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R-Biopharm RIDA qLine autoBlot User Manual

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Summary of Contents for R-Biopharm RIDA qLine autoBlot

  • Page 1 R-Biopharm AG...
  • Page 2 Failure to comply with the instructions in this manual will void the manufacturer’s warranty and may pose a risk to the user. Ensure all users are conversant with the instrument - contact R-Biopharm AG for any further instrument or training requirements if in any doubt before using the instrument.
  • Page 3 +49 (0) 61 51 - 8102-40 E-mail: info@r-biopharm.de R-Biopharm AG reserves the right to modify its products and services at any time. This guide is subject to change without notice. Although prepared to ensure accuracy, R-Biopharm AG assumes no liability for errors or omissions, or for any damages resulting from the application or use of this information.

  • Page 4: Table Of Contents

    RIDA qLine autoBlot Revision 1.0 | User manual ® Contents Inhaltsverzeichnis 1 Intended use ........................6 2 Introduction ........................6 2.1 Purpose ................................6 2.2 Product Overview .............................. 6 3 Technical Specifications ....................8 4 Safety & General Information ................... 8 4.1 Unpacking and installation ..........................
  • Page 5 RIDA qLine autoBlot Revision 1.0 | User manual ® 6.5.1 Transfer exception handling ......................... 29 6.6 Obtaining work-list information directly from the instrument ................ 30 6.7 Stand-alone Imaging ............................31 6.8 Accessing stored Images ..........................32 6.9 QC-test ................................33 6.10 Automated cleaning routine..........................

  • Page 6: Intended Use

    R-Biopharm AG. Any use other than mentioned above does not apply to the intended use of the system and R-Biopharm AG shall not be liable for any damage resulting from such unintended use. An intended use also includes the compliance with any safety and environmental laws and regulations as well as the instructions in the manual to prevent any damage to the user and the environment.
  • Page 7 The combination of all these features result in the ability to completely automate the processing of R-Biopharm RIDA qLine allergy membranes, starting from sample handling and ending with images of the membranes once ®...

  • Page 8: Technical Specifications

    RIDA qLine autoBlot Revision 1.0 | User manual ® 3 Technical Specifications Instrument type: Stand-alone bench top Processing Capability: 1 to 36 samples per run Processing Time: Typically 3.5 hours for 36 samples Temperature Control: PID under software control Processing Volumes: 10 µL to 1000 µL Dispensing Mode &...
  • Page 9 RIDA qLine autoBlot Revision 1.0 | User manual ® 1. Upon receipt of the instrument, visually inspect the container for any transit damage. Any damage should be recorded prior to opening. 2. Ensure that the wooden container is placed in an upright position before attempting to open. 3.

  • Page 10: Instrument Labelling

    RIDA qLine autoBlot Revision 1.0 | User manual ® 4.2 Instrument Labelling Please observe and respect all caution labels shown on instrument & consumables: 4.3 Detailed personal safety information Warning Avoid touching the instrument with wet hands, and do not attempt to disassemble the instrument.

  • Page 11: Ventilation

    RIDA qLine autoBlot Revision 1.0 | User manual ® The mains switch and mains lead connection is located on the right hand side panel of the instrument. Do not place the instrument too close to any object that might impair emergency disconnection in an emergency, particularly any object to the right side panel of the instrument.

  • Page 12: Mount And Connect The Touchscreen

    RIDA qLine autoBlot Revision 1.0 | User manual ® 4.5.2 Mount and connect the touchscreen Fig. 3 Fig. 4 To attach the support arm, and thus the touch screen to the instrument, 4 screws, 3 washers and two Allen keys must be retrieved from the accessory collection. Using the longest M6x75 screw from the screw set (and appropriate M6 washer), secure the Touchscreen rear clamp to the arm.

  • Page 13: System Start And Initialization Check

    RIDA qLine autoBlot Revision 1.0 | User manual ® 4.5.4 System start and initialization check Once the instrument has been powered and the on-board computer has loaded up, the main software application should automatically launch and begin an initialisation task. This is a task which happens every start-up to ensure that all the core components are fully functional and ready for use.

  • Page 14: Critical Errors

    RIDA qLine autoBlot Revision 1.0 | User manual ® 4.5.5 Critical errors The robotic side of the instrument has its own error handler that is separate from the global software controller. This means that it has its own way of generating and displaying errors. Any time a robotics error is detected;...

  • Page 15: Installation Of Accessories

    RIDA qLine autoBlot Revision 1.0 | User manual ® 5.2 Installation of accessories Fig. 10 The drawing above illustrates the positioning of all the accessories on the deck of the instrument. Further notes on the assembly and placement of the accessories can be found below. Accessory I.

  • Page 16: Instrument Consumables

    RIDA qLine autoBlot Revision 1.0 | User manual ® 5.3 Instrument consumables The only other consumables in addition to the diagnostic kit being used are the disposable plastic tips. The instrument has been designed for use with a specific type of tips, ones which will be provided by the distributor.

  • Page 17: Logging Tab

    A hidden feature on this screen is the ability to unlock a fourth main menu tab called Engineering. This is done by pressing down on the R-Biopharm branding logo for approximately 3 seconds, or until you see the message ‘engineering activated’ as shown in the following screen. The engineering tab can be deactivated by repeating the above steps until a similar ‘engineering has been deactivated’...

  • Page 18: Installed Scripts Tab

    RIDA qLine autoBlot Revision 1.0 | User manual ® If as a user you witness unusual behaviour that you would like to report to your service provider, the instrument logs should be copied using this tool and forwarded along with your report. 6.1.3 Installed scripts tab The installed scripts tab can be found to the right of the logging tab (see Figure 16).

  • Page 19: Activating Engineering

    RIDA qLine autoBlot Revision 1.0 | User manual ® If the instrument has already entered the main menu before an RJ-45 cable is connected, the application will need to be restarted to register it. 6.2.2 Activating Engineering Activate the engineering tab by following the steps outlined in section 6.1.1 upon activating engineering and closing the system menu, the following screen become visible.

  • Page 20: Connecting To The Instrument From Third Party Software

    RIDA qLine autoBlot Revision 1.0 | User manual ® Fig. 20 The instrument comes with two RJ-45 Ethernet ports which mean there are two possible addresses that can be listened to. Each of the two IP addresses represent the IP address the instrument is listening to for messages.
  • Page 21 (in maximum 4) must be entered manually into the qsoft.ini-file. In order to find the qsoft.ini file ® please enter %appdata%\R-Biopharm\qsoft into the address bar of the windows explorer and confirm with the enter key. Please open the qsoft.ini file by double click and go to the section as shown below: Fig.

  • Page 22: Creating A Worklist

    RIDA qLine autoBlot Revision 1.0 | User manual ® Fig. 23 The AutoBlotProtocolversion may not be changed. The AutoBlotSoftwareversion corresponds with the Application version and the AutoBlotFirmwareversion corresponds with the FirmwareVersion shown in the Information tab (see section 6.1.1) 6.4 Creating a worklist In order to create a work-list the autoBlot must be connected with the evaluation software RIDA qLine Soft.
  • Page 23 RIDA qLine autoBlot Revision 1.0 | User manual ® Fig. 25 and then SCAN CAROUSEL Fig.26 After the scanning process the numbers of recognized barcodes are displayed on the touchscreen. Fig. 27 In case the barcode of not all sample tubes are detected, the Lab ID and the position in the carousel of the not detected sample(s) could be entered manually.
  • Page 24 RIDA qLine autoBlot Revision 1.0 | User manual ® If there is no LIS connection select the filter "open". All samples are displayed for which a request exists. For all not displayed LabIDs no request exists. If no request exist for one or more barcodes the work-list cannot be created, and an error message (Timeout) will be displayed.
  • Page 25 RIDA qLine autoBlot Revision 1.0 | User manual ® Fig. 32 navigate forward button After pressing the navigate forward button the allocation of the strips onto the strip holder will be shown. Fig. 33 By navigating ahead by pressing the navigate forward button (denoted by the right-pointing arrow), the user will be taken to a check-list which the user will need to complete;...

  • Page 26: Running A Work-List

    RIDA qLine autoBlot Revision 1.0 | User manual ® This is the core screen that will be visible while the instrument runs the protocol on the troughs and samples; starts with pre-washing and is then followed by sample transfer and strip processing. If the number of strips to be tested after scanning the sample tubes is greater than 36 (maximum number of strips per run), the work list is divided into 1 or more sub-work lists.
  • Page 27 RIDA qLine autoBlot Revision 1.0 | User manual ® Fig. 40 After pressing the navigate forward button the allocation of the strips onto the strip holder will be shown. Fig. 41 By navigating ahead by pressing the navigate forward button (denoted by the right-pointing arrow), the user will be taken to a check-list which the user will need to complete;...
  • Page 28 RIDA qLine autoBlot Revision 1.0 | User manual ® Fig. 44 Fig. 45 After confirming that everything is located correctly within the instrument, pressing navigate forward will immediately lead to the start of the run. This is the core screen that will be visible while the instrument runs the protocol on the troughs and samples;...

  • Page 29: Transfer Exception Handling

    RIDA qLine autoBlot Revision 1.0 | User manual ® Fig. 47 Fig. 48 Fig. 49 The time control should be accurate to within 10 minutes of the actual completion time. The other interactable control on the processing screen is the abort button. Pressing this button will trigger a confirmation pop-up, which when confirmed, will begin the aborting procedure for a work-list.

  • Page 30: Obtaining Work-List Information Directly From The Instrument

    RIDA qLine autoBlot Revision 1.0 | User manual ® When any of the situations listed above arise, the instrument will retry the transfer 2 additional times (making a total of 3 attempts), each time using a new tip. If the initial issue isn’t resolved after the third attempt, the instrument will pause, sound an alarm and bring up a pop-up window as shown in Figure Fig.

  • Page 31: Stand-Alone Imaging

    RIDA qLine autoBlot Revision 1.0 | User manual ® the logs and images (if available) to a selected USB device. Entries can be deleted by pressing and holding down on the name of the work-list that should be deleted. Fig. 53 When users save work-list history data to a USB device, folders are created with the following structure: DEVICE\\AutoBlot_Assay_Records\\DATE\TIME\\WorklistName\\...

  • Page 32: Accessing Stored Images

    RIDA qLine autoBlot Revision 1.0 | User manual ® Fig. 55 After pressing START SCAN, the screen below will become active. This screen will inform the user of the progress of the scan, along with an estimated time before the procedure finishes. If the user wishes to cancel the scan and discard all associated data, pressing the ABORT button once will park the instrument and return the user to the main menu.

  • Page 33: Qc-Test

    RIDA qLine autoBlot Revision 1.0 | User manual ® Fig. 57 Similarly to the other features within the instrument software, entries from this list can be deleted by pressing and holding down on a selected entry. Holding down for long enough will bring up a pop-up that asks if the user would like to delete the selected entry.
  • Page 34 RIDA qLine autoBlot Revision 1.0 | User manual ® Fig. 59 At this point, the user is expected to perform the following: 1. Remove the wash bottle from the instrument 2. Load a cleaning solution (e.g. 4 % bleach or 4 % SDS) into the wash bottle and fastening the caps back on correctly.

  • Page 35: Instrument Shutdown

    RIDA qLine autoBlot Revision 1.0 | User manual ® Fig. 61 Once the pop-up is closed, the instrument will once more flush and soak, while also back-priming the DI water in the system; the task is reflected on screen, as shown in Figure 62. Fig.

  • Page 36: Troubleshooting

    RIDA qLine autoBlot Revision 1.0 | User manual ® Fig. 63 When the instrument has been completely powered down, the following steps should be carried out to the instrument and its accessories: Step To-do Remove the Wash and Reagent bottles from their respective holders. Cap the bottles if required for future use and return to the fridge.

  • Page 37: Fluidics

    If the instrument fails to perform any or all of these tasks, then there is a fault, which needs to be rectified. Please contact your local representative or R-Biopharm AG for further help. 8.1.5 Critical error messages If critical instrument error messages, as described in section 4.5.5, keep arising during initialisation or at any other step with the instrument, the code sequence for each entry should be recorded and sent to your local representative for further inspection.

  • Page 38: Daily Cleaning

    The general maintenance and the calibration of the pumps is described in detail in the autoBlot Service manual. Only by R-Biopharm AG or associated trained service personnel may authorised repairs and servicing be carried out. For further details of instrument or to arrange for training to be given on the instrument, contact R-...

  • Page 39: Disposal

    Please note the serial number of the instrument (found on the back) below for future reference: Instrument serial number: Warranty Statement R-Biopharm AG warrants that each product described herein will be free from defects in materials and workmanship for a period of one year from the date of delivery. R-Biopharm AG agrees, as its sole...
  • Page 40 R- Biopharm AG in any way. In no event will R-Biopharm AG be liable for any special, incidental, or consequential damages resulting from use or malfunction of this product or the equipment or system...

  • Page 41: Appendix

    RIDA qLine autoBlot Revision 1.0 | User manual ® 12 Appendix 12.1 Appendix II: Synopsis of symbols used in manual an on instrument Symbol Colour Publication Description Placement Symbol & Outline: Instrument Serial IEC 60417 – 5032 Alternating Current Black Label Symbol &...

  • Page 42: Touchscreen Icons

    RIDA qLine autoBlot Revision 1.0 | User manual ® 12.2 Touchscreen icons Icon Function Location View, select then run a work-list Home Screen (Worklist Tab) Access the work-list history menu Home Screen (Worklist Tab) Initiate the standalone imaging Home Screen (Imaging Tab) View latest standalone images Home Screen (Imaging Tab) Initiate the automated cleaning...

  • Page 43: Appendix Ii: Autoblot Installation Checklist

    RIDA qLine autoBlot Revision 1.0 | User manual ® 12.3 Appendix II: autoBlot installation checklist Instrument serial number: Customer name:: Address: Phone: Fax: : E-mail address: Did the instrument arrive in good condition? If no, provide details: Were the following accessories present? 1 x Touch Screen, Mounting Arm &...
  • Page 44 Revision 1.0 | User manual ® Any other comments: Representative Name: Representative Address: Representative Signature: Date: Customer Signature: Date: Customer Print Name: Position: Customer to retain original copy. Distributor must send copy to: R-Biopharm AG An der neuen Bergstraße 17 64297 Darmstadt, Germany...

  • Page 45: Appendix Iv - Autoblot- Decontamination Certificate

    RIDA qLine autoBlot Revision 1.0 | User manual ® 12.4 Appendix IV - autoBlot- Decontamination certificate Institute Name Make of Instrument Model Number Tick: This equipment has not been used in an invasive procedure or been in contact with blood, other body fluids or pathological samples.

  • Page 46: Assay Protocol

    RIDA qLine autoBlot Revision 1.0 | User manual ® 12.5 Assay protocol This test is a nitrocellulose membrane-based enzyme immunoassay (immunoblot assay) for the quantitative detection of allergen-specific IgE antibodies in human serum and plasma (citrat). Test procedure: The test procedure is designed for 36 membranes in the RIDA qLine autoBlot ®...

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