ChemoMetec NucleoCounter NC-250 Quick Manual
Viability and cell count, cell health and cell cycle
Hide thumbs
Also See for NucleoCounter NC-250:
- User manual (65 pages) ,
- Instrument user manual (36 pages) ,
- Quick manuals (9 pages)
Subscribe to Our Youtube Channel
Related Manuals for ChemoMetec NucleoCounter NC-250
Summary of Contents for ChemoMetec NucleoCounter NC-250
- Page 1 Quick Guide Viability and Cell Count, Cell Health and Cell Cycle in one instrument! NucleoCounter ® NC-250 ™...
- Page 2 How to get started Dear NucleoCounter Customer, Thank you for purchasing the NucleoCounter NC-250 which offers not only fast and precise ® ™ Viability and Cell Count Assays, but also fast and easy assaying of cell cycle profiles with top class precision and the fastest apoptosis assay! The NucleoCounter NC-250...
- Page 3 How to Perform Viability and Cell Count Analysis Fast and Precise Cell Count and Viability Assays! The NucleoCounter NC-250 from ChemoMetec is a step forward for automated ® ™ cell counting. Select the desired protocol from the menu: for example, ‘Viability and Cell Count Assay’...
- Page 4 Optional: Visual Inspection of Counting Gates Click the green dot in the right-hand corner of the image window. OPTION This activates the image overlay function indicating all the events in the total cell count. The mouse scroll button allows the user to zoom in at the cursor position.
- Page 5 How to Perform a 2-Step Cell Cycle Analysis The NucleoCounter NC-250 provides fast and easy assaying of cell cycle profiles with top ® ™ class precision. Add 20 μl Solution 12 to 980 μl Solution 10 and mix Wash 5x10 to 1x10 cells for cells in suspension or adherent cells in a 6-well plate with PBS...
- Page 6 How to Perform a Cell Health Analysis The NucleoCounter NC-250 provides cell health measurements by the world’s fastest ® ™ apoptosis assay. VitaBright-48 a component of Solution 6 instantly fluoresces when it ™ binds thiols inside cells. The level of thiols is a direct measurement of cell vitality and a drop in the thiol level is concomitant with apoptosis.
- Page 7 Only if Required: Create your own version of a Protocol with Adjusted Generic Gates Perform the desired type of assay on a sample of the cells to be analysed. In the Tools menu select Protocol Adaptation Wizard. Follow the instructions in the Protocol Adaptation Wizard to create your own adapted protocol.
- Page 8 Copyright Notices Copyright © ChemoMetec A/S 2012. All rights reserved. No part of this publication and referred documents may be repro- duced, stored in a retrieval system or transmitted in any form or by any means, electronic, mechanical, photocopying, record- ing or otherwise, without the prior written consent of ChemoMetec A/S, Gydevang 43, DK-3450 Allerod, Denmark.
Need help?
Do you have a question about the NucleoCounter NC-250 and is the answer not in the manual?
Questions and answers