BIO RAD iCycler iQ Packing List And Installation Manual page 18

3. To adjust the masks over all of the plate wells at one time, use the Global buttons in the
Mask Editor dialog box. Select the Show All button. Masks will appear over each of the
wells of the calibration plate (see Figure 6.4). The masks may be moved as a group, Left,
Right, Up, and Down, by selecting each of those buttons. Adjust the mask position until
every well is approximately centered within an inner square. (Note: When the full mask
is displayed, the inner boundary of most of the wells is discernable; the outer boundary
appears distorted because of the overlap between adjacent outer boundaries.)
4. Under Global, click the Auto Position button. The program will automatically center the
masks over each of the 96 wells then display the results. For best results, click Auto
Position again. (Note: To check the position of any individual well in the Mask Editor
dialog box select the Show One button, then select the up and down arrows in the
Column and Row text boxes to move the mask to the different wells. You can adjust the
position of an individual wells by using the up and down arrows in the X-position and
Y-position boxes. Alternatively you can drag the mask to its new position. There is also
an auto adjust feature for individual wells.)
5. When finished, click Save in the Mask Editor dialog box. This automatically saves the
changes to the Mask; the Mask Editor dialog box will disappear. Select the Cancel
button to escape without saving the changes.
Fig. 6.4. iQ configuration window with masks centered over the 96 wells of the calibration plate.
Note that not all of the inner and outer boundaries are displayed on the screen.
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