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Cleaver Scientific MSMIDI96 Instruction Manual

Multi sub electrophoresis systems
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Instruction Manual
Multi Sub Electrophoresis Systems
MSMIDI96
MSMIDI96ST
MULTISUB4

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Summary of Contents for Cleaver Scientific MSMIDI96

  • Page 1 Instruction Manual Multi Sub Electrophoresis Systems MSMIDI96 MSMIDI96ST MULTISUB4...

  • Page 2: Table Of Contents

    Contents:- Page Safety Instructions Packing Lists Usage Guidance and restrictions Care and Maintenance Setting Up Gel Preparation Gel Pouring Gel Running Gel Staining Solutions Combs Warranty...

  • Page 3: Packing Lists

    SAFETY PRECAUTION WHEN USED CORRECTLY, THESE UNITS POSE NO HEALTH RISK. HOWEVER, THESE UNITS CAN DELIVER DANGEROUS LEVELS OF ELECTRICITY AND ARE TO BE OPERATED ONLY BY QUALIFIED PERSONNEL FOLLOWING THE GUIDELINES LAID OUT IN THIS INSTRUCTION MANUAL. ANYONE INTENDING TO USE THIS EQUIPMENT SHOULD READ THE COMPLETE MANUAL THOROUGHLY.
  • Page 4 MSMIDI96 Units include tank, lid and electrodes and include the following accessories:- Combs and Tray Tray Dams Loading Cables comb block Guides MS10-UV96 10 x MS10-UVDAM MS10-LG Strips MSMIDI96 MSMIDI96-8-1-CB CSL-CAB 12cm (W x L) Pack of 2 MS10-WP Platform...

  • Page 5: Usage Guidance And Restrictions

    Trays Combs Caster Cables 8 x MSUB4-18/8- 4 x MSUB4UV18 8 CSL-MULTISUB4 1.5 1.5mm, 18 and CSL-CAB x 18cm (W x L) 8 sample CSL- 8 x MSUB4-18/8- 1 x MSUB4EXCASTER 4 x MSUB4UV18 8 1.5 1.5mm, 18 and External Caster For Msub4 (4 CSL-CAB MULTISUB4EXCAS x 18cm (W x L)
  • Page 6 Maximum relative humidity 80% for temperatures up to 31 C decreasing linearly to 50% relative humidity at 40 Not for outdoor Use. This apparatus is rated POLLUTION DEGREE 2 in accordance with IEC 664. POLLUTION DEGREE 2, states that: Normally only non-conductive pollution occurs. Occasionally, however, a temporary conductivity caused by condensation must be expected .

  • Page 7: Care And Maintenance

    Care and Maintenance:- Cleaning Horizontal Units Units are best cleaned using warm water and a mild detergent. Water at temperatures above C can cause damage to the unit and components. The tank should be thoroughly rinsed with warm water or distilled water to prevent build up of salts but care should be taken not to damage the enclosed electrode and vigorous cleaning is not necessary or advised.

  • Page 8: Gel Preparation

    1. Note the position of the lid on the unit. This shows the correct polarity and the correct orientation of the cables, black is negative and red positive. 2. Remove the lid from the unit. Note if the lid is not removed, fitting the cables may result in un-tightening of the gold plug and damage to the electrode.
  • Page 9 Gel volume for a 5mm thick Gel volume for a 5mm thick Tray Gel volume for a 4x 5mm thick gels 60ml 120ml 8 x 6cm 96ml 8 x 12cm 192ml 8 x 18cm 288ml 2. Add the agarose powder to a conical flask. 3.

  • Page 10: Gel Pouring

    Gel Pouring:- The CSL Multi sub range of units allows three different methods of gel casting:- Casting Dams Flexicaster Traditional Tape Using trays with Casting Dams (Note for MultiSUB4):- 1. Fit the casting dams over each end of the tray and place onto a level surface. The dams should be fitted so that there is no gap between the sides of the tray and the groove in the dams.

  • Page 11: Gel Staining

    3. Pour in the agarose carefully so as not to generate bubbles. Any bubbles that do occur can be smoothed to the edge of the gel and dispersed using a pipette tip. 4. Allow the agarose to set, ensuring that the gel remains undisturbed. 5.

  • Page 12: Solutions

    NOTE:- Ethidium bromide is a suspected carcinogen and the necessary safety precautions should be undertaken. 2. De-stain the gel for 10 30 minutes in distilled water again ensuring the gel is completely immersed. 3. Rinse the gel twice for a couple of seconds with distilled water. 4.

  • Page 13: Combs

    Combs MC Denotes Multi Channel Pipette compatible. Multi Sub Midi 96 Combs Description Part Number MSMIDI 96 Comb 8 sample MC + 1 Marker, 1mm thick MSMIDI96-8-1-CB COMB BLOCK MSMIDI 96 Comb 8 sample MC + 1 Marker, 1.5mm MSMIDI96-8-1.5-CB thick COMB BLOCK...
  • Page 14 1mm thick COMB BLOCK MSMIDI 96 STRETCH Comb 8 sample MC + 2 Marker, MSMIDI96ST-8-1.5/2M-CB 1.5mm thick COMB BLOCK MSMIDI 96 Comb 8 sample MC, 1mm thick. one marker MSMIDI96-8-1 lane. MSMIDI 96 Comb 8 sample MC, 1.5mm thick. one marker MSMIDI96-8-1.5 lane.
  • Page 15 Notes...

  • Page 16: Warranty

    Warranty The Cleaver Scientific Ltd. (CSL) Multi Sub Horizontal Electrophoresis units have a warranty against manufacturing and material faults of twelve months from date of customer receipt. If any defects occur during this warranty period, CSL will repair or replace the defective parts free of charge.

This manual is also suitable for:

Msmidi96stMultisub4

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