Siemens ADVIA 2400 Maintenance Procedures
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Siemens ADVIA 2400 Maintenance Procedures

Siemens ADVIA 2400 Maintenance Procedures

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  • Page 3 5 Maintenance ......................109 Maintenance schedule..........................109 Daily maintenance........................... 110 Inspecting and cleaning the probes ........................110 Cleaning the probes using automatic advance probe motion................110 Cleaning the probes using manual probe motion ....................111 Probe cleaning procedure (optional method) ....................112 Inspecting and cleaning the mixing rods and mixer wash cups ................113 Checking reagents and system solutions.......................114 Inspecting and cleaning the reaction (WUD) and dilution (DWUD) cuvette washers ..........114 Inspecting and cleaning the cuvette splash covers....................117...
  • Page 4 Preparing the system for an expected power outage ..................171 Preparing the system for power return (if power was unexpectedly lost while system was on)......172 Recovering from an unexpected power outage (after power returns, when power was unexpectedly lost while system was on) ..............................172 ADVIA 2400 Operator’s Guide...

  • Page 5: Maintenance

    CAUTION The maintenance procedures described in this To avoid reporting faulty data, deterioration document are to be performed ONLY by Siemens- of reproducibility, or damage to the ISE trained users. Some of these procedures require the module and its parts, follow the precautions...

  • Page 6: Daily Maintenance

    3.DPPLR sample probe 16.SPPLR Reagent probe 1 37.RPPLR-1 Reagent probe 2 49.RPPLR-2 3. Select the number of times necessary to move the probe to the accessible Move location, then select to close the probe window. Exit ADVIA 2400 Operator’s Guide...

  • Page 7: Cleaning The Probes Using Manual Probe Motion

    Probe Accessible Location Dilution probe (DPP) Over the sample tray (STT) OR over the ISE Sample probe (SPP) Over the dilution tray (DTT) Reagent probe 1 (RPP1) Over reagent tray 1 (RTT1) Reagent probe 2 (RPP2) Over reagent tray 2 (RTT2) 4.

  • Page 8: Probe Cleaning Procedure (Optional Method)

    User Maintenance 3. At the User Maintenance window, in the Position Probe for Routine Cleaning area, select Start , then select when prompted. 4. Wipe the probe with prep pads or lint-free towels and 5% bleach solution. ADVIA 2400 Operator’s Guide...

  • Page 9: Inspecting And Cleaning The Mixing Rods And Mixer Wash Cups

    5. Check the alignment of the probe to the cuvette. If the probe is not centered over the cuvette, call your local technical support provider or distributor. 6. Close the User Maintenance window, then select when prompted. 7. At the Operation Panel, select to return all probes to the home position Initialize (over the wash cups).

  • Page 10: Checking Reagents And System Solutions

    Perform this inspection in addition to the startup, shutdown, and weekly washes, to keep the WUD and DWUD from clogging. In the event of a clog, call your local technical support provider or distributor for assistance. ADVIA 2400 Operator’s Guide...
  • Page 11 Dilution Cuvette Wash Station (DWUD) Reaction Cuvette Wash Station (WUD) Figure 5-4. Cuvette wash stations 3. Remove the wash head: a. Cover nearby cuvettes with lint-free toweling to protect them from dust. b. Loosen the retaining screw ( ) with a 4-mm hex wrench. c.
  • Page 12 ., then select Exit to raise the washer nozzles. 9. At the Operation Panel, select Initialize , then verify the DWUD and the WUD are in the up position and the instrument is in the READY state. ADVIA 2400 Operator’s Guide...

  • Page 13: Inspecting And Cleaning The Cuvette Splash Covers

    Inspecting and cleaning the cuvette splash covers Materials required: • Lint-free towel BIOHAZARD • Deionized water Wear personal protective equipment. Use universal precautions. Time: 5 minutes Analyzer mode: READY NOTE Cuvette covers are installed around the probes to prevent water and reagent from entering the dilution and reaction cuvettes.

  • Page 14: Inspecting And Cleaning The Probe Wash Cups

    Dry the sensor. To clear the alarm message, on the Operation Panel, select the alarm icon. 5. At the Operation Panel, select Initialize to return the system to READY mode. ADVIA 2400 Operator’s Guide...

  • Page 15: Checking Pumps For Leaks

    Checking pumps for leaks A decrease in liquid flow or the presence of air bubbles in the lines may be due to a leaking pump. Inspect the pumps for leaks daily to identify potential problems. Materials required: • Lint-free towel BIOHAZARD Time: 10 minutes Wear personal protective equipment.

  • Page 16: Checking Other Vertical Pumps For Leaks

    DWP4; the reaction cuvette wash pumps, WP1, WP2, and WP3; and reaction cuvette detergent pumps DTP1 and DTP2). 1 DWP1 5 WCV2 9 WP3 2 DWP2 6 WCV1 10 DTP1 3 DWP3 7 WP1 11 DTP2 4 DWP4 8 WP2 Figure 5-12. Horizontal pumps ADVIA 2400 Operator’s Guide...

  • Page 17: Performing The Startup Wash (Wash3)

    There are two types of horizontal pumps, those with double seals and those with a single seal. The method of checking for leaks is the same for both: 1. To determine if any of these pumps are leaking, inspect the front portion ( Figure 5-13.
  • Page 18 By choosing CTT position #50 for WASH 2 and CTT position #51 for WASH 3, you only need to refill the CTT container once. 3. Ensure the container at RTT1 ( ) and RTT2 ( ) position #50 contains DI water. ADVIA 2400 Operator’s Guide...

  • Page 19: Performing The Shutdown Wash

    NOTE At your laboratory’s discretion, you may use other positions for the washes on each of the trays, but you must change the entries for the alternate positions in the appropriate fields on the WASH Set window. 4. At the WASH Set window, define the WASH3 container positions as follows: a.

  • Page 20: Performing Additional Ise Electrode Washes

    Recording ISE slopes Once a day, record the slopes from a successful ISE calibration on the Maintenance Log. The slopes are provided on the ISE Monitor, RBL/Calibration History, and RealTime Monitor windows following a successful calibration. ADVIA 2400 Operator’s Guide...

  • Page 21: Weekly Maintenance

    Weekly maintenance Performing the weekly wash Materials required: • 5% solution of Reagent Probe Wash 3 BIOHAZARD (REF 03164495, PN B01-4183-01) Wear personal protective equipment. • ISE Wash (REF 01307361, Use universal precautions. PN B01-4174-01) • Deionized water Time: 38 minutes Analyzer mode: READY Laboratories running the system more than 8 hours per day or running large numbers of dialysis or urine samples are advised to perform this Weekly wash procedure...

  • Page 22: Checking And Replenishing The Lamp Coolant

    3. If the reservoir fluid level is less than 5 cm, add coolant as follows: a. Turn the reservoir cover counterclockwise to remove it. b. Fill the reservoir to the 9-cm mark with a 5% solution of Lamp Coolant Additive (REF 04533710, PN B01-4496-51) in deionized water. ADVIA 2400 Operator’s Guide...

  • Page 23: Checking Lamp Energy

    1 Upper Mark (Green Line) 2 Lower Mark (Red Line) Figure 5-15. Reservoir levels Upper and Lower c. Replace the reservoir cover. Do not over tighten. 4. Replace the lamp access cover. NOTE If adding coolant does not clear the lamp coolant warning, call your local technical support provider or distributor.
  • Page 24 Enter c. Subtract 50 from the 340-nm AD count ( ) and type the noted in step 10 a difference in the bottom field, to the left of the graph, then press Enter ADVIA 2400 Operator’s Guide...

  • Page 25: Reading Lamp Energy Data

    The lamp energy displays as a scatter plot. • If the points are mostly within ±40 of the center, the lamp is normal. • Otherwise replace the lamp Only if you replaced the lamp or a cuvette segment, select Regist Data , then select in the Registration window.
  • Page 26 • Abnormal cuvettes remain registered as abnormal until a future measurement determines that they can be used. Reference value The reference value (the average value of the measurements of all cuvettes) remains the same until the next measurement. ADVIA 2400 Operator’s Guide...

  • Page 27: Cleaning The Analyzer And Rack Handler Exterior Panels

    Cleaning the analyzer and rack handler exterior panels Materials required: • Lint-free towel BIOHAZARD • 10% solution of bleach (5% sodium Wear personal protective equipment. hypochlorite) and water Use universal precautions. Time: 10 minutes Analyzer mode: READY 1. Put the analyzer and rack handler (if applicable) in Standby mode. 2.

  • Page 28: Monthly Maintenance

    2 STT Tray 3 CTT Nylatch Fasteners - 2 places 4 CTT Handle 5 STT Nylatch Fasteners - 2 places 6 STT Handles - 2 places 7 Locator Screw Figure 5-16. Components of CTT and STT trays ADVIA 2400 Operator’s Guide...

  • Page 29: Cleaning The Inside Of The Reagent Tray Refrigerated Housing

    4. Replace the CTT and STT tray and covers. a. Orient each tray loader to the locator screw ( b. Ensure the tray loaders are securely in position, then push the fasteners ( in place. c. Replace the STT evaporation cover. d.

  • Page 30: Cuvette Wash And Cuvette Conditioner Usage

    • Physiological saline (0.9% NaCl) Wear personal protective equipment. Use universal precautions. Time: 10 minutes Analyzer mode: READY NOTE The dilution bottle may be cleaned when it is refilled, but must be cleaned at least once a month. ADVIA 2400 Operator’s Guide...

  • Page 31: Cleaning And Replenishing The Cuvette Wash Bottle

    1 ISE buffer bottle 2 RRV (Reaction) bath oil 3 Isotonic saline diluent bottle 4 Cuvette detergent bottle 5 Cell conditioner bottle Figure 5-17. Isotonic Saline diluent bottle 1. Lift the cover from the saline diluent bottle ( ), and remove the bottle. CAUTION Note the bottle position on the shelf, to avoid mixing up the fluid bottles.
  • Page 32 8. Connect the cuvette wash bottle level sensor connector, then push the connector in and turn it clockwise. 9. Insert the filter and hose, then fasten the cap. NOTE Make sure that the filter holder is located at the bottom of the bottle. 10. Prime the fluid lines: NOTE ADVIA 2400 Operator’s Guide...

  • Page 33: Cleaning The Chiller Filter

    If you are cleaning other bottles, wait to perform this step for all fluid lines. a. At the Operation Panel, select the button. Prime b. At the PRIME Settings dialog box, select Prime 2 and then type or more for the number of times in all fields.

  • Page 34: Every 2 Months Maintenance

    4. Wash the cuvettes under running water, then rinse them in deionized water. 5. Drain the water from the cuvettes. 6. Install the cuvette segments on the dilution tray (DTT) and fasten the thumbscrews by hand. 7. At the Operation Panel, select Initialize ADVIA 2400 Operator’s Guide...

  • Page 35: Cleaning And Replenishing The Cuvette Conditioner Bottle

    NOTE Verify that the Operating mode field displays READY before performing any further actions. 8. At the Operation Panel, select WASH 9. Perform the daily shutdown wash routine, then verify the operation. Cleaning and replenishing the cuvette conditioner bottle Materials required: •...
  • Page 36 If you are cleaning other bottles, wait to perform this step for all fluid lines. a. At the Operation Panel, select Prime b. At the Prime Settings dialog box, select and type for the Number of Prime 2 times in all fields. c. Select Execute ADVIA 2400 Operator’s Guide...

  • Page 37: Every 3 Months Maintenance

    Every 3 months maintenance Replacing the lamp Materials required: • Halogen lamp, 12 V/50 W BIOHAZARD (REF 02127928, PN 073-0099-01) Wear personal protective equipment. Time: 60 minutes Use universal precautions. Analyzer mode: OFF You must replace the lamp under the following conditions: •...
  • Page 38 11. Check the lamp energy (see the Weekly Maintenance section). 12. Perform the cell blank measurement test. NOTE Siemens recommends the assays on the system be calibrated after the lamp is replaced. 13. Run controls to verify that all assays are within the laboratory’s established control ranges.

  • Page 39: Washing The Ise Electrodes Lines

    Washing the ISE electrodes lines Materials required: • Dummy electrode with o-ring and cap BIOHAZARD (REF 05938765, PN 073-0342-01) Wear personal protective equipment. • ISE detergent solution Use universal precautions. (REF 01307361, B01-4174-01) Time: 25 minutes Analyzer mode: Manual operation 1.
  • Page 40 10. Verify no leaks or bubbles exist and that the buffer is going to the waste during the priming cycle. 11. At the ISE Operation window, next to the Initialize button, select Execute 12. Perform calibration and run controls. ADVIA 2400 Operator’s Guide...

  • Page 41: Every 4 Months Maintenance

    Every 4 months maintenance Cleaning the ancillary reagent bottle filters Materials required: • Filters (REF 08602474, PN 073-0033-01) BIOHAZARD order one set per bottle Wear personal protective equipment. Time: 20 minutes Use universal precautions. Analyzer mode: OFF Use this procedure to clean the filters in the following bottles •...

  • Page 42: Cleaning The Pure-Water Bottle Filter

    1. Ensure that the instrument is in READY mode. 2. Remove the silicon return hose (1) from the top front of the pure water bottle. The filter is contained in a metal filter holder attached to the end of the return hose. ADVIA 2400 Operator’s Guide...
  • Page 43 Silicon Return Line Metal Filter Holder Figure 5-25. Pure-water bottle lines and filter 3. Using pliers if necessary, unfasten the filter holder from the end of the return hose and remove the filter. NOTE If the filter is ripped or damaged, replace it with a new filter (18R). WARNING Household bleach is 5% or 6% sodium hypochlorite.

  • Page 44: Replacing The Reaction And Dilution Cuvettes

    • 6 sets of dilution cuvettes (sample cell DTT, single cuvette set, REF 05049669, PN 073-0022-01) Time: 20 minutes Analyzer mode: OFF Replace the 20 sets of reaction (RRV) cuvettes and 6 sets of dilution (DTT) cuvettes once every four months. ADVIA 2400 Operator’s Guide...
  • Page 45 1 Thumbscrew 2 Tab 3 Detector Unit Figure 5-27. Reaction and dilution cuvette components 1. Put the system in Standby Mode. WARNING Failure to put the analyzer into Standby mode will result in the RRV bath oil over-filling. Excess RRV bath oil can damage the spectrophotometer when the RRV cuvettes are reinstalled.
  • Page 46 8. Perform the daily Shutdown wash (WASH2) routine and verify the operation. 9. Perform the lamp energy check procedure. 10. Perform the cell blank measurement, and if the cell blank run was completed successfully, save the results. ADVIA 2400 Operator’s Guide...

  • Page 47: As Required Maintenance

    As required maintenance Backing up system files The system software consists of the operating system, data processing software, and user- specific system and data files. Back up the system files to a USB Memory Stick or a formatted CD / CD-RW / DVD disc whenever you make any configuration or parameter changes.

  • Page 48: Restoring System Files

    Time: 10 minutes Analyzer mode: STANDBY NOTE Use this procedure to replace SPP and RPP probes equipped with crash detection. For dilution probes (DPP) equipped with crash detection, refer to Replacing DPP probes - with crash detection. ADVIA 2400 Operator’s Guide...
  • Page 49 1 Dilution Probe (DPP) 2 Sample Probe (SPP) 3 Reagent Probe 1 (RPP1) 4 Reagent Probe 2 (RPP2) Figure 5-28. Probes Remove the probe 1. Put the system in Standby mode. CAUTION To keep from damaging the probe tip when the power is off, you must manually support the probe and be careful not to strike it against anything on the analyzer.
  • Page 50 9. Ensure that the probe is perpendicular to the arm and centered over the cuvette. If not, call your local technical support provider or distributor. 10. At the Operation Panel, select Initialize to return the probes back to home (over the wash cups). ADVIA 2400 Operator’s Guide...

  • Page 51: Replacing Dpp Probes Equipped With Crash Detection

    11. At the Operation Panel, select then select then to ensure PRIME PRIME 2 Execute proper water flow through the probe. NOTE Make sure that no water is leaking from the knurled fitting. Replacing DPP probes equipped with crash detection Materials required: •...
  • Page 52 ) from the post coming from the probe ( ), but leave the other blue wire attached. 13. Gently lift the probe ( ) up through the probe guide ( ), then carefully remove it from the probe arm ( ADVIA 2400 Operator’s Guide...
  • Page 53 14. Discard the old probe. Install the new probe 1. Carefully insert the new probe ( ) into the probe guide ( 2. Lower the probe fully into the guide so that the rear tube fitting rests in the joint holder ( 3.

  • Page 54: Replenishing The Rrv (Reaction) Bath Oil Bottle

    Make a note of the bottle position on the shelf, to avoid mixing up the fluid bottles. 3. Remove the RRV bath oil bottle ( CAUTION Ensure that level-sensor connector ( ) does not get wet, to avoid damaging it. ADVIA 2400 Operator’s Guide...

  • Page 55: Preventive Cleaning Of The Wash Station Lines

    4. Refill the bottle with RRV (reaction) bath oil. 5. Replace the bottle on the shelf in the cabinet. 6. Connect the RRV bath oil bottle level sensor connector ( ) by pushing the connector in and turning it clockwise. 7.
  • Page 56 Use a shallow tray for washing the nozzles. A tray with a depth of 35 – 40 mm is most suitable. Trays with higher sides may require additional wash solution. e. Place the dryer nozzle outside the tray (1) and all the other nozzles inside the tray. ADVIA 2400 Operator’s Guide...
  • Page 57 Figure 5-35. WUD and DWUD dryer nozzle Fill the tray with deionized water, being careful not to overflow the tray. g. Pour enough deionized water into the tray so that the center nozzles (yellow- labeled overflow nozzle) are in liquid. 3.
  • Page 58 22. Run a Startup Wash (WASH3) procedure on the system, then verify proper hydraulic operation and mechanical alignment of the WUD/DWUD assemblies during the Startup Wash. 23. Run your laboratory’s quality control material and verify the results are within acceptable ranges. ADVIA 2400 Operator’s Guide...

  • Page 59: Washing All The Ise Lines

    Washing all the ISE lines Materials required: • 2 clean, empty buffer bottles BIOHAZARD • Probe Wash 3 solution Wear personal protective equipment. Use universal precautions. • Dummy electrode • Phillips head screwdriver Time: 15 minutes Analyzer mode: Manual operation 1.

  • Page 60: Washing The Lines

    3. When the priming is finished, verify that the electrodes are not leaking. 4. At the ISE Operation window, select Exit , then select 5. Run 10 pooled serum samples, or do an ISE CV check. 6. Perform calibration and run controls. ADVIA 2400 Operator’s Guide...

  • Page 61: Conditioning The Ise Na And K Electrodes

    Conditioning the ISE Na and K electrodes Materials required: • 10 mL of serum pool BIOHAZARD • 30 mL of ISE Buffer Wear personal protective equipment. Use universal precautions. (REF 03463190, PN B01-4171-51) • 2-mL or 3-mL plastic, disposable pipette Time: 5 minutes (preparation) 24 hours (immersion) Analyzer mode: READY...

  • Page 62: Replacing Ise Electrodes

    • Reference (REF 00311764, PN 073-0653-01 • O-rings, 3 (REF 09955206, PN 073-0071-01) • Philips screwdriver Time: 5 minutes Analyzer mode: Manual operation Replace the Na, K, and the Cl electrodes if the slope is incorrect or calibration continuously fails. ADVIA 2400 Operator’s Guide...

  • Page 63: Removing Electrodes

    The acceptable ISE slope is between 45.0 and Mark ISE Slope Range 63.0. Slopes outside of this range are flagged > 65.0 as shown in the table. A flagged slope fails 63.1 to 65.0 the calibration. The slope limits are defined at 38.0 - 44.9 the ISE Parameter Settings window.

  • Page 64: Priming The Ises

    1. At the Operation Panel, select Initialize 2. At the Menu Panel, select ., then select Maint ISE Operation 3. At the ISE Operation window, in the Calibration area, select Execute 4. When prompted, select to execute calibration. ADVIA 2400 Operator’s Guide...

  • Page 65: Storing The Reference Electrode

    5. If the calibration fails, repeat calibration again and if data continues to be unstable, perform an electrode wash. NOTE The electrodes may have to stabilize on the system before a successful calibration is achieved. 6. At the ISE Operation window, select the button and enter the new Electrode Info electrode information.

  • Page 66: Cleaning The Waste-Drain Nozzle

    CAUTION Be careful not to scratch the nozzle. Damaging the nozzle may cause inaccurate results. 1. Using a blunt object such as a pipette, carefully scrape the crystals that are attached to the waste-drain nozzle ( ADVIA 2400 Operator’s Guide...

  • Page 67: Maintaining The Ise Unit After The Dilution Bowl And Waste-Drain Nozzle Are Clean

    Figure 5-40. Location of waste-drain nozzle 2. At the ISE Operation window, enter in the Bufferprime Times box, then select Execute IMPORTANT Verify that no buffer collects in the wash block. Buffer that remains in the wash block may clog the drain. Maintaining the ISE unit after the dilution bowl and waste-drain nozzle are clean 1.

  • Page 68: Preparing The System For Power Return (If Power Was Unexpectedly Lost While System Was On)

    Re-Start , then select 7. If possible, repeat the task prior to the power failure and verify that the data was stored. 8. If reagent was dispensed, you must perform a Weekly WASH2 before resuming operation. ADVIA 2400 Operator’s Guide...

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