multiSUB Screen ® Horizontal Electrophoresis Unit Instruction Manual Catalogue Numbers MSSCREEN16 MSSCREEN16-NC MSSCREEN24 MSSCREEN24-NC MSSCREEN32 MSSCREEN32-NC MSSCREEN-TRIO MSSCREEN-TRIO-NC Record the following for your records: Model _____________________ Catalogue No. _____________________ Date of Delivery _____________________ Warranty Period _____________________ Serial No. _____________________ Invoice No. _____________________ Purchase Order No.
Contents Instruction Manual Catalogue Numbers Contents Safety Information Packing List Specifications Operating Instructions Usage Guidance and restrictions Setting up the Horizontal Gel Tanks Fitting Electrodes Fitting Loading Guides Gel Preparation Gel Pouring Flexicaster Tape Running the Gel Gel Staining and Viewing Solutions References Troubleshooting...
Safety Information When used correctly, these units pose no health risk. However, these units can deliver dangerous levels of electricity and are to be operated only by qualified personnel following the guidelines laid out in this instruction manual. Anyone intending to use this equipment should read the complete manual thoroughly.
The unit should be checked for damage when received. Cleaver Scientific is liable for all missing or damaged parts / accessories within 7 days after customers have received this instrument package. Please contact...
Cleaver Scientific immediately regarding this issue. If no response within such period is received from the customer, Cleaver Scientific will no longer be liable for replacement/damaged parts. Please contact your supplier if there are any problems or missing items. Specifications...
Operating Instructions Further information (including videos) regarding setting up and running the multiSUB units can be found at www.cleaverscientific.com ® Usage Guidance and restrictions Maximum altitude 2,000m. Temperature range between 4°C and 65°C. Maximum relative humidity 80% for temperatures up to 31 decreasing linearly to 50% relative humidity at 40 ...
2. Remove the tray. 3. Peel the back off the loading guide and carefully apply the loading guide directly to the gel platform. The unit is now ready to be used. Gel Preparation The Table below shows the volume of agarose solution required to make the desired agarose gel for each unit tray size.
Gel Pouring The multiSUB range of units allows two different methods of gel casting: ® 1. Flexicaster 2. Traditional Tape Flexicaster 1. Level the Flexicaster base by adjusting the feet so that the bubble is exactly central. 2. Insert the desired length tray into the Flexicaster such that one end of the tray is pushed up and seals against the silicone mat of the permanent end of the Flexicaster.
Tape 1. Autoclave or plastic backed general tape should be used. A length 5cm longer than the width of each end of the tray should be cut. One length should be placed over one end of the tray and stuck m1cm in from the tray edge.
Gel Staining and Viewing The Multi Sub trays allow staining to be performed without removing the gel from the tray if this is preferred. 1. Transfer the gel to a vessel containing the appropriate volume of 0.5 µg/ml ethidium bromide stain for 15–30 minutes, see solutions for stock stain concentration and adjust to the volume used accordingly.
10X TBE stock (1L) dissolve in 750 ml distilled water: 108 g tris base (FW = 121) 55 g boric acid (FW = 61.8) 40 ml 0.5 M EDTA (pH 8.0) Fill to 1 litre final volume with distilled water Loading Dye 10x sample buffer stock consists of 50% glycerol, 0.25% bromophenol blue, and 0.25% xylene cyanole FF in 1x TAE buffer.
Decrease agarose percentage. Bands sharp but not enough high Review enzyme activity, digest bands seen Incomplete digestion further. Consult Cleaver Scientific about agarose. Agarose has improper endosmosis Reduce salt concentration to ≤0.1M. Salt concentration in sample too Reduce voltage. See high electrophoresis instructions.
Care and Maintenance Cleaning Horizontal Units Units are best cleaned using warm water and a mild detergent. Water at temperatures above 60°C can cause damage to the unit and components. The tank should be thoroughly rinsed with warm water or distilled water to prevent build-up of salts, but care should be taken not to damage the enclosed electrode and vigorous cleaning is not necessary or advised.
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Warranty The Cleaver Scientific Ltd. (CSL) multiSUB Horizontal Electrophoresis units ® have a warranty against manufacturing and material faults of twelve months from date of customer receipt. If any defects occur during this warranty period, CSL will repair or replace the defective parts free of charge.
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