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ChemoMetec NucleoCounter NC-250 User Manual

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NucleoCounter
NC-250
Instrument User's Guide
P/N 991-0251
Revision 1.3

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Summary of Contents for ChemoMetec NucleoCounter NC-250

  • Page 1 ® ™ NucleoCounter NC-250 Instrument User's Guide P/N 991-0251 Revision 1.3...
  • Page 2 This page was intentionally left blank 991-0251 Rev. 1.3 June-2016...
  • Page 3 P / N 9 9 1 - 0 2 5 1 ( E n g l i s h ) R e v i s i o n 1 . 3 J u n e , 2 0 1 6 ChemoMetec A/S Gydevang 43, DK-3450 Allerod, Denmark Telephone: (+45) 48 13 10 20, Fax: (+45) 48 13 10 21 Internet: www.chemometec.com,...
  • Page 4 This page was intentionally left blank 991-0251 Rev. 1.3 June-2016...
  • Page 5 The material in this document and referred documents is for information only and is subject to change without notice. While reasonable efforts have been made in preparation of these documents to assure their accuracy, ChemoMetec A/S assumes no liability resulting from error s or omissions in these documents, or from the use of the information contained herein.
  • Page 6 This page was intentionally left blank 991-0251 Rev. 1.3 June-2016...
  • Page 7 (1) this device may not cause harmful interference, and (2) this device must accept any interference received, including interference that may cause undesired operation. Date: 2012-Sept-3 Signed: Name: Sarah R. Rasmussen Position: QA/QC Managers Name and address of manufacturer: ChemoMetec A/S Gydevang 43 DK-3450 Allerod DENMARK www.chemometec.com 991-0251 Rev. 1.3 June-2016...
  • Page 8 Declaration of Conformity This page was intentionally left blank 991-0251 Rev. 1.3 June-2016 viii...

  • Page 9: Weee Directive Information - Europe Only

    WEEE directive information – Europe only WEEE directive information – Europe only Correct Disposal of This Product (Waste Electrical & Electronic Equipment) - Europe only This marking shown on the product or its literature, indicates that it should not be disposed together with other household wastes at the end of its working life.
  • Page 10 WEEE directive information – Europe only This page was intentionally left blank 991-0251 Rev. 1.3 June-2016...

  • Page 11: Introduction And Intended Use

    Since no tubes, valves, hoses etc. are present the instrument is virtually service and maintenance free. The NucleoCounter® NC-250™ part number at ChemoMetec A/S is 900-0251. 991-0251 Rev. 1.3 June-2016...
  • Page 12 Introduction and intended use This page was intentionally left blank 991-0251 Rev. 1.3 June-2016...

  • Page 13: Warnings And Precautions

    The NucleoCounter® NC-250™ shall only be used with an external power supply purchased at ChemoMetec A/S. The detachable DC power supply cord set and appliance inlet of the external power supply are considered as the disconnecting device.
  • Page 14 Warnings and precautions The user should be made aware of that, if the NucleoCounter® NC-250™ and the external power supply is used in a manner not specified by the manufacturer, the protection provided by the NucleoCounter® NC-250™ and the external power supply may be impaired.
  • Page 15 Warnings and precautions Never pipette by mouth. Avoid cross contamination of the samples when preparing the samples. This can compromise the quality of the results. 991-0251 Rev. 1.3 June-2016...
  • Page 16 Warnings and precautions This page was intentionally left blank 991-0251 Rev. 1.3 June-2016...

  • Page 17: Table Of Contents

    Table of contents Table of contents DECLARATION OF CONFORMITY ..................VII WEEE DIRECTIVE INFORMATION – EUROPE ONLY .............. IX INTRODUCTION AND INTENDED USE ................XI WARNINGS AND PRECAUTIONS ..................XIII INSPECTION, UNPACKING AND INSTALLATION OF EQUIPMENT ........3 CELL ANALYSIS WITH THE NUCLEOCOUNTER® NC-250™ ..........5 ®...
  • Page 18 1 Inspection, Unpacking and Installation of Equipment This page was intentionally left blank 991-0251 Rev. 1.3 June-2016...

  • Page 19: Inspection, Unpacking And Installation Of Equipment

    1 Inspection, Unpacking and Installation of Equipment 1 Inspection, Unpacking and Installation of Equipment Upon receiving the shipment from ChemoMetec A/S, the box or boxes should be carefully inspected for any damage that may have occurred during shipping. Any damage must be reported to the carrier and to ChemoMetec A/S immediately.
  • Page 20 1 Inspection, Unpacking and Installation of Equipment This page was intentionally left blank 991-0251 Rev. 1.3 June-2016...

  • Page 21: Cell Analysis With The Nucleocounter® Nc-250

    2 Cell analysis with the NucleoCounter® 2 Cell analysis with the NucleoCounter® NC-250™ The NucleoCounter® NC-250™ is a bi-color fluorescent cell-analyzing instrument. The NucleoCounter® has the advantage of short training time before the user can run highly advanced cell analysis protocols. The instrument itself is calibration free. The individual calibration parameter for a sample media will be read/used by the instrument during analysis.

  • Page 22: The Nucleocounter® Nc-250™ Instrument

    2 Cell analysis with the NucleoCounter® The NucleoCounter® NC-250™ instrument The NucleoCounter® NC-250™ is shown in the figure below. Run (top) and Eject keys PC with NucleoView NC-250™ analysis software Sample Tray Slides and reagents Figure 1. NucleoCounter® NC-250™ 2.1.1 Fluorescence microscope The NucleoCounter®...

  • Page 23: The Sample Tray

    2 Cell analysis with the NucleoCounter® 2.1.2 The sample tray. The sample tray holds the Slide during analysis in the instrument. There is one optical window in the tray. Please ensure this window is kept clean at all times. Refer to section “7.1 Cleaning”...
  • Page 24 2 Cell analysis with the NucleoCounter® This page was intentionally left blank 991-0251 Rev. 1.3 June-2016...

  • Page 25: Keys And Pc Control

    3 Keys and PC control 3 Keys and PC control Interactive Controls Run and Eject Keys Tri color status indicator Lid in front of the sample tray Figure 3. The front of the NucleoCounter® NC-250™ The figure above shows the front of the NucleoCounter® NC-250™. Three operational areas are present: ...
  • Page 26 3 Keys and PC control The third area has a status indicator.  Green - the NucleoCounter® NC-250™ is ready for next operation.  Red - the NucleoCounter® NC-250™ is busy.  Orange - the NucleoCounter® NC-250™ is not in connection with the NucleoView 250™...

  • Page 27: Start-Up

    4 Start-up 4 Start-up Power on, power off On the rear of the instrument two plugs and one fuse holder are located. When seen from the rear the USB 2.0 port is to the left and the fuse holder and DC Power Plug is at the right.

  • Page 28: Starting Up

    4 Start-up 4.1.1 Starting up Once the NucleoView NC-250™ software is installed and started, the actuators of the instrument perform a homing procedure in order to assure correct positioning of the different parts. After this initialization the status indicator on the front will turn green and the NucleoCounter®...

  • Page 29: Operation Of The Nucleocounter

    5 Operation of the 5 Operation of the NucleoCounter® Ready mode Once the NucleoCounter® NC-250™ and the NucleoView NC-250™ software are ready, analysis can begin. On the software’s main window the analysis (protocol) to be performed can be selected. Once selected, the keys on the front of the instrument (see Figure 1) or the buttons on the software’s main window can be used to eject or insert the sample tray and execute the analysis.

  • Page 30: Result Presentation

    5 Operation of the Result presentation Depending on the type of analysis performed the result will be present ed on the PC within a few seconds and the fluorescent image(s) will be stored on the hard disk. Typical process time is from 20 seconds to 2 minutes for multi image and bi-color analysis. Protection of the optical system The lid covers the sample tray.

  • Page 31: Nucleoview Nc-250™ Software

    5 Operation of the 6 NucleoView NC-250™ Software Figure 8. NucleoView NC-250™ software The NucleoView NC-250™ software is used for control of the NucleoCounter® NC-250™ instrument and image analysis of the recorded images. A USB Flash drive supplied with the instrument contains the software, drivers, instrument specific data etc.
  • Page 32 5 Operation of the Figure 10. Linkage between plot and image Refer to the NucleoView NC-250™ Software User’s Guide for additional information. 991-0251 Rev. 1.3 June-2016...

  • Page 33: Maintenance Of Nucleocounter® Nc-250

    In case cleaning of the window is needed, then refer to the Cleaning kit NucleoCounter® and follow the instructions here (ChemoMetec Part no: 911-0013). Figure 11 Illustration of how to clean the optical window with a clean and dry Alpha Swab.

  • Page 34: Spill Of Liquid

    If the contamination is present on every image taken no matter which view is used it is most likely that other optical parts are contaminated. In this case contact ChemoMetec A/S. An example of a relatively large contamination is given in Figure 12.

  • Page 35: Troubleshooting - Error Messages

    The CM files stored on the hard disk can vary from 1 5MB up to in some cases more than 50MB. Therefore it is important from time to time to check whether enough free space is present on the hard disk. ChemoMetec recommends a minimum of 10GBs free space on the hard disk.
  • Page 36 ChemoMetec A/S. If the NucleoCounter® NC-250™ does not turn on after replacement of the fuse, or if the fuse was not broken, please contact ChemoMetec A/S or the local distributor for further instructions. 991-0251 Rev. 1.3...

  • Page 37: Technical Specifications

    9 Technical specifications 9 Technical specifications The NucleoCounter® NC-250™ Optics Lens with x1.75 magnification, 1/2.5” CMOS Sample device NC-250™ disposable chamber slides (2 and 8 chambers per slide) Excitation (nm) 2 LED light sources with peaks at 365, 500 and dark field white light Emission (nm) Dual-Band emission filter: 410-460, 540-650 Sample consumption...

  • Page 38: Sample Medias

    9 Technical specifications Sample medias Storage and stability Refer to package insert for the appropriate sample media type. EU standards The NucleoCounter® NC-250™ complies with EU standards as follows. Directives Electromagnetic Compatibility (2004/108/EC). EMC Low Voltage Directive (2006/95/EC) LVD Machinery Directive (2006/42/EC) MD Standards EN 61326-1:2006 Electrical equipment for laboratory use EMC:...

  • Page 39: Equipment And Accessories

    10 Equipment and Accessories 10 Equipment and Accessories On www.chemometec.com an updated list of Equipment and Accessories for the NucleoCounter® NC-250™ are available. 991-0251 Rev. 1.3 June-2016...
  • Page 40 10 Equipment and Accessories This page was intentionally left blank 991-0251 Rev. 1.3 June-2016...

  • Page 41: Appendix A: Description Of Changes From Latest Revision

    Appendix A: Description of changes from latest revision Appendix A: Description of changes from latest revision This chapter describes changes from latest revi sion of this User’s guide. New:  Changes:  Front Page Revision 1.3  Page iii Revision 1.3 June 2016 ...

  • Page 42: Appendix B: Weee Directive Information In More Eu Languages

    Appendix B: WEEE directive information in more EU languages Appendix B: WEEE directive information in more EU languages United Correc t Disposa l of This Product Kingdom (Waste Electr ical & Elec tronic Equ ipment) - Europe only This marking show n on the product or its lite rature , indicates that it should not be disposed t ogethe r w ith othe r household w astes at the e nd of its work ing life.
  • Page 43 Appendix B: WEEE directive information in more EU languages Norway Korrekt avhending av dette prod ukt (Avfall elektrisk og elektron isk utstyr) - Kun Europa Denne merk ingen som vises på produktet elle r dens dokument asjon, indikere r at de n ikke skal kastes sammen med annet hus holdni ngsavfall ved slutten av sin levet id.
  • Page 44 Appendix B: WEEE directive information in more EU languages Finland Tämän tuotteen turvallinen hävittämin en (elektroniikka ja sähkölaitteet) - Vain Eu rooppa Oheinen me rkintä tuotteessa tai tuotteen ohe ismate ria alissa me rkitsee , että t ätä t uotetta e i tule hävittää kot italousjättee n mukana sen e linkaa ren päätyttyä.
  • Page 45 Appendix B: WEEE directive information in more EU languages This page was intentionally left blank 991-0251 Rev. 1.3 June-2016...
  • Page 46 NucleoCounter or NucleoCassette™ shall be construed to imply any warranty or guarantee. ChemoMetec A/S and affiliated companies shall not be held liable for damages and customers shall indemnify ChemoMetec A/S and affiliated companies against liability flowing from use of potentially inaccurate data generated by ...

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