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Dynamica Velocity 15HR Instruction Manual

Refrigerated microcentrifuge
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Model Velocity 15HR High Speed Micro Centrifuge
Velocity 15HR
Micro Centrifuge
Instruction manual
Version: 1.0
Revision: D01-201802

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Summary of Contents for Dynamica Velocity 15HR

  • Page 1 Velocity 15HR Micro Centrifuge Instruction manual Model Velocity 15HR High Speed Micro Centrifuge Version: 1.0 Revision: D01-201802...
  • Page 3 Dynamica Velocity 15HR Refrigerated Microcentrifuge Copyright © 2018 Dynamica Scientific Ltd. All rights reserved. No part of this document may be reproduced or transmitted in any form or any means without permission from Dynamica Scientific Ltd. The names of actual companies and products mentioned herein may be the trademarks of their respective owners.

  • Page 4: Safety Reminder

    ・Do not operate this centrifuge in any manner not described in this Instruction Manual. Should you have any troubles with this centrifuge, call a Dynamica authorized sales/service representative. ・The precautions described in this Instruction Manual are carefully developed in an attempt to cover all the possible risks.
  • Page 5 Dynamica authorized sales/service representative. 3) It is your responsibility to sterilize and/or decontaminate the centrifuge, rotor, or parts properly before returning them to a Dynamica authorized sales/service representative.
  • Page 6 8. If the centrifuge will not be used for a long time, remove the power cord from the receptacle. 9. Depending on the magnitude, an earthquake might damage the centrifuge. If you observe some abnormality, contact a Dynamica authorized sales/service...

  • Page 7: Table Of Contents

    4.1 Setting the operating conditions 4.1.1 Setting the speed 4.1.2 Setting the RCF 4.1.3 Setting the running time 4.1.4 Setting the rotor temperature (Velocity 15HR) 4.1.5 Setting the acceleration and deceleration rate 4.2 Normal Operation 4.3 Programmed Operation 4.4 Spin-down Operation 4.5 Calculating Relative Centrifugal Force (RCF)
  • Page 8 APPENDIX Decontamination Sheet WEEE Compliance Marking for the restriction of the use of hazardous substances in electrical and electronic product (THE PEOPLE’S REPUBLIC OF CHINA) Rotor Guide Rotor Instruction Manual Warranty back cover After-sales Service back cover...

  • Page 9: Structure

    1. Structure 1.1 Location of the component Velocity 15HR Door Door Hook Drive Shaft Rotor Chamber Window of the door ＊ Operation Panel ■ Left side Drain hose Inlet POWER switch ＊You can measure the rotor speed through the window of the door by a optical...

  • Page 10: Operation Panel

    1. Structure 1.2 Operation panel Velocity 15HR ④ SPEED/RCF setting button ② Deceleration rate ③ SPEED/RCF display indicator ⑮ PULSE button ⑯ TEMP display ①Acceleration rate indicator ⑤ TIME display ⑭ OPEN button ⑪ DISP/CE button ⑨ ACCEL button ⑧ ♪ button ⑫...
  • Page 11 1. Structure Name Function Acceleration rate Indicates the rate of acceleration from the stop to 1,000 rpm by two ① indicator LEDs. Deceleration rate Indicates the rate of deceleration from 1,000 rpm and coasting ② indicator deceleration state by three LEDs. (1) Displays a speed or RCF setting or status value.

  • Page 12: Standard Accessories

    1. Structure 1.3 Standard accessories ■The standard accessories of the Velocity 15HR centrifuge are listed in Table 1.3. Check that all the standard accessories are provided before installation. Table 1.3 Standard accessories for Velocity 15HR Item name Part No. Q’ty...

  • Page 13: Installation

    2. Installation CAUTION： ！ ●Before moving the centrifuge, the rotor must be removed. This section describes instructions that you need to follow when installing the centrifuge. The instructions ensure your safety and maximum performance. (1) Location  Position this centrifuge on a firm and level floor. Avoid installing it on a slippery floor, or on a floor that conveys vibrations (for example, from people’s footsteps).
  • Page 14 This instructions ensure your safety and the maximum performance. (2) Power source Velocity 15HR: 120 V instrument: 108-126 V AC, 15 A, 60 Hz 220-230 V instrument: 198-253 V AC, 10 A, 50 Hz ; 198-242 V AC, 10 A, 60 Hz ...

  • Page 15: Preparation

    ！ CAUTION： ● Only rotors listed in Table 3.1 can be used in the Velocity 15HR centrifuge. ● To use the rotor properly, read its instruction manual carefully. ● Do not run the centrifuge over the allowable maximum speeds of the rotor, adapters, tubes.

  • Page 16: Installing The Rotor

    ！ ●If any abnormality such as corrosion or crack is found, stop using the rotor and contact a Dynamica authorized sales/service representative. (2) Remove the rotor cover from the rotor and check that there is no foreign matter in tube cavities.

  • Page 17: Preparing Samples

    3. Preparation 3.3 Preparing samples Prepare the sample to be centrifuged. ！ WARNING： ● This centrifuge and the rotor are not explosion-proof. Never use explosive or flammable samples. ●There are restrictions on the usage of biological samples and radioactive substances that require biological isolation such as pathogens and recombinant DNA for safety purposes.
  • Page 18 3. Preparation Load the tubes into the tube cavities of the rotor.  Load the tubes which are balanced symmetrically in the step (2) into the tube cavities. Example of correct tube settings and incorrect tube settings Correct settings: rotor Tube Incorrect settings: CAUTION：...

  • Page 19: Operation

    ● When operating the rotor at high speed after turning on the POWER switch in the Velocity 15HR centrifuge, the temperature of the rotor chamber might increase temporarily. However something is not wrong with the Velocity 15HR.

  • Page 20: Setting The Speed

    4. Operation 4.1.1 Setting the speed ① Check whether LED (color:green) at the right of the SPEED/RCF display has been extinguished. When the LED has been extinguished, perform the step ③. ② When the LED described in step ① lights, shift the mode from the RCF indication to the speed indication by pressing the ▼...

  • Page 21: Setting The Rcf

    4. Operation 4.1.2 Setting the RCF ① Check whether LED (color:green) at the right of the SPEED/RCF display has been lit. When the LED has been lit, perform the step ③. ② When the LED described in step ① is extinguished, shift the mode from the speed indication to the RCF indication by pressing the ▼...

  • Page 22: Setting The Running Time

    4. Operation 4.1.3 Setting the running time NOTE： ●This centrifuge has two kinds of timers. One is “Normal timer mode”, and the other is “Actual run timer mode”. When you choose “Normal timer mode”, the run time is counted as soon as the rotor is accelerated. When you choose “Actual run timer mode”, the run time is counted as soon as the speed of the rotor reaches the setting speed.

  • Page 23: Setting The Acceleration And Deceleration Rate

    4. Operation 4.1.5 Setting the acceleration and deceleration rate You can select acceleration and deceleration curves tailored to your jobs from two acceleration stages (FAST, SLOW), three braked deceleration stages (FAST, SLOW, FREE (coasting deceleration)). Acceleration and deceleration control has the time from 0 to 1,000 rpm variable, excluding the coasting deceleration.

  • Page 24: Normal Operation

    ● As soon as the power switch is turned on, the function of self-checking works. Any button does not work during self-checking. Velocity 15HR (the right side of the centrifuge) (2) Open the door and then set tubes into the rotor (see Chapter 3).
  • Page 25 ■In the Velocity 15HR centrifuge, turn off the POWER switch with its door open to dry the rotor chamber at the end of daily operation. If the Velocity 15HR centrifuge will not be used for a long time, turn off the POWER switch with its door open as well as the above.

  • Page 26: Programmed Operation

    4. Operation 4.3 Programmed Operation This centrifuge is featured with programmed operation that is able to store three running conditions in its memory and recall the condition by simply pressing a button. By using this feature, you are able to save a step for setting conditions. (1) Programming run conditions ①Turn on the POWER switch and set a desired condition to be programmed according to section 4.1.

  • Page 27: Spin-Down Operation

    ⑤Release the PULSE button. The rotor starts decelerating by releasing the button. Upon halt of the rotor, the ■ door lock is released automatically in the Velocity 15HR centrifuge, and the door is opened to a few cm automatically. PULSE...

  • Page 28: Calculating Relative Centrifugal Force (Rcf)

    4. Operation 4.5 Calculating Relative Centrifugal Force (RCF) An RCF can be determined by calculation or by referring to the graph provided below. The rotating radius of each rotor is given in the rotor instruction manual. Calculation formula: RCF = 1,118 x Rotating radius (cm) x Rotating speed (rpm) x 10 Calculation graph is shown below.

  • Page 29: Other Functions

    5. Other Functions 5.1 Selecting Rotor Stop Signal The rotor stop signal can be selected from seven kinds of sounds including five tunes, electronic beep, and no sound. Currently selected sound starts as the ♪ button is pressed. Press again before the sound ends to move to the next sound and the last played sound is chosen as the rotor stop signal.

  • Page 30: Indicating The Total Run Hours

    5. Other Functions 5.2 Indicating the total run hours ■The total run hours can be indicated in this centrifuge. The total run hours can be displayed on the operation panel. Inspect the centrifuge periodically by referring it. ■The total run hours of the centrifuge includes the time for which the rotor reaches the set speed whether you select the actual run timer or not.

  • Page 31: Actual Run Timer

    5. Other Functions 5.3 Actual run timer ■This centrifuge has two kinds of timers. One is normal timer and the other is actual run timer. The time for which the rotor reaches the set speed varies depending on rotor types or your samples. Actual run timer is effective to get stable separation of your samples because as soon as the rotor reaches the set speed the actual run timer works.

  • Page 32: Rotor Condition Indicator

    5. Other Functions 5.4 Rotor condition indicator ■When you choose the rotor condition indicator, you can see easily that the rotor is rotating at the set speed. After the rotor reaches the set speed, the run time and the rotor condition indicator are displayed by turns. ex.) When run time is 10 minutes LED for the TIME display follows the arrows.

  • Page 33: Troubleshooting

    ！ ● Unspecified repairs, remodeling or disassembly of the centrifuge that is not listed below is strictly prohibited other than a Dynamica authorized service representative. ● Close the door immediately if the rotor is still rotating when the door is opened after releasing the alarm status.
  • Page 34 ■ Alarms E-1 to 9 are mainly related to handling errors. You can continue using the centrifuge if the cause is removed. If alarm codes can not be cleared by taking the proper action, contact a Dynamica authorized sales/service representative.

  • Page 35: Troubleshooting When No Alarm Code Is Displayed

    POWER is turned blown out. If alarm codes can not be cleared by taking the proper action, contact a Dynamica authorized sales/service representative. Building power Power is wired from a single The power capacity may be exceeded.

  • Page 36: Opening The Door During Power Outage

    If the door cannot be opened due to a power outage, open the door according to the following steps. (Below are the illustrations about the Velocity 15HR centrifuge.) (1) Check that the rotor is not rotating.
  • Page 37 6. Troubleshooting (3) Insert the attached tool for setting the rotor horizontally into the hole for unlocking the door to fit the tool for setting rotor into the shaft inside the centrifuge. NOTE： ●You can not see the shaft for unlocking the door, because it is inside the centrifuge. Insert the attached tool for setting the rotor horizontally until the tool contacts the shaft.

  • Page 38: Maintenance

    ● Using cleaning or sterilization methods other than recommended in this instruction manual may cause in corrosion or deterioration of this centrifuge. Refer to chemical resistance chart attached to the rotor or contact Dynamica ● Unplug the main power cord before cleaning or sterilizing the instrument.

  • Page 39: Defrosting（Velocity 15Hr

    7. Maintenance 7.2 Defrosting（Velocity 15HR） When the rotor chamber is frosted, the cooling ability might be degraded. Defrost the rotor chamber by performing the following procedures. (1) Open the door of the centrifuge and turn off the POWER switch. (2) Hold up the left side of the centrifuge a little. Then remove two clamps on the bottom of the centrifuge and pull the drain hose.

  • Page 40: Tubes And Bottles

    7. Maintenance 7.3 Tubes and bottles (1) Cleaning and sterilizing tubes and bottles Use the best method for cleaning and sterilizing tubes and bottles referring to the table below. Cleaning and sterilizing conditions for tubes and bottles ○: Applicable ×: Inapplicable Material Condition Acidic (pH5 or lower)
  • Page 41 7. Maintenance (4) Conditions and life expectancy of tubes and bottles ！ CAUTION： ● Do not use tubes/bottles that have exceeded their life expectancy. Failure to do so could result in damage of tubes/bottles and the rotor and the centrifuge. The life expectancy of tubes/bottles depends on factors such as the characteristics of samples, speed of the rotor used, and temperature.

  • Page 42: Service Decontamination Policy

    Dynamica may ask you about the treatment for the centrifuge, rotor or the part if the decontamination is checked and judged as insufficient by Dynamica. It is your responsibility to bear the cost of sterilization or decontamination.

  • Page 43: Specifications

    *1: Because the instrument labels may differ from one country to another, please contact your local dealer or a Dynamica authorized sales/service representative. The Velocity 15HR centrifuges satisfy CE marking requirements. The CE marking is an international symbol, which shows that the product conforms to EU directives.
  • Page 44 Be sure to decontaminate the product according to good laboratory procedures and methods, and fill out this Decontamination Sheet and attach it to the product to be returned to Dynamica for repair. Attention: Dynamica Scientific Ltd. Decontamination Sheet Date ：...
  • Page 45 For further information regarding return, collection, recycle or disposal, please contact your local dealer or a Dynamica authorized sales/service representative. Marking for the restriction of the use of hazardous substances in electrical and electronic product (THE PEOPLE’S REPUBLIC OF CHINA)
  • Page 46 Names and Contents of Hazardous Substances in Each Component of This Centrifuge (Model of centrifuge: Velocity 15HR Hazardous Substances Cr(VI) PBDE Name of Component       Printed wiring board Ass’y      ...
  • Page 47 ROTOR GUIDE Rotor Factory Part number Specification rotor code FAS15E T15A61 D3-90591900 Max Speed: 15,000 rpm, Max RCF: 21,500 x g Tube Capacity: 1.5/2ml x 24 With plastic cover FAS15F T15A62 D3-90592000 Max Speed: 15,000 rpm, Max RCF: 21,500 x g Tube Capacity: 1.5/2ml x 24 With metal cover FAS15G...
  • Page 48 Cross-sectional view of rotor 2018.05 Dynamica Scientific Ltd Copyright © 2018 Dynamica Scientific Ltd. S99994105D All rights reserved. No part of this document may be reproduced or transmitted in any form or any means without permission from Dynamica Scientific Limited...
  • Page 49 ・This rotor can separate sample whose mean density is 1.2ｇ/mL or less. When the mean density of the sample to be separated is over 1.2ｇ/mL, calculate the allowable speed according to the following equation. （ｇ/ｍL） Allowable speed (rpm)＝ 15,000(rpm)× 試料の平均密度（ｇ/ｍｌ） Mean density of sample ( g /mL) ・To find the RCF: Substitute the rotation radius (Rmax., Rmin., etc.) and the speed in the following equation.
  • Page 50 FAS15F 0.5mL tubes can be used without adapters in this rotor. Adapter Tube Max. speed Nominal Actual (Max. RCF) Part No. Name Part No. Name Size (mm) capacity capacity (Marketed) 2.0 mL 1.7 mL (D) 10.8×(L) 39 2.0 mL micro tube ＊...
  • Page 51 FAS15G Single or 8-strip tubes and caps can be used in this rotor. Adapter Tube Max. speed Nominal Actual (Max. RCF) Part No. Name Name Size (mm) Part No capacity capacity (Marketed) 0.2 mL (D) 6×(L) 24 0.2 mL PCR tube (single) ＊...
  • Page 52 ・ When using tubes on the market, perform operation under the allowable speed or the allowable RCF specified by the manufacturer. Otherwise the tubes may be broken during operation. ！ CAUTION ： When using tubes outside 4-25 ℃ , tubes may be broken or deformed during operation. Test them before actual operation and perform actual operation after checking that there is no inconveniences such as the above.
  • Page 53 Operating Procedure 1. Install the rotor according to the procedures specified in the centrifuge instruction manual. ！ CAUTION ： Be sure to tighten the nut with the attached tool for setting the rotor. Be sure to use the attached tool to tighten the nut. Otherwise it might be difficult to remove the tool from the nut. If you tighten the nut insufficiently, the rotor might abnormally vibrate or come off.
  • Page 54 5. Load the balanced tubes and adapters in the rotor symmetrically with respect to the center of rotation of the rotor . ！ CAUTION ： Do not load the tubes asymmetrically. Asymmetrical loading may cause imbalance operation and the centrifuge may be damaged. Examples of incorrect tube setting are shown below.
  • Page 55 ＊ Although the contact mark may remain on the rotor when you use the rotor cover (Polypropylene) repeatedly, there is no problem. Contact part with the cover-locking knob Mounting the rotor cover (AL) on FAS15E/15F/15G rotor Cover-locking knob O-ring (1) Apply silicone grease (vacuum grease) (Part Rotor cover No.483719) to the rotor packing (O-ring)and then fit the rotor packing in the groove of the cover.
  • Page 56 Maintenance ! WARNING ：● If the rotor or an accessory is contaminated by samples that are toxic or radioactive, or blood samples that are pathogenic or infectious, be sure to decontaminate the item according to good laboratory procedures and methods. If there is a possibility that the rotor or an accessory is contaminated by samples that might impair human health (for example, samples that are toxic or radioactive, or blood samples that are pathogenic or infectious), it is your responsibility to sterilize or...
  • Page 57 Note: This warranty covers the rotor only, and Dynamica shall not be liable for damage to samples or other items that may arise from failure of or damage to the rotor. 2. Warranty Period This warranty is valid for seven years from the date of delivery of the rotor by Dynamica or a Dynamica dealer. 3. Notes (1) This warranty extends only to the original buyer from Dynamica or the original buyer from a Dynamica dealer.
  • Page 58 MEMO...
  • Page 59 Failures caused by conveyance or relocation after installation （4） Failures caused by modification or disassembly without Dynamica’s permission （5） Failures caused by use of rotors, buckets, adapters, tubes and bottles that are not （6）...
  • Page 60 The Velocity Range Bench Top Centrifuges Dynamica Scientific Limited 2 Bain Square, Kirkton Campus, Livingston, EH54 7DQ, United Kingdom P: +44 1908 211 900 F: +44 1908 211 909 Email: info@dynamica-eu.com Web: www.dynamica-eu.com Australasia Dynamica Pty Ltd ABN 16 126 043 052...

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