Abaxis VetScan vs2 Operator's Manual
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Operator's Manual
For Veterinary Use Only
Customer and Technical Service:
1-800-822-2947
Available 24 hours a day, 7 days a week.
February 2009
PN: 1200-7008 Rev. D
© 2009, Abaxis, Inc.
Union City, CA 94587

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  • Page 1 ® Operator’s Manual For Veterinary Use Only Customer and Technical Service: 1-800-822-2947 Available 24 hours a day, 7 days a week. February 2009 PN: 1200-7008 Rev. D © 2009, Abaxis, Inc. Union City, CA 94587...
  • Page 2 Abaxis, VetScan, VetScan VS2, and iQC are registered trademarks of Abaxis. U.S. Patent Nos. 5,061,381, 5,275,016, 5,304,348, 5,403,415, 5,413,732, 5,457,053 © 2009, Abaxis, Inc. Union City, CA 94587...
  • Page 3: Table Of Contents

    Introducing the VetScan VS2 ........
  • Page 4 Test Procedure Summary ......... . 4-14 VetScan VS2 Chemistry Analyzer ....... . . 4-14 Reagent Rotors .
  • Page 5 Using Analyzer Messages ......... . Contacting Abaxis Technical Service ....... . .
  • Page 6 Section B: Supported Printers..........Printers Supported by the VetScan VS2 .......
  • Page 7: Section 1: Quick Reference Guide

    Quick Reference Guide Section 1 Analyzer Overview ® These diagrams show the VetScan VS2 Analyzer and its components. Front View Printer and cover Touchscreen Power button Rotor drawer Back View Printer and cover Handle USB ports DC power input Air filter CD drawer Quick Reference Guide...
  • Page 8: Installation Guidelines

    Installation Guidelines Step 1: Unpack the VetScan VS2 Analyzer and Accessories Immediately upon receipt, place the box of reagent rotors in a refrigerator (2–8 ºC or 36–46 ºF). Remove the analyzer from its box and place it on a sta- ble, level surface.
  • Page 9: Step 3: Install Printer Paper Roll

    Step 3: Install Printer Paper Roll Open the printer cover. If the printer contains a roll of printer paper, remove it from the printer. Remove the wrapper from the new paper roll, then unroll several inches of paper. Put the roll into the printer so that paper unrolls from the bottom of the roll and out towards the front of the analyzer, as shown at right.
  • Page 10: Step 4: Set System Parameters

    See Section 5‚ “Selecting Units” for instructions on chang- ing to SI units such as mmol/L. The VetScan VS2 is now ready to run samples. See Section 4‚ “Test Procedure and Results” for instructions. If You Have Problems If any of the following occurs, see Section 8‚...
  • Page 11: Guidelines For Obtaining And Using Samples

    Use only powder-free gloves, to keep powder off of analyzer optics. Use only reagent rotors that have not expired, as noted on the pouch and box. The VetScan VS2 Analyzer automatically rejects expired rotors. Do not remove the rotor from the refrigerator until you are ready to run the analy- sis.
  • Page 12 Guidelines for Obtaining Samples Serum samples: Use no-additive evacuated specimen collection tubes or serum separator tubes. Whole blood or plasma samples: Use only lithium heparin evacuated specimen collection tubes. Obtain a sample of approximately 100 µL. When collecting the sample in lithium heparin collection tubes, fill the tube at least half-way to maintain the proper ratio of sample to anticoagulant.
  • Page 13 Test Tube Order To prevent contamination, always fill test tubes in this order: Red top: No anticoagulant. Note: Use the red top test tube only if necessary. If the red top tube is not needed, fill the green top tube first. But when needed, the red top tube must be filled before any other.
  • Page 14: Step 3: Add Sample To The Rotor

    Syringe pre-wetted with lithium heparin. Make sure the heparin concentration is 15 units per mL of blood. Expel excess heparin by moving the plunger back and forth several times, until only a fine coating is left inside the syringe barrel. This is important for preventing excess sample dilution, which will alter final sample results and can cause errors.
  • Page 15 Using a Fixed 100 µL Volume Pipette Firmly attach a new tip to the end of the pipette. Do not touch the tip, or a false elevation of amylase can result. With your index finger or thumb, push the pipette plunger to the stop position, and hold it there for sample pickup.
  • Page 16: Step 4: Analyze The Sample

    Using a Lithium Heparinized Syringe The sample can be dispensed directly into the rotor from a heparinized syringe. Gently invert the syringe three or four times to mix well. Remove the needle from the syringe before dispensing the sample, to minimize hemolysis. See “Sample Delivery Volume”...
  • Page 17: Section 2: General Information

    CAUTION: If the VetScan VS2 Analyzer is used in any way other than described in this manual, the analyzer may not operate as intended, may produce inac- curate or no results, and may pose a safety haz- ard.
  • Page 18: Abaxis Technical Service

    Call Abaxis Technical Service, 24 hours a day, 7 days a week, at 1-800-822-2947. Symbols Used in Labeling The following symbols are found above the connectors or on the VetScan VS2 label, on the back of the analyzer. Symbol...
  • Page 19: Frequently Asked Questions

    Can the analyzer detect a flawed or defective rotor? Yes. The VS2’s extensive Intelligent Quality Control (iQC) detects any flaw or potential anomaly in the rotor. Abaxis also provides a Performance Guarantee covering manufac- ture-defective rotors. (This does not include cancellations or assay suppressions due to improper rotor storage, operator error, or inadequate sample integrity.)
  • Page 20: Analyzer Setup

    Does the VS2 need surge protection or an uninterruptable power supply? Abaxis recommends plugging the VS2 into a surge protector designed for use with a com- puter. Abaxis also recommends using an uninterruptable power supply (UPS) in areas prone to electrical surges or power outages.
  • Page 21: Samples

    Samples What types of samples can I run on the VS2? Whole blood, serum, or plasma. Is precision pipetting required? No. Adding approximately 100–120 l of sample is sufficient. (Make sure not to overfill the chamber, or internal spills can result.) The VS2’s on-board microfluidics handle the rest.
  • Page 22: Testing And Results

    Endogenous substances such as hemolysis, lipemia, and icterus affect all blood chemis- tries, regardless of the type, size, or cost of the analysis instrument. The VetScan VS2 is the only point-of-care analyzer that assesses and suppresses hemoly- sis, lipemia, and icterus by determining assay-by-assay the level at which these substances could affect the reliability of the results, and bringing this to the user’s attention.
  • Page 23 Can I back up the results on my VS2? No, but you can print out reference ranges and results, or transfer them for storage in your lab management software. Can patients other than equine, large animal, or avian use the Equine, Large Ani- mal, and Avian profiles? No.
  • Page 24 General Information...
  • Page 25: Section 3: Set Up And Analyzer Description

    VetScan VS2 operator’s manual, quick reference guide, warranty card, and start-up packet (not shown) Complete the warranty card and mail it to Abaxis within 10 days of installation to start the warranty period. Customers are placed on the customer mailing list to receive any information pertain- ing to the VS2 and ancillary products, such as software upgrades.
  • Page 26: Setting Up The Analyzer

    Setting Up the Analyzer Refer to the required environmental specifications in Section 3. Check that the VetScan VS2 is: On a sturdy, level surface, free of vibration and sudden jolts. In an ambient operating temperature of 15–32 ºC (59–90 ºF).
  • Page 27 Pull gently on the end of the paper to make sure it is taut. Press the Power button to turn on the analyzer. The following appears: VetScan VS2 V1.2.3.4 Performing iQC... Note: If no display message appears (blank display), remove the plug from the adapter, re-attach, and press in firmly.
  • Page 28 DOG, and HORSE animal types are pre-defined; these values can be changed using the Customizing Reference Range feature described in Section 5. Note: To order reagent rotors, pipettes, tips, and printer paper, contact an authorized distributor, or contact Abaxis Customer Service at 1-800-822-2947 during regular business hours. Set Up and Analyzer Description...
  • Page 29: Analyzer And Environmental Specifications

    Analyzer and Environmental Specifications Analyzer dimensions: Height: 32.4 cm (12.75 inches) Width: 15.2 cm (6 inches) Depth: 20.3 cm (8 inches) Weight: Analyzer: 5.1 kg (11.2 pounds) Power Adapter: 0.7 kg (1.6 pounds) Mode of operation: Continuous Protection against ingress of fluids: Ordinary equipment (IPXO) Altitude: 2000 m (6562 ft)
  • Page 30: Vetscan Vs2 Chemistry Analyzer Overview

    Note: Be sure to become familiar with the VS2 before running samples. The VetScan VS2 Chemistry Analyzer consists of a portable ana- lyzer and disposable single-use reagent rotors. Each reagent rotor contains all the reagents needed to perform a profile of tests on a single sample.
  • Page 31: Touchscreen

    Touchscreen The operator communicates with the analyzer through a touchscreen. Turn the analyzer on or off by pressing and holding the Power button on the front of the instrument. Note: Before powering off the analyzer, be sure to remove and dispose of any rotor in the instrument.
  • Page 32: Reagent Rotors

    Reagent Rotors The reagent rotors are specially designed to perform all the steps required to convert a few drops of blood into a panel of test results. The rotor spins, separating the whole blood sample into plasma and cells. Precisely measured quantities of plasma and diluent are mixed together and delivered to the reaction cuvettes along the rotor perimeter.
  • Page 33: Section 4: Test Procedure And Results

    Introduction to Testing Note: Use only VetScan Reagent Rotors with the VetScan VS2 Chemistry Analyzer. The basic testing procedure is listed below. The remainder of this section describes the details of collecting samples and preparing the reagent rotor (Section 4.2), sample processing (Section 4.4), and interpreting results (Section 4.6).
  • Page 34: Collecting Samples

    Press ANALYZE on the touchscreen to open the analyzer’s rotor drawer. Place the rotor in the drawer, then press CLOSE on the touchscreen. There is no need to orient the rotor in a particular direction. Analysis begins automatically when the drawer closes. Input patient information such as patient ID, operator ID, etc., as directed by the analyzer’s display.
  • Page 35: Adding A Sample To The Rotor

    Adding a Sample to the Rotor Remove the “practice” rotor from the start-up pack and practice loading a sample, using a preferred transfer device after reading the instructions in this section. CAUTION: The practice rotor is for sample loading practice only. DO NOT RUN THIS ROTOR IN THE VS2 —...
  • Page 36 Sample Delivery Volume Follow these guidelines to make sure you add the correct sample volume to the reagent rotor. When a sufficient quantity of sample is added, it will form a line between the arrows on the rotor surface. 90 µL If an air bubble is introduced, add enough sam- >...
  • Page 37: Running Tests

    Turn on the analyzer power by pressing the Power button on the front of the analyzer. Do this before removing reagent rotors from the refrigerator. The analyzer starts up, then VetScan VS2 V1.2.3.4 performs a self test. See Section 7.2, “Quality Control Features”...
  • Page 38 When the analyzer reaches operating temperature, it displays the main analysis screen. Note: Rotors can be used directly from the refrigerator. (Prolonged time at room temperature can cause sup- pression of chemistries and rotor cancellations.) Do not expose rotors — in or out of pouches — to tem- peratures higher than 32 ºC (90 ºF).
  • Page 39 A 100 L sample will fill the sample chamber and form a line between the two triangles printed on the rotor, as shown in the figure at right. The reagent rotor is designed to handle up to 120 L of 90 µL sample which will partially fill the well just beyond the two triangles.
  • Page 40 The following mes- Load Rotor sages are then dis- played: Close drawer to Opening Drawer... analyze a sample CLOSE Note: In the Load Rotor screen, press the information icon to view additional help screens outlining the basic analysis procedure. (Press Next and Back to move through the screens, and Exit to close them.) Insert Sample Insert Rotor...
  • Page 41 Enter the operator ID using the touchscreen, then press Enter Operator ID Done. 12345678901234 The operator ID is a number of up to 14 digits that is assigned by the facility. The right arrow key ( ) functions as a dash (–), and the left arrow key ( ) functions as a backspace.
  • Page 42 Enter the patient’s age using the up ( ) and down ( Enter Age arrow keys (be sure to adjust the units if needed), then press Done. Yrs. Back Done Enter an ID number for the patient (up to 14 characters), then press Done.
  • Page 43 The analyzer checks the rotor type, and begins processing the sample with no further input. When the sample is finished processing, the analyzer stores the results and shows that analysis is complete. By default, the analyzer automatically prints the results of the analysis. If the results do not print automatically, they can be recalled from memory and printed —...
  • Page 44: Canceling An Analysis

    CAUTION: Dispose of the rotor according to the lab’s standard procedures for animal samples. To analyze another sample, insert a new rotor and repeat the above procedure. When finished, press CLOSE to close the drawer and return the analyzer to standby mode. Canceling an Analysis To cancel an analysis in process, press CANCEL on the touch- Confirm...
  • Page 45: Using Test Results

    Using Test Results The results calculated by the analyzer are stored in memory and printed automatically, and can be recalled and printed later as needed. If the analyzer is connected to an external computer, the results are automatically transmitted as soon as they are calculated. See Section 9.2, “Principles of Operation”...
  • Page 46: Test Procedure Summary

    Section 8. Test Procedure Summary VetScan VS2 Chemistry Analyzer Check that the electrical outlet utilized for the analyzer is grounded. Check that the ambient temperature where the analyzer is located is 15–32 ºC (59–90 ºF).
  • Page 47: Reagent Rotors

    Reagent Rotors Do not use an expired rotor. The reagent rotor expiration date is printed on the foil pouch and is encoded in the bar code. Store all reagent rotors at 2–8 ºC (36–46 ºF) as described on their respective pouch labels.
  • Page 48: Handling Samples

    Handling Samples Use the following guidelines and procedures to ensure best results. Syringe Guide Small breed: up to 10 kg — use a 25-gauge syringe. Medium breed: 10–30 kg — use a 22-gauge syringe. Larger breed: 30–60 kg — use a 20-gauge syringe. Giant breed: over 60 kg —...
  • Page 49: Section 5: Configuring The Analyzer

    Configuring the Section 5 Analyzer This section describes how to configure the VetScan VS2 Chemistry Analyzer to achieve excellent performance. Make general analyzer settings as shown in “Using the Settings Screens” on page 5-2. Set species reference ranges for the specific patient population, and print, transmit, or archive these ranges.
  • Page 50: Using The Settings Screens

    Using the Settings Screens The following shows the general path through the menus for performing the procedures in this section. These procedures are available whenever the analyzer displays the Home screen. In the Home screen, press the Settings icon. The Analyzer Settings screen then opens. From here, adjust the date and time, language, sound, display, and printer, or view basic information about the analyzer.
  • Page 51: Modifying Reference Ranges Of A Single Analyte

    Select the reference range to modify. Include Reference Ranges To modify the last reference range that was changed, Last Modified: ALB press Last Modified, then skip to step 4 on page 5-4. To modify any reference range, press All, then continue to step 5.
  • Page 52: Modifying The Reference Ranges For A Species

    Select the reference range from the list. If needed, use the up ALB Ranges ) and down ( ) arrow keys to view the entire list. 3.3 - 5.5 g/dL g/dL 3.3 - 5.5 Equine 3.3 - 5.5 g/dL Cntl. 1 3.3 - 5.5 g/dL Cntl.
  • Page 53: Adding A Species

    The analyzer displays the ranges for the species. Species 1 Ranges 3.3 - 5.5 g/dL Select the analyte to adjust. If needed, use the up ( ) and 3 - 5 down ( ) arrow keys to scroll through the list. 3 - 6 2 - 4 1 - 3...
  • Page 54 Select Species 1 to create a reference range for a new, unnamed Add Species species, Control 1 to create a range for a new control level, or Species 1 Select to create a reference range for a predefined species. Control 1 Select Back Home...
  • Page 55 To create a new control: Press Control 1. Set minimum and maximum values for each of the Control 1 Limits analytes to be included in the range: 0.0 - 0.0 g/dL i. Select the analyte to adjust from the list. If 0 - 0 needed, use the up ( ) and down (...
  • Page 56 Select the species. Use the up ( ) and down ( Species Available arrow keys to scroll through the list. Amazon Budgerigar Caiques Canaries Cockatiel Back Home Set minimum and maximum values for each of the Amazon Ranges analytes to be included in the range. 0.0 - 0.0 g/dL i.
  • Page 57: Removing A Species

    Removing a Species Use this procedure to remove the reference ranges for a particular species. In the Modify Reference Ranges screen, press Species. Press Remove Species. Species Add Species Remove Species Factory Default Species Back Home Select the species to remove. Use the up ( ) and down ( Remove Species arrow keys to scroll through the list.
  • Page 58: Printing And Archiving Reference Ranges

    Printing and Archiving Reference Ranges Reference ranges can be printed, or stored in the analyzer as archive files for later use or review. In the Home screen, press the Settings icon. Press the More Settings icon. Press the Archive Reference Ranges icon. Press Send.
  • Page 59: Retrieving Reference Ranges

    To archive the ranges onto a PC connected to the analyzer: Press External Archive. Select the species reference range to store. Select Report The reference range is then stored on the PC under the current date and time. Horse Other Back Home To print a range:...
  • Page 60: Transmitting Reference Ranges

    A warning screen then appears. Press Continue to retrieve the WARRING archived reference range. Restoring Reference Ranges to 30 Nov 2005 12:01 PM Continue CANCEL Transmitting Reference Ranges Reference ranges can be transmitted to a connected PC and stored for later use or review. In the Home screen, press the Settings icon.
  • Page 61: Viewing Analyzer Identification

    Press External Archive. Send Reference Ranges Printer Internal Archive External Archive Back Home Select the species reference range to store. Select Report The reference range is then stored on the PC under the current date and time. Horse Other Back Home Viewing Analyzer Identification Use this function to verify information about the analyzer, such as serial number and the version...
  • Page 62: Changing Date And Time

    The display then shows the analyzer information. Changing Date and Time The date and time is factory preset to Pacific Time. Reset the date and time as needed when set- ting up the analyzer, and periodically thereafter. In the Home screen, press the Settings icon. Press the Date and Time icon.
  • Page 63: Selecting The Language

    Press Date when the time is set. Note: If the time entered is invalid, an error message appears — press Con- tinue, set the correct time, then press Done. Use the up and down arrow keys to adjust the day, month, and year.
  • Page 64: Selecting Units

    Select the keyboard type from the list. If needed, use the up and Select Keyboard Type down arrows to view the entire list. English (USA) Français Deutsch Italiano Español Back Home Selecting Units You can select either Common Units, such as mg/dL, or SI Units (Systeme International), such as mmol/L, to report results.
  • Page 65 Select the units to use for all analytes: All Units Non SI or SI. Non SI Back Home To set the units for a particular analyte: Press Single Analyte. Set Units All Analytes Analyte Groups Single Analyte Back Home Select the analyte from the list. If needed, use the Single Unit up ( ) and down (...
  • Page 66 Select the units to use for the analyte: g/dL, g/L, ALB Units mg/dL, mg/L, or umol/L. g/dL mg/dL mg/L umol/L Back Home To set units for all electrolytes: Press Analyte Groups. Set Units All Analytes Analyte Groups Single Analyte Back Home Press Electrolytes.
  • Page 67 Select the units to use for all electrolytes: Electrolyte Units mmol/L or mEq/L. mmol/L mEq/L Back Home To set units for all enzymes: Press Enzymes. Group Units Electrolytes Enzymes Lipids Minerals Proteins Back Home Select the units to use for all enzymes: Enzymes Units U/L or ukat/L.
  • Page 68 To set units for all lipids: Press Lipids Group Units Electrolytes Enzymes Lipids Minerals Proteins Back Home Select the units to use for all lipids: mg/dL or Lipids Units mmol/L mg/dL mmol/L Back Home To set units for all minerals: Press Minerals.
  • Page 69 Select the units to use for all minerals: Minerals Units mg/dL, mmol/L or mEq/L. mg/dL mmol/L mEq/L Back Home To set units for all proteins: Press Proteins. Group Units Electrolytes Enzymes Lipids Minerals Proteins Back Home Select the units to use for all proteins: Proteins Units g/dL or g/L.
  • Page 70: Setting Sound Volumes

    5.10 Setting Sound Volumes Use this procedure to adjust the volume of the sounds used for the analyzer’s screen click, alert, and status notification. In the Home screen, press the Settings icon. Press the Sound Settings icon. Select the sound to adjust. Sound Settings Screen Click Alerts...
  • Page 71: Adjusting The Display

    5.11 Adjusting the Display You can adjust the brightness of the analyzer’s display as needed, and calibrate the display’s touchscreen for best performance. In addition, you can set the period that the analyzer must be inactive before it activates a screen saver or enters power-saving mode. (For either, touch the screen to wake the analyzer.) In the Home screen, press the Settings icon.
  • Page 72 To adjust the screen saver delay: Press Screen Saver. Display Settings Back Light Screen Saver Power Save Calibrate Back Home Use the controls to set the delay. Screen Saver Wait Use the up ( ) and down ( ) arrow keys Current to adjust the delay.
  • Page 73 Use the controls to set the delay. Screen Saver Wait Use the up ( ) and down ( ) arrow keys Current to adjust the delay. Never 15 min Press Never to prevent the analyzer from Never entering power-saving mode. Default Press Default to set the delay to its factory default.
  • Page 74: Configuring Printers

    5.12 Configuring Printers Use these procedures to set up and configure the analyzer’s internal printer, or an external printer connected to the analyzer. In the Home screen, press the Settings icon. Press the Printer Settings icon. You can now do the following: Printer Setup To select the default printer to be used with the analyzer, Configure...
  • Page 75: Testing A Printer

    Printers.” Note: Printer capabilities are subject to change without notice. For an up-to- date list of printers supported by the VetScan VS2, contact Abaxis Tech- nical Service 24 hours a day, 7 days a week, at 1-800-822-2947. Configuring the Analyzer...
  • Page 76: Selecting Reports

    Selecting Reports Use this procedure to set the type and number of reports that can be printed automatically after a sample is analyzed. In the Printer Setup screen, press Configure. Press Select Reports. Configure Printer Set Default Select Reports Back Home Select the report type.
  • Page 77: Setting Communication Protocol

    Press the Communication Settings icon. Select a protocol: ASCII Text, None, ASTM, or XML. Note: To use ASTM, contact Abaxis Technical Service 24 hours a day, 7 days week, at 1-800-822-2947. If you use veterinary management software, please contact your software supplier to ensure that your software is compatible with the VetScan VS2.
  • Page 78: Setting Optional Advanced Functions

    5.14 Setting Optional Advanced Functions The VS2 includes a number of optional data-entry functions that can be used to enter more detailed information about samples, such as age and gender. The analyzer can be configured to use some or all of these functions as needed. Note: In this manual, screens for optional advanced functions are marked with this icon:...
  • Page 79 For Alternate ID, select the particular alternate ID item to be Alternate ID entered. Disabled Alternate ID Use the up ( ) and down ( ) arrow keys to scroll through the Doctor ID list. Owner ID Phone Number Back Disable Enable Configuring the Analyzer...
  • Page 80 5-32 Configuring the Analyzer...
  • Page 81: Section 6: Recalling Results

    Recalling Results Section 6 The VetScan VS2 Analyzer includes a Recall function that provides access to the results of the most recent tests, including patient results, control results, and test errors. The Recall function is available from the analyzer’s Home screen.
  • Page 82: Recalling The Last Rotor Results

    Recalling the Last Rotor Results Use this procedure to recall, print, or transmit the results of the last rotor run. In the Home screen, press the Recall icon. Press Last Rotor. The results from the last rotor are displayed for review. You can use the up ( ) and down ( ) arrow keys to scroll through the...
  • Page 83: Searching Results

    Searching Results Use the Recall function to search for a particular record according to its patient ID number or date, or to view a list of all patient or control results sorted by date. In the Home screen, press the Recall icon. Press Search.
  • Page 84: Searching For Results By Patient/Control Id

    Searching for Results by Patient/Control ID In the Search screen, press Patient/Control ID. Enter the patient ID number. Use the right arrow key ( ) to enter dashes (–), and the left arrow key ( ) to backspace. Press Search. The analyzer then displays a list of results (Patient, Control, and Error) sorted first by ID, then by date.
  • Page 85: Searching For Results By Date

    To print these results or transmit them to an external computer: Press Print. Select Print and/or Transmit. Select Report Select Results, iQC, Error Report, or All. Results The display shows “Sending Report...” while the report or results are printing or transmitting. Error Report Print Xmit...
  • Page 86: Viewing Patient Results Or Control Results By Date

    Press to display the results. Use the up ( ) and down ( ) arrow keys to scroll through the results. To print these results or transmit them to an external computer: Press Print. Select Print and/or Transmit. Select Report Select Results, iQC, Error Report, or All.
  • Page 87: Browsing Results

    Press to display the results. Use the up ( ) and down ( ) arrow keys to scroll through the results. To print these results or transmit them to an external computer: Press Print. Select Print and/or Transmit. Select Report Select Results, iQC, Error Report, or All.
  • Page 88 The analyzer then displays a list of all results (Patient, Control, All Results by Date and Error) in reverse chronological order, beginning with the 29 Aug 12345678901234 date entered. 29 Aug 12345678901234 Use the up ( ) and down ( ) arrow keys to scroll through the 28 Aug 12345678901234...
  • Page 89: Transmitting Results

    Transmitting Results Use this procedure to transmit all stored results to a computer. Note: The analyzer must be connected to a computer to transmit results — see Section 11 for instructions. In the Home screen, press the Recall icon. Press Transmit All. The analyzer displays “Transmitting All...”...
  • Page 90 6-10 Recalling Results...
  • Page 91: Section 7: Calibration And Quality Control

    Section 7 Quality Control Calibration The VetScan VS2 Analyzer is self-calibrating. A bar code on the reagent rotor contains the information needed to calibrate the rotor when run. Additionally, each reagent bead used in the rotor is calibrated to a refer- ence method and/or reference material.
  • Page 92 Quality Control During Analysis During analysis, the analyzer checks its internal components and the reagent rotor to ensure the accuracy of the results. Analyzer Before analysis begins, the analyzer’s photometer takes readings with the light path obstructed and unobstructed to determine the appropriate light intensity range, and then checks that the range is within specification.
  • Page 93: Running Controls

    Running Controls A control is a biological sample or solution with a suitably contrived matrix that is analyzed for purposes of quality control. The composition of the matrix must be such that the solution closely matches that of the biological specimen for characteristics of importance to the analyzer. Control materials need to be stable and available in sufficient volumes in multiple portions and over an extended period.
  • Page 94 In the Home screen, press ANALYZE to open the rotor drawer. Insert the control sample into the reagent rotor (see “Running Tests” on page 4-5), and place the rotor into the drawer. Press CLOSE to close the drawer and start analysis. Enter the Operator ID using the touchscreen, then press Enter Operator ID Done.
  • Page 95 Enter an alternate ID (up to 14 characters), then press Done. The analyzer checks the rotor type, then begins processing the sample with no further input. The analyzer indicates when the sample is finished processing, then automatically prints the results of the analysis. If the results do not print automatically, they can be recalled from memory and printed —...
  • Page 96 Calibration and Quality Control...
  • Page 97: Section 8: Troubleshooting

    If the troubleshooting recommendations contained in this section do not correct the problem with the analyzer or the reagent rotors, record the error message and/or print an error report and contact Abaxis Technical Service, 24 hours a day, 7 days a week, at 1-800-822-2947, or send a fax to 1-877-900-9333.
  • Page 98: Instrument And Result Messages

    “flags” — “HEM”, “LIP”, “ICT”, or the symbols “~~~” — in place of values. Contact Abaxis Technical Service at 1-800-822-2947 to review the patients results printed on the error report. You can print the error report using the Recall function starting from the Home screen.
  • Page 99: Out-Of-Range Samples

    A sample that is found to be out-of-range more than once should be tested by an alternate laboratory-approved method, or sent to a referral laboratory. Rotor Cancellations and Procedures If the rotor cancels, record the following information or print an error report, and contact Abaxis Technical Service at 1-800-822-2947: Lot number...
  • Page 100: Sample Dilution

    Sample Dilution The following customer-developed protocol can be used for diluting samples. Dilution of Sample 1:1 with Saline (Use serum or heparinized plasma) Combine one part (100 L) serum or heparinized plasma with one part (100 L) normal saline (0.9%) (do not use distilled water) into a clean, red top tube and gently mix. Use the diluted sample for testing.
  • Page 101 Note: The next step is irreversible. All settings — including custom reference ranges and the date and time —are returned to their factory values, and the memory is cleared. Results previously stored, however, are not affected. Press CONTINUE to restore all factory settings (including the clock) and clear all memory from the analyzer.
  • Page 102 Troubleshooting...
  • Page 103: Section 9: Principles Of Procedure And Operation

    The reagent rotor contains a diluent, and test-specific reagent beads. The operator then places the rotor in the VetScan VS2 Chemistry Analyzer and enters the appropriate identification numbers. The VS2 Analyzer automatically performs the remainder of the testing protocol.
  • Page 104: Principles Of Operation

    Principles of Operation Chemical reactions occur between reagent beads, the diluent contained in the reagent rotor, and the added sample. These reactions produce chromophores that are measured photometrically by the VS2. The microprocessor then calculates the concentrations of the analytes. The measurement optics include a stroboscopic xenon lamp, a wavelength selection system, and a multiple-wavelength detector.
  • Page 105: Rate Methods

    The equation for calculating absorbance is this: (I λ1RC - I λ1 ) / (I λ2RC - I λ2 A = -log (I λ1OC - I λ1 ) / (I λ2OC - I λ2 where: = Absorbance at wavelength 1, referenced to wavelength 2 = Intensity of light transmitted through the reaction cuvette at wavelength 1 = Intensity of light transmitted through the reaction cuvette at wavelength 2 = Intensity of light transmitted through the dark cuvette at wavelength 1...
  • Page 106: Endpoint Reactions

    Endpoint Reactions Reagents, samples, and chromophores all absorb light in endpoint reactions. Light absorption by the samples must be subtracted so that the analyte concentration can be isolated. The absorbance of the sample is measured in a sample blank cuvette. Some chemistries employ a generic sample blank and others (such as total bilirubin) use a dedicated sample blank.
  • Page 107: Section 10: Maintenance And Service

    Maintenance and Service Section 10 The VetScan VS2 Analyzer requires minimal maintenance. Clean the exterior of the analyzer weekly with mild detergent and a soft, damp cloth. The air filter requires cleaning once a month. Regular mainte- nance of the analyzer will assure reliable operation.
  • Page 108: Cleaning Spills

    10-3 see page 10-4 Note: For replacement filters, contact Abaxis Customer Service (1-800-822-2947, during regular business hours). 1. National Committee for Clinical Laboratory Standards (now Clinical and Laboratory Standards Institute). 1992. Physician’s office lab oratory guidelines, 2nd ed; tentative guideline. NCCLS document POL1-T2 (ISBN 1-56238-159-8).
  • Page 109: Changing An Air Filter With A Cover

    Changing an Air Filter With a Cover Turn the analyzer off by pressing and holding the Power button. Remove the power cord from the back of the analyzer. Remove the filter cover from the back of the analyzer. Remove the filter. Wash the filter with warm water to remove dust, animal hair, and any other debris.
  • Page 110: Changing An Air Filter Without A Cover

    Changing an Air Filter Without a Cover Turn the analyzer off by pressing and holding the Power button. Remove the power cord from the back of the analyzer. Remove the black mesh filter from the circular fan vent. Wash the filter with warm soapy water to remove dust, animal hair, and any other debris.
  • Page 111: Updating The Analyzer Software

    10.4 Updating the Analyzer Software Abaxis provides software updates on CD-ROM to registered owners of the VetScan VS2 Ana- lyzer. Replacement software is mailed immediately whenever a new software version is released. See Section 5.6, “Viewing Analyzer Identification” to determine the software version installed in the analyzer.
  • Page 112: Returning The Analyzer For Service

    Authorization for service to the analyzer must be obtained from Abaxis Technical Service before sending the analyzer in. To obtain authorization for service on the analyzer, call Abaxis Technical Service, 24 hours a day, 7 days a week, at 1-800-822-2947.
  • Page 113: Section 11: Connecting To An External Computer

    Connecting to an Section 11 External Computer The VetScan VS2 Analyzer is equipped to transmit patient and control results in 8-bit, ASCII (American Standard Code for Information Inter- change) format to a computer through a USB port. To receive the results from the VS2, the computer requires terminal emulation software, such as HyperTerminal, which is included with Windows 95 and higher.
  • Page 114: Transmission Specifications

    11.2 Enabling the VetScan VS2 Communication Protocol The VetScan VS2 can communicate with computers running Microsoft Windows (2000, XP, or Vista), Macintosh OS X, or Linux (kernel 2.4 or above). To enable this communication, you must set the VS2’s communication protocol as follows: Follow the procedure given in steps 1 through 3 of “Setting Communication Protocol”...
  • Page 115: Installing The Windows 2000/Xp Driver

    11.3 Installing the Windows 2000/XP Driver Use this procedure to install the Abaxis driver that enables the analyzer to communicate with a PC running Windows 2000 or XP. The analyzer installs on the PC as a virtual communication port in Windows.
  • Page 116 Click Start > Control Panel > System, then click the Hardware tab. Click Device Manager, then click the “+” before Ports (COM & LPT). Look for “Abaxis Blood Chemistry Analyzer.” Note: If the analyzer does not appear in the device list, do not reinstall the driver.
  • Page 117 Note the port number to which the analyzer is assigned, and record this number. You will use this port number later in this procedure. (In the example at right, the port is COM4.) Close the Device Manager. Click Start > Run, and in the dialog enter hypertrm.
  • Page 118 In the COM Properties dialog, use the Bits per second pull- down menu to select 9600. Transmit patient results from the VS2 patient archive. For instructions, see “Transmitting Results” on page 6-9. Retransmit results from the VS2: In the Home screen, press the Recall icon. Press Last Rotor.
  • Page 119: Installing The Windows Vista Driver

    11.4 Installing the Windows Vista Driver Use this procedure to install the Abaxis driver that enables the analyzer to communicate with a PC running Windows Vista. The analyzer installs on the PC as a virtual communication port in Win- dows.
  • Page 120 Some of these screens may vary slightly on your system. Install the driver as follows. Insert the Abaxis Driver CD (software files: P/N 110-5001) into the computer’s CD drive. The computer’s default web browser then opens, displaying the VS2 driver installation guide links.
  • Page 121 Use the USB cable to connect the VS2 to any available USB port on the computer. Click Start > Control Panel > Hardware and Sound > Device Manager. Click the “+” before Ports (COM & LPT). Look for “Abaxis Blood Chemistry Ana- lyzer.”...
  • Page 122 Transmit patient results from the VS2 patient archive. For instructions, see “Transmitting Results” on page 6-9. Retransmit results from the VS2. In the Home screen, press the Recall icon. Press Last Rotor. The results from the last rotor are displayed. Press Print.
  • Page 123: Installing The Mac Os X Driver

    11.5 Installing the Mac OS X Driver Use this procedure to install the Abaxis driver that enables the Abaxis analyzer to communicate with a Mac. The analyzer installs on the Mac as a virtual communication port in Mac OS X.
  • Page 124 Click Restart. Connect the USB cable to a Type A connector on the Mac and to the uppermost USB con- nector (Type B) on the back of the analyzer. Turn on the analyzer. Use the Network section in the System Preferences application (Go >...
  • Page 125: Installing The Linux Driver

    11.6 Installing the Linux Driver Use this procedure to install the Abaxis driver that enables the Abaxis analyzer to communicate with a PC running Linux. The analyzer installs on the PC as a virtual communication port. Installation requires the following: VetScan VS2 Analyzer PC with Linux (kernel version 2.4 and above) and a USB port...
  • Page 126: Using Hyperterminal

    If HyperTerminal does not appear, install it by going to Start > Settings > Control Panel > Add/Remove Programs (Add/Remove Windows Components > Communica- tions tab). Consult Windows help for details. Type a descriptive name (such as Abaxis) for the connection in the Name box. Click OK. 11-14...
  • Page 127 In the Connect Using dialog, select the port that the ana- lyzer is connected to, then click OK. (The other settings are not required.) Note: If the port is unknown, select any one for now. If needed, you can run the wizard again to try other ports.
  • Page 128: Capturing And Transmitting Results

    Capturing and Transmitting Results Prepare HyperTerminal to capture results by loading a saved session file or by creating a new session file as described in Section 11.7. Click Transfer > Capture Text on the menu. Select a path and file name and click Start. Any results transmitted to the computer from this point forward will be captured in the designated text file.
  • Page 129: Section 12: Ordering Information

    Ordering Information Section 12 12.1 Required Materials This section lists the materials needed to operate the VetScan VS2 according to recommended procedures — see Section 4, “Test Proce- dure and Results.” 12.2 Ordering Information To order reagent rotors, pipettes, tips, printer paper, or other VetScan...
  • Page 130: Vetscan Reagent Rotors

    12.3 VetScan Reagent Rotors Reagent Rotor Package Catalog Number Comprehensive Diagnostic Profile Abaxis # 500-0038 Prep Profile II Abaxis # 500-0026 Critical Care Plus Abaxis # 500-0042 Equine Profile Plus Abaxis # 500-0043 Large Animal Profile Abaxis # 500-0023 /Cholesterol Profile...
  • Page 131: Section A: Iqc Codes

    If the iQC code is not listed or if you cannot correct a particular problem, record the iQC number or print the report, and contact Abaxis Technical Service, 24 hours a day, 7 days a week, at 1-800-822-2947, or send a fax to 1-877-900-9333.
  • Page 132 If the iQC code is not listed or if you cannot correct a particular problem, record the iQC num- ber or print the report, and contact Abaxis Technical Service, 24 hours a day, 7 days a week, at 1-800-822-2947, or send a fax to 1-877-900-9333.
  • Page 133 If the iQC code is not listed or if you cannot correct a particular problem, record the iQC num- ber or print the report, and contact Abaxis Technical Service, 24 hours a day, 7 days a week, at 1-800-822-2947, or send a fax to 1-877-900-9333.
  • Page 134 If the iQC code is not listed or if you cannot correct a particular problem, record the iQC num- ber or print the report, and contact Abaxis Technical Service, 24 hours a day, 7 days a week, at 1-800-822-2947, or send a fax to 1-877-900-9333.
  • Page 135: Section B: Supported Printers

    Supported Printers Appendix B Printers Supported by the VetScan VS2 This section lists the printers supported by the VetScan VS2 as of November, 2008. Note: Use only printers that are supported by Abaxis oper- ating systems. Abaxis has validated the following...
  • Page 136 Officejet 3XX, 5XX, 6XX, 7XX, 11XX Officejet 51XX, 61XX, 62XX, 63XX, 4100, 4105, 4200, 5500, 9100 Officejet 71XX, 72XX, 73XX, 74XX, 9X11 Officejet d Series Officejet G Series Officejet J Series Officejet K Series Officejet Lx Officejet R Series Officejet t Series, v Series Officejet Pro K Series, Pro L Series PSC 500, 7XX, 9XX, 15XX, 16XX PSC 21XX, 22XX, 23XX, 24XX, 25XX...
  • Page 137: Section C: Methods Performance

    Methods Performance Appendix C C.1 Materials Required The following materials are required to operate the VetScan VS2 accord- ing to the recommended procedure given in Section 4. Materials Catalog Number Provided with Purchase of Reagent Rotor Profiles VetScan Reagent Profile...
  • Page 138 Rotor Test Panels The following chart shows the test panels provided by each profile rotor. Methods Performance...
  • Page 139 Warnings and Precautions Indications of Reagent Rotor Instability or Deterioration • The diluent container in the reagent rotor is automati- cally opened when the analyzer drawer closes. A rotor • When stored as described above, all reagents con- with an opened diluent container cannot be re-used. tained in the reagent rotor are stable until the expira- Ensure that the sample or control has been placed into tion date printed on the rotor pouch.
  • Page 140 • Glucose concentrations are affected by the length of time since the patient has last eaten and by the type of sample collected from the patient. To accurately inter- The VetScan VS2 Chemistry Analyzer operates at ambi- ° ° pret glucose results, samples should be obtained from ent temperatures between 15–32...
  • Page 141 • Lithium heparin is the only anticoagulant recom- See Section 7.3, “Running Controls” to run controls. mended for use with the VetScan VS2 Chemistry Ana- lyzer. C.12 Calibration • If a result for a particular test exceeds the assay range, The VetScan VS2 Chemistry Analyzer is calibrated by the the sample may be able to be diluted (see Section 8).
  • Page 142 Ranges cited by Meyer, et al for dogs and cats were adjusted to reflect field data collected by Abaxis on the VetScan VS2 Chemistry Analyzer. Cutoffs for Bile Acids (BA) set for 100% specificity with a sensitivity of 74% for dogs and cats. See Ettinger SJ, Feldman EC, eds. St. Louis: W.B.
  • Page 143 C.17 Performance Characteristics Linearity The chemistry for each analyte is linear over the dynamic range listed in Table 2 when the VetScan VS2 Analyzer is operated according to the recommended procedure (see Section 4). Table 2: VetScan VS2 Dynamic Ranges...
  • Page 144 Table 3: Correlation of VetScan System with Comparative Method Canine Feline Equine Bovine Analyte (n = 22–180) (n = 20–55) (n = 7–101) (n = 126) Alanine Aminotransferase (ALT) (U/L) Correlation (r) 1.00 0.98 0.97 Slope 0.95 0.92 0.94 Intercept Sample range 10–1549 27–99...
  • Page 145 Table 3: Correlation of VetScan System with Comparative Method (Continued) Canine Feline Equine Bovine Analyte (n = 22–180) (n = 20–55) (n = 7–101) (n = 126) Creatinine (CRE) (mg/dL) Correlation (r) 0.99 1.00 0.95 Slope 1.00 1.01 1.00 Intercept -0.1 -0.4 Sample range...
  • Page 146 C-10 Methods Performance...
  • Page 147: Index

    Index Diluent Container 3-8 Direct Current Symbol 2-2 Absorbance 9-3 Advanced Functions 4-5 Endpoint Reactions 9-4 Setting 5-30 Error Messages C-5 Air Filter Expected Values C-6 Changing 10-3, 10-4 With Cover 10-3 Without Cover 10-4 Alert Fasting Patients 4-14 Volume 5-22, 5-23, 5-26 Foil Pouch 4-6 Opening 4-6 Bar Code 3-8...
  • Page 148 Maintenance 10-1 Quality Control 7-1, C-5 Cleaning 10-1 During Analysis 7-2 Air Filter 10-2 Analyzer 7-2 Exterior 10-1 Reagent Rotor 7-2 Spills 10-2 Sample 7-2 Normal Range 4-13, C-5 Rate Methods 9-3 Reagent Rotors 3-8, 4-15, C-2 Bar Code 3-8 Bar Code Ring 3-8 Operation 9-1 Cuvettes 3-8...
  • Page 149 Start Button 3-7 Touchscreen 3-7 Supplies Transmitting All Error Records 6-9 Ordering 1-2, 12-1, 12-2 Troubleshooting 8-1 Supported Printers A-1, B-1 Abaxis Technical Service 8-1 Symbols Chemistry Suppression 8-2 Biohazard 2-2 Error Report, Printing 8-2 Caution 2-2 Messages 8-1 Direct Current 2-2...
  • Page 150 SI (Systeme International) 5-16 Connecting to an External Computer USB Connection 11-1 Symbol 2-2 USB Connections 3-6 USB Connections 3-6 VetScan VS2 Operator’s Manual 3-1 USB Ports 2-1 Warranty Card 3-1 Weight 3-5 Volume VetScan VS2 Chemistry Analyzer Setting 5-22, 5-23, 5-26...

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