ChemoMetec NucleoCounter NC-250 Quick Manuals
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- User manual (65 pages) ,
- Instrument user manual (36 pages) ,
- Quick manual (9 pages)
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Summary of Contents for ChemoMetec NucleoCounter NC-250
- Page 1 Quick Guide Viability and Cell Count, Vitality and Cell Cycle Assay in one instrument! NC-250 ™ ® NucleoCounter...
- Page 2 How to get started ® Dear NucleoCounter Customer, Thank you for purchasing the NucleoCounter® NC-250™ which offers not only fast and precise Viability and Cell Count Assays, but also fast and easy assaying of cell cycle profiles with top class precision and the fastest apoptosis assay! The NucleoCounter ®...
- Page 3 Cell Count Analysis Fast and Precise Cell Count and Viability Assays! The NucleoCounter® NC-250™ from ChemoMetec is a step forward for automated cell counting. Select the desired protocol from the menu: for example, ‘Viability and Cell Count Assay’ Choose the media type (NC-Slides A8™ or A2™)
- Page 4 Optional: Visual Inspection of Counting Gates OPTION 1 (PREFERRED) Click the green dot in the right-hand corner of the image window. This activates the image overlay function indicating all the events in the total cell count. The mouse scroll button allows the user to zoom in at the cursor position. Cells will be framed by a pink square. OPTION 2 (ADVANCED) Right-click on the sample file name in the ‘Data folders and files’...
- Page 5 How to Perform a 2-Step Cell Cycle Assay The NucleoCounter® NC-250™ provides fast and easy determination of cell cycle profiles with top class precision. Solution Solution Add 20 μl to 980 μl and mix Make a cell pellet of 5x10 to 1x10 cells Add 250 µl of the...
- Page 6 How to Perform a Vitality Assay The NucleoCounter® NC-250™ provides the world’s fastest Vitality assay. Vitabright48™ is a Solution component of instantly fluoresces when it binds thiols inside cells. The level of thiols is a direct measurement of cell vitality. Select the protocol from the menu: ‘Vitality Assay’...
- Page 7 PDF Reports Right-click on file to create a PDF report Select the parameters to be visible on the report, and the properties of the parameters Optional: Preview your result Save and/or print your report to the default printer Tip: Batch exports can be done from the NucleoView™ File Browser. Only if Required: Create your own version of a protecol with Adjusted Generic Gates...
- Page 8 The material in this document and referred documents is for information only and is subject to change without notice. While reasonable efforts have been made in preparation of these documents to assure their accuracy, ChemoMetec A/S assumes no liability resulting from errors or omissions in these documents, or from the use of the information contained herein.
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