Thermo BioMate 3 series Operator's Manual page 77

Spectrophotometers
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The following table lists all the parameters used for the BioMate 3 applications and for the general tests
available on the instrument and a brief description of each parameter.
Parameter
# of Wavelengths
# of wavelengths for
wavelength accuracy
# Phot Accy Standards
%Transmittance
% of lamp life used
%Formamide
%GC
Absorbance
Auto Print
Base Sequence
Beeper
Cell Correction
Cell Pos #
Concentration
Correction Mode
Cursor position on
Test Types menus
Cursor position on
Utility menu
Curve Fit
Date Standards Measured
Date/Time Setup
Delay Time
Diluent Volume
Dilution Multiplier
Display Activity
Display Protein
DNA ε(260)
DNA Mol. Wt.
Factor
Factor 1
Factor 2
Number of wavelengths set for multiple wavelength analysis
Used to determine wavelength accuracy of unit
Used to determine photometric accuracy of unit
Sets of transmittance value
Estimated percentage of lamp life used (based on typical lamp life of 5 years)
Percentage of formamide contained in the sample
Percentage of GC pairs contained in the sample
Set absorbance value
Turns automatic printout on or off
Sequence of bases contained in the sample
Turns audible signal on and off
Program that automatically corrects for variances in absorption between the
cuvetts used to hold and measure samples
Position placed in light path; set by selection of sample positioner
Sets concentration value
Mode for cell correction collection
Indicates which option on the Test Types screen should be highlighted
Indicates which option on the Utility screen should be highlighted
Type of Curve fit calculation
Date when standards were last measured with this instrument
Current date and time settings for the instrument
Time from Test Initiation to first measurement; allows for sample equilibration
Volume of diluent added prior to measurement
Factor used to correct for sample dilution
Indicates whether results should appear as units of enzyme activity
Indicates whether results should include protein concentration
Extinction coefficient
Molecular weight of DNA contained in sample
Abs x Factor = Concentration
Abs/min x Factor = Result
Abs(WL1) x Factor = Result
Abs(WL2) x Factor = Result
B-1
Appendix B - Parameters
Description
BioMate 3 Operator's Manual

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