Download
Share
Print this page
  1. Manuals
  2. Brands
  3. Fluigent Manuals
  4. Controller
  5. LineUp Series
  6. User manual

Fluigent LINEUP Series User Manual

Hide thumbs Also See for LINEUP Series:

Advertisement

Quick Links

Download this manual
USER'S
MANUAL
LINEUP
SERIES
TM

Advertisement

loading

Related Manuals for Fluigent LINEUP Series

Summary of Contents for Fluigent LINEUP Series

  • Page 1 USER’S MANUAL LINEUP SERIES...
  • Page 2 QUICK START GUIDE Push-Pul Push-Pull LineUp Flow EZ LineUp Push-Pull Pressure controller Pressure controller Aria is a perfusion system which automates perfusion or timed The quick start guide is a forward to the LineUp Series user's manual injection protocols. It allows for the sequential delivery of up to 10 different solutions at the desired flow rate into a microfluidic chip, perfusion chamber or petri dish.
  • Page 3 QUICK START GUIDE Supplied (inlet) pressure from pump or compressed air supply Power supply to the Flow EZ / Push-Pull pressure controller Regulated (outlet) pressure from pressure controller to reservoir Pressurized reservoir P-CAP Microfluidic set-up in example a microfluidic chip The Flow EZ / Push-Pull regulates the externally supplied (inlet) pressure to a user -defined pressure with high precision.
  • Page 4 QUICK START GUIDE Contained in the package Flow EZ or Push-Pull Outlet tubing (OD 4mm) *OD: Outer diameter P-CAP Kit or Fluiwell Kit (sold separately) P-CAP (or Fluiwell) reservoir Fluidic tubing Fluiwell: for 15mL or 50mL reservoirs Outer diameter and inner diameter P-CAP for 2mL, 15mL or 50mL reservoirs depending on the Kit...
  • Page 5 QUICK START GUIDE LineUp supply Kit (sold separately) Inlet tubing (OD: 6mm) *OD: Outer diameter Power supply (24VDC) Power supply to Sub-D adaptor...
  • Page 6 QUICK START GUIDE To wall socket Once powered on, the Flow EZ Push-Pull will display the "Inlet pressure check window", giving the measured pressure at the inlet (P inlet) and the supply pressure required to operate the pressure controller (P target). For example, left shows a 1000 mbar range Flow EZ P target : 1100 mbar...
  • Page 7 Flow EZ ranges -800 require different supply -345 pressures (shown to the right). -800 Note : If one intends to use compressed gas other than air, or if the pressure supply is out of the pressure range requirements, please contact Fluigent.
  • Page 8 QUICK START GUIDE Push-Pull module works the same way as other Flow EZ modules, except that it can output pressure (above atmospheric pressure) vacuum (below atmospheric pressure) through the same port. Push-Pull output (mbar) Required pressure supply (mbar) 1000 1100 -800 -800 To do so, it needs...
  • Page 9 QUICK START GUIDE Push-Pull module can be combined with other positive pressure LineUP modules the same way as a Flow EZ 1 bar. If connec ting to h i g h e r p re s s u re m o d u l e s , please c o n s i d e r a d d i n g a n A d a p t...
  • Page 10 QUICK START GUIDE (A 1000 mbar range Flow EZ is shown as an example) Adjust the pressure from your pressure source The P inlet value The P inlet value is displayed in is displayed in green When connecting the pressure supply, if its value (P inlet) is not set required value (P target), the...
  • Page 11 QUICK START GUIDE Product name and Pause button pressure range Press to bring outlet pressure to zero Press again to Pmeas recover pressure Measurement of Hold to enter sleep the pressure in the mode reservoirs Pcmd Unit can be changed using the User-defined menu pressure command...
  • Page 12 QUICK START GUIDE An example of Pmeas before calibration Calibrating ... Press "Menu" Use the dial (turn and press) to select "Pressure calibrate" Wait a few seconds The calibration is done Note: Due to the high sensitivity of the internal pressure sensor, one may observe some small fluctuations of the measured pressure (Pmeas), even after the calibration is complete.
  • Page 13 Aria is a perfusion system which automates perfusion or timed One can connect a FLOW UNIT (Fluigent flow sensor) between the injection protocols. It allows for the sequential delivery of up to 10 reservoir and the microfluidic setup. (See page 22 for more details) different solutions at the desired flow rate into a microfluidic chip,...
  • Page 14 QUICK START GUIDE When one has finished using the Flow EZ or Push-Pull, hold the "P=0" button in order to put the device into sleep mode. If one needs to disconnect the power supply (e.g. to move or store the unit), please make sure the Flow EZ or Push-Pull is already in sleep mode before disconnecting the power.
  • Page 15 The Flow EZ or Push-Pull is ready to be used, Simply turn the dial to start. user's manual in on the next page: Allowing one to get the most out of the LineUp series devices.
  • Page 17 LINEUP SERIES USER’S MANUAL PRODUCT OVERVIEW SETTING UP A faster way to change the pressure Add a FLOW UNIT Flow rate control Inject a volume Flow-related setting Liquid type SYSTEM EXPANSION Create a multi-channel configuration Mixing different pressure range LineUp Adapt COMPUTER CONNECTION LineUp...
  • Page 18 PRODUCT OVERVIEW LineUp Flow EZ Pressure controller FLOW UNIT port to the flow sensor Pressure supply transmission used only in multi-channel configuration Pressure outlet to reservoir Pressure inlet from pump or compressed air supply...
  • Page 19 PRODUCT OVERVIEW LineUp Push-Pull Pressure controller FLOW UNIT port to the flow sensor Pressure supply transmission used only in multi-channel configuration Pressure outlet to reservoir Positive pressure inlet from pump or compressed air supply Vacuum inlet from vacuum pump...
  • Page 20 PRODUCT OVERVIEW Change pressure command Set/Apply pressure Navigate Command set in menu in menu Unlock button used only in multi-channel configuration P=0 button press once to set pressure to 0, press again to return to original pressure, hold to put into sleep mode Left button press to rotate the display/go back Right button...
  • Page 21 SETTING UP Classic way: Turn dial in the "Operation window" to change the requested pressure, this command is immediately continuously applied by the Flow EZ or Push-Pull. ON/OFF way: Click on the dial, set the pressure requested advance, and apply it later by pressing the dial again.
  • Page 22 SETTING UP FLOW UNIT The FLOW UNIT is Fluigent's flow rate sensor. When a FLOW UNIT is added, one can use the Flow EZ or Push-Pull to: • Monitor the flow rate in the microfluidic set-up • Directly control the flow rate locally •...
  • Page 23 SETTING UP Connect the FLOW UNIT to the Flow EZ or Push-Pull Use the dedicated port on the back of the pressure controller to connect the flow sensor. Once a FLOW UNIT is connected, the device automatically detects it and the "Operation window" will display an additional zone including the flow rate measurement.
  • Page 24 fluidic set-up. Abnormal flow rates may reflect problems in the microfluidic set-up (leakage, clogging, etc.) See Fluigent FAQs on www.fluigent.com for more information. Direct flow rate control works only when the value of measured flow rate (Qmeas) is positive.
  • Page 25 Target Push-Pull is in stand-alone mode. When volume time connected to a PC, the injection operations need to be initiated by Fluigent software. Back Example where 200 L is Example where 1:20 min set as the target volume is set as the target time...
  • Page 26 SETTING UP Response time reflects how fast the pressure controller regulates the flow to match the flow rate order def ined by the user. By default, the response time is set to 2 seconds. In some circumstances where a smoother response is preferred, one can set a longer response time.
  • Page 27 SETTING UP The type of liquid is set manually using the menu. Selecting the corresponding liquid type makes the flow rate measurements more accurate: water-based solutions: select "H2O" (water) oil-based solutions: select "iPA" (isopropanol) Note: In the case of range M FLOW UNIT, additional liquid types are possible: •...

  • Page 28: System Expansion

    SYSTEM EXPANSION The Flow EZ and Push-Pull have a hot plug & play feature: One can add or remove a module while the device is running. Add a channel Remove a channel The power and pressure supply are automatically passed to the newly added pressure controller.
  • Page 29 SYSTEM EXPANSION If the ranges have the same required pressure supply, one can connect them by directly lining up the modules. 1000 mbar range Required supply pressure: 1100 mbar 1000 mbar range Required supply pressure: 1100 mbar 345 mbar range Required supply pressure: 1100 mbar Flow EZ...
  • Page 30 SYSTEM EXPANSION If the ranges have different required supply pressures, one can still have them in the same chain by inserting an Adapt module between them. LineUp Adapt is a manual pressure regulator, it reduces the supply pressure passed from the left side to the lower supply pressure needed for the Flow EZ Push-Pull...
  • Page 31 The LINK allows the pressure controller to benef it full advanced functionalities from Fluigent's software suite. Download Fluigent software using the FSI (Fluigent Software Installer) or the provided USB stick. • OxyGEN for real-time control and automation •...
  • Page 32 COMPUTER CONNECTION The Link can communicate with a PC using different kind of port: • USB port communication • TTL port communication • Serial port communication (RS-232 port, detailed in the next part) Lin eUp Link allows to connect the LineUp series modules to...
  • Page 33 COMPUTER CONNECTION (LINK COM) This remote command set is the default set available on the instrument. All commands must be terminated with a <CR>. All decimal values use the point “.” as decimal separator. A query command ends with a question mark “?” for queries. The data column represents the response of the instrument.
  • Page 34 COMPUTER CONNECTION Query Data Function / Response SYST <vendor> Returns the identification string. <instrument> :IDN? SN and VN are in decimal and on 5 <serial number> characters. <version number> Returns the instrument status: :STATUS? <status> 1 = On, 2 = Off Returns information about the <vendor>...
  • Page 35 COMPUTER CONNECTION Query Data Function / Response CHAN Returns information about the instrument at index X <pmin> X:CONF:P? <pmax> pmin = pressure min, in mbar, as an integer <mode> pmax = pressure max, in mbar, as an integer mode = pressure control mode, 0 = fast, 1 = smooth Returns information about the instrument at index X...

  • Page 36: Shutdown Procedure

    SHUTDOWN PROCEDURE Before disconnecting any FLOW UNIT, please be sure to clean it with appropriate solvent detailed in the cleaning protocols. Doing so will prevent any blockage and prolong its service life. Press and hold (for a few seconds) the "P=0" button in order to: •...

  • Page 37: Additional Features

    ADDITIONAL FEATURES One can use the Flow EZ Push-Pull different positions: Horizontal Angled Vertical Use the "Chain to Chain Kit" (sold separately) to give more flexibility when placing the multi channel configuration on the lab bench.

  • Page 38: Frequently Asked Questions

    FREQUENTLY ASKED QUESTIONS The Flow EZ or Push-Pull cannot achieve the requested pressure or flow rate, what should I do? Verify the set-up is air tight and there are no leaks. Verify that the pressure supply is providing the required pressure (Flow EZ Push-Pull cannot provide an outlet pressure greater...
  • Page 39 Flow EZ Push- Pull. In this case, please contact Fluigent at support@fluigent.com Can I use the LineUp Push-Pull even if one of the two inlets is not supplied, as a regular positive or negative Flow EZ...

  • Page 40: Warranty Terms

    What this warranty covers This warranty is granted by Fluigent and applies in all countries. The Fluigent product is guaranteed for one year from the date of delivery at the laboratory against defects in materials and workmanship. If found to be defective within the warranty period, the Fluigent product will be repaired or replaced free of charge.

  • Page 41: Technical Support

    +33 1 77 01 82 65 Fluigent Inc. +1 (978) 934 5283 Fluigent GmbH +49 3641 277 652 For a fully detailed FAQ for all Fluigent products, please visit: http://www.fluigent.com/faqs/ Interested in Fluigent products ? To view the complete Fluigent product line...
  • Page 44 VERSION JAN. 2021...

This manual is also suitable for:

Lineup flow ezLineup push-pull