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Fein Optic IRB20 User Manual

Inverted microscope user’s manual
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IRB20 Inverted Microscope User's Manual
Fein
info@feinoptic.com

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Summary of Contents for Fein Optic IRB20

  • Page 1 IRB20 Inverted Microscope User’s Manual Fein info@feinoptic.com...
  • Page 2 IRB20 Microscope Components Lamp Housing C-Mount Adapter Phase Slider Eyepieces Condenser Mechanical Stage Siedentopf Trinocular Head Beam Splitter On/Off Power Stage X-Y Control Rheostat Nosepiece Coarse Focus Fine Focus Fein...
  • Page 3 IRB20 Microscope Components 1. Eyepiece 12. Nosepiece 2. Siedentopf Trinocular Head 13. Power Socket 3. Phase Contrast Slider 14. Fuse Box 4. Aperture Diaphragm Lever 15. Power Switch 5. Filter Slider 16. Surplus Water Plate 6. Lamp Housing 17. Mechanical Stage X-Y Knob 7.
  • Page 4 IRB20 Inverted Microscope Assembly • Once the microscope is assembled, turn on the main power source. • Place the sample on the stage. • Move the 10x objective lens into the light path. • Focus on the sample using the coarse focus and then the fine focus.
  • Page 5 IRB20 Inverted Microscope Operation Instructions Do not shake or drop the microscope. Do not expose the microscope to direct sun, high temperatures, high humidity, dust, or damp environments. Use a flat work surface. When moving the microscope use both hands, holding the handle at the back (1) and the frame at the front (2) as shown in the image at right.
  • Page 6 IRB20 Inverted Microscope Operation Instructions Place the filter (1) inside the filter holder (2), and insert into the filter slot on the condenser. The tension of the coarse focusing knob can be adjusted by holding the tension adjustment ring (1) and rotating it.

  • Page 7: Mechanical Stage

    IRB20 Inverted Microscope Operation Instructions The eyepieces can be locked in place with a set screw (1). To change the eyepiece, loosen the set screw and pull the eyepiece out of the eyetube. FIXED STAGE: Insert the round plate (1) into the fixed stage.
  • Page 8 IRB20 Inverted Microscope Bulb Replacement Turn off and unplug the microscope prior to changing the bulb. 1. Remove the screw with the allen wrench. 2. Lift the illumination cover. 3. The bulb is located at the front of the housing.

  • Page 9: Fuse Replacement

    IRB20 Inverted Microscope Bulb & Fuse Replacement When you lift the bulb housing be careful since the wires are still connected. In order to replace the bulb remove the solder joints from the wires connected to the bulb. Unscrew the 2 screws holding the bulb to the housing.
  • Page 10 IRB20 Inverted Microscope Phase Contrast Setup Ensure phase slider is right side up and slide into the condenser. Place phase objective in the light path and put the phase slider in the position that matches the phase objective magnification. Remove the eyepiece and insert the phase centering telescope. Use the phase centering screws to align the bright and dark rings as shown in the illustrations below.
  • Page 11 IRB20 Inverted Microscope Trouble Shooting TROUBLE POSSIBLE CAUSE SOLUTION Nosepiece is not clicked into position. Move nosepiece into position. Field of view obstructed or Filter slider is not in place. Adjust filter slider. irregular illumination Phase Contrast slider is not in place.