ChemoMetec NucleoCounter NC-200 Quick Manual
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Summary of Contents for ChemoMetec NucleoCounter NC-200
- Page 1 Quick Guide to the easiest cell count ever using Reagent A100 + Reagent B NC-200 ™ ® NucleoCounter...
- Page 2 How to get started ® Dear NucleoCounter Customer, Thank you for purchasing the NucleoCounter® NC-200™ which offers the easiest one-step viability and cell count ever. ® The NucleoCounter NC-200™ Concept One step viability and cell count No pre-treatment ...
- Page 3 Our FAS are certified and expertly trained to identify, isolate, and resolve even the most challenging issues. You have direct access to our FAS during office hours. After hours, you can reach out via service@chemometec.com to resolve any issues and your local FAS will respond in a timely manner.
- Page 4 How to Perform Total Cell Count and Viability of Heavily Aggregated Cells Select the protocol from the menu ‘Viability and Cell Count - A100 Assay’. Optional: Enter Sample ID and Operator Name. Retain an aliquot of at least 200 µl of the original cell suspension for analysis in step 5. Add equal volumes of Reagent A100 and your cell sample to a microcentrifuge tube and mix.
- Page 5 PDF reports Right-click on file to create a PDF report. Select the parameters that will be visible on the report, and the properties of the parameters. Optional: Preview your result. Save and/or print your report to the default printer. Tip: Batch exports can be done from the NucleoView™ File Browser.
- Page 6 Optional: Visual Inspection of Counting Gates Option One (Preferred) Click the green dot in the right-hand corner of the image window. This activates the image overlay function indicating all the events in the total cell count. The mouse scroll button allows the user to zoom in at the cursor position. Cells will be framed by a pink square. Option Two (Advanced) Right-click on the sample file name in the ‘Data folders and files’...
- Page 7 Only if required: Create your own protocol with adjusted counting gates Perform the desired type of assay on a sample of the cells to be analyzed. In the Tools menu select Protocol Adaptation Wizard or right-click on the desired file and select ‘Start Protocol Adaption Wizard’.
- Page 8 The material in this document and referred documents is for information only and is subject to change without notice. While reasonable efforts have been made in preparation of these documents to assure their accuracy, ChemoMetec A/S assumes no liability resulting from errors or omissions in these documents, or from the use of the information contained herein.
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