General Cleaning; Preventive Maintenance; Monitoring Temperature - CHART MVE SC 2/1V Operating Manual

damage that may jeopardize its functionality. Replace any aged/worn-out impact absorption foam
cushion inside the PPSC or any defective hardware. Remove badly damaged PPSCs from service.
Contact Customer or Technical Service for assistance in selecting the proper service parts.
Typical PPSC shown. The Shock absorbing foam in the
shape of the PPSC helps PPSC helps prevent damage to
keep the product upright the dewar. Illustration shows top
during shipment. view of the tank in the PPSC.
Select the appropriate shipping method based on the value of contents. Common carriers handle
packages very roughly with the most stable side of the packaging down, and should be used for
low-value product shipment only. Contact carriers for shipment methods for their recommendation
on transporting valuable and/or irreplaceable contents.
MVE Vapor Shippers were primarily designed as vapor shipping containers, however, they can also
be used for immersion of samples. It is imperative that all liquid nitrogen be removed so the unit
remains classified as a Vapor Shipper for transport. If liquid nitrogen is visible in the bottom of the
inner, it then becomes a liquid shipper and the exception status is void. The liquid inside is now
classified as hazardous material.

GENERAL CLEANING

Do not use any petroleum based cleaning solution.
Inside of the vessel:
Any cleaning solution that does not react with aluminum, stainless steel, or G10 composite can be used
in the sanitation process of a MVE Vapor Shipper. In most cases, any household detergent or mild soap
solution is suitable. Other cleaners and disinfectants that can be used safely include hydrogen peroxide,
chlorine/water mixture, and denatured alcohol. The generally accepted practice of using 10% chlorine
bleach with 90% water solution holds as the best method for decontamination. It is recommended that
the unit is filled to its full capacity with the cleaning solution mixture, agitated, and then thoroughly
rinsed. It is important that all surfaces being sanitized are thoroughly rinsed and that all cleaner solution
residues are removed after cleaning. Allow the unit to dry completely before putting into service. It is
suggested that the unit is inverted to drain and dry completely.
Outside of the vessel:
Use light dampened cloth of mild soap solution.

PREVENTIVE MAINTENANCE

The owner may follow two methods of maintenance, Continuous and/or Annual.
For Continuous Maintenance (Recommended before each shipment):
If the tank is warm follow steps 1 - 3 under OPERATION section above but allow the unit with full
liquid to stand undisturbed for 2 hours. Verify no major frost or condensation on outside of unit.
Frost or condensation on the outside of unit would indicate either a weak or no vacuum. Inspect the
cork and cover for signs of damage that may affect NER and Hold Time. Inspect the outside of the
tank for signs of shipping damage, such as large dents, especially around the neck tube area.
Remove badly damaged tanks from service.
The rubber latch design allows
the use of a zip tie, vet seal,
safety seal, etc. to ensure
integrity of product shipped.
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Clean up any stain, contamination or condensation on the bottom of the unit after dumping liquid
out. Keep the unit in a cool, clean, and dry location for storage. These procedures can help prevent
corrosion on the bottom of the Vapor Shipper.
For Annual Maintenance:
1. Weigh empty unit with cork/cover but without any inventory accessories and record as Empty
Weight, [lb].
Empty Weight must be taken before filling, while the inside of the Vapor Shipper is at room
temperature.
2. Fill unit to bottom of neck tube. Refer to OPERATION section step 2 for details.
3. Replace cork/cover and allow unit to stand for minimal 24 hours.
4. Weigh unit and record as First Weight, [lb].
5. Allow filled unit to sit undisturbed for another 24 (+/-0.25) hours.
a. Consider the accuracy and resolution of your scale to determine if additional days are
required between the first and second weights in order to obtain an accurate NER. Make sure
to record number of hours between first and second weight.
6. Weigh second time and record as Second Weight, [lb].
7. Calculate evaporation rate by using Equation 2. The difference between the first weight and the
second weight is the daily evaporation rate in lbs. This figure roughly signifies the normal
evaporation rate, or N.E.R, [Liter/Day]
(First Weight – Second Weight) x 13.468
NER =
Number of Hours
If there is major frost or condensation on outside of container during this time, it would
indicate either a weak or no vacuum.
8. Pour out LN2 per OPERATION section step 4. Replace cork/cover and take weight as Charged
Weight.
9. Calculate Hold Time, [Days], by using Equation 3. Subtract the Empty Weight from the Charged
Weight and divide by 1.782; then divide by NER.
(Charged Weight – Empty Weight) ÷ 1.782
Hold Time =
NER

MONITORING TEMPERATURE

MVE supplies Data Loggers for peace of mind when shipping valuable inventory in Vapor Shippers.
Contact Customer or Technical Service for assistance in selecting the proper Data Logger for
your product. The datalogger adds approximately 0.12L/day NER.
LETTER EXPLAINING EXCEPTION
This is concerning the applicability of the USA Federal Hazardous Material Regulations to the shipment of
refrigerated samples in the "Dry Shipper" container. A "Dry Shipper" package consists of an outer container that is
lined with an absorbent material. The container is charged with nitrogen refrigerated liquid which is absorbed into
the container lining. The charged, completed package serves as refrigerated container for the shipment of samples.
In consideration of the above, consultation with the Research and Special Programs Administration of the DOT
has determined that the use of nitrogen refrigerated liquid charged "dry shipper" containers for the shipment of
samples fall within the regulation exception provided in 49CFR 173.320 paragraph (a) of the section states the
requirements of this subchapter do not apply to atmospheric gases and helium when used in the operation of the
process system such as refrigeration system. Paragraph (c) of 173.320 pertains to air transport of same
refrigeration system. For exception status of air shipments please refer to IATA-Dangerous Goods Regulations for
nitrogen refrigerated liquid. This falls in the class of 2.2 nonflammable gas, packing instructions 202 with special
provisions A-152. If shipping outside of the USA, please consult your applicable regulatory agencies. For answers
to questions regarding shipping regulations contact a Chart, AI-Cryobiological Tech Service Representative.
Equation 2
Equation 3
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