Calibrating The Flow Rate - Waters 515 HPLC Operator's Manual

4. Press Edit/Enter to access the Head Size submenu.
5. Press the Up or Down key to change the Head size parameter to the
correct value.
6. Press Menu to store the parameter.
7. Press Menu until you are prompted to exit.
8. Press Edit/Enter to exit the Calibrate menu.
9. Check the HPL and LPL parameter settings, and adjust them if
necessary for the new head size.

Calibrating the flow rate

The following procedure describes how to calibrate the pump flow rate. The
Flowrate Calibration parameter value is factory set and does not require
routine adjustment. Adjust the Flowrate Calibration parameter when you
replace a pump head or pump seals.
Tip:
To adjust for differences in eluent compressibility, use only the Comp
parameter; do not adjust the Flowrate calibration parameter. See
for eluent compressibility" on page
Required materials
• 25-mL volumetric flask
• Stopwatch
• Reservoir of degassed methanol connected to the pump inlet
• Methanol-compatible column connected to pump outlet
To calibrate the pump flow rate:
1. Press the Up, Down, or Menu key to display the Flow parameter. Set
Flow to 1.000 mL/min.
2. Direct the outlet of the column into the 25-mL volumetric flask.
3. Simultaneously press the Run/Stop key and start the stopwatch (to time
how long it takes to collect 25 mL).
4. Simultaneously press the Run/Stop key and stop the stopwatch when
you have collected 25 mL of methanol.
3-24.
Calibrating the pump
"Adjusting
4-7