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Table of Contents
Related Manuals for Cleaver Scientific multiSUB Midi96
Summary of Contents for Cleaver Scientific multiSUB Midi96
- Page 1 Midi96 Horizontal System...
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Page 3: Instruction Manual
multiSUB MIDI96 High ® throughput horizontal units Instruction Manual Catalogue Numbers MSMIDI96 MSMIDI96ST MSMIDI961.5 MSMIDI96ST1.5 MSMIDI96/2M MSMIDI96ST/2M MSMIDI96/1.5/2M MSMIDI96ST/1.5/2M Record the following for your records: Model _____________________ Catalogue No. _____________________ Date of Delivery _____________________ Warranty Period _____________________ Serial No. _____________________ Invoice No. -
Page 4: Table Of Contents
Contents Instruction Manual Catalogue Numbers Contents Safety Information Packing List Specifications Operating Instructions Usage Guidance and restrictions Setting up the Horizontal Gel Tanks Fitting Electrodes Fitting Loading Guides Gel Preparation Gel Pouring Casting Dams Flexicaster Tape Running the Gel Gel Staining and Viewing Solutions References Troubleshooting... -
Page 5: Safety Information
Safety Information When used correctly, these units pose no health risk. However, these units can deliver dangerous levels of electricity and are to be operated only by qualified personnel following the guidelines laid out in this instruction manual. Anyone intending to use this equipment should read the complete manual thoroughly. -
Page 6: Packing List
The unit should be checked for damage when received. Cleaver Scientific is liable for all missing or damaged parts / accessories within 7 days after customers have received this instrument package. Please contact Cleaver Scientific immediately regarding this issue. -
Page 7: Specifications
Specifications Power supply cables 1500V rated with retractable 4mm connectors Safety lid and viewing pane High impact moulded acrylic construction Height adjustable combs Acrylic UV transparent gel tray UV transparent above 300nm, Moulded acrylic Comb Slots Allow multiple combs per tray Casting dams Natural rubber Colour-coded electrodes... -
Page 8: Operating Instructions
Operating Instructions Further information (including videos) regarding setting up and running the multiSUB units can be found at www.cleaverscientific.com ® Usage Guidance and restrictions Maximum altitude 2,000m. Temperature range between 4°C and 65°C. Maximum relative humidity 80% for temperatures up to 31 decreasing linearly to 50% relative humidity at 40 ... -
Page 9: Gel Preparation
2. Remove the tray. 3. Peel the back off the loading guide and carefully apply the loading guide directly to the gel platform. The unit is now ready to be used. Gel Preparation The Table below shows the volume of agarose solution required to make the desired agarose gel for each unit tray size. -
Page 10: Gel Pouring
Gel Pouring The multiSUB range of units allows three different methods of gel casting: ® 1. Casting Dams 2. Flexicaster 3. Traditional Tape Casting Dams 1. To fit the casting dams, place one casting dam on the bench with the groove facing upwards (1). -
Page 11: Flexicaster
Flexicaster 1. Level the Flexicaster base by adjusting the feet so that the bubble is exactly central. 2. Insert the desired length tray into the Flexicaster such that one end of the tray is pushed up and seals against the silicone mat of the permanent end of the Flexicaster. -
Page 12: Tape
Tape 1. Autoclave or plastic backed general tape should be used. A length 5cm longer than the width of each end of the tray should be cut. One length should be placed over one end of the tray and stuck m1cm in from the tray edge. -
Page 13: Gel Staining And Viewing
Gel Staining and Viewing The Multi Sub trays allow staining to be performed without removing the gel from the tray if this is preferred. 1. Transfer the gel to a vessel containing the appropriate volume of 0.5 µg/ml ethidium bromide stain for 15–30 minutes, see solutions for stock stain concentration and adjust to the volume used accordingly. -
Page 14: References
10X TBE stock (1L) dissolve in 750 ml distilled water: 108 g tris base (FW = 121) 55 g boric acid (FW = 61.8) 40 ml 0.5 M EDTA (pH 8.0) Fill to 1 litre final volume with distilled water Loading Dye 10x sample buffer stock consists of 50% glycerol, 0.25% bromophenol blue, and 0.25% xylene cyanole FF in 1x TAE buffer. -
Page 15: Troubleshooting
Decrease agarose percentage. Bands sharp but not enough high Review enzyme activity, digest bands seen Incomplete digestion further. Consult Cleaver Scientific about agarose. Agarose has improper endosmosis Reduce salt concentration to ≤0.1M. Salt concentration in sample too Reduce voltage. See high electrophoresis instructions. -
Page 16: Care And Maintenance
Care and Maintenance Cleaning Horizontal Units Units are best cleaned using warm water and a mild detergent. Water at temperatures above 60°C can cause damage to the unit and components. The tank should be thoroughly rinsed with warm water or distilled water to prevent build-up of salts, but care should be taken not to damage the enclosed electrode and vigorous cleaning is not necessary or advised. -
Page 17: Ordering Information
Ordering information Combs Catalogue No. Description MSMIDI96-8-1-CB MSMIDI 96 Comb 8 sample MC + 1 Marker, 1mm thick COMB BLOCK MSMIDI96-8-1.5-CB MSMIDI 96 Comb 8 sample MC + 1 Marker, 1.5mm thick COMB BLOCK MSMIDI96-8-1/2M-CB MSMIDI 96 Comb 8 sample MC + 2 Marker, 1mm thick COMB BLOCK MSMIDI96-8-1.5/2M-CB MSMIDI 96 Comb 8 sample MC + 2 Marker, 1.5mm thick... -
Page 18: Related Products
Related Products Catalogue No. Product description nanoPAC-300 Mini Power supply, 300V, 400mA, 60W -100 -240VAC nanoPAC-500 Mini Power supply, 500V, 400mA, 120W -100 -240VAC POWERPRO300 MIDI Power Supply, 300V, 700mA, 150 – 100 -240VAC OMNIDOC plus Blue LED Epi-illumination Module (OMNIDOC-BL), and omniDOCPROSAFE 520, 560 &... -
Page 19: Warranty
Warranty The Cleaver Scientific Ltd. (CSL) multiSUB Horizontal Electrophoresis units ® have a warranty against manufacturing and material faults of twelve months from date of customer receipt. If any defects occur during this warranty period, CSL will repair or replace the defective parts free of charge.
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