Page 1 thermoscientific Vanquish Diode Array Detectors VH-D10 Operating Manual 4820.8301‐EN Revision 2.0a • September 2017 ...
Page 2 Copyright © 2017 Thermo Fisher Scientific Inc. All rights reserved. Original Operating Manual The hardware descriptions in this manual revision refer to detectors VH-D10. For the manual release history, see page 188. Trademarks Torx is a trademark of TEXTRON INDUSTRIES, Incorporated. Acrobat, Adobe, and Adobe Reader are trademarks of Adobe Systems Incorporated.
Page 3 Contacting Us Contacting Us There are several ways to contact us: Ordering Information For ordering information or sales support for HPLC products, contact your local Thermo Fisher Scientific sales organization. For contact information, go to Contact Us on http://www.thermofisher.com. Technical Assistance For technical support for HPLC products, contact your local Thermo Fisher Scientific support organization.
Page 4 Contacting Us Page 4 Vanquish Diode Array Detectors (VH-D10) Operating Manual...
Page 5: Table Of Contents
Contents Contents 1 Using this Manual ................9 About this Manual ....................10 Conventions ......................11 1.2.1 Safety Messages ..................11 1.2.2 Special Notices and Informational Notes ..........11 1.2.3 Typographical Conventions ..............12 Reference Documentation ..................13 2 Safety ....................15 Safety Symbols and Signal Words .................
Page 6 Contents 4 Unpacking ..................39 Unpacking ......................40 Scope of Delivery ....................43 5 Installation ..................45 Safety Guidelines for Installation ................. 46 Installing the Detector ..................47 Site Requirements ....................49 Accessing the Interior Components ..............51 Setting Up the Hardware ..................52 5.5.1 System Arrangement................
Page 7 Contents Important Operating Parameters ................. 94 Optimizing the Performance of the Detector ............. 100 6.7.1 General Guidelines ................. 100 6.7.2 Overview of Optimization Parameters ..........101 6.7.3 Selecting the Data Collection Rate ............102 6.7.4 Selecting the Response Time and Peak Width ........102 6.7.5 Optimizing the Bandwidth and Slit Width Settings ........
Page 8 Contents 7.14 Transporting or Shipping the Detector .............. 152 7.14.1 Preparing the Detector for Transport ........... 153 7.14.2 Transporting the Detector to a New Location ........154 7.14.3 Shipping the Detector ................155 7.15 Slide-In Module ....................157 7.15.1 Removing the Slide-In Module .............. 157 7.15.2 Installing the Slide-In Module ...............
Page 9: Using This Manual
• Using this Manual 1 Using this Manual This chapter provides information about this manual, the conventions used throughout the manual, and the reference documentation that is available in addition to this manual. Vanquish Diode Array Detectors (VH-D10) Operating Manual Page 9...
Page 10: About This Manual
• Using this Manual About this Manual This manual describes the functional features and operating principle of your Vanquish™ detector and provides instructions for installation, set up, start up, shut down, operation, maintenance and troubleshooting. The layout of this manual is designed to provide quick reference to the sections of interest to the user.
Page 11: Conventions
• Using this Manual Conventions This section describes the conventions that are used throughout this manual. 1.2.1 Safety Messages The safety messages and precautionary statements in this manual appear as follows: • Safety messages or precautionary statements that apply to the entire manual and all procedures in this manual are grouped in the Safety chapter.
Page 12: Typographical Conventions
• Using this Manual 1.2.3 Typographical Conventions These typographical conventions apply to the descriptions in this manual: Data Input and Output • The following appears in bold type: ♦ Input that you enter by the keyboard or that you select with the mouse ♦...
Page 13: Reference Documentation
• Using this Manual Reference Documentation In addition to this operating manual, other documentation is available for reference. Hardware Documentation Additional hardware documentation includes the following: • Operating manuals for the other modules of the Vanquish system A printed version of the manual is shipped with the device. •...
Page 14 • Using this Manual TIP The Chromeleon Help and documents are included in the software shipment. Third-Party Documentation Refer also to the user documentation provided by the manufacturers of third-party components and materials, for example, Safety Data Sheets (SDSs). Page 14 Vanquish Diode Array Detectors (VH-D10) Operating Manual...
Page 15: Safety
• Safety 2 Safety This chapter provides general and specific safety information and informs about the intended use of the detector. Vanquish Diode Array Detectors (VH-D10) Operating Manual Page 15...
Page 16: Safety Symbols And Signal Words
• Safety Safety Symbols and Signal Words 2.1.1 Safety Symbols and Signal Words in This Manual This manual contains safety messages to prevent injury of the persons using the detector. The safety symbols and signal words in this manual include the following: Always be aware of the safety information.
Page 17: Observing This Manual
• Safety 2.1.2 Observing this Manual Observe the following: • Before installing or operating the detector, read this manual carefully to be familiar with the detector and this manual. The manual contains important information with regard to user safety as well as use and care of the detector.
Page 18: Rating Plate
• Safety 2.1.4 Rating Plate The rating plate is present on the detector near the electrical connections. The rating plate indicates the serial number, part number, module name, revision number (if any), line and fuse rating, and the manufacturer's address. TIP An additional type label on the leak tray of the detector indicates the module name, serial number, part number, and revision number (if any).
Page 19: Intended Use
• Safety Intended Use The detector is intended to be part of the Vanquish system. The intended use of the Vanquish system is to analyze mixtures of compounds in sample solutions. The detector is for use by qualified personnel and in laboratory environment only. The detector and Vanquish system are intended for laboratory research use only.
Page 20: Safety Precautions
• Safety Safety Precautions 2.3.1 General Safety Information All users must observe the general safety information presented in this section and all specific safety messages and precautionary statements elsewhere in this manual during all phases of installation, operation, troubleshooting, maintenance, shutdown, and transport of the detector. If the detector is used in a manner not specified by Thermo Fisher Scientific, the protection provided by the detector could be impaired.
Page 21: Qualification Of The Personnel
• Safety 2.3.2 Qualification of the Personnel Observe the information below on the proper qualification of the personnel installing and/or operating the detector. Installation Only skilled personnel are permitted to install the detector and to establish the electrical connections according to the appropriate regulations.
Page 22: Electrical Safety Precautions
• Safety Protective Eyewear To prevent liquids from striking your eyes, put on appropriate protective eyewear, such as safety glasses with side shields. If there is a risk of splashing liquids, put on goggles. Gloves To protect you from harmful liquids and avoid personal injury during maintenance or service, put on appropriate protective gloves.
Page 23: General Residual Hazards
• Safety 2.3.5 General Residual Hazards Pay attention to the following general residual hazards when working with the detector: WARNING—Hazardous Substances Solvents, mobile phases, samples, and reagents might contain toxic, carcinogenic, mutagenic, infectious, or otherwise harmful substances. The handling of these substances can pose health and safety risks. •...
Page 24 • Safety WARNING—Biohazard Biohazardous material, for example microorganisms, cell cultures, tissues, body fluids, and other biological agents can transmit infectious diseases. To avoid infections with these agents: • Assume that all biological substances are at least potentially infectious. • Wear personal protective equipment as required by the hazard and follow good laboratory practice.
Page 25: In Case Of Emergency
• Safety CAUTION—Escape of Hazardous Substances from PEEK Capillaries In the Vanquish system, capillaries made of PEEK may be used. Swelling or attack by acids can cause PEEK capillaries to start leaking or to burst. • Certain chemicals, for example, trichlormethane (CHCl ), dimethyl sulfoxide (DMSO), or tetrahydrofuran (THF) can cause PEEK to swell.
Page 26: Solvent And Additive Information
• Safety Solvent and Additive Information To protect optimal functionality of the Vanquish system, observe these recommendations on the use of solvents and additives: • The system must be used with reversed-phase (RP) compatible solvents and additives only. • Use only solvents and additives that are compatible with all parts in the flow path.
Page 27 • Safety NOTICE If the system flow path includes a Vanquish charged aerosol detector, observe the specific solvent and additive information for this detector. Refer to the operating manual for the Vanquish charged aerosol detector. • Follow any specific recommendations presented in other sections of this manual.
Page 28: Compliance Information
• Safety Compliance Information Thermo Fisher Scientific performs complete testing and evaluation of its products to ensure full compliance with applicable domestic and international regulations. When the device is delivered to you, it meets all pertinent electromagnetic compatibility (EMC) and safety standards as described in this manual.
Page 29: Detector Overview
• Detector Overview 3 Detector Overview This chapter introduces you to the detector and the main components. Vanquish Diode Array Detectors (VH-D10) Operating Manual Page 29...
Page 30: Detector Features
• Detector Overview Detector Features The detector comprises the following main features: • A deuterium lamp for ultraviolet (UV) and visible (VIS) wavelength detection as the light source of the detector The deuterium lamp provides light for the complete wavelength detection range from 190 nm to 680 nm.
Page 31: Operating Principle
• Detector Overview Operating Principle The detector is designed for ultraviolet (UV) and visible (VIS) absorption spectroscopy in combination with HPLC or UHPLC separations. After sufficient separation from other sample compounds, the analysis of the target compound follows the Beer-Lambert law. This means that the response of the detector is proportional to the concentration of the analyte.
Page 32 • Detector Overview A deuterium lamp (1) as light source emits light in the UV and VIS spectral range. The lamp mirror (2) focuses the light to the entrance of the flow cell (4). The shutter (motorized filter paddle, 3) can be opened in the light path before the flow cell.
Page 33: Interior Components
• Detector Overview Interior Components The user-accessible components of the detector are located directly behind the front doors: Figure 2: Interior view (here with flow cell installed) Description Cooling air intake Capillary guide slit below the cooling air intake To hold the capillary if the column compartment is located to the left of the detector.
Page 34: Flow Cell
• Detector Overview Flow Cell The detector design allows for easy access to the flow cell on the interior front. Figure 3: LightPipe flow cell (here: with plugs installed on inlet and outlet) Flow Cell Label On the front of the flow cell, a flow cell label is present, which contains information about the flow cell, such as flow cell type, part number, and serial number.
Page 35 • Detector Overview The flow cells are optimized for highest possible light transmission and transmission stability for the complete wavelength range of the detector from 190 nm to 680 nm. The following flow cells are available for the detector: • LightPipe flow cell, standard, path length 10 mm •...
Page 36: Lamp
• Detector Overview Lamp As light source for ultraviolet (UV) and visible (VIS) wavelength detection, the deuterium lamp provides light for the complete wavelength detection range from 190 nm to 680 nm. The lamp is equipped with an identification (ID) chip. The ID chip stores information about the lamp, including the number of lamp ignitions and the operating time of the lamp, thus, providing an overview of the lamp status.
Page 37: Leak Detection
• Detector Overview Leak Detection Leaks are a potential safety issue. The leak sensor inside the detector monitors the detector for liquid leaks from the flow connections. The liquid is collected in the leak tray and guided to the drain port. From the drain port, the liquid is discharged to waste through the drain system of the Vanquish system.
Page 38: Operation
• Detector Overview Operation The detector is designed to be operated from a computer configured with the Chromeleon Chromatography Data System (CDS). The Chromeleon software provides complete instrument control, data acquisition, and data management. For a basic description of instrument control and automated sample analysis with the Chromeleon software, refer to the Vanquish System Operating Manual.
Page 39: Unpacking
• Unpacking 4 Unpacking This chapter provides information for unpacking the detector and informs you about the scope of delivery. Vanquish Diode Array Detectors (VH-D10) Operating Manual Page 39...
Page 40: Unpacking
• Unpacking Unpacking Damaged Packaging, Defective on Arrival Inspect the shipping container for signs of external damage and, after unpacking, inspect the detector for any signs of mechanical damage that might have occurred during shipment. If you suspect that the detector may have been damaged during shipment, immediately notify the incoming carrier and Thermo Fisher Scientific about the damage.
Page 41 • Unpacking 3. Remove the detector from the shipping container: Grasp the detector by the carrying handles. Slowly and carefully, lift the detector out of the shipping container. Figure 5: Carrying handles on the detector Component Carrying handles Attachment screw (one on each carrying handle) 4.
Page 42 • Unpacking TIP Keep the shipping container, the carrying handles with the attachment screws, and all packing material. These items will be needed if the detector is transported to a new location or shipped. 9. Some surfaces including the doors of the detector are covered by a protective film during shipment.
Page 43: Scope Of Delivery
• Unpacking Scope of Delivery The following items are included in the delivery: • Detector • Ship Kit For details about the kit content, see section 10.2 Ship Kit, page 181. • Operating manual • Power cord Vanquish Diode Array Detectors (VH-D10) Operating Manual Page 43...
Page 44 • Unpacking Page 44 Vanquish Diode Array Detectors (VH-D10) Operating Manual...
Page 45: Installation
• Installation 5 Installation This chapter specifies the requirements for the installation site and describes how to set up, install, and configure the detector in the Vanquish system and in the chromatography software. Vanquish Diode Array Detectors (VH-D10) Operating Manual Page 45...
Page 46: Safety Guidelines For Installation
• Installation Safety Guidelines for Installation Pay attention to the following safety guidelines: Observe all warning messages and precautionary statements presented section 2.3 Safety Precautions (see page 20). CAUTION—Heavy Load, Bulky Device The detector is too heavy or bulky for one person alone to handle safely. To avoid personal injury or damage to the detector, observe the following guidelines: •...
Page 47: Installing The Detector
• Installation Installing the Detector The Vanquish system is installed and set up by a Thermo Fisher Scientific service engineer, including all modules and options or parts shipped with them. The service engineer checks that the installation is correct and that the Vanquish system and modules operate as specified.
Page 48 • Installation 6. Recommended: Perform Instrument Installation Qualification. In Chromeleon, a wizard is available to guide you through the qualification process: ♦ On the Chromeleon 7 Console: Click Tools > Instrument Qualification > Installation Qualification. ♦ In the Chromeleon 6.8 Browser: Click Qualification > Instruments IQ.
Page 49: Site Requirements
• Installation Site Requirements The operating environment is important to ensure optimal performance of the detector. This section provides important requirements for the installation site. Note the following: • Operate the detector only under appropriate laboratory conditions. • The detector is intended to be part of the Vanquish system. Observe the site requirements for the Vanquish system as stated in the Vanquish System Operating Manual.
Page 50 • Installation Power Cord The power cords are designed to match the wall socket requirements of the country in which they are used. The end of the power cords that plugs into the power socket on the device is identical for all power cords. The end of the power cords that plugs into the wall socket is different.
Page 51: Accessing The Interior Components
• Installation Accessing the Interior Components To access the interior components in the detector, open the front doors. To allow easy access from the front, the user-accessible components and flow connections in the detector are located directly behind the doors. Figure 7: Opening the front doors Vanquish Diode Array Detectors (VH-D10) Operating Manual Page 51...
Page 52: Setting Up The Hardware
• Installation Setting Up the Hardware This section describes how to set up the hardware and provides information about the detector connectors and cables. 5.5.1 System Arrangement The detector is part of the Vanquish system. The system modules are typically arranged in a system stack, with the arrangement depending on the system configuration.
Page 53 • Installation Description Solvent Rack Detector Autosampler Pump System Base Column Compartment For instructions on how to set up the system stack, refer to the Vanquish System Operating Manual. Vanquish Diode Array Detectors (VH-D10) Operating Manual Page 53...
Page 54: Connecting The Detector
• Installation 5.5.2 Connecting the Detector Detector Connectors The following connectors are provided on the detector: Figure 9: Electrical connectors on the right side of the detector Description Rating plate, indicating the serial number, part number, module name, revision number (if any), line and fuse rating, and the manufacturer's address Main power switch (on/off control) Fuse holder Power-inlet connector...
Page 55 • Installation Description USB hub ("A" type connector) Allows connection to other modules in the Vanquish system USB (Universal Serial Bus) port ("B" type connector) Allows connection to other modules in the Vanquish system or the computer on which the data management system is installed, such as the Chromeleon software TIP Thermo Fisher Scientific recommends using the USB ports only as described above.
Page 56 • Installation 4. Connect the power cord to the power-inlet connector on the detector. NOTICE Before connecting the detector to the power line, be sure that no condensation is present in the device. Condensation in the device can damage the electronics and optics. If you suspect that condensation is present, allow the device to warm up to room temperature.
Page 57: Installing The Flow Cell
• Installation 5.5.3 Installing the Flow Cell This section describes the installation of the LightPipe flow cell upon initial installation of the detector. For instructions on exchanging a flow cell or installing a flow cell after storage, see section 7.7, page 126.
Page 58 • Installation Parts required LightPipe flow cell Figure 10: Front side of the flow cell Description Outlet (OUT) (closed with plug during storage and transport) Inlet (IN) (closed with plug during storage and transport) Flow cell label Rotating locks (unlocked position) Page 58 Vanquish Diode Array Detectors (VH-D10) Operating Manual...
Page 59 • Installation Preparations 1. On the interior front of the detector, turn the rotating locks on the cover of the flow cell opening counterclockwise until they are in a horizontal position. 2. Remove the cover from the flow cell opening. Keep the cover to close the flow cell opening when no flow cell is installed in the detector.
Page 60 • Installation Follow these steps 1. Remove the shipping locks carefully on the left and right rear side of the flow cell. TIP Store the shipping locks in the cell packaging to have them easily available when storing or shipping the cell. Figure 12: Rear side of the flow cell Description Identification chip...
Page 61 • Installation 3. Carefully insert the flow cell into the flow cell opening in the detector. The flow cell must sit completely in the opening. Figure 13: Inserting the flow cell with opened rotating locks 4. Turn the rotating locks simultaneously clockwise until they are in a vertical position.
Page 62: Setting Up The Flow Connections
• Installation Setting Up the Flow Connections 5.6.1 General Information and Guidelines When setting up flow connections, follow these rules and recommendations: Flow connections can be filled with hazardous substances. Observe the warning messages and precautionary statements presented in section 2.3 Safety Precautions (see page 20).
Page 63: Guiding Capillaries And Tubing Through The System
• Installation 5.6.2 Guiding Capillaries and Tubing Through the System Flow connections between the modules of the Vanquish system are guided through either the tubing chase in the devices or the guide holes or capillary clips of the devices. Tubing Chase, Tubing Guide, Tubing Bracket To guide certain tubes and lines (solvent tubing, wash liquid tubing, detector waste line) from the top module to the bottom module in the Vanquish system stack, the stackable modules have a tubing chase on...
Page 64: Connecting Fittings, Capillaries, And Tubing
• Installation Tubing brackets are available for holding the tubing in place. Slip the bracket side onto the drain pipe. Figure 16: Tubing bracket (left), tubing bracket installed (right) Guide Holes and Capillary Clips Guide holes and capillary clips are provided at specific positions on the system modules.
Page 65 • Installation Connecting Viper Capillaries 5.6.3.2 This section describes how to connect Viper™ capillaries. All Viper flow connections in the Vanquish system are designed to be finger-tight. To connect Viper capillaries with knurl, follow these steps: NOTICE • Tighten or loosen Viper capillaries only with your fingers. Do not use tools other than the knurl that comes with the capillary.
Page 66 • Installation c) Clean the capillary ends carefully by using a lint-free tissue wetted with isopropanol. d) Reinstall the capillary. e) If the connection continues to leak, install a new Viper capillary. Page 66 Vanquish Diode Array Detectors (VH-D10) Operating Manual...
Page 67: Flow Connections To The Flow Cell
• Installation 5.6.4 Flow Connections to the Flow Cell Set up the flow connections to the flow cell when the flow cell is installed to the detector. NOTICE Flow cells are highly sensitive to contamination, clogging and high backpressures. Even if the pressure exceeds the upper limit for a very short time only, the flow cell may be permanently damaged.
Page 68 • Installation Parts required • Inlet capillary for connection of the column in the Vanquish column compartment to the flow cell in the detector • Plugs for the partition panel from the detector ship kit (if not pre- installed), depending on the capillary to be installed: ♦...
Page 69 • Installation 3. On the detector partition panel, make sure that you use suitable plugs in the panel depending on the capillaries to be installed: ♦ For capillaries with small outer diameter, use the plug with slit. Observe the following: ♦...
Page 70 • Installation Follow these steps 1. Connect the inlet of the flow cell: Connect the capillary from the column to the flow cell. See section 5.6.4.1, page 2. Connect the outlet of the flow cell: ♦ If the detector is the last module in the system flow path, connect the detector waste line to the flow cell.
Page 71 • Installation 3. Connect the capillary to the flow cell inlet. Figure 21: Capillaries properly connected in the detector Connecting the Detector Waste Line 5.6.4.2 Preparations 1. See section 5.6.4 Flow Connections to the Flow Cell, page 2. On the system base, route the detector waste line through the dedicated detector waste outlet in the base to waste.
Page 72: Guiding Liquid Leaks To Waste
• Installation 4. Check the waste line over the entire flow path: a) Make sure that no bending (kink), pinching or squeezing of the waste line is present at any point in the flow path. b) If you have to cut tubing to length, use a tubing cutter. Make sure that the cut is at right angle to the length of the line.
Page 73: Determining The Backpressure In The Flow Cell
• Installation Determining the Backpressure in the Flow Cell All modules, capillaries and waste lines downstream of the flow cell contribute to the backpressure on the flow cell. If you attach other modules (such as detectors or a mass spectrometer) in the flow path after the flow cell, make sure that the pressure within the flow cell does not exceed 6 MPa.
Page 74: Determining The Backpressure Without Lightpipe Flow Cell
• Installation Follow these steps 1. Determine the backpressure in the system configuration with mass spectrometer/second detector but without installed LightPipe flow cell. See section 5.7.1, page 2. Determine the backpressure of the LightPipe flow cell. See section 5.7.2, page 5.7.1 Determining the Backpressure without LightPipe flow cell NOTICE Do not install switching valves or fraction collectors downstream...
Page 75 • Installation 7. Read the system pressure in Chromeleon and write down the value for P1. 8. Stop the pump flow. 9. Disconnect the waste line from the Viper union. WARNING—Escape of Hazardous Substances from Flow Connections Flow and capillary connections can be filled with substances that can pose health risks.
Page 76: Determining The Backpressure Of The Lightpipe Flow Cell
• Installation 16. Calculate the difference between the two measured pressure values: P3 = P2 – P1. ♦ If P3 exceeds 6 MPa: ♦ Reduce the flow rate and restart the procedure with step –or– ♦ Do not use this system configuration, as the backpressure will destroy the flow cell.
Page 77 • Installation 7. Calculate the sum of the pressure values P6 = P5 + P3: If P6 is below 6 MPa: a) Increase the pump flow rate in steps of 0.5 mL/min b) Read the system pressure in Chromeleon c) Write down the values and repeat steps 3 through until P6 is...
Page 78: Turning On The Detector
• Installation Turning On the Detector TIP Before turning on the power to a Vanquish system module for the first time, verify that the chromatography software is installed on the data system computer. When the power is turned on, the required USB drivers are automatically found and the Windows™...
Page 79: Setting Up The Detector In The Software
• Installation Setting Up the Detector in the Software This manual assumes that the chromatography software is already installed on the data system computer and a valid license is available. For more information about setting up the Vanquish system in the software, refer to the Vanquish System Operating Manual.
Page 80 • Installation Page 80 Vanquish Diode Array Detectors (VH-D10) Operating Manual...
Page 81: Operation
• Operation 6 Operation This chapter describes the elements for detector control, provides information for routine operation and for shutdown. Vanquish Diode Array Detectors (VH-D10) Operating Manual Page 81...
Page 82: Introduction To This Chapter
• Operation Introduction to this Chapter The information in this chapter assumes that the initial setup of the detector has already been completed. If this is not the case, refer to the instructions in chapter 5 Installation before proceeding (see page 45).
Page 83: Safety Guidelines For Operation
• Operation Safety Guidelines for Operation When operating the detector, pay attention to the following safety guidelines: Observe all warning messages and precautionary statements presented section 2.3 Safety Precautions (see page 20). CAUTION—Hot Surfaces Surfaces inside the detector may become hot during operation. Touching hot parts might cause burns.
Page 84: Control Elements
• Operation Control Elements The detector is designed to be operated mainly from a computer running with the chromatography software. In addition, the following elements are available on the detector: • Keypad The keypad buttons allow you to perform certain functions directly from the detector.
Page 85 • Operation MUTE ALARM If beeping alerts you that the detector firmware detected a problem, for example leakage, beeping continues until you turn off the beep. Pressing this button turns off the beep for the current alarm. As a standard, beeping starts again after 10 minutes if the problem is still present or if the firmware detected another problem.
Page 86: Status Indicators
• Operation 6.3.2 Status Indicators The status LED bar on the front side of the detector and the STATUS LED on the inside keypad provide information about the detector status. LED Bar The LED bar provides the information when the detector is closed. When the detector is connected in the Chromeleon software, the LED bar may provide less information.
Page 87 • Operation STATUS LED The STATUS LED on the keypad inside the detector provides the following information: STATUS LED Description Off (dark) The power to the detector is turned off. Green The detector is functioning properly. A problem or error has occurred. For the related message, check the Chromeleon Audit Trail.
Page 88: Power On/Off Control
• Operation Power On/Off Control The power switch on the detector is the main switch for power on/off control. The main power switch is turned on during initial installation of the detector. For easier handling, you can use the power button on the front left of the Vanquish system base (system power button) for power on/off.
Page 89: Preparing The Detector For Operation
• Operation Preparing the Detector for Operation This section gives information on any additional steps that are required to prepare the detector for operation and sample analysis. Before Operating the Detector for the First Time Prepare the detector for the first-time operation, observing the following: NOTICE Flush the system flow path thoroughly before operating the detector for the first time:...
Page 90 • Operation Before Starting Sample Analysis Before starting an analysis: • Check the liquid level in the solvent reservoirs. Verify that the amount of solvent is sufficient for the analysis. • Verify that the doors of the modules in the Vanquish system are closed.
Page 91: Guidelines For Use Of Lightpipe Flow Cells
• Operation TIP The Chromeleon software supports procedures for automatically starting a chromatography system in the software (Smart Startup). The startup procedure includes the operations for system equilibration. For details, refer to the Chromeleon Help. TIP When you start the data acquisition or perform an autozero, the shutter in the light path before the flow cell is opened automatically.
Page 92 • Operation • Solvents with a low boiling point can evaporate in the warm flow cell, thus degrading the performance of the flow cell. Consider that the combination of temperature and pressure of the eluate in the flow cell can prevent boiling of the eluate. •...
Page 93: Turning On The Lamp
• Operation 6.5.2 Turning On the Lamp You can turn on the lamp using the UV button on the keypad of the detector or using Chromeleon. Allow the lamp to warm up and stabilize for at least one hour until the working temperature is achieved before you start analysis.
Page 94: Important Operating Parameters
• Operation Important Operating Parameters The parameters described in this section should be considered for routine operation of the detector. You can usually access these parameters from the Chromeleon user interface. If a parameter listed below is not available in Chromeleon, consider updating the firmware and Chromeleon version.
Page 95 • Operation Parameters Description Set a wavelength for each UV_VIS_x signal channel Wavelength individually, in the range of 190 nm to 680 nm for up to 10 signal channels. The wavelength defines the value at which the detector measures the absorbance of the analyte(s) in the sample. Observe the following: •...
Page 96 • Operation Parameters Description Slit width The slit width determines how much light passes through the adjustable entrance slit and is available for measurement. As more light is available for the measurement, the baseline noise is minimized. However, the optical resolution diminishes. The setting of the slit width affects all signal channels and the 3D field.
Page 97 • Operation Parameters Description Reference wavelength A reference wavelength can be selected and set optionally to correct the signal measured from the sample if interfering substances absorb in addition to the absorption of the analyte. As a standard, no reference wavelength is set. To use the Reference Wavelength setting, select it separately for each signal channel.
Page 98 • Operation Parameters Description Autozero Perform an automatic null balancing. The current detector signal is interpreted as 0. Therefore, no absorbing sample should be in the flow cell when Autozero is performed. TIP When you start the data acquisition or perform an autozero, the shutter in the light path before the flow cell is opened automatically.
Page 99 • Operation Parameters Description Analog out board For configuring the analog out connections, parameters parameters are available for Analog Out port 1 or 2, respectively. Analog1_Offset Adjusts the zero position of the analog output when it is plotted. The value entered is a percentage of the full-scale Analog2_Offset analog output.
Page 100: Optimizing The Performance Of The Detector
• Operation Optimizing the Performance of the Detector This section provides information for best performance of the detector and gives hints on what you can do to optimize the performance further. For information on the default parameters, see section 6.6 Important Operating Parameters, page 6.7.1 General Guidelines...
Page 101: Overview Of Optimization Parameters
• Operation • Degas the solvent. • Monitor the usage of specific detector components that are subject to wear and stress and schedule appropriate maintenance intervals (see section 7.4.3 Predictive Performance, page 120). • For information about the UV cutoff wavelengths of solvents, see section 11.2 UV Cutoff Wavelengths of Solvents, page 189.
Page 102: Selecting The Data Collection Rate
• Operation 6.7.3 Selecting the Data Collection Rate The data collection rate is the number of data points per second (Hz) that the Chromeleon software collects from the detector and stores as raw data. When you select a data collection rate, observe the following guidelines: •...
Page 103: Optimizing The Bandwidth And Slit Width Settings
• Operation 6.7.5 Optimizing the Bandwidth and Slit Width Settings As a standard, a bandwidth of 4 nm and a slit width of 4 nm are defined in Chromeleon. If required, both parameters can be set individually. The interaction between bandwidth and slit width has a substantial impact on linearity, baseline noise, selectivity as well as the spectral resolution of the peak.
Page 104: Selecting The Reference Wavelength And Bandwidth
• Operation Slit Width A narrow slit width results in a smaller optical bandwidth and provides a better optical resolution (the ability of the detector to distinguish between single wavelengths) which is required for analytes with fine spectral structures (such as benzene). Set a value for the slit width that it is not smaller than the smallest value of all bandwidths and bunch widths used for measurement.
Page 105 • Operation Guidelines To reduce the interference effectively, select the reference wavelength so that it meets the following requirements: • The absorption of the interfering substance is approximately the same at the measurement wavelength and the reference wavelength. If the height of the interfering absorption differs between the measurement wavelength and the reference wavelength, the interference is insufficiently or too much compensated.
Page 106: Shutting Down The Detector
• Operation Shutting Down the Detector If the detector will not be operated for some time, follow the instructions on shutting down the detector in this section. TIP The Chromeleon software provides procedures for automatically preparing the chromatography system for shutdown. The procedures include, for example, operations for reducing the flow rate, reducing the temperature in temperature-controlled devices, and turning off the detector lamps.
Page 107: Long-Term Shutdown
• Operation • For LightPipe flow cells, note the following: ♦ If pump flow is interrupted and the lamp is turned on, protect the flow cell from the light of the lamp: Close the shutter in the light path before the flow cell, or turn off the lamp. ♦...
Page 108 • Operation Observe the following: ♦ Detector and flow cell remain in the laboratory after shutdown ♦ If no additive is used, flush the system, for example with methanol. 100% acetonitrile should not be used. ♦ If an additive is used, flush the system with several volumes (for example, 1.0 mL/min for 10 minutes with the standard system) of methanol and water (50:50) to prevent salt buildup in the fluidics.
Page 109 • Operation 6. The step depends as follows: ♦ Detector and all other system modules remain in the system stack and are to be turned off Turn off the system with the system power button on the system base. ♦ Detector shall be transported or shipped after shutdown If one of the modules shall be removed from the system stack, turn off all system modules with their main power switch.
Page 110 • Operation Page 110 Vanquish Diode Array Detectors (VH-D10) Operating Manual...
Page 111: Maintenance And Service
• Maintenance and Service 7 Maintenance and Service This chapter describes the routine maintenance and the service procedures that the user may perform. Vanquish Diode Array Detectors (VH-D10) Operating Manual Page 111...
Page 112: Introduction To Maintenance And Service
• Maintenance and Service Introduction to Maintenance and Service This chapter describes the routine maintenance and service and repair procedures that the user may perform. Additional maintenance or service procedures must be performed only by service personnel certified by Thermo Fisher Scientific (for brevity, referred to as Thermo Fisher Scientific service personnel).
Page 113: Safety Guidelines For Maintenance And Service
• Maintenance and Service Safety Guidelines for Maintenance and Service 7.2.1 General When performing maintenance or service procedures, pay attention to the following safety guidelines: Observe all warning messages and precautionary statements presented section 2.3 Safety Precautions, page WARNING—High Voltage High voltages are present inside the detector that could cause an electric shock.
Page 114 • Maintenance and Service CAUTION—Spraying Solvent Solvents can spray when under high pressure. • Stop the pump flow prior to opening the flow path. • Wait until the system pressure is down to zero. • When opening the flow path, wear appropriate protective equipment.
Page 115: Flow Cells
• Maintenance and Service 7.2.2 Flow Cells NOTICE—Sensitive LightPipe Flow Cells LightPipe flow cells are highly sensitive. Improper use or handling can lead to increased noise, increased drift, increased refractive index sensitivity, clogging, leaks on the flow cell, or even destruction of the flow cell.
Page 116: General Rules For Maintenance And Service
• Maintenance and Service General Rules for Maintenance and Service For successful maintenance and service procedures, follow these rules and recommendations: • Before starting maintenance or service procedures, shut down the detector when instructed to do so. • Use only the replacement parts specifically authorized and qualified for the detector by Thermo Fisher Scientific.
Page 117: Routine And Preventive Maintenance
• Maintenance and Service Routine and Preventive Maintenance Optimum detector performance, maximum uptime of the detector, and accurate results can be obtained only if the detector is in good condition and properly maintained. 7.4.1 Maintenance Plan Perform the maintenance procedures in the table on a regular basis. The frequency given in the table is a suggestion.
Page 118: Cleaning Or Decontaminating The Detector
• Maintenance and Service 7.4.2 Cleaning or Decontaminating the Detector Cleaning and decontamination must be performed by qualified personnel wearing suitable personal protective equipment. Always observe national and local regulations. NOTICE Wipe up all liquids spilled onto the system immediately. If surfaces are exposed for longer periods, these liquids can cause damage.
Page 119 • Maintenance and Service NOTICE Observe the following: • Only use cleaning detergents that will not damage the surfaces of the system. • Never use sharp tools or brushes for cleaning any surfaces. • Do not use sprays for cleaning. •...
Page 120: Predictive Performance
• Maintenance and Service 7.4.3 Predictive Performance The Chromeleon software supports functions for estimating the lifetime of consumables and for monitoring and recording service and qualification information about the device. These functions are called Predictive Performance. They allow you to schedule maintenance procedures based on the actual operating and usage conditions of the device.
Page 121: Performing A Wavelength Validation
• Maintenance and Service Performing a Wavelength Validation A holmium-oxide glass filter is used to validate the wavelength accuracy. The filter can be moved into the light path between the lamp and the flow cell for the wavelength validation. The detector determines the absorption maxima of the filter and compares them to the nominal holmium oxide values that are stored in the detector firmware.
Page 122 • Maintenance and Service To perform a wavelength validation 1. Turn on the flow at the flow rate that will be used for the analysis. 2. Ensure that the shutter is open. 3. Perform the wavelength validation from Chromeleon. 4. If the wavelength validation fails, perform a wavelength calibration from Chromeleon.
Page 123: Replacing The Lamp
• Maintenance and Service Replacing the Lamp When • The lamp is defect • After a defined amount of operating hours of the lamp as a preventive measure • The lamp intensity is too low for demanding applications Parts required Deuterium lamp Tools required Screwdriver, Torx T10...
Page 124 • Maintenance and Service Follow these steps 1. Turn the screws on the lamp house cover counterclockwise until they are loose. Do not remove the screws completely from the lamp house cover. Figure 25: Screws on the lamp house cover 2.
Page 125 • Maintenance and Service 6. Inspect the new lamp for signs of fingerprints and dust. If necessary, clean the lamp with isopropanol and a lint-free tissue before you install it. 7. Align the new lamp with the locating pin. When the lamp is in correct position, push the lamp gently into the lamp house.
Page 126: Flow Cell
• Maintenance and Service Flow Cell This section describes exchange, storage and shipment of LightPipe flow cells. To store or ship the flow cell, follow these steps: 1. Flush the flow cell (see section 7.7.1, page 126). 2. Remove the flow cell from the detector and place it in its packaging (see section 7.7.2, page 128).
Page 127 • Maintenance and Service Preparations 1. Flush the flow cell with a highly pure solvent that is miscible with isopropanol, such as LC/MS-grade acetonitrile or methanol. Make sure that residual sample components, impurities from the column, aggressive solvents or eluates with salt contents are completely flushed out of the flow cell.
Page 128: Removing The Flow Cell
• Maintenance and Service Performing a manual injection The inject and flush kit can also be used to perform a manual injection of a sample directly into the flow cell. NOTICE Particulate matter from the eluate can deposit in the flow cell and clog it.
Page 129 • Maintenance and Service Preparations 1. If the flow cell is to be stored Flush the flow cell (see section 7.7.1, page 126). 2. Proceed as required: ♦ If a flow cell or diagnostic cell is to be installed Close the shutter in the light path before the flow cell (if not yet done).
Page 130: Installing The Flow Cell
• Maintenance and Service b) Position the cover onto the flow cell opening. Make sure that the cover is in the correct orientation. c) Turn the rotating locks clockwise to a vertical position to close the rotating locks and attach the cover to the flow cell opening.
Page 131 • Maintenance and Service Preparations 1. Proceed as required: If a flow cell is installed ♦ Remove the flow cell from the detector. ♦ If the cover is installed to the flow cell opening on the detector a) Turn the rotating locks on the cover of the flow cell opening on the detector counterclockwise until they are in a horizontal position.
Page 132 • Maintenance and Service Follow these steps 1. Remove the shipping locks carefully on the left and right rear side of the flow cell. TIP Store the shipping locks in the cell packaging to have them easily available when storing or shipping the cell. Figure 28: Rear side of the flow cell Description Identification chip...
Page 133 • Maintenance and Service 3. Carefully insert the flow cell into the flow cell opening in the detector. The flow cell must sit completely in the opening. Figure 29: Inserting the flow cell with opened rotating locks 4. Turn the rotating locks simultaneously clockwise until they are in a vertical position.
Page 134: Back-Flushing The Flow Cell
• Maintenance and Service Back-Flushing the Flow Cell If a LightPipe flow cell shows increased backpressure or even blockage, back-flushing the flow cell at a high flow rate can help to regain the original performance of the flow cell. This can also be performed as a remedy if the light transmittance of the flow cell is reduced due to contamination of the light pipe, causing increased noise and instable baseline.
Page 135 • Maintenance and Service Description Back-flush capillary Inlet line for connection to the pump In-line filter Overpressure line to the waste T piece Viper connector for connection to the flow cell Back-flush waste line Back-flush plug • Back-flushing agent to remove the blockage in the flow cell, such as highly pure (e.g.
Page 136 • Maintenance and Service Determining the required pump flow NOTICE Flow cells are highly sensitive to high pressures. Even if the pressure exceeds the upper limit for a very short time only, the flow cell may be permanently damaged. Do not connect the flow cell to the pump for this pump flow determination procedure.
Page 137 • Maintenance and Service 7. Stop the pump flow. 8. Flush the T piece (see section Flushing the T piece below). Flushing the T piece 1. Remove the back-flush plug from the T piece. 2. Connect the back-flush waste line to the back-flush capillary and route the free end of the tubing to waste.
Page 138 • Maintenance and Service Back-flushing 1. Disconnect the back-flush waste line from the back-flush capillary. 2. Connect the Viper connector to the T piece. 3. Connect the back-flush waste line to the flow cell inlet. 4. Route the free end of the back-flush waste line to waste. 5.
Page 139 • Maintenance and Service Forward flushing 1. Switch the capillary connections on the flow cell: Connect the Viper connector from the back-flush capillary to the flow cell inlet and the back-flush waste line to the flow cell outlet. Figure 35: Setup for forward flushing Description To the outlet of the pump Back-flush waste line...
Page 140 • Maintenance and Service Ending the flushing procedure 1. Stop the pump flow. 2. Disconnect the back-flush components: a) Disconnect the Viper connector of the back-flush capillary and the back-flush waste line from the flow cell. b) Disconnect the inlet line from the pump. c) Remove the overpressure line and back-flush waste line from the waste.
Page 141: Diagnostic Cell
• Maintenance and Service Diagnostic Cell When problems with the baseline noise, baseline drift or during wavelength validation occur, install the diagnostic cell and use it to identify the cause: • If the problems disappear with the diagnostic cell The problems are caused by the LightPipe flow cell that was previously installed or the flow path or components in the flow path before the detector.
Page 142 • Maintenance and Service NOTICE—Flow Cell Opening The optical ports and the contact pad for the identification chip in the flow cell opening are sensitive to electrostatic discharge, contamination and scratches. Do not touch any surfaces or optical ports in the flow cell opening. 3.
Page 143: Removing The Diagnostic Cell
• Maintenance and Service 7.9.2 Removing the Diagnostic Cell Preparations If no flow cell is to be installed afterward • Turn off the UV lamp. • If a flow cell is to be installed afterward Close the shutter in the light path before the diagnostic cell. Follow these steps 1.
Page 144: Replacing The Waste Line
• Maintenance and Service 7.10 Replacing the Waste Line NOTICE Backpressures that exceed the specified maximum pressure limit of the flow cell can destroy the flow cell. Observe the following: • Use only the waste line connection that is provided for your detector.
Page 145 • Maintenance and Service Follow these steps 1. When removing the waste line from the tubing guides, be careful not to pull on other tubing in the guides. 2. Disconnect the waste line from the flow cell outlet. 3. Remove the waste line from the top recess of the partition panel: ♦...
Page 146: Replacing The Main Power Fuses
• Maintenance and Service 7.11 Replacing the Main Power Fuses When Blown fuses Parts required Replacement fuses (2 fuses, 3.15AT, 250 V AC, slow-blow, 5 x 20 mm) from Fuses Kit Tools required Slotted screwdriver, any size between 3.3 mm and 5.5 mm is appropriate Preparations WARNING—Electric Shock...
Page 147 • Maintenance and Service Description Main power switch (on/off control) Fuse holder Power-inlet connector 1. Use the screwdriver to remove the fuse holder. 2. Replace the two fuses with new fuses of the specified type and current rating. Always replace both fuses. 3.
Page 148: Updating The Detector Firmware
• Maintenance and Service 7.12 Updating the Detector Firmware When Updating the detector firmware might be required, for example, when a new firmware version is released that adds functionality or solves problems of a previous version. Items required Firmware version/Chromeleon Service Release as appropriate TIP When a new firmware version is released, the new version will be included in the next available Chromeleon Service Release.
Page 149 • Maintenance and Service Follow these steps 1. Start the Chromeleon 7 Instrument Configuration Manager or the Chromeleon 6.8 Server Configuration program. 2. Perform a firmware update from the General tab page in the configuration dialog box for the detector. For details, refer to the Chromeleon Help.
Page 150: Replacing The Doors
• Maintenance and Service 7.13 Replacing the Doors When Damage of door TIP The maintenance procedures do not require that you remove the doors. If this should ever be required for a specific reason or procedure, follow the related steps in this section. Parts required Replacement door Follow these steps...
Page 151 • Maintenance and Service 4. Insert the hinges in the groove, by pushing up and slightly turning the door. 5. Push the door downward to lock it in place. You can close the door only when it is properly installed. Vanquish Diode Array Detectors (VH-D10) Operating Manual Page 151...
Page 152: Transporting Or Shipping The Detector
• Maintenance and Service 7.14 Transporting or Shipping the Detector If you want to transport the detector to a new location or if you need to ship the detector, first prepare the detector for transport and then move or ship the detector as required. Follow the instructions in this section. Observe the following safety guidelines: CAUTION—Heavy Load, Bulky Device The detector is too heavy or bulky for one person alone to handle safely.
Page 153: Preparing The Detector For Transport
• Maintenance and Service 7.14.1 Preparing the Detector for Transport To prepare the detector for transport, follow these steps: 1. Perform a long-term shut down of the detector. See section 6.8.2 Long-Term Shutdown, page 107. 2. Turn off the detector with its main power switch and disconnect the power cord.
Page 154: Transporting The Detector To A New Location
• Maintenance and Service 7.14.2 Transporting the Detector to a New Location To transport the detector to a new location, follow these steps: 1. Observe the notes for handling and lifting the detector safely. 2. Transport the detector to the new location. 3.
Page 155: Shipping The Detector
• Maintenance and Service 7.14.3 Shipping the Detector To ship the detector, follow these steps: 1. Follow the unpacking instructions in this manual in the reverse order. Use only the original packing material and shipping container. If the original shipping container is not available, appropriate containers and packing material can be ordered from the Thermo Fisher Scientific sales organization.
Page 156 • Maintenance and Service Restarting the Detector after Shipping To restart the detector after shipping, follow these steps: 1. Follow the unpacking instruction in this operating manual. 2. Install and set up the detector in the system stack. Follow the instructions on mounting the system stack in the Vanquish System Operating Manual.
Page 157: Slide-In Module
• Maintenance and Service 7.15 Slide-In Module CAUTION—Heavy Load, Bulky Device The detector is too heavy or bulky for one person alone to handle safely. To avoid personal injury or damage to the detector, observe the following guidelines: • Physical handling of the detector, including lifting or moving, requires a team effort of two persons.
Page 158 • Maintenance and Service 2. Push all tubing and capillaries, which are present in the tubing chase of the Vanquish system modules, into the tubing chase. Otherwise, you will not be able to remove the slide-in module properly from the enclosure in the next step.
Page 159: Installing The Slide-In Module
• Maintenance and Service NOTICE The packaging for the slide-in module differs from the packaging for the complete module. Shipping the slide-in module or the detector incorrectly leads to damage on the device. Always pack and ship the slide-in module and the detector in the respective, dedicated packaging.
Page 160 • Maintenance and Service Follow these steps 1. Unpack the slide-in module. Remove any foam spacers from the module. 2. Push all tubing and capillaries, which are present in the tubing chase of the Vanquish system modules, into the tubing chase. Otherwise, you will not be able to insert the slide-in module properly into the enclosure in the next step.
Page 161 • Maintenance and Service 5. Set up the slide-in module: a) To connect the slide-in module and to set up flow connections, follow the instructions in the Installation chapter in this operating manual. b) To prepare the slide-in module for first-time operation, follow the instructions in the Preparing the Detector for Operation section in this operating manual.
Page 162 • Maintenance and Service Page 162 Vanquish Diode Array Detectors (VH-D10) Operating Manual...
Page 163: Troubleshooting
• Troubleshooting 8 Troubleshooting This chapter is a guide to troubleshooting issues that may arise during operation of the detector. Vanquish Diode Array Detectors (VH-D10) Operating Manual Page 163...
Page 164: General Information About Troubleshooting
• Troubleshooting General Information about Troubleshooting The following features help you to identify and eliminate the source for problems that may arise during operation of the detector. TIP For information about operating issues that might occur during the operation of a Vanquish system, refer to the Vanquish System Operating Manual.
Page 165 • Troubleshooting Chromeleon Audit Trail Messages If the device firmware detects a problem, the problem is reported to the Chromeleon software. The Chromeleon software logs information about all events related to instrument operation for the current day in an Audit Trail. The Audit Trail is named with the current date, using the format yyyymmdd.
Page 166 • Troubleshooting Firmware Failure If a firmware failure occurred during operation of the module, an exception log has been created about the processes during the firmware failure. The firmware sends the exception log to the Audit Trail when the module is connected in Chromeleon. In this case, observe the following: •...
Page 167: Messages
• Troubleshooting Messages The table lists the most frequently observed messages for the detector and provides troubleshooting assistance. Each message consists of a code number and a text. The code number is the unique identifier for the problem while the wording may change. Note the following: •...
Page 168 • Troubleshooting Message and Code Description and Remedial Action Code 36 Download failed The firmware download has not been successful. Repeat the download. Code 37 Download firmware The firmware download has not been successful. The firmware is not mismatch suitable for the detector. Verify that the correct firmware file was selected.
Page 169 • Troubleshooting Message and Code Description and Remedial Action Code 3132 Holmium validation Repeat the validation. If the message still appears, proceed as follows: failed – wavelength outside • Perform a calibration and repeat the validation again. limits. • Ensure that the flow cell is installed properly. Remove and install the flow cell.
Page 170 • Troubleshooting Message and Code Description and Remedial Action Code 3164 Data transfer error Check the USB connection. Use only the USB cables provided by Thermo from detector to PC – check Fisher Scientific for the detector. Avoid CPU-intensive and time-consuming USB connection.
Page 171: Checking The Flow Cell
• Troubleshooting Checking the Flow Cell When Related messages that require a flow cell check appear in the Audit Trail. For the messages that require checking the flow cell, see section 8.2 Messages, page 167. Follow these steps 1. Check that the flow cell is installed properly. Remove and install the flow cell.
Page 172: Resolving Leakage
• Troubleshooting Resolving Leakage When The leak sensor is wet. The leak sensor reports leakage. Parts required Replacement part as required Additional items required Cloth or tissue Preparations When resolving leakage, observe the safety guidelines and general rules for maintenance and service as presented in chapter 7 Maintenance and Service, page 111.
Page 173: Specifications
• Specifications 9 Specifications This chapter provides the physical and performance specifications, including information about the materials used in the flow path of the detector. Vanquish Diode Array Detectors (VH-D10) Operating Manual Page 173...
Page 174: Performance Specifications
• Specifications Performance Specifications 9.1.1 Detector The detector performance is specified as follows: Type Specification Optical design • Single-beam, reverse-optics design with concave holographic grating • High Numerical Aperture (NA) achromatic optics • 1024-element photodiode array Light source Deuterium lamp for UV and VIS wavelength range (30 W) Wavelength range 190 –...
Page 175 • Specifications Type Specification Noise <± 3 µAU at 230 nm ASTM <± 10 µAU at 520 nm ASTM Reference conditions • Wavelength: as listed; bandwidth 4 nm; reference wavelength not used; slit width: 4 nm • Time constant: 2 sec (response time = 2.2 x time constant as proposed in ASTM).
Page 176 • Specifications Type Specification See the Specifications for the flow cells. Materials in the flow path NOTICE For information about the chemical resistance of materials refer to the technical literature. Solvent and additive information section 2.4, page Safety features Power-up check of optics, cooling fans, motors and electronics Leak detection and safe leak handling Good Laboratory Practice (GLP) Predictive Performance functions for scheduling maintenance...
Page 177: Lightpipe Flow Cells
• Specifications 9.1.2 LightPipe Flow Cells The flow cell performances are specified as follows: Type 10 mm Fused-Silica LightPipe Flow 60 mm Fused-Silica LightPipe Flow Cell Specification Cell Specification Design Light pipe Light pipe Path length 10 mm 60 mm Illuminated volume 2 µL 13 µL...
Page 178: Physical Specifications
• Specifications Physical Specifications The physical conditions of the detector are specified as follows: Type Specification Range of use Indoor use only Ambient operating temperature 5 – 35 °C Ambient storage temperature -20 °C – 45 °C Ambient humidity 20 - 80% relative humidity (non-condensing) Ambient storage humidity Maximum 60% relative humidity (non-condensing) Pollution degree...
Page 179: Accessories, Consumables And Replacement Parts
• Accessories, Consumables and Replacement Parts 10 Accessories, Consumables and Replacement Parts This chapter describes the standard accessories that are shipped with the detector and the accessories that are available as an option. This chapter also provides information for reordering consumables and replacement parts.
Page 180: General Information
• Accessories, Consumables and Replacement Parts 10.1 General Information The detector must be operated only with the replacement parts and additional components, options, and peripherals specifically authorized and qualified by Thermo Fisher Scientific. Accessories, consumables, and replacement parts are always maintained at the latest technical standard.
Page 181: Ship Kit
• Accessories, Consumables and Replacement Parts 10.2 Ship Kit The ship kit includes the items listed in the table. The kit content is subject to change and may vary from the information in this manual. See the content list included in the kit for the most recent information about the kit content at the time when the detector is shipped.
Page 182: Optional Accessories
• Accessories, Consumables and Replacement Parts 10.3 Optional Accessories Item Part No. Remarks LightPipe flow cell, 6083.0100 For installation instructions, see section 5.5.3, page standard, path length 10 mm LightPipe flow cell, high 6083.0200 For installation instructions, see section 5.5.3, page sensitivity, path length 60 mm LightPipe diagnostic cell...
Page 183: Consumables And Replacement Parts
• Accessories, Consumables and Replacement Parts 10.4 Consumables and Replacement Parts Lamp Description Part No. Deuterium lamp 6083.1110 Capillaries and tubing for LightPipe flow cells Description Part No. Capillary, 0.10 mm I.D., 300 mm length, insulated, for connection to 6083.2405 the column compartment Capillary, 0.13 mm I.D., 350 mm length, insulated, for connection to 6083.2410...
Page 184 • Accessories, Consumables and Replacement Parts Power cords Description Part No. Power cord, Australia 6000.1060 Power cord, China 6000.1080 Power cord, Denmark 6000.1070 Power cord, EU 6000.1000 Power cord, India, SA 6000.1090 Power cord, Italy 6000.1040 Power cord, Japan 6000.1050 Power cord, UK 6000.1020 Power cord, USA...
Page 185: Appendix
• Appendix 11 Appendix This chapter provides additional information about compliance and UV cutoff wavelengths. Vanquish Diode Array Detectors (VH-D10) Operating Manual Page 185...
Page 186: Compliance Information
• Appendix 11.1 Compliance Information 11.1.1 Declarations of Conformity CE Declaration of Conformity The device has satisfied the requirements for the CE mark and is compliant with the applicable requirements. cTUVus Compliance The cTUVus label on the device indicates that the device has satisfied the requirements for the cTUVus mark.
Page 187: Weee Compliance
• Appendix 11.1.2 WEEE Compliance This product is required to comply with the European Union’s Waste Electrical & Electronic Equipment (WEEE) Directive 2002/96/EC. It is marked with the following symbol: Figure 41: WEEE symbol Thermo Fisher Scientific has contracted with one or more recycling or disposal companies in each European Union (EU) Member State, and these companies should dispose of or recycle this product.
Page 188: Fcc Compliance
• Appendix 11.1.3 FCC Compliance This equipment has been tested and found to comply with the limits for a Class A digital device, pursuant to part 15 of the U.S. FCC Rules. These limits are designed to provide reasonable protection against harmful interference when the equipment is operated in a commercial environment.
Page 189: Uv Cutoff Wavelengths Of Solvents
• Appendix 11.2 UV Cutoff Wavelengths of Solvents The UV cutoff wavelength is the minimum effective wavelength for the measurement. The mobile phase composition affects its UV cutoff wavelength. In general, mobile phases are solvents, such as, water, acetonitrile, methanol, or other substances. They may also contain salts, such as sodium hydroxide (NaOH).
Page 190 • Appendix Page 190 Vanquish Diode Array Detectors (VH-D10) Operating Manual...
Page 191: Index
• Index 12 Index Vanquish Diode Array Detectors (VH-D10) Operating Manual Page 191...
Page 192 • Index 3D field ..............97 data collection rate ......... 95, 101 guidelines ............102 decontamination ........... 118 diagnostic cell ............141 accessories ............179 disk space .............. 101 optional ............182 door................. 51 ship kit .............. 181 open ..............51 acquisition off ............
Page 193 • Index identification chip peak match ............101 flow cell .............. 34 peak resolution ............. 101 lamp ..............36 peak width ............96, 101 installation .............. 45 optimization ............. 102 interior view ............33 pH range ..............26 Interlink ..............54 photodiode bunching ..........
Page 194 • Index shutdown long-term ............107 transport ............... 152 short-term ............106 troubleshooting ............ 163 shutter ..............94 tubing ..............64 signal channel ............94 tubing bracket ............63 signal words ............16 tubing chase ............63 slide-in module ............. 157 tubing guides ............
Page 195 • Index Vanquish Diode Array Detectors (VH-D10) Operating Manual Page 195...
Page 196 www.thermofisher.com Thermo Fisher Scientific Inc. 168 Third Avenue Waltham Massachusetts 02451...

ThermoFisher Scientific Vanquish VH-D10 Operating Manual

1 Using this Manual

2 Safety

3 Detector Overview

4 Unpacking

5 Installation

6 Operation

7 Maintenance and Service

8 Troubleshooting

9 Specifications

10 Accessories, Consumables and Replacement Parts

11 Appendix

12 Index

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