Fluigent LineUp Series Quick Start Manual
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Fluigent LineUp Series Quick Start Manual

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USER'S
MANUAL
LINEUP
SERIES
TM

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Summary of Contents for Fluigent LineUp Series

  • Page 1 USER’S MANUAL LINEUP SERIES...
  • Page 2 QUICK START GUIDE Push-Pull Push-Pull LineUp Flow EZ LineUp Push-Pull Pressure controller Pressure controller The quick start guide is a forward to the LineUp Aria is a perfusion system which automates perfusion or timed Series user's manual injection protocols. It allows for the sequential delivery of up to 10 different solutions at the desired flow rate into a microfluidic chip, perfusion chamber or petri dish.
  • Page 3 QUICK START GUIDE An overview of pressure-based microfluidic control Supplied (inlet) pressure from pump or compressed air supply Power supply to the Flow EZ / Push-Pull pressure controller Regulated (outlet) pressure from pressure controller to reservoir Pressurized reservoir P-CAP Microfluidic set-up in example a microfluidic chip The Flow ...
  • Page 4 QUICK START GUIDE What is needed Contained in the package Flow EZ or Push-Pull Outlet tubing (OD 4mm) *OD: Outer diameter P-CAP Kit or Fluiwell Kit (sold separately) P-CAP (or Fluiwell) reservoir Fluidic tubing Fluiwell: for 15mL or 50mL reservoirs Outer diameter and inner diameter P-CAP for 2mL, 15mL or 50mL reservoirs depending on the Kit...
  • Page 5 QUICK START GUIDE LineUp supply Kit (sold separately) Inlet tubing (OD: 6mm) *OD: Outer diameter Power supply (24VDC) Power supply to Sub-D adaptor...
  • Page 6 QUICK START GUIDE Power on To wall socket Once powered on, the Flow EZ TM  Push-Pull will display the "Inlet  pressure  check window", giving the  measured pressure at the inlet  (P  inlet) and the supply pressure  required to operate the pressure controller  (P target). For example, left shows a 1000 mbar range Flow EZ P target : 1100 mbar P inlet : 0 mbar Note : For the 7000 mbar range as well negative...
  • Page 7 Flow  EZ   ranges  Different  -800 require  different  supply  -345 pressures (shown to the right). -800 Note : If one intends to use compressed gas other than air, or if the pressure supply is out of the pressure range requirements, please contact Fluigent.
  • Page 8 QUICK START GUIDE Push-Pull specificities Push-Pull Flow  EZ The  module works the same way as other   modules, pressure except that it can output (above atmospheric pressure) vacuum (below atmospheric pressure) through the same port. Push-Pull output (mbar) Required pressure supply (mbar) 1000 1100 -800...
  • Page 9 QUICK START GUIDE Push-Pull module can be combined with other positive pressure LineUP Flow EZ modules the same way as a higher pressure modules, p l e a s e 1 b a r . I f co n n e c t i n g t o A d a p t c o n s i d e r a d d i n g a n m o d u l e t o r e d u c e t h e...
  • Page 10 QUICK START GUIDE Adjust the supplied pressure (A 1000 mbar range Flow EZ is shown as an example) Adjust the pressure from your pressure source The P inlet value The P inlet value is displayed in is displayed in green not set When connecting the pressure supply, if its value (P inlet) is required value...

  • Page 11: Operation Window

    QUICK START GUIDE Operation window Pause button Product name and pressure range Press to bring outlet pressure to zero Press again to Pmeas recover pressure Measurement of Hold to enter sleep the pressure in the mode reservoirs Pcmd Unit can be changed using the User-defined menu...

  • Page 12: Pressure Sensor Calibration

    QUICK START GUIDE Pressure sensor calibration An example of Pmeas before calibration Calibrating ... Press "Menu" Use the dial (turn and press) to select "Pressure calibrate" Wait a few seconds The calibration is done Note: Due to the high sensitivity of the internal pressure sensor, one may observe some small fluctuations of the measured pressure (Pmeas), even after the calibration is complete.
  • Page 13 Aria is a perfusion system which automates perfusion or timed FLOW UNIT One can connect a (Fluigent flow sensor) between the injection protocols. It allows for the sequential delivery of up to 10 reservoir and the microfluidic setup. (See page 22 for more details) different solutions at the desired flow rate into a microfluidic chip, perfusion chamber or petri dish.
  • Page 14 QUICK START GUIDE When done with the Flow EZ / Push-Pull Flow EZ or Push-Pull, hold When one has finished using the the "P=0" button in order to put the device into sleep mode. If one needs to disconnect the power supply (e.g. to move or store the unit), please make sure the Flow EZ or Push-Pull is already in sleep mode before disconnecting the power.
  • Page 15 The Flow EZ or Push-Pull is ready to be used, Simply turn the dial to start. user's manual in on the next page: LineUp series Allowing one to get the most out of the devices.
  • Page 17 LINEUP SERIES USER’S MANUAL PRODUCT OVERVIEW SETTING UP A faster way to change the pressure Add a FLOW UNIT Flow rate control Inject a volume Flow-related setting Liquid type SYSTEM EXPANSION Create a multi-channel configuration Mixing different pressure range LineUp Adapt COMPUTER CONNECTION LineUp Link...
  • Page 18 PRODUCT OVERVIEW LineUp Flow EZ Pressure controller FLOW UNIT port to the flow sensor Pressure supply transmission used only in multi-channel configuration Pressure outlet to reservoir Pressure inlet from pump or compressed air supply...
  • Page 19 PRODUCT OVERVIEW LineUp Push-Pull Pressure controller FLOW UNIT port to the flow sensor Pressure supply transmission used only in multi-channel configuration Pressure outlet to reservoir Positive pressure inlet from pump or compressed air supply Vacuum inlet from vacuum pump...
  • Page 20 PRODUCT OVERVIEW Change pressure command Set/Apply pressure Navigate Command set in menu in menu Unlock button used only in multi-channel configuration P=0 button press once to set pressure to 0, press again to return to original pressure, hold to put into sleep mode Left button press to rotate the display/go back Right button...
  • Page 21 SETTING UP A faster way to change the pressure Classic way: Turn dial in the "Operation window" to immediately change the requested pressure, this command is continuously Flow EZ or Push-Pull. applied by the ON/OFF way: Click on the dial, set the pressure requested pressing the dial faster...
  • Page 22 SETTING UP Add a FLOW UNIT The FLOW UNIT is Fluigent's flow rate sensor. When a FLOW UNIT is added, one can use the Flow EZ or Push-Pull to: • Monitor the flow rate in the microfluidic set-up Directly control the flow rate locally •...
  • Page 23 SETTING UP Connect the FLOW UNIT to the Flow EZ or Push-Pull dedicated port Use the on the back of the pressure controller to connect the flow sensor. Once a FLOW UNIT is connected, the automatically detects device it and the "Operation window" will flow rate measurement.

  • Page 24: Flow Rate Control

    Abnormal flow rates may reflect problems in the microfluidic set-up (leakage, clogging, etc.) See Fluigent FAQs on www.fluigent.com for more information. Direct flow rate control works only when the value of measured flow rate (Qmeas) is positive.
  • Page 25 Target Push-Pull is in stand-alone mode. When volume time connected to a PC, the injection operations need to be initiated by Fluigent software. Back Example where 200µL is Example where 1:20 min set as the target volume is set as the target time...
  • Page 26 SETTING UP Flow rate settings Response time reflects how fast the pressure controller regulates the flow to match the flow rate order defined by the user. By default, the response time is set to 2 seconds. In some circumstances where a smoother response is preferred, one can set a longer response time.
  • Page 27 SETTING UP Liquid type set manually The type of liquid is using the menu. Selecting the corresponding liquid type makes the flow rate measurements more accurate: water-based solutions: select "H2O" (water) oil-based solutions: select "iPA" (isopropanol) Note: In the case of range M FLOW UNIT, additional liquid types are possible: •...

  • Page 28: System Expansion

    SYSTEM EXPANSION Creating a multi-channel configuration The Flow EZ and Push-Pull have a hot plug & play feature: One can add or remove a module while the device is running. Add a channel Remove a channel automatically passed The power and pressure supply are to the 8 modules can be stacked newly added pressure controller.
  • Page 29 SYSTEM EXPANSION Mixing different pressure ranges If the ranges have the same required pressure supply, one can directly lining up connect them by the modules. 1000 mbar range Required supply pressure: 1100 mbar 1000 mbar range Required supply pressure: 1100 mbar 345 mbar range Required supply pressure: 1100 mbar...
  • Page 30 SYSTEM EXPANSION LineUp Adapt different required supply pressures, one can If the ranges have Adapt still have them in the same chain by inserting an module between them. LineUp Adapt is a manual pressure reduces the supply pressure regulator, it passed from the left side to the lower supply pressure Flow EZ...
  • Page 31 Th e L i n k a l l o w s t h e p r e s s u r e c o n t r o l l e r t o b e n e f i t f u l l a d va n ce d f u n c t i o n a l i t i e s f r o m Fluigent's sof tware s u i t e .

  • Page 32: Shutdown Procedure

    SHUTDOWN PROCEDURE Before disconnecting any FLOW UNIT, please be sure to clean cleaning protocols. it with appropriate solvent detailed in the Doing so will prevent any blockage and prolong its service life. Press and hold (for a few seconds) the "P=0" button in order to: •...

  • Page 33: Additional Features

    ADDITIONAL FEATURES One can use the Flow EZ Push-Pull different positions: Horizontal Angled Vertical "Chain  to  Chain  Kit"  (sold  separately)  to  give  more  Use  the  flexibility when placing the multi channel configuration on the lab  bench.

  • Page 34: Frequently Asked Questions

    FREQUENTLY ASKED QUESTIONS The  Flow  EZ or Push-Pull  cannot  achieve the requested pressure or flow rate, what should I do? air tight no leaks. Verify the set-up is and there are pressure supply required pressure Verify that the is providing the (Flow EZ Push-Pull cannot provide an outlet pressure greater than supplied pressure).
  • Page 35 Flow EZ Push- Some older by the Pull. In this case, please contact Fluigent at support@fluigent.com Can I use the LineUp Push-Pull even if one of the two inlets is not supplied, as a regular positive or negative Flow EZ...

  • Page 36: Warranty Terms

    What this warranty covers This warranty is granted by Fluigent and applies in all countries. The Fluigent product is guaranteed for one year from the date of delivery at the laboratory against defects in materials and workmanship. If found to be defective within the warranty period, the Fluigent product will be repaired or replaced free of charge.

  • Page 37: Technical Support

    +33 1 77 01 82 65 Fluigent Inc. +1 (978) 934 5283 Fluigent GmbH +49 3641 277 652 For a fully detailed FAQ for all Fluigent products, please visit: http://www.fluigent.com/faqs/ Interested in Fluigent products ? complete Fluigent product line application...
  • Page 40 VERSION MAR. 2020...

This manual is also suitable for:

Lineup push-pullLineup flow ez

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