BIO RAD iCycler iQ Packing List And Installation Manual page 11

1. Turn off the power to the Optical Module.
2. Release the two black latches on each side of the Optical Module. Slide the housing
backwards 2–3" (5–8 cm), exposing a black case, the filter wheel housing. It is not
necessary to remove the housing or cables.
3. Remove the two rubber plugs on the top of the filter wheel housing by pulling them
straight upward. These plugs shield the filter wheels. The excitation filters are located in
the slot on the right side of the instrument; the emission filters are located in the slot in the
center of the instrument (see Figure 5.2). The tops of the filter blanks in position 1 (the
"home" position) should be visible in the slots containing both the excitation and
emission filters; the number "1" should be visible in the slot on each side of both filters.
Changing both types of filters is similar.
4. Turn the filter wheels to the desired positions using the ball end hex driver. As long as the
power to the Optical Module is off, the filter wheels may be turned freely in either direction.
5. To remove a filter, grasp it on both sides with the filter removal pliers and squeeze the tab
in; gently pull the filter up and out.
6. To insert a filter, grasp the filter with the pliers and insert it into a vacant slot. For the
excitation filters, the tab on the filter is toward the front of the instrument. For the
emission filters, the tab on the filter is toward the right of the instrument. Be sure that
every position in the filter wheel has either an excitation or emission filter or a filter blank.
Record the position of filters to compare later with the plate setup. (See Section 4.2
and Section 7.1.)
7. After the filters or filter blanks have been inserted, replace the rubber plugs over the slots
of the filter wheels.
8. Move the camera housing forward and re-attach the latches.
Fig. 4.2. Installing the filters.
Emission
Filter Wheel
Slot with Excitation
Filter Wheel
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