Changing dichroic filters:
Extra precaution is necessary when mounting or removing dichroic filters. Lay the dichroic filter holder flat on
a flat surface so that the mounting screws are facing upwards (see figure)
Using a Phillips screw driver unscrew the filter holding plate . Now gently remove the holding plate.
It is important how the dichroic mirror is mounted on the holder. The coated surface of the mirror should be
facing away from you as it sits on the flat surface. In other words the coated surface should face the light
source. The coated surface has the beveled edge or on occasion the manufacturer would specify using an
arrow marked at the edge.
Once the mirror is placed in the filter wheel, carefully place the holding plate in position and gently tighten
the screws. Make sure not to tighten it too much since this may cause the mirror to fracture. A good way to
keep check is to gently shake the filter wheels after a few turns on the screw, if you here a rattle you can
tighten a bit more. Follow this until the rattle stops.
The filter are now ready for mounting on the confocal head.
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