(5.1) IRGA CO
readings are corrected for water vapor, temperature, and atmospheric pressure. The
2
addition of transpirational water vapor dilutes the air leaving the cuvette (C
for in the calculation:
(5.2) To calculate net CO2 assimilation we rearrange equation (5.1) to:
CIRAS-3 calculates and displays the CO
CIRAS-3 display:
Intercellular CO
2
Calculate CO
concentration in the sub-stomatal cavity (C
2
& Farquhar, 1981
(6.1) The sub-stomatal CO2 concentration, C
(6.2) Where g
is the total conductance to CO
c
(1.585 is the diffusion ratio of CO
boundary layer).
Note: These calculations are based on the following assumptions:
·
the leaf is exposed on both upper and lower leaf surfaces
·
the upper and lower boundary layer resistances are similar
·
stomata are evenly distributed on both upper and lower leaf surfaces.
CIRAS-3 Operation Manual V. 1.09
= (
= −�� (
difference (C
2
= (
Concentration
�
�
� = �
−
−
and water in air, and 1.37 is the diffusion ratio of CO
2
× ) − [
× ( + )]
−
) × � + (
- C
). As relates to the calculated values in the
out
in
) =
+
) using the equation derived by von Caemmerer
i
, is given by:
i
−
� ×
��
� =
−
�
+
transfer:
2
( . ×
) + ( . ×
162
), and this is compensated
out
× ) �
� −
�
� ×
)
and water in the
2
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