BIO RAD iCycler iQ Packing List And Installation Manual page 22

Before capturing an image.
DC offset too high.
Black line is too close
to White line.
7. Once the DC offset is correct, take another exposure at 640 ms and check for saturated
pixels. As you move the cursor over the image, the X and Y coordinates are displayed at
the top of the image as well as the pixel intensity (labeled I). Move the cursor over the
brightest pixels on the image and make sure that the intensity is not more than 1000 counts.
If any pixels are saturated, then reduce the exposure time to 320 ms and check again for
saturated pixels. If you still have saturated pixels, then you must use a more dilute solution
of the fluorescein in the calibration plate.
8. Once you can collect an image at 640 or 320 ms without saturated pixels, double click on
the Exp Count field in the Exposure dialog box and enter 10. Click Expose to collect and
display the average of 10 consecutive exposures.
9. When the image is displayed, go to the Tools menu and select Factor Generator.
10. The "Select Source Mask File" dialog box will open. Select the file
c:\Program Files\Bio-Rad\iCycler\Ini\Mask96.ini
and click Open.
White line
Black line
DC offset too low.
Black line cuts peak
too early.
20
DC offset correct.