Related Manuals for Implen NP80
Summary of Contents for Implen NP80
- Page 1 NanoPhotometer NP80/N60/N50/C40 User Manual Version 1.3.2 Software Version 1.1.11648...
- Page 2 NanoPhotometer NP80/N60/N50/C40 User Guide Version 1.3.2 Declaration of conformity for the NanoPhotometer (C40/N50/N60/NP80) This is to certify that the Implen NanoPhotometer conforms to the requirements of the following directives: 2014/35/EU Low Voltage Equipment Safety Directive 2004/108/EC EMC Directive IEC 60529...
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Page 3: Table Of Contents
NanoPhotometer NP80/N60/N50/C40 User Guide Version 1.3.2 Contents ................. 6 HOTOMETER AT A LANCE ....................6 HOTOMETER VERVIEW NP80 ........................7 HOTOMETER N60 ........................8 HOTOMETER N50 ........................9 HOTOMETER C40 ........................10 HOTOMETER (NP80/N60/N50/C40) ................11 NSTRUMENT ANEL (NP80/N60/N50/C40) ................11... - Page 4 NanoPhotometer NP80/N60/N50/C40 User Guide Version 1.3.2 ........................38 UCLEIC CIDS .......................... 38 ETHOD VERVIEW ....................... 38 EASUREMENT ROTOCOL ..........................40 ALCULATIONS ........................45 ROTEIN .......................... 45 ETHOD VERVIEW ....................... 46 EASUREMENT ROTOCOL ..........................47 ALCULATIONS ....................... 51 ROTEIN SSAYS .......................... 51...
- Page 5 NanoPhotometer NP80/N60/N50/C40 User Guide Version 1.3.2 ..........................68 ALCULATIONS ........................68 USTOM ......................68 TORED ESULTS ......................69 TORED ETHODS ....................69 REFERENCES ..........................69 ENERAL ..........................69 ATE AND ............................70 ISPLAY ............................. 70 BOUT ......................... 70 CCESS OINT .......................
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Page 6: Nano Photometer At A Glance
The N50, N60, and NP80 spectrophotometers measure sample volumes ranging from 0.3 - 2 µl. The NP80 model also has the capability to measure samples using a standard cuvette. The C40 is a mobile standard cuvette solution with the option to upgrade to accommodate nanovolume measurements. -
Page 7: Nano Photometer Np80
NanoPhotometer NP80/N60/N50/C40 User Guide Version 1.3.2 NP80 HOTOMETER Item Number Touchscreen Battery Pack NP80 NP80-Touch NP80-Mobile... -
Page 8: Nano Photometer N60
NanoPhotometer NP80/N60/N50/C40 User Guide Version 1.3.2 HOTOMETER Item Number Touchscreen Battery Pack N60-Touch N60-Mobile... -
Page 9: Nano Photometer N50
NanoPhotometer NP80/N60/N50/C40 User Guide Version 1.3.2 HOTOMETER Item Number Touchscreen Battery Pack N50-Touch... -
Page 10: Nano Photometer C40
NanoPhotometer NP80/N60/N50/C40 User Guide Version 1.3.2 HOTOMETER Item Number Touchscreen Battery Pack C40-Touch C40-Mobile... -
Page 11: Instrument Rear Panel (Np80/N60/N50/C40)
NanoPhotometer NP80/N60/N50/C40 User Guide Version 1.3.2 (NP80/N60/N50/C40) NSTRUMENT ANEL (NP80/N60/N50/C40) NSTRUMENT OTTOM Model name, device serial number and FCC ID are located on the identification plate on the bottom of the instrument. -
Page 12: Accessories
Note: Use only the power adapter, supplied with your instrument or a replacement part from the manufacturer or your supplier. Dust Cover PTIONAL CCESSORIES Didymium Glass Filter (C40/NP80) The certified didymium glass filter is used as a control of wavelength and photometric accuracy for the NanoPhotometer cuvette applications. - Page 13 340 - 950 nm. Field Kit Implen aluminum roller case for safe transportation, designed to fit in most airplane overhead bins; special compartments for all necessary accessories, cleaning tools and samples. Note: Delivered w/o NanoPhotometer and accessories Note: Only operate the NanoPhotometer in the roller case when cover is open.
- Page 14 NanoPhotometer NP80/N60/N50/C40 User Guide Version 1.3.2 Dymo Label Printer It is possible to connect a Dymo Label printer to the NanoPhotometer for direct printout on sticky labels. Recommended and tested printers are the Dymo LabelWriter 4XL (label size 10.3 x 15.8 cm) and the Dymo LabelWriter 450 (5.4 x 10 cm).
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Page 15: Connectivity
NanoPhotometer NP80/N60/N50/C40 User Guide Version 1.3.2 HP Printer It is possible to connect HP printer to the NanoPhotometer via USB cable for direct printout. The following HP printers are tested and released: HP LaserJet 3030 HP LaserJet m1522nf MFP ... -
Page 16: Nano Photometer Specifications
NanoPhotometer NP80/N60/N50/C40 User Guide Version 1.3.2 Note: Plug in the LAN cable before starting the NanoPhotometer. Note: The maximal LAN cable length is 10 meters. Bit rate is 1 Gbit/s. HDMI There is an HDMI port located on the rear panel of the NanoPhotometer which is compatible with HDMI 1.4 (or better) cables to connect the NanoPhotometer to HDMI compatible monitors. -
Page 17: Etting Tarted
NanoPhotometer NP80/N60/N50/C40 User Guide Version 1.3.2 Software Compatibility Windows 7 (32 and 64 bit), Windows 8 (32 and 64 bit), OS X, iOS and Android Min. Requirement 4 inch screen; Smartphone/Tablet Apple: iPad 2, iPhone 5 and iOS 6 Android version 4.4, Quadcore 1.2 GHz with 1 GB RAM... - Page 18 NanoPhotometer NP80/N60/N50/C40 User Guide Version 1.3.2 Do not put the instrument into fire, as it may swell or explode (battery). Do not store at or use near any type of heat source, especially temperatures above 60°C or in an explosive atmosphere.
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Page 19: Software Installation
NPOS can store data either to a common directory or be configured to save to independent directories according to file format and/or instrument. NPOS can save data in an Implen .IDS format, PDF or as an Excel format file. Note: Please do not connect the instrument to a computer until the NanoPhotometer NPOS software is installed on the computer. -
Page 20: Installing Software On Computer
2. Connect the instrument to the computer using the USB cable provided Note: The Windows and Mac installation file is provided on the Implen USB flash drive which is included in the NanoPhotometer delivery and are available for free download in the download section of the Implen webpage. -
Page 21: First Steps And Configuration Wizard
IRST STEPS AND CONFIGURATION WIZARD Starting the Implen NPOS the first time an Implen configuration wizard is shown. Please confirm the End User License Agreement (EULA) and select the country in which the NanoPhotometer is used and confirm. -
Page 22: Ano Hotometer Asics
HOTOMETER ASICS The NanoPhotometer product line offers a complete solution for Nanovolume (NP80/N60/N50/C40) and standard cuvette (C40/NP80) applications. With the NanoPhotometer nanovolume applications the sample volume ranges from a minimum of 0.3 μl to a maximum sample volume of 2.0 µl (5 µl for using the submicroliter cell/C40). - Page 23 NanoPhotometer NP80/N60/N50/C40 User Guide Version 1.3.2 Stored Results Archive of stored results Stored Methods Collection of stored custom methods More Apps Additional applications found on a secondary method screen Define one or multiple wavelength between 200 – 900 nm (N50: 200 – 650 nm) for absorbance...
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Page 24: Icons
NanoPhotometer NP80/N60/N50/C40 User Guide Version 1.3.2 CONS Icon Name Action WiFi Hotspot WiFi hotsopt enabeld; can be disabled in preferences Assistance Opens the assistance page Battery Status Shows the actual battery status (only shown with optional battery pack) Preferences Opens the preferences page Home Screen Returns to the home screen with application icons for method selection. -
Page 25: Buttons
The Change to Cuvette button (NP80) is located at the bottom of the parameter window. The change to cuvette/nanovolume button toggles between nanovolume and cuvette applications. -
Page 26: Side Tab Bar
NanoPhotometer NP80/N60/N50/C40 User Guide Version 1.3.2 AB BAR On the left side of the measurement screen there is a vertical tab bar that contains four tabs including: parameters, data, graph, and table. The different tabs allow organizing the measurement screen. It is possible to show or hide the different areas on the screen. Default screen for computer show all areas, for the built-in screen and tablet version the table is hidden. - Page 27 NanoPhotometer NP80/N60/N50/C40 User Guide Version 1.3.2 Table area The table area collects defined results for all samples completed during the active method. The first column of the table indicates whether the measurement is saved ( ) or not saved (blank field).
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Page 28: Data Processing Dialogs
NanoPhotometer NP80/N60/N50/C40 User Guide Version 1.3.2 ATA PROCESSING DIALOGS Print Selecting the print data icon ( ) opens a full screen overlay dialog window with various save options. Note: The print icon is only shown, when a Dymo LabelWriter/HP printer is connected to the NanoPhotometer. - Page 29 In the rarely case that the micro SD card crashes data loss cannot be ruled out. Implen Document Source File Implen has a custom file format referred to as being an Implen Document Source (IDS) file. This is a hardcopy file to which no changes can be made. This file type contains all information including raw data, results, values, and parameters.
- Page 30 NanoPhotometer NP80/N60/N50/C40 User Guide Version 1.3.2 Excel File Data from the measurements can also be saved in an Excel file format. This file format is compatible with Microsoft Excel software versions 2007/2010/2013 and can be edited. This file type contains all information including raw data, results, and parameters. The saved file contains only the selected information at the time the file was saved.
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Page 31: Basic Operation
1. Select a method depending on your sample and set the parameter for the measurement. 2. Ensure that the sample window and the mirror in the sample arm are clean. 3. Use the integrated vortexer (N60 and NP80 only) to mix your sample to achieve an homogenous sample 4. -
Page 32: Cuvette Measurement Basics (C40/Np80)
The NanoPhotometer (NP80 and C40 only) is compatible with standard cuvettes having an 8.5 mm center height. The light path is indicated with a red status LED arrow for the NP80 model and with two white arrows for the C40 model. -
Page 33: Submicroliter Cell Measurements Basics (C40)
The Implen submicroliter cell can be used with the NanoPhotometer C40. Insert the submicroliter cell to the cell holder with the cell windows facing the light beam. We recommend facing the Implen logo to the right side. The light beam is directed from BACK to FRONT as indicated with the white arrows. - Page 34 Note: If there is no “Change to NanoVolume” button available in the method go to preferences/general and enable the NanoVolume option. We guarantee our submicroliter cell specifications only for Implen submicroliter cells which are aligned to the NanoPhotometer C40 by Implen.
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Page 35: Sample Handling Tips
The minimum volume that can be used for nanovolume samples is 0.3 µl (starting for NP80/N60/N50 dsDNA > 420 ng/µl and BSA > 12.6 mg/ml and for submicroliter cell in the NP40 dsDNA > 25 ng/µl and BSA > 0.7 mg/ml). For automatic path length setting at least 1µl is needed (NP80/N60/N50 only). - Page 36 NP80/N60/N50/C40 User Guide Version 1.3.2 The cuvette holder (NP80/C40 only) is compatible with standard 10 mm path length quartz, glass and plastic cuvettes with an optical height of 8.5 mm. It is also possible to use cuvettes with 5 mm, 2 mm or 1 mm path lengths, but there may be an adapter necessary.
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Page 37: Battery Operation
ATTERY PERATION The optional battery operation is only available for NP80-Touch, N60-Touch and C40-Touch models. A full battery pack can be operated for approximately 8 hours within normal usage, i.e. 20-30 measurements per hour. When the battery gets low there will be a first warning message “Battery low”... -
Page 38: Nano Photometer Applications
ROTOCOL 1. Select the Nucleic Acids icon on the home screen. To change between NanoVolume and cuvette application (NP80/C40 only) use the Change to Cuvette/NanoVolume button below the parameter area. 2. Select the nucleic acid type in the drop-down of the parameter area. - Page 39 When the cuvette holder has 37°C the color changes to green. Note: Only available for cuvette applications (NP80 and C40). 5. The background correction is enabled as the default. It is possible to disable the background correction with the toggle switch.
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Page 40: Calculations
NanoPhotometer NP80/N60/N50/C40 User Guide Version 1.3.2 ALCULATIONS Nucleic Acid Concentration To determine the concentration of nucleic acids in solution, the absorbance as measured at the wavelength of 260 nm is used. The function describing the relationship between concentration and absorbance is a modification of the Beer-Lambert law equation. The concentration of the nucleic acid samples can be calculated with or without background correction depending on if the background correction is turned on for the measurements. - Page 41 NanoPhotometer NP80/N60/N50/C40 User Guide Version 1.3.2 Table 2). The concentration of a dye-labeled nucleic acid is calculated with or without background/dye correction as follows: With background and with dye correction: C = [ ( A − A ) − (cf * (A −...
- Page 42 NanoPhotometer NP80/N60/N50/C40 User Guide Version 1.3.2 Table 2). The dye concentration is calculated with or without background correction as follows: With background correction: − A ) * Ð max,dye ε * 10 Without background correction: Đ max,dye ε * 10 Concentration in ng/µl...
- Page 43 NanoPhotometer NP80/N60/N50/C40 User Guide Version 1.3.2 Frequency of Incorporation (FOI) FOI is the degree of labeling based on dye incorporation in a labeled nucleic acid sample. It is generally expressed as the number of dye molecules incorporated per 1000 nucleotides. FOI...
- Page 44 NanoPhotometer NP80/N60/N50/C40 User Guide Version 1.3.2 Table 2. Dye Types, Absorbance Max, Extinction coefficient, and dye-dependent correction factors NanoPhotometer Dye Type Absorbance Dye-dependent Dye-dependent Model maximum of ext. coeff. correction factor ε Dye (nm) in M at 260 nm cf...
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Page 45: Protein Uv
NanoPhotometer NP80/N60/N50/C40 User Guide Version 1.3.2 this is generally indicative of contamination with guanidinium thiocyanate, a reagent commonly used in RNA purification and which absorbs over the 230 - 260 nm range. The ratios are calculated with or without background correction according to if the background... -
Page 46: Measurement Protocol
ROTOCOL 1. Select the Protein UV icon on the home screen. To change between NanoVolume and cuvette application (NP80/C40 only) use the Change to Cuvette/NanoVolume button below the parameter area. 2. Select the protein type in the drop-down of the parameter area: Options are: BSA, SA Mouse, SA Human, IgG Mouse, IgG Human, IgE Human, Lysozyme, OD1, Mol. -
Page 47: Calculations
When the cuvette holder has 37°C the color changes to green. Note: Only available for cuvette applications (NP80 and C40). 6. If a dye labeled sample is used please add a dye by tapping on the Add Dye Label option and select a dye from the drop-down list. - Page 48 NanoPhotometer NP80/N60/N50/C40 User Guide Version 1.3.2 With background correction: C = ( A ) * ε − A * Ð prot Without background correction: C = A * ε * Ð prot Concentration (mg/ml) Absorbance at 280 nm (10 mm path) Absorbance at 320 nm (10 mm path) ε...
- Page 49 NanoPhotometer NP80/N60/N50/C40 User Guide Version 1.3.2 Without background and without dye correction: C = A * ε * Ð prot Concentration in mg/ml Absorbance at 280 nm (10 mm path) Absorbance at 320 nm (10 mm path) Absorbance value at the absorbance maximum of the dye (10 mm path) max,dye ε...
- Page 50 Dye-dependent Dye-dependent Models maximum ext. coeff. correction ε of Dye (nm) in M factor at 280 nm NP80/N60/N50/C40 Alexa Fluor 350 19,000 0.19 NP80/N60/N50/C40 Alexa Fluor 405 34,500 0.70 NP80/N60/N50/C40 Alexa Fluor 488 71,000 0.11 NP80/N60/N50/C40 Alexa Fluor 532 81,000 0.09...
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Page 51: Protein Assays
Alexa Fluor 790 260,000 0.08 NP80/N60/N50/C40 Cy3 150,000 0.05 NP80/N60/C40 250,000 0.05 NP80/N60/C40 DyLight 649 250,000 0.04 NP80/N60/N50/C40 DyLight 488 70,000 0.15 NP80/N60/N50/C40 FITC 68,000 0.30 NP80/N60/N50/C40 Pacific Blue 30,000 0.20 NP80/N60/N50/C40 Pacific Orange 24,500 0.60 NP80/N60/N50/C40 pHrodo Green 75,000 0.20... -
Page 52: Measurement Protocol
ROTOCOL Note: It is not recommended to use the NanoVolume application for Protein Assays. For best results use the cuvette mode (NP80 and C40 models only) for the Protein Assays. 1. Select protein assays icon from home screen. To change between NanoVolume and cuvette application, use the Change to Cuvette/NanoVolume button below the parameter area (NP80/C40 only). - Page 53 When the cuvette holder has 37°C the color changes to green. Note: Only available for cuvette applications (NP80 and C40). 5. The baseline correction is disabled as the default. It is possible to choose between different wavelengths for the baseline correction.
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Page 54: Calculations
NanoPhotometer NP80/N60/N50/C40 User Guide Version 1.3.2 8. Once Standard curve is created or loaded it will be used for concentration calculations in the method. It might be necessary to do a blank measurement. 9. Apply sample and press the sample button to initiate the measurement. -
Page 55: Measurement Protocol
NanoPhotometer NP80/N60/N50/C40 User Guide Version 1.3.2 EASUREMENT ROTOCOL Note: If a kinetic is started from a control device via WiFi connection (tablet or smartphone) set the auto lock of the tablet or smartphone to never. Otherwise the kinetics will be interrupted when the smartphone or tablet is locked, because of losing the WiFi connection. -
Page 56: Calculations
NanoPhotometer NP80/N60/N50/C40 User Guide Version 1.3.2 Note: During the kinetics is running it is not possible to change the parameters or save or delete data. Change of parameters is only possible before starting the kinetic. Save and delete data is only available after the kinetic is stopped. -
Page 57: Measurement Protocol
ROTOCOL Note: It is not recommended to use the NanoVolume application for the OD600 application. For best results use the cuvette mode (NP80 and C40 models only) for OD600 measurements. 1. Select the OD600 icon on the home screen 2. For Cuvette Application: Select the path length depending on the used cuvette. -
Page 58: Calculations
NanoPhotometer NP80/N60/N50/C40 User Guide Version 1.3.2 3. If it is desired to heat the cuvette compartment to 37°C turn the toggle switch on for heated cuvette. Once the cuvette reaches the desired temperature the switch will turn from orange to green. -
Page 59: More Apps : Wavelength
When the cuvette holder has 37°C the color changes to green. Note: Only available for cuvette applications (NP80 and C40). 4. Enter desired wavelength () to be measured. It is possible to measure up to 20 wavelengths simultaneously. More wavelength () options are added by selecting the wavelength button. -
Page 60: Calculations
NanoPhotometer NP80/N60/N50/C40 User Guide Version 1.3.2 6. Option to smooth the graph with different boxcars. off, 1 = boxcar 11 (default), 2 = boxcar 21 and 3 = boxcar 61 7. Option to set/calculate a dilution factor for manual diluted samples. -
Page 61: More Apps : Wavescan
ETHOD VERVIEW Using the wavescan application it is possible to obtain the complete spectral scan for a defined wavelength range between 200 - 900 nm (NP80/N60/C40) or from 200 - 650 nm (N50). EASUREMENT ROTOCOL 1. Select the More Apps icon... -
Page 62: Calculations
NanoPhotometer NP80/N60/N50/C40 User Guide Version 1.3.2 6. Option to smooth the graph with different boxcars. off, 1 = boxcar 11 (default), 2 = boxcar 21 and 3 = boxcar 61 7. Option to set/calculate a dilution factor for manual diluted samples. - Page 63 When the cuvette holder has 37°C the color changes to green. Note: Only available for cuvette applications (NP80 and C40). 4. Enter desired wavelengths ( 1-1 and 1-2) for ratio calculation. It is possible to measure up to 20 absorbance/ratios simultaneously. More wavelength for ratio calculation can be added by selecting the Add Ratio button.
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Page 64: Calculations
NanoPhotometer NP80/N60/N50/C40 User Guide Version 1.3.2 9. Use a lint-free laboratory wipe to clean both the sample window and mirror prior to applying the next sample. Note: It could be helpful to apply the blank a second time and read it as a sample to ensure a proper blank. - Page 65 3. If it is desired to heat the sample to 37°C use the toggle switch to turn on cell holder heater. When the cuvette holder has 37°C the color changes to green. Note: Only available for cuvette applications (NP80 and C40). 4. Default wavelength is 260 nm but can be changed, depending on the application.
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Page 66: Calculations
NanoPhotometer NP80/N60/N50/C40 User Guide Version 1.3.2 initiate the measurement. ALCULATIONS In this method, the concentration of the sample is calculated based on the Beer-Lambert law given the user specified wavelength of interest and user defined extinction coefficient. The equations for calculating concentration with and without background correction are as follows: Without background correction: ��... - Page 67 When the cuvette holder has 37°C the color changes to green. Note: Only available for cuvette applications (NP80 and C40). 4. The baseline correction is disabled as the default. It is possible to choose between different wavelengths for the baseline correction.
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Page 68: Calculations
USTOM There is an option for designing customer specific Custom Apps which can be loaded to the NanoPhotometer. For more information about designing custom applications to suit individual research needs please contact Implen directly for assistance. TORED ESULTS The Stored Results icon opens the directories of folders containing files of results that have been previously saved. -
Page 69: Stored Methods
NanoPhotometer NP80/N60/N50/C40 User Guide Version 1.3.2 TORED ETHODS The stored methods icon opens the directories of folders containing methods stored by the user. On the left side of the screen all available directories are shown: NanoPhotometer and/or USB flash drive. To open a folder click on the icon, for selecting the folder click on the folder name. -
Page 70: Display
Toggle Switch to en-/disable the WiFi access point. LLUMINATION AMPLE INDOW Toggle switch to switch on/off the illumination of the sample window (for NP80/N60/N50 only) Note: Only available for NP80/N60/N50 models. NABLE OLUME Toggle switch to enable the submicroliter cell option in the NanoPhotometer C40 only There is a list of available dye-labels for both nucleic acid dye and protein dye methods. -
Page 71: Troubleshooting
The error message is also shown on top of all other screen and all performed measurements including the saved files of those measurements. If the self-calibration test fails please contact the Implen Support Team. SSISTANCE The Assistance menu includes: support, report a problem (only available for tablet and computer versions), software maintenance and legal as functions to help with any technical issues or questions that may arise with the NanoPhotometer. -
Page 72: Report Problem
Once the form is completed and the send button is selected a message will be sent directly to Implen and the appropriate support person will contact as soon as possible to provide further support. -
Page 73: Contact Implen
Note: Please update also in a second step the client software (PC/MAC, tablet, smartphone). ONTACT MPLEN There is an option to contact Implen for instruments connected to the internet. For any support issues and questions please contact the Implen team directly: Europe, Asia, South Pacific, Middle East and Africa... -
Page 74: Maintenance
(during production and quality control), certified engineers and calibrated test equipment, approved to ISO 9001 standard, automatic self-diagnostic calibration test during start of the NanoPhotometer, result is documented in each data output file, and possibility to save a Implen Document Source (IDS) file (no data manipulation possible). -
Page 75: Cleaning And General Care
The warranty included in the conditions of supply is valid for 12 months and is valid only if the product has been used according to the instructions supplied. Implen or your supplier can accept no liability of loss or damage arising from the faulty or incorrect use of this product... -
Page 76: Alphabetical Appendix
NanoPhotometer NP80/N60/N50/C40 User Guide Version 1.3.2 LPHABETICAL PPENDIX Absorbance Ratio ............. 62 Graph area ............... 27 Calculations ............64 Graph Overlay ............27 Measurement Protocol ......... 62 Accessories ............11–13 Assistance ..............71 Maintenance ............72 HDMI ................ 16 Report Problem ............ 72 HP Printer .............. - Page 77 Reset ................ 72 NanoPhotometer C40 ........... 10 Results area ............. 26 NanoPhotometer N50 ..........9 NanoPhotometer N60 ..........8 NanoPhotometer NP80 .......... 7 NanoVolume ............31, 35 Safety Information ............ 17 NPOS ............... 19 Save Data ............28, 29 Nucleic Acids............38 Software Calculations ............