Siemens Hematek Operating Manual page 70

Volume is lowest when a knob is turned fully counterclockwise and highest when
turned fully clockwise.
Volume increments are marked on each knob:
• Stain and buffer knobs: Each evenly labeled increment changes the volume by
• Rinse knob: Each evenly labeled increment changes the volume by approximately
2. Prime the instrument by placing the operating lever in the
it there until solution is being pumped at all three stations (stain, buffer, and rinse).
3. Wipe the platen after priming, wiping from right to left only.
4. Prime the platen by loading five priming slides and allowing them to be transported
across the platen.
This procedure primes and wets the platen to achieve proper surface tension
characteristics.
5. Adjust the stain volume as follows:
a. Run five blood smear slides across the platen immediately following the priming
b. Adjust the stain control knob so the stain just fills, but does not overfill, the
c. If the slide is receiving an inadequate amount of stain, adjust the control knob
d. If overflow of solution is visible on either side of the slide or an excess of stain is
The adjustment is correct when proper fill has been achieved on two consecutive
slides.
6. Adjust the buffer volume as follows:
a. If there has been a gap of more than two consecutive slides across the platen since
b. Run five blood smear slides across the platen immediately following the priming
When the slide passes the buffer section of the platen, mixing of the stain and buffer
occurs because of the mixing grooves. This is where the proper stain-to- buffer ratio is
most important. The slide looks under filled as it travels over the mixing grooves
because the capillary gap is slightly greater.
7. Adjust the buffer control knob so the stain-buffer mixture just fills the capillary space
under the slide when the slide is past the mixing grooves and onto the smooth portion
of the platen, just before the rinse area.
Ensure that the stain-buffer mix does not extend out from either side of the slide.
Page 6—16, Section 6: Minor Replacements and Adjustments
approximately 0.1 mL per 10 slides (0.01 mL/slide).
0.5 mL per 10 slides (0.05 mL/slide).
slides.
capillary space between the platen and the slide.
clockwise.
visible hanging over the front beveled edge of the platen, adjust the stain control
knob counterclockwise.
the stain volume adjustment, re-prime the platen with three priming slides.
slides.
position and holding
PRIME