Related Manuals for Rainin Pipetman P-2
Summary of Contents for Rainin Pipetman P-2
- Page 1 Pipetman ® continuously adjustable digital microliter pipettes 8 pipettes for volume ranges from 0.1 µL to 10 mL P-10 P-20 P-100 P-200 P-1000 P-5000 P-10ML Pipetman P-200 shown...
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Page 2: Table Of Contents
Pipetman, other Rainin pipettes, and disposable tips. Or use e-mail: tech.service@rainin.com Pipetman is a US-registered trademark of Rainin Instrument Company, Inc. Microman is a trademark of Gilson Inc. Pipetman pipettes are manu- factured under U.S. Patent Nos: 5,413,006 and 5,650,124. Other U.S. and international patents pending. -
Page 3: Description
Description Eight models of Pipetman continuously adjustable digital pipette cover the entire volume range from 0.1 µL to 10 mL. The P-200-M8 eight-channel Pipetman is also available, with a volume range of 20–200 µL, for conve- nience when using 96-well plates. Pipetman pipettes are not limited to fixed volume incre- ments —... -
Page 4: Autoclaving
A stainless steel tip ejector is provided with all models except P-5000 and P-10ML, for safe disposal of used tips. The ejector can be removed by pulling on the plastic collar on the ejector arm. Reattachment is quick and easy. Autoclaving The shaft and tip ejector are autoclavable: 121°C, 1 bar, 15–20 minutes. -
Page 5: Operation
Operation There are two ways of setting volume on Pipetman pipettes: with the volume adjustment knob, or by turning the plunger button. Both give exactly the same results. Volume Setting using Plunger Button 1a. Hold Pipetman in one hand. With thumb and forefin- ger, turn the plunger button counterclockwise until the volume indicator is ⁄... -
Page 6: Tip Selection
11. Allow the plunger to return to the up position. 12. Discard the tip by depressing the tip ejector button. A fresh tip should be used for each sample to prevent sample carryover. Figure 2 Operating Pipetman Tip Selection Tips must seal properly on the shaft to assure an air- tight seal and avoid leaks or poor accuracy. -
Page 7: Tip Immersion Depth
Prevent liquids from being drawn into the Pipetman shaft by taking the following precautions: 1. Use Rainin aerosol-resistant tips, with an internal filter which acts as a barrier to aerosols and liquids. 2. Never invert or lay Pipetman down if liquid is in the tip. -
Page 8: Pre-Rinsing Recommended
Pre-Rinsing Recommended Some solutions (e.g. serum, protein-containing solutions, and organic solvents) can leave a film on the inside tip wall, resulting in an error larger than the tolerance speci- fied. Since this film remains relatively constant in succes- sive pipettings with the same tip, excellent precision may be obtained by refilling the tip and using the refilled vol- ume as the sample. -
Page 9: Temperature Considerations
Temperature Considerations Warm or cold liquids can be measured with good preci- sion by using a consistent pipetting rhythm. This will help minimize any differences in heating or cooling effects within the pipette. Use a new tip each time for best accu- racy and precision when measuring samples with tem- peratures greatly different from ambient, and do not pre- rinse. -
Page 10: Troubleshooting And Repairs
4. If staining and/or corrosion of the piston is evident (due to previously dried sample material), do not use the pipette. Return to Rainin for service (see page 10). Never grease any Pipetman components, except for P-10ML pre- S/N N05803D, which use a grease seal. -
Page 11: Removing/Replacing Ejector Arm
Removing/Replacing Tip Ejector Arm Tip Ejector Arm Removal 1. Press tip ejector button. 2. Pull arm from handle using ejector arm release collar. Tip Ejector Arm Replacement 1. Press tip ejector button. 2. Hold Pipetman in one hand and the tip ejector arm in the other hand. -
Page 12: Service, Calibration And Repair
Service, Calibration and Repair Rainin maintains its own Pipette Repair and Calibration facilities in the following locations: Rainin Service Center Rainin Road, Woburn, MA 01801, USA Tel: 800-662-7027 Fax: 781-935-7631 Rainin Service Center 5400 Hollis St., Emeryville, CA 94608, USA... -
Page 13: Performance Specifications
(AB-15) for further information, or download a copy from Rainin’s website: http://www.rainin.com/papers.html When used in accordance with the pipetting procedures in this manual and with Rainin tips, Pipetman pipettes will perform to the following specifications. Accuracy Precision Model Volume... -
Page 14: Models P-2, P-10, P-20, P-100
P-10 P-20 P-100 Part numbers are listed on the back page. The plunger and plunger button in these diagrams are for dual-adjustment Pipetman, and cannot be used on older Pipetman models. For convenience, old-style part numbers are also listed on the back page. -
Page 15: Models P-200, P-1000, P-5000, P-10Ml
P-200 P-1000 P-5000 P-10ML... - Page 16 For P-10ML pre-S/N N05803D — same as listed above except: Plunger rod: 23861, Shaft: 6192-016, O-ring: 6192-015, Seal: n.a. ** Replacing the plunger rod will require recalibration of the instrument. Rainin is the only organization authorized to calibrate Pipetman pipettes. Please call 800-662-7027 for information.
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