ChemoMetec NucleoCounter NC-200 Quick Manual
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Summary of Contents for ChemoMetec NucleoCounter NC-200
- Page 1 Quick Guide to the easiest cell count ever! NucleoCounter ® NC-200 ™...
- Page 2 How to get started Dear NucleoCounter Customer, Thank you for purchasing the NucleoCounter NC-200 which offers the easiest one-step viability ® ™ and cell count ever. The NucleoCounter NC-200 Concept ® ™ One step viability and cell count No pre-treatment ...
- Page 3 How to Perform the One-step Viability and Cell Count Analysis Easiest cell count ever! The NucleoCounter NC-200 from ChemoMetec is a significant step forward for ® ™ automated cell counting. No need to add buffers or dyes. No need to calibrate.
- Page 4 How to Perform Total Cell Count and Viability of Aggregated Cells Select the protocol from the menu ‘Viability and Cell Count - Aggregated Cells Assay’ Optional: enter Sample ID and Operator Name Add equal volumes of ‘Solution 10’ and your cell sample to a microcentrifuge tube and mix.
- Page 5 PDF reports Right click on file to create a PDF report Select which parameters that will be visible on the report, and the properties of the parameters Optional: Preview your result Save and/or print you report to the default printer Tip: Batch exports can be done from the NucleoView™...
- Page 6 Optional: Visual Inspection of Counting Gates Click the green dot in the right-hand corner of the image window. OPTION This activates the image overlay function indicating all the events in the total cell count. The mouse scroll button allows the user to zoom in at the cursor position.
- Page 7 Only If Required: Create your own protocol with Adjusted Counting Gates Perform the desired type of assay on a sample of the cells to be analyzed. In the Tools menu select Protocol Adaptation Wizard or right-click on the de- sired file and select ‘Start Protocol Adaption Wizard’. Follow the instructions in the Protocol Adaptation Wizard to create your own adapted protocol.
- Page 8 Copyright Notices Copyright © ChemoMetec A/S 2012. All rights reserved. No part of this publication and referred documents may be repro- duced, stored in a retrieval system or transmitted in any form or by any means, electronic, mechanical, photocopying, record- ing or otherwise, without the prior written consent of ChemoMetec A/S, Gydevang 43, DK-3450 Allerod, Denmark.
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