC-D12, VF-D11) Operating Manual...
Page 187: Index
Index Index Numerical Smart Standby........... 108 Smart Startup .......... 89 3D field ............. 97 cleaning ............ 119 condensation.......... 54, 58 connectors............ 57 accessories .......... 47, 175 consumables .......... 175, 179 optional ............. 178 cTUVus mark .......... 182 ship kit............ 177 acquisition off........... 95 acquisition on ........... 95 data collection rate ...... 96, 100, 101 additives ............ 27 guidelines .......... 101 information .......... 27...
Page 188 Index exchange ........... 131, 134 flushing............ 132 keypad.............. 84 guidelines ............ 90 UV.............. 85 identification chip ........ 37 VIS ............... 85 initial installation......... 62 remove ............ 134 safety........... 90, 131 lamp ............... 124 shipping............. 131 UV.............. 95 storage ............ 131 VIS ............... 95 types............ 38 lamp replacement .......... 124 waste line replacement..... 140, 141 deuterium lamp (UV) ........ 124 fuses ............... 142 tungsten lamp (VIS)........ 128 lamps .............. 39...
Page 189 Index replacement parts ........ 175, 179 response time........ 97, 100, 102 NIST compliance.......... 183 optimization .......... 102 null balancing ........... 98 restart device after long-term shutdown.. 112 RoHS mark............ 182 operating principle ........... 34 operation............ 41, 81 safety class ............ 21 control elements ......... 84 safety glasses............ 23 flow cells ............. 90 safety guidelines important parameters......... 95 case of emergency ........ 26...
Page 190 Index slit width........... 96, 100, 104 effect on bandwidth........ 102 Viper fitting system .......... 73 Smart Shutdown.......... 108 VIS button............ 85 Smart Standby .......... 108 VIS lamp ............ 95 Smart Startup ........... 89 turn off ............ 93 solvent turn on ............ 93 chloride concentration........ 28 information .......... 27 pH range............ 28 specifications.......... 169 waste line ............ 76 performance (detector) ...... 170 wavelength.......... 96, 100...
Page 191 Index Diode Array/Multiple-Wavelength Detectors (VC-D11, VC-D12, VF-D11) Page 191 Operating Manual...
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ThermoFisher Scientific Vanquish VC-D11 Operating Manual

1 Using this Manual

2 Safety

3 Device Overview

4 Unpacking

5 Installation

6 Operation

7 Maintenance and Service

8 Troubleshooting

9 Specifications

10 Accessories, Consumables and Replacement Parts

11 Appendix

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