HematologyAnalyzer Quick Reference Guide For Veterinary Use Only...
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Running a Blank Blanks must be run every 12 hours, after a Soak Cleaning has been performed, after a reagent pack change, and after the instrument has been restarted. It is not necessary to run blanks on days that samples are not run. Additionally, blanks need to be run after self tests and Deep Cleaning.
Hold the test tube at eye level and slowly invert the tube. If a clot is found, the sample should be discarded and a new sample should be drawn. Even small sized clots will affect the patient results and could damage your HM5. Running a Sample...
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Running a Sample 1. From the Home Screen, touch the Measure icon. 2. Touch Run. 3. Select the appropriate species Type. Optionally, input additional patient information. Touch Run. 4. Mix as directed and ensure the bubble moves from the top to the bottom of the sample tube to ensure all of the sample is mixing with the anticoagulant.
Controls should be run when reagent packs are changed, after a Soak Cleaning has been performed, and to check the calibration of the analyzer when results are in question. Remove the control from the refrigerator and allow to warm to room temperature for 15 minutes before use.
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If all values reported are within reference range, the analyzer is in calibration and samples may be run. If values are repeatedly out of range, consult Abaxis Technical Support before calibrating. (See Calibrating the Analyzer)
Use only the Abaxis Normal HM5 Control for calibration. Allow the control to come to room temperature by setting it on the counter for 15 minutes before use.
Changing the Reagent Pack Materials needed: HM5 Reagent Pack, HM5 Normal Control and latex or nitrile gloves. If “Change Reagent Pack” is used when installing new reagents, the analyzer will warn the user when there are fewer than 10 tests left in any given bottle. This feature will also track the number of samples, number of blanks, install date and the volume remaining in each of the bottles.
The wash head should be cleaned once a week. Warnings will alert users when a wash head cleaning is due. The warnings will differ depending on whether Scheduled Maintenance is Enabled or Disabled. If Scheduled Maintenance is Enabled, a message will appear when a cleaning is due. Touch Clean, then follow the on-screen instructions.
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Instructions will guide the user through the cleaning. This cleaning takes 10 minutes; after the cleaning is completed run a Blank, and if the Blank is acceptable, proceed with Quality control. If your HM5 analyzer is not set up for Soak Cleanings every 14 days, refer to HM5 Operator’s manual.
This cleaning is meant to be used as an emergency procedure when a high HCT trend is noticed: and it is not part of the regularly scheduled maintenance. Please ensure that your HM5 is on software version 2.3 or higher. 1. Deep Cleaning. Go to Maintenance> Cleaning> Deep Cleaning.
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Mix well by rolling the tube flat between your hands 10 times (A) and then slowly invert the tube 10-15 times (B). Run the well mixed HM5 control 2-3 times and ensure the results are within range to confirm the analyzer is ready to be used.
Visually inspect the wash head and chamber below it for blood debris; clean if necessary. Run a Soak Cleaning, then an automatic self-cleaning; repeat the Blank. More thorough troubleshooting procedures can be found in the Abaxis HM5 Operator‘s Manual. Warning Flags (Partial List)
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Warning Flags (Partial List) Results Warning Flags Flag Meaning Solution Empty sample Ensure the sampling depth is appropriate for the tube being used and that the tube is filled at least 1/2 full. If not, redraw a new sample. Lyse delivery issue Check Lyse (yellow) tube for kinks and loose connections at or very the bottle cap or dip tube and back of the analyzer and verify...
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Work List requires the use of additional VetScan connectivity products which enable bi-directional communication between the HM5 and compatible veterinary practice management software systems. 1. Order the CBC in the Practice Management Software. 2. From the Home Screen, touch the Measure icon then Work List.
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Using Work List 3. Select a patient from the list by checking the associated box. 4. Touch Run. 5. Follow the mixing directions on the screen. Touch Run. When the CBC is complete, the animal sample just run will no longer be displayed on the Work List.
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