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Summary of Contents for Fein Optic RB50
- Page 1 RB50 Microscope User’s Manual info@feinoptic.com...
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Page 2: Microscope Components
Microscope Components: RB50 Fluorescence Microscope Trinocular Port Eyepieces Beam Splitter Field Diaphragm Adjustment Aperture Diaphragm Adjustment Filter Cube Hex Wrench Selector Diaphragm Centering Screws Shutter Lever Y-Axis Knob Sextuple Nosepiece X-Axis Knob Mechanical Stage Condenser Voltage Indicator Reset Button for Intensity... - Page 3 Microscope Components: RB50 Fluorescence Microscope Fluorescence Illuminator ND Filter Slots Attachment Quintuple Nosepiece 100w Halogen Light Source Coarse Focus Knob Coarse Adjustment Limit Ring Fine Focus Knob Filter Levers Page 2 info@feinoptic.com...
- Page 4 Binocular / Trinocular Microscope Assembly: RB50 Microscope Assembly RB50 Fluorescence Microscope Assembly Page 3 info@feinoptic.com...
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Page 5: Before Use
Before Use: Do not shake or drop the microscope. Do not not expose the microscope to direct sun, high temperatures, dust, or damp environments. Use a at work surface. Indoor operating temp 41°~104°F (5°~40°C), max relative humidity of 80%. When moving the microscope use both hands, holding by the handle at the back (1) and the base (2) as shown at right. -
Page 6: Maintenance
Do not disassemble the microscope. After use, cover the microscope with a dust cover and power o the light. Objectives: RB50 In nity Corrected Objectives all have a parfocal distance of 45mm. Recommended coverslip thickness is 0.17mm. Numerical Aperture Working Distance... - Page 7 Step-by-Step Assembly: Light Source Assembly & Bulb Replacement: Loosen the lock screw (1) completely with an M4 spanner and remove the cover. Open the bulb lock (2). Handle the bulb (3) with a clean glove or soft tissue (do not touch the bulb with ngers). Insert the bulb pins (4) into the bulb holder (5).
- Page 8 Step-by-Step Assembly: Condenser Assembly: Rotate the coarse focusing knob (1) to raise the stage to its highest setting. Rotate the condenser height adjustment knob (2) to lower the condenser bracket to its lowest setting. Loosen the condenser set screw (3). Swing out the front lens of the condenser with the scale facing forward.
- Page 9 (2) on the back of the microscope. Insert one end of the power cord (3) into the power socket (4). Plug in the microscope. Use only the power cord supplied by Fein Optic. Illumination: Turn the main power switch to “--” on.
- Page 10 Step-by-Step Assembly: Slide Placement: Push the slide holder clamp (1) backwards. Place the slide between the slide holder clamp (1) and the slide holder (2) with the cover slip facing up. Rotate the X-Axis knob (4) and the Y-Axis knob (3) to position the slide in the center under the objective.
- Page 11 Step-by-Step Assembly: Focus Tension Adjustment: If the focus knob is very tight or the stage drifts after being focused, the tension adjustment knob (1) should be adjusted. Rotating the knob clockwise will tighten the tension and rotating it counterclockwise will loosen the tension of the focus knob. Interpupillary Distance Adjustment: When using both eyepieces for observation, hold the base of the eyetubes and rotate the eyepieces...
- Page 12 Step-by-Step Assembly: Stage Adjustment: When looking through the microscope, move the stage by rotating the X-Axis adjustment knob (1) and the Y-Axis adjustment knob (2). The movement range of the X-Axis and Y-Axis is 80 x 55mm. X-Axis & Y-Axis Knob Adjustment: Hold the X-Axis knob (1), tighten the Y-Axis knob (2) to expose the adjustment knob.
- Page 13 Step-by-Step Assembly: Centering the Condenser: Rotate the condenser raise/lower knob (1) to raise the condenser up to the highest position. Rotate the spanner (2) on the condenser to move the front lens into the light path. Use this condenser lens in the light path when the objective is 20x or higher.
- Page 14 Step-by-Step Assembly: Using Color Filters: A color lter can make the background light more suitable and strengthen the image contrast. When an external color lter is used, place a 45mm diameter lter into the groove on top of the illuminator (1). Place the rough side of the lter facing down.
- Page 15 Fluorescence Assembly: Fluorescence Illuminator Assembly: Remove the caps (1) from the illuminator. Place the illuminator on the microscope body then slide it toward the light source to make the epi-illuminator at with the body. Tighten the four M5 hex screws in the illuminator with the spanner and replace the caps (1).
- Page 16 Fluorescence Assembly: Nosepiece Assembly: Loosen the set screw on the arm (1). Match the dovetail interface (2) of the nosepiece with the dovetail groove of the arm. Push the nosepiece into the slot. Tighten the set screw (1). Fluorescence Light Source Assembly: Loosen the set screw (1) on the uorescence illuminator.
- Page 17 Fluorescence Assembly: Centering the Field Diaphragm: By adjusting the eld diaphragm, the beam of light is adjusted according to the objective and will create sharper image contrast. To prevent a uorescence decrease, narrow the eld diaphragm and reduce the illuminated part of the sample.
- Page 18 Fluorescence Assembly: Centering the Aperture Diaphragm: Rotate the turntable to place the uorescence lter B or G into the light path. (If there is no uorescence lter B or G use another). Rotate the 10x objective into the light path. Put the shutter (1) to position “O”...
- Page 19 Fluorescence Assembly: Installing Filters: Image contrast and background light can be adjusted by using lters. Insert desired lter or ND lter into position (1) and position (2) on the side of the microscope. The lter iden cation side of the lter should face the observer when inserting the lter into the microscope.
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Page 20: Troubleshooting
Use the same eyepieces in each Di erent eyepieces are being used in the match with the left. eyetube. Use the Fein Optic left and right eyetube. FPL-WF10x/22 Eyepieces. Page 19 info@feinoptic.com... - Page 21 Troubleshooting: Mechanical & Electrical Troubleshooting Problem Cause Solution The cover glass is not facing up. Put the cover glass face up. Can not get the objective lens to focus. Use cover glass thickness The cover glass is not standard thickness. 0.17mm.