ChemoMetec NucleoCounter NC-202 Instrument User Manual
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Summary of Contents for ChemoMetec NucleoCounter NC-202
- Page 1 ® ™ NucleoCounter NC-202 Instrument User Guide P/N 991-2020 Revision 1.1 November 2019 ChemoMetec A/S Gydevang 43 · DK-3450 Allerød · Denmark Telephone: (+45) 48 13 10 20 Website: www.chemometec.com E-mail: contact@chemometec.com...
- Page 2 The material in this document and referred documents is for information only and is subject to cha nge without notice. While reasonable efforts have been made in preparation of these documents to ensure their accuracy, ChemoMetec assumes no liability resulting from errors or omi ssions in these documents, or from the use of the information contained herein.
- Page 3 ; and ii) this device must accept any interference received, including interference that may cause undesired operation. Date: 2019-April-12th Signed: Name: Eva Louise Lange Position: Quality Manager Name and address of manufacturer: ChemoMetec A/S Gydevang 43 DK-3450 Allerod DENMARK www.chemometec.com 991-2020 Rev. 1.1 November 2019...
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Page 4: Weee Directive Information - Europe Only
WEEE Directive Information – Europe Only WEEE Directive Information – Europe Only Correct Disposal of This Product (Waste Electrical & Electronic Equipment) - Europe only This marking shown on the product or its literature, indicates that it should not be disposed of together with other household wastes at the end of its working life. -
Page 5: Introduction And Intended Use
The NucleoCounter® NC-202™ uses Via2-Cassettes™, which contain fluorescent dyes and allow the NucleoCounter® NC-202™ to detect cells. The NucleoCounter® NC-202™ is the third generation of NucleoCounter® cassette instruments, succeeding the NucleoCounter® NC-100 and NucleoCounter® NC-200™. The NucleoCounter® NC-202™ ChemoMetec part number is 900-2020. 991-2020 Rev. 1.1 November 2019... -
Page 6: Warnings And Precautions
The NucleoCounter® NC-202™ should only be used with an external power supply purchased from ChemoMetec. The detachable DC power supply cable set and the appliance inlet of the external power supply are considered as the disconnecting device. - Page 7 Warnings and Precautions Electromagnetic Interference NOTE: This equipment has been tested to meet the Class B digital device limits, pursuant to Part 15 of the FCC Rules. These limits are designed to provide reasonable protection against harmful interference in a residential installation. This equipment generates, uses and can radiate radio frequency energy and, if not installed and used in accordance with the instructions, may cause harmful interference to radio communications.
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Page 8: Table Of Contents
Table of Contents Table of Contents DECLARATION OF CONFORMITY ..................III WEEE DIRECTIVE INFORMATION – EUROPE ONLY .............. IV INTRODUCTION AND INTENDED USE ................. V WARNINGS AND PRECAUTIONS ..................VI INSPECTION, UNPACKING AND INSTALLATION OF EQUIPMENT ........3 ® NC-202™ ....3 NSTALLATION AND PERATION UALIFICATION OF... - Page 9 Table of Contents TECHNICAL SPECIFICATIONS ..................15 ® NC-202™ ................. 15 UCLEO OUNTER ....................15 AMPLE EDIA EU S ....................15 TANDARDS EQUIPMENT AND ACCESSORIES ................17 OTHER DOCUMENTATION ..................17 APPENDIX A: WEEE DIRECTIVE INFORMATION IN MORE EU LANGUAGES ......18 GENERAL DISCLAIMER ..................
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Page 10: Inspection, Unpacking And Installation Of Equipment
1 Inspection, Unpacking and Installation of Equipment Upon receiving the order from ChemoMetec, the box or boxes should be carefully inspected for any damage that may have occurred during shipping. Any damage must be reported to the carrier and to ChemoMetec immediately. -
Page 11: Cell Counting With Nucleocounter® Nc-202
Cell Counting with NucleoCounter® NC-202™ 2 Cell Counting with NucleoCounter® NC-202™ The NucleoCounter® NC-202™ is a dual-color fluorescent cell counting instrument. The NucleoCounter® NC-202™ is simple to operate, requiring little user input to execute e.g. cell counting protocols. The NucleoCounter® NC-202™ is operated by NC-View™ software. The NucleoCounter®... -
Page 12: The Run Button And Led Indicator
Cell Counting with NucleoCounter® NC-202™ New protocols are developed at an on-going basis and an up-to-date list of available protocols can be found at www.chemometec.com 2.1.3 The RUN button and LED indicator On the front of the NucleoCounter® NC-202™ there is a RUN button. Activating this will run the selected type of analysis on the instrument. -
Page 13: The Via2-Cassette
Cell Counting with NucleoCounter® NC-202™ The Via2-Cassette™ Figure 1: The Via2-Cassette™ contains two fluorescent dyes, AO (Acridine Orange) and DAPI (4',6-diamidino-2-phenylindole), deposited in the first part of the flow system 2.2.1 Loading the Via2-Cassette™ cassette The Via2-Cassette™ is loaded by pressing the white piston down while the cassette tip is immersed in the cell sample (Figure 3). -
Page 14: Via2-Cassette™ Volume Calibration Dot Code
Cell Counting with NucleoCounter® NC-202™ the instrument to i) move the piston to drive the cell sample through the mixing channels and into the measurement chamber; ii) fixate the cassette in the slot; and iii) record the fluorescence images, analyze data and present the counting result. Once analysis is complete, the sample and dyes are contained inside the cassette, which can be safely discarded. -
Page 15: Nucleocounter® Nc-202™ - Instrument Controls
3 NucleoCounter® NC-202™ – Instrument Controls 3 NucleoCounter® NC-202™ – Instrument Controls Interactive Controls Figure 3: Front of the NucleoCounter® NC-202™ The NucleoCounter® NC-202™ has two operational areas: • Cassette fixture covered by a yellow lid • RUN button and tri-color LED status indicator The lid is secured to the NucleoCounter®... -
Page 16: Nucleocounter® Nc-202™ Connections
3 NucleoCounter® NC-202™ – Instrument Controls NucleoCounter® NC-202™ Connections Rear view of the instrument (Figure 5) features the DC power inlet, fuse holder (Figure 6) and a USB 3.0 socket (Figure 7). The NucleoCounter® NC-202™ is delivered with a region-specific power cable to connect the external power supply to the main power supply. -
Page 17: Usb Connection And Initialization
USB connection and initialization The NucleoCounter® NC-202™ is connected using a USB 3.0 micro B – A or B – C cable. Only cables provided by ChemoMetec should be used with the instrument. Figure 6: USB 3.0 port Launching the NC-View™ software commences a short initialization sequence, which includes an instrument communication check and positioning of the piston motor. -
Page 18: Operation Of Nc-View™ Software And Nucleocounter® Nc-202™ Instrument
4 Operation of NC-View™ Software and NucleoCounter® NC-202™ Instrument 4 Operation of NC-View™ Software and NucleoCounter® NC-202™ Instrument NC-View™ Software The NC-View™ software is used for data acquisition, image analysis and display of results. For further details regarding NC-View™, refer to the NC-View™ Software User Guide (#991- 2022). -
Page 19: Piston Movements
4 Operation of NC-View™ Software and NucleoCounter® NC-202™ Instrument 4.2.1 Piston movements When a cassette is analyzed, the piston motor will move the piston rod down through the cassette cylinder. Hereby, the sample is transported to the cassette’s measurement chamber while sensors monitor the sample flow. When the sample has been loaded into the measurement chamber and reaches the end of the flow channel, the piston is stopped. -
Page 20: Maintenance Of Nucleocounter® Nc-202
Do not use compressed air from a single use-can, since these contain chemicals that can reduce optical performance. ChemoMetec’s support should be contacted if the contaminant cannot be removed with compressed air. -
Page 21: Troubleshooting For Nucleocounter® Nc-202
Figure 9 (turn counterclockwise). Inspect the fuse. If it needs replacement use one as described by ChemoMetec. If the NucleoCounter® NC-202™ does not turn on after replacing the fuse, or if the fuse was not blown, please contact ChemoMetec or your local distributor for further instructions. 991-2020 Rev. 1.1... -
Page 22: Technical Specifications
7 Technical Specifications 7 Technical Specifications NucleoCounter® NC-202™ Optics Lens with ×2.1 magnification, 2/3” CMOS Sample device Via2-Cassettes™ intended for NucleoCounter® NC-202™ Excitation (nm) 2 LED light sources with peak wavelengths at 365 nm and 505 nm Emission (nm) A single dual-band emission filter: 410-460 nm and 540-650 nm Sample consumption 70 μL Analyzed volume... - Page 23 7 Technical Specifications Standards EN 61326-1:2013 Electrical equipment for laboratory use EMC: EN 61000-3-2: 2014 Limits for harmonic current emissions EN 61000-3-3: 1995 +A1 +A2: 2013 Limitation of voltage changes, voltage fluctuations and flicker Standards EN 61010-1:2010 Safety requirements for electrical equipment for LVD: measurement, control, and laboratory use Standards...
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Page 24: Equipment And Accessories
An updated list of equipment and accessories for the NucleoCounter® NC-202™ are available from www.chemometec.com. 9 Other Documentation The table below shows a list of documents relevant for NucleoCounter ® NC-202™ operations. These documents can be downloaded from www.chemometec.com. Document Document Type Document number... -
Page 25: Appendix A: Weee Directive Information In More Eu Languages
Appendix A: WEEE Directive Information in More EU Languages Appendix A: WEEE Directive Information in More EU Languages United Correct Disposa l of This Product Kingdom (Waste Electr ical & Elec tronic Equ ipment) - Europe only This marking show n on the product or its literature, indicates that it should not be disposed of together with ot her house hol d wastes at t he e nd of its working life. - Page 26 Appendix A: WEEE Directive Information in More EU Languages Norway Korrekt avhending av dette produkt (Avfall elektrisk og elektron isk utstyr) - Kun Europa Denne merkingen som vis es på produktet eller dens dokument asjon, i ndikere r at de n ikke skal kastes sammen med annet hus holdni ngsavfall ved slutten av sin levetid.
- Page 27 Appendix A: WEEE Directive Information in More EU Languages Finland Tämän tuotteen turvallinen hävittämin en (elektroniikka ja sähkölaitteet) - Vain Eu rooppa Oheinen me rkintä tuotteessa tai tuotteen oheismate riaalissa merki tsee, että tätä t uotetta ei tule hävittää kotitalousjättee n mukana sen elinkaa ren päätyttyä.
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Page 28: General Disclaimer
NucleoCounter NC-202™ or Via2-Cassette™ shall be construed to imply any warranty or guarantee. ChemoMetec and affiliated companies shall not be held liable for damages and customers shall indemnify ChemoMetec and affiliated companies against liability resulting from use of potentially inaccurate data generated by the ...
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