ChemoMetec NucleoCounter NC-250 Instrument User Manual
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Summary of Contents for ChemoMetec NucleoCounter NC-250
- Page 1 ® ™ NucleoCounter NC-250 Instrument user guide P/N 991-0251 Revision 1.4 September 2020 ChemoMetec A/S Gydevang 43 · DK-3450 Allerod · Denmark Telephone: (+45) 48 13 10 20 Website: www.chemometec.com E-mail: contact@chemometec.com...
- Page 2 The material in this document and referred documents is for information only and is subject to change without notice. While reasonable efforts have been made in preparation of these documents to assure their accuracy, ChemoMetec assumes no liability resulting from errors or omi ssions in these documents, or from the use of the information contained herein.
- Page 3 Date & Signature will appear on the instrument’s actual Declaration of Conformity statement, accompanying each new instrument order. Name and address of manufacturer: ChemoMetec A/S Gydevang 43 DK-3450 Allerod DENMARK www.chemometec.com 991-0251 Rev.
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Page 4: Weee Directive Information - Europe Only
WEEE directive information – Europe only WEEE directive information – Europe only Correct Disposal of This Product (Waste Electrical & Electronic Equipment) - Europe only This marking shown on the product or its literature, indicates that it should not be disposed together with other household wastes at the end of its working life. -
Page 5: Introduction And Intended Use
Introduction and intended use Introduction and intended use The NucleoCounter® NC-250™ is a compact cell-analyzer intended to be used for advanced cell analysis within the life-science and pharmaceutical industries. The NucleoCounter® NC-250™ system is intended for research use only, not for diagnostic use. The NucleoCounter®... -
Page 6: Warnings And Precautions
The NucleoCounter® NC-250™ shall only be used with an external power supply purchased from ChemoMetec. The detachable DC power supply cord set and appliance inlet of the external power supply are considered as the disconnecting device. - Page 7 Warnings and precautions protection provided by the NucleoCounter® NC-250™ and the external power supply may be impaired. Electromagnetic interference NOTE: This equipment has been tested and found to comply with the limits for a Class B digital device, pursuant to Part 15 of the FCC Rules. These limits are designed to provide reasonable protection against harmful interference in a residential installation.
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Page 8: Table Of Contents
Table of contents Table of contents DECLARATION OF CONFORMITY - EXAMPLE ..............III WEEE DIRECTIVE INFORMATION – EUROPE ONLY .............. IV INTRODUCTION AND INTENDED USE ................. V WARNINGS AND PRECAUTIONS ..................VI INSPECTION, UNPACKING AND INSTALLATION OF EQUIPMENT ........3 ®... - Page 9 Table of contents EQUIPMENT AND ACCESSORIES ................19 GENERAL DISCLAIMER ..................20 APPENDIX A: CHANGES TO USER GUIDE FROM LAST REVISION .......... 21 APPENDIX B: WEEE DIRECTIVE INFORMATION IN MORE EU LANGUAGES ......22 991-0251 Rev. 1.4 September-2020...
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Page 10: Inspection, Unpacking And Installation Of Equipment
1 Inspection, unpacking and installation of equipment 1 Inspection, unpacking and installation of equipment Upon receiving the shipment from ChemoMetec, the box or boxes should be carefully inspected for damage that may have occurred during shipping. Any damage must be reported to the carrier and to ChemoMetec immediately. -
Page 11: Cell Analysis With Nucleocounter® Nc-250
2 Cell analysis with NucleoCounter® NC-250™ 2 Cell analysis with NucleoCounter® NC-250™ The NucleoCounter® NC-250™ is a bi-color fluorescent cell-analyzing instrument. The user requires only a short period of training on how to operate the instrument before running highly advanced cell analysis protocols. The instrument itself is calibration -free. Individual calibration parameters for each slide will be recorded and used by the instrument during analysis. -
Page 12: Fluorescence Microscope
2 Cell analysis with NucleoCounter® NC-250™ 2.1.1 Fluorescence microscope The NucleoCounter® NC-250™ is a fluorescent microscope with a magnification of approximately 1.75×. It is equipped with three light sources and a dual-band emission filter. Types of sample slides that fit into the sample tray: •... - Page 13 2 Cell analysis with NucleoCounter® NC-250™ Figure 2. The NC-Slide A8™ (8-chamber slide) and NC-Slide A2™ (2-chamber slide). Note that arrows show the direction of loading the slide into the instrument 991-0251 Rev. 1.4 September-2020...
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Page 14: Buttons, Led Indicator And Pc Control
3 Buttons, LED indicator and PC control 3 Buttons, LED indicator and PC control Interactive controls RUN and EJECT buttons Tri-color status indicator Lid covering sample tray Figure 3. The front of the NucleoCounter® NC-250™ The figure above shows the front of the NucleoCounter® NC-250™. Three operational areas are present: •... -
Page 15: Start-Up
4 Start-up 4 Start-up Power on, power off At the rear of the instrument, there are two plug inlets and one fuse holder. When seen from the rear, the USB 2.0 port is to the left and the fuse holder and DC power inlet to the right. -
Page 16: Starting The Instrument
4 Start-up 4.1.1 Starting the instrument Once the NucleoView™ NC-250™ software is launched, the actuators of the instrument perform a homing procedure to assure correct positioning. After initialization, the status indicator on the front will turn green and the NucleoCounter® NC-250™ is ready for operation. -
Page 17: Operation Of Nucleocounter® Nc-250
5 Operation of NucleoCounter® NC-250™ 5 Operation of NucleoCounter® NC-250™ Ready mode Once the NucleoCounter® NC-250™ and the NucleoView™ NC-250™ software are ready, analysis can begin. In the NucleoView™ interface, use the browse menu to select the protocol to be performed. Once selected, either press the RUN button on the front of the instrument (Figure 1) or click on the Run icon in the software to execute the analysis . -
Page 18: Presentation Of Results
5 Operation of NucleoCounter® NC-250™ Presentation of results Depending on the type of analysis performed, result will be presented on the PC within a few seconds and the fluorescent image(s) will be stored on the hard disk. The typical process time is between 20 seconds to 2 minutes for multi-image and bi-color analysis. Protection of the optical system The lid covers the sample tray. -
Page 19: Nucleoview™ Nc-250
6 NucleoView™ NC-250™ 6 NucleoView™ NC-250™ Figure 8. NucleoView™ NC-250™ NucleoView™ NC-250™ software controls the NucleoCounter® NC-250™ instrument and analyzes the images recorded. NucleoView™ NC-250™ is located on a USB flash drive supplied with the instrument and contains the software, drivers, instrument specifications, the NucleoView™ NC-250™ user guide, etc. -
Page 20: Maintenance Of Nucleocounter® Nc-250
7 Maintenance of NucleoCounter® NC-250™ 7 Maintenance of NucleoCounter® NC-250™ Cleaning 7.1.1 Instrument enclosure Depending on the environment in which the NucleoCounter® NC-250™ is operated, we recommend carrying out regular cleaning of the outer surfaces. When cleaning the enclosure, use a soft moist cloth and gently wipe the surface. Any contamination, which does not come off immediately, should be rubbed gently with a cloth wetted with mild detergent. -
Page 21: Liquid Spill
If the contamination is present on every ima ge taken no matter which view is used it is most likely that other optical parts are contaminated. In this case contact ChemoMetec support. Figure 10 shows an example of a contamination in the top right quarter. -
Page 22: Troubleshooting: Error Messages
Under certain conditions, the NucleoView™ NC-250™ interface will display instrument error messages during operation. Corrective actions are suggested below , but if they do not correct the errors, contact ChemoMetec or your local distributor. Under certain conditions, the NucleoView™ NC-250™ interface will display software error messages during operation. - Page 23 3. Inspect the fuse. If it needs replacement use one as described in Chapter 9 If the NucleoCounter® NC-250™ does not turn on after replacement of the fuse, or if the fuse was not broken, please contact ChemoMetec or your local distributor for further instructions.
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Page 24: Technical Specifications
9 Technical specifications 9 Technical specifications The NucleoCounter® NC-250™ Optics Lens with 1.75× magnification, 1/2.5” CMOS Sample device NC-Slide A2™ (catalog no. 942-0001) and NC-Slide A8™ (catalog no. 942-0003) disposable chamber slides (2 and 8 chambers per slide, respectively) Excitation (nm) 2 LED light sources with peaks at 365 and 500 nm and dark-field white light Emission (nm) -
Page 25: Sample Media
9 Technical specifications Sample media Storage and stability Refer to package insert for the appropriate sample media type. EU standards The NucleoCounter® NC-250™ complies with EU standards as follows: Directives Electromagnetic Compatibility (2004/108/EC). EMC Low Voltage Directive (2006/95/EC) LVD Machinery Directive (2006/42/EC) MD Standards EN 61326-1:2006 Electrical equipment for laboratory use EMC:... -
Page 26: Equipment And Accessories
10 Equipment and accessories 10 Equipment and accessories On www.chemometec.com an updated list of Equipment and Accessories for the NucleoCounter® NC-250™ are available. 991-0251 Rev. 1.4 September-2020... -
Page 27: General Disclaimer
, NC-Slide A2™, or NC-Slide A8™ shall be construed to imply any warranty or guarantee. ChemoMetec and affiliated companies shall not be held liable for damages and customers shall indemnify ChemoMetec and affiliated companies against liability flowing from use of potentially inaccurate data generated by the ... -
Page 28: Appendix A: Changes To User Guide From Last Revision
Appendix A: Changes to user guide from last revision Appendix A: Changes to user guide from last revision 1. Changed from Revision 1.3 June 2016 to Revision 1.4 September 2020. 2. Since Microsoft has announced the discontinuation of the support for Windows 7 and 8, NucleoView™... -
Page 29: Appendix B: Weee Directive Information In More Eu Languages
Appendix B: WEEE directive information in more EU languages Appendix B: WEEE directive information in more EU languages United Correct Disposa l of This Product Kingdom (Waste Electr ical & Elec tronic Equ ipment) - Europe only This marking show n on the product or its literature, indicates that it should not be disposed t ogethe r with othe r household wastes at the e nd of its working life. - Page 30 Appendix B: WEEE directive information in more EU languages Norway Korrekt avhending av dette produkt (Avfall elektrisk og elektron isk utstyr) - Kun Europa Denne merkingen som vises på produktet eller dens dokument asjon, i ndikere r at de n ikke skal kastes sammen med annet hus holdni ngsavfall ved slutten av sin levetid.
- Page 31 Appendix B: WEEE directive information in more EU languages Finland Tämän tuotteen turvallinen hävittämin en (elektroniikka ja sähkölaitteet) - Vain Eu rooppa Oheinen me rkintä tuotteessa tai tuotteen oheismate riaalissa merkitsee, et tä tätä t uotetta ei tule hävittää kotitalousjättee n mukana sen elinkaaren päätyttyä.
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