Thermo Scientific 89852 User Manual

Pierce Peptide Desalting Spin Columns
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Catalog Numbers 89851 and 89852
Doc. Part No. 2162704 Pub. No. MAN0017280 Rev. A.0
Contents
Product
Pierce Peptide Desalting Spin Columns
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Product description
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The Thermo Scientific Pierce
The polymer-based hydrophobic resin contained in the column is stable from pH 1-14 and binds peptide samples with excellent recovery (typical
recovery is >90%) under reversed phase conditions. Columns can also be used for removal of excess hydrolyzed/quenched TMT reagents after
labeling to enable efficient and reliable downstream LC-MS analysis.
Procedure summary
Proteolytic digests of proteins extracted from cells or tissues are loaded onto an equilibrated desalting spin column. Peptides are bound to the
hydrophobic resin under acidic aqueous conditions and desalted by washing the column with acidified water by low-speed centrifugation. A
solution with acidified organic solvent (0.1% TFA, 50% acetonitrile) is then applied to the column to elute bound peptides by centrifugation.
Additional materials required
• Trifluoroacetic acid (TFA) (Product No. 28904)
• Acetonitrile (ACN), LC-MS Grade (Product No. 51101)
• Water, LC-MS Grade (Product No. 51140)
• Methanol (MeOH), Optima
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• Thermo Scientific Pierce
• Microcentrifuge with adjustable rotor speed up to 7,000 × g
• Vacuum centrifuge
Additional information
• Do not exceed the recommended centrifugation speeds as this may damage the column frit. Damaged frits may result in resin leakage, leading
to sample loss and/or damage to the LC system.
• Use low protein-binding microcentrifuge tubes to ensure maximum sample recovery.
• Avoid sample contamination and direct skin contact with solvents and chemicals. Always wear gloves when handling the spin columns and
samples.
For Research Use Only. Not for use in diagnostic procedures.
Cat. No.
89852
89851
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Peptide Desalting Spin Columns are optimized for desalting 5 µg to 5 mg of peptide sample from protein digests.
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LC-MS Grade (Fisher Scientific, Product No. A456-1)
Low Protein Binding Microcentrifuge Tubes, 2.0 mL (Product No. 88379 or 88380)
Contents
25 spin columns containing 20 mg of resin in a
1:1 water/DMSO slurry
50 spin columns containing 20 mg of resin in a
1:1 water/DMSO slurry
USER GUIDE
Storage
Store at 2-8°C.