Implen NanoPhotometer NP80 User Manual
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Implen NanoPhotometer NP80 User Manual

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NanoPhotometer
NP80/N60/N50/C40
User Manual
Version 3.1.1
Software Version 3.1.13220

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Summary of Contents for Implen NanoPhotometer NP80

  • Page 1 ® NanoPhotometer NP80/N60/N50/C40 User Manual Version 3.1.1 Software Version 3.1.13220...
  • Page 2 NP80/N60/N50/C40 User Guide Version 3.1.1 ® Declaration of conformity for the NanoPhotometer (C40/N50/N60/NP80) ® This is to certify that the Implen NanoPhotometer conforms to the requirements of the following directives: 2014/35/EU Low Voltage Equipment Safety Directive 2014/30/EU Electromagnetic compatibility (EMC) directive...

  • Page 3: Table Of Contents

    ® NanoPhotometer NP80/N60/N50/C40 User Guide Version 3.1.1 Contents ® ............... 8 HOTOMETER AT A LANCE ® ....................8 HOTOMETER VERVIEW ® NP80 ........................ 9 HOTOMETER ® N60 ......................... 10 HOTOMETER ® N50 ......................... 11 HOTOMETER ® C40 ......................... 12 HOTOMETER (NP80/N60/N50/C40) ................
  • Page 4 ® NanoPhotometer NP80/N60/N50/C40 User Guide Version 3.1.1 ......................43 ATTERY PERATION ® ..............44 HOTOMETER PPLICATIONS ........................44 UCLEIC CIDS .......................... 44 ETHOD VERVIEW ....................... 44 EASUREMENT ROTOCOL ..........................48 ALCULATIONS ........................54 ROTEIN .......................... 54 ETHOD VERVIEW ....................... 56 EASUREMENT ROTOCOL ..........................
  • Page 5 ® NanoPhotometer NP80/N60/N50/C40 User Guide Version 3.1.1 ..................89 TANDARD URVE .......................... 89 ETHOD VERVIEW ......................89 EASUREMENTS ROTOCOL ..........................92 ALCULATIONS ......................... 93 USTOM ......................93 TORED ESULTS ......................94 TORED ETHODS ....................95 REFERENCES ..........................95 ENERAL ..........................95 ATE AND ............................
  • Page 6 ® NanoPhotometer NP80/N60/N50/C40 User Guide Version 3.1.1 ....................... 108 NSTALL RINTER RIVER ............................108 EGAL ........................108 RADEMARKS ........................ 109 ONTACT MPLEN ....................110 AINTENANCE ..................110 AINTENANCE ECHNOLOGY ......................110 EPLACEMENT ARTS ................... 111 LEANING AND ENERAL ....................... 111 ARRANTY 10.
  • Page 7 ® NanoPhotometer NP80/N60/N50/C40 User Guide Version 3.1.1 ..................... 127 ESEMBALAJE E INSTALACIÓN 8 – S 17. A ..........128 PPENDIX PEKTROFOTOMETERINSTALLATION ....................128 ÄKERHETSINFORMATION ....................129 PPACKNING OCH PLACERING 9 – I 18. A ........130 PPENDIX NSTALAÇÃO DE SPECTROFOTÓMETRO ....................

  • Page 8: Nano Photometer ® Overview

    HOTOMETER VERVIEW ® The Implen NanoPhotometer spectrophotometer is a mobile, simple to use UV/Visible instrument with a CCD array detector with options ranging from nanovolume to standard cuvette and all-in-one solutions. The N50, N60, and NP80 spectrophotometers measure sample volumes ranging from 0.3 - 2 µl.

  • Page 9: Nano Photometer ® Np80

    ® NanoPhotometer NP80/N60/N50/C40 User Guide Version 3.1.1 ® NP80 HOTOMETER Item Number Touchscreen Battery Pack NP80 NP80-Touch NP80-Mobile...

  • Page 10: Nano Photometer ® N60

    ® NanoPhotometer NP80/N60/N50/C40 User Guide Version 3.1.1 ® HOTOMETER Item Number Touchscreen Battery Pack N60-Touch N60-Mobile...

  • Page 11: Nano Photometer ® N50

    ® NanoPhotometer NP80/N60/N50/C40 User Guide Version 3.1.1 ® HOTOMETER Item Number Touchscreen Battery Pack N50-Touch...

  • Page 12: Nano Photometer ® C40

    ® NanoPhotometer NP80/N60/N50/C40 User Guide Version 3.1.1 ® HOTOMETER Item Number Touchscreen Battery Pack C40-Touch C40-Mobile...

  • Page 13: Instrument Rear Panel (Np80/N60/N50/C40)

    ® NanoPhotometer NP80/N60/N50/C40 User Guide Version 3.1.1 (NP80/N60/N50/C40) NSTRUMENT ANEL ® To boot/shut down the NanoPhotometer click shortly (< 1 second) on the power on/off button. Note: Long clicks (> 3 seconds) initiate a hard reset. Only activate a hard reset of the unit when necessary.

  • Page 14: Accessories

    ® NanoPhotometer NP80/N60/N50/C40 User Guide Version 3.1.1 CCESSORIES TANDARD CCESSORIES  Connecting Cable ® USB connecting cable to connect the NanoPhotometer to a computer to control the ® NanoPhotometer via computer (please see page 23 Software Installation). ®  NanoPhotometer Power Adapter ®...
  • Page 15 ® NanoPhotometer NP80/N60/N50/C40 User Guide Version 3.1.1  Standard Solution (NP80, N60, N50) ® The NanoPhotometer is recalibration free therefore it is not necessary to review the photometric accuracy on a regular basis. If the SOPs of a laboratory require a routine control of the photometric accuracy standard solutions can be used.

  • Page 16: Nstallation

    NanoPhotometer NP80/N60/N50/C40 User Guide Version 3.1.1  Field Kit Implen aluminum roller case for safe transportation, designed to fit in most airplane overhead bins; special compartments for all necessary accessories, cleaning tools and samples. ® Note: Delivered w/o NanoPhotometer and accessories ®...
  • Page 17 ® NanoPhotometer NP80/N60/N50/C40 User Guide Version 3.1.1 Printout for DYMO LabelWriter 4XL Printout for DYMO LabelWriter 450 Note: The printouts are optimized for the label size of the DYMO LabelWriter 4XL. All other DYMO printers can be used. However, the font size will be zoomed to utilized paper size.

  • Page 18: Connectivity

    ® NanoPhotometer NP80/N60/N50/C40 User Guide Version 3.1.1  Printer ® Printing from the NanoPhotometer is possible via USB and through network connection. ® Network printing should be possible with AirPrint / IPP compatible printers supporting PDF format. Note: IPP version 2.2 is required and some printer configuration settings might need to be changed in ®...

  • Page 19: Nano Photometer ® Specifications

    ® NanoPhotometer NP80/N60/N50/C40 User Guide Version 3.1.1  WiFi ® The NanoPhotometer is equipped with WiFi, which can be used as a WiFi network or as a WiFi ® Hotspot. The WiFi network allows data transfer and direct printing via AirPrint / IPP compatible printers supporting PDF format.
  • Page 20 ® NanoPhotometer NP80/N60/N50/C40 User Guide Version 3.1.1 Optical Specifications 200 – 900 nm (N50: 200 - 650 nm) Wavelength Scan Range 3.5 – 6.0 seconds Time for Full Scan Range Wavelength Reproducibility ± 0.2 nm (N50: ± 1nm) Wavelength Accuracy ±...

  • Page 21: Getting Started

    ® NanoPhotometer NP80/N60/N50/C40 User Guide Version 3.1.1 ETTING TARTED PECTROPHOTOMETER NSTALLATION Note: Translations of the Safety Information and Unpacking and Positioning are available in Appendix 1 (Czech), Appendix 2 (Dutch), Appendix 3 (French), Appendix 4 (German), Appendix 5 (Italian), Appendix 6 (Polish), Appendix 7 (Spanish), Appendix 8 (Swedish) and Appendix 9 (Portuguese) AFETY NFORMATION Before commencing installation, please take time to familiarize yourself with warning labels and...

  • Page 22: Unpacking And Positioning

    Always carry the instrument by holding the main corpus of the instrument and not e.g. on the optional attached display or NanoVolume pedestal. The equipment must be connected to power with the 60W power supply/cord supplied by Implen (90W with battery pack). The power outlet must have a protective conductor (earth/ground). It can be used on 90-250 V, 50-60 Hz power supply system.

  • Page 23: Software Installation

    NPOS can store data either to a common directory or be configured to save to independent directories according to file format and/or instrument. NPOS can save data in an Implen .IDS format, PDF or as an Excel format file. ®...

  • Page 24: Installing Software On Computer

    Note: If a previous version of the NPOS software is already installed on the PC, remove the USB cable and uninstall the NPOS software before installing the new software version. Note: The Windows and Mac installation file is provided on the Implen USB flash drive which is ®...

  • Page 25: Installing Nano Photometer ® App On Tablet Or Smartphone

    ® NanoPhotometer NP80/N60/N50/C40 User Guide Version 3.1.1 The installer should be logged in with full administration rights. If you have insufficient privileges, installation may fail. If in doubt consult your PC administrator. 3. Open the NPOS software and choose the preferred connection. For USB, connect the ®...

  • Page 26: First Steps And Configuration Wizard

    IRST STEPS AND CONFIGURATION WIZARD When starting the Implen NPOS the first time an Implen configuration wizard is shown. Please accept ® the End User License Agreement (EULA) and select the country in which the NanoPhotometer used and confirm.

  • Page 27: Nano Photometer ® Basics

    ® NanoPhotometer NP80/N60/N50/C40 User Guide Version 3.1.1 ® HOTOMETER ASICS ® The NanoPhotometer product line offers a complete solution for Nanovolume (NP80/N60/N50/C40) ® and standard cuvette (C40/NP80) applications. With the NanoPhotometer NanoVolume applications, sample volume ranges from a minimum of 0.3 μl to a maximum sample volume of 2.0 µl (5 µl for using the submicroliter cell/C40).
  • Page 28 ® NanoPhotometer NP80/N60/N50/C40 User Guide Version 3.1.1 BCA (562 nm), Bradford (595 nm), Lowry (750 nm), and Biuret Protein Assays Assays (546 nm) Measures cell density at 600 nm (or in a range of 200 – 900 OD600 Stored Results Archive of stored results Stored Methods Collection of stored custom methods...

  • Page 29: Icons

    ® NanoPhotometer NP80/N60/N50/C40 User Guide Version 3.1.1 CONS Icon Name Action WiFi Network WiFi network active; status of WiFi connection WiFi Hotspot WiFi hotspot active Assistance Opens the assistance page Battery Status Shows the actual battery status (only shown with optional battery pack) Preferences Opens the preferences page Home Screen...

  • Page 30: Buttons

    ® NanoPhotometer NP80/N60/N50/C40 User Guide Version 3.1.1 UTTONS Prior to any sample measurement at the start of a new method a blank measurement of either water or the buffer of the samples is required to give ® the NanoPhotometer a reference of what zero should be. It is recommended to re-apply the blank and measure it as a sample to ensure the graph of the blank spectra is a flat line.

  • Page 31: Side Tab Bar

    ® NanoPhotometer NP80/N60/N50/C40 User Guide Version 3.1.1 AB BAR On the left side of the measurement screen there is a vertical tab bar that contains four tabs including: parameters, data, graph, and table. The different tabs allow the user to organize the measurement screen.
  • Page 32 ® NanoPhotometer NP80/N60/N50/C40 User Guide Version 3.1.1 selection is 30). The second column of the table indicates whether the measurement is saved ( ) or not saved (blank field). With the edit button the sample name of a single sample can be edited. 1.

  • Page 33: Data Processing Dialogs

    ® NanoPhotometer NP80/N60/N50/C40 User Guide Version 3.1.1 ATA PROCESSING DIALOGS  Print Selecting the print data icon ( ) opens a full screen overlay dialog window with various print options. The print icon is only shown if a printer is available. The print command will always print to a DYMO or HP printer, which is directly connected to the ®...
  • Page 34 Note: In the smartphone app there are always all measurements saved, no selection possible. Save as Type With the save type option it is possible to specify the file type for saving. File type options include Excel, PDF and Implen Document Source (IDS). It is possible to save different file formats simultaneously. ®...
  • Page 35 User Guide Version 3.1.1 Implen Document Source File Implen has a custom file format referred to as an Implen Document Source (IDS) file. This is a hardcopy file to which no changes can be made. This file type contains all information including raw data, results, values, and parameters.

  • Page 36: Basic Operation

    5 mm, 2 mm, 1 mm and 0.5 mm path length quartz, glass, or plastic cuvettes with a center height of 8.5 cm. ® Note: NanoVolume application in the NanoPhotometer C40 is only available with the optional accessory “submicroliter cell” from Implen. (N50/N60/NP80) OLUME EASUREMENT ASICS 1.

  • Page 37: Cuvette Measurement Basics (C40/Np80)

    Note: Do not use aggressive solvents such as strong acids or bases or organic solvents at any time (see also page 41 solvent compatibility). If unsure please contact support@implen.de for detailed information about your specific reagent/solvent. 7. It is possible to enter a sample name for each sample in the input window “enter sample name”.
  • Page 38 ® NanoPhotometer NP80/N60/N50/C40 User Guide Version 3.1.1 Note: For the NP80 the cuvette compartment needs to be activated by the “Change to Cuvette” button in the parameter area. Once the cuvette option is activated the sample compartment door will be opened automatically and a red arrow above the cuvette compartment will appear. The arrow indicates the light path.

  • Page 39: Submicroliter Cell Measurements Basics (C40)

    C40. Insert the submicroliter cell to the cell holder with the cell windows facing the light beam. We recommend facing the Implen logo to the right side. The light beam is directed from BACK to FRONT as indicated with the white arrows.

  • Page 40: Sample Handling Tips

    Note: Do not use aggressive solvents such as strong acids or bases or organic solvents at any time (see also page 41 solvent compatibility). If unsure please contact support@implen.de for detailed information about your specific reagent/solvent. 8. It is possible to enter a sample name for each sample in the input window “enter sample name”.
  • Page 41 ® NanoPhotometer NP80/N60/N50/C40 User Guide Version 3.1.1 use a slightly wet lint-free laboratory wipe (with water or 70% EtOH depending on sample type) to thoroughly clean the sample surface.  It is mandatory that the metal contact face around the measurement window and the mirror is clean.

  • Page 42: Data Transfer

    Note: Highly concentrated acids and bases are not recommended. It is recommended to wipe the sample surface with a lint-free laboratory wipe immediately upon completion of each measurement. For more information about compatibility of specific solvents not listed above, please contact the Implen support team (support@implen.de) to check the compatibility. RANSFER ®...

  • Page 43: Nanophotometer Np80/N60/N50/C40

    ® NanoPhotometer NP80/N60/N50/C40 User Guide Version 3.1.1 ATTERY PERATION ® The optional battery operation is only available for the NanoPhotometer NP80/N60/C40 models (NP80-Mobile, N60-Mobile and C40-Mobile). A full battery pack can be operated for approximately 10 hours within normal usage, i.e. 20-30 measurements per hour. When the battery gets low there will be a first warning message “Battery low”...

  • Page 44: Nano Photometer

    ® NanoPhotometer NP80/N60/N50/C40 User Guide Version 3.1.1 ® HOTOMETER PPLICATIONS ® The NanoPhotometer comes with pre-programmed applications as well as the ability to create custom applications. Any application method can be selected by tapping the icon once or clicking on the icon (computer based software). Note: Within each method selection of nanovolume or cuvette mode (NP80/C40) is possible.
  • Page 45 ® NanoPhotometer NP80/N60/N50/C40 User Guide Version 3.1.1 entered from the range of 15 - 150. 4. For NanoVolume application: Select the volume of sample to be applied. Note: 1 - 2 µl (default): automatic path length setting; 0.3 µl measures only the 0.07 mm path length (for samples with concentrations >...
  • Page 46 ® NanoPhotometer NP80/N60/N50/C40 User Guide Version 3.1.1 Note: A new Blank is recommended when changing the dilution lid. 5. If it is desired to heat the sample to 37°C use the toggle switch to turn on cell holder heater. When the cuvette holder has reached 37°C the toggle color changes to green.
  • Page 47 ® NanoPhotometer NP80/N60/N50/C40 User Guide Version 3.1.1 10. Option to set/calculate a dilution factor for manual diluted samples. 11. Apply the blank ddH 0 or buffer to the illuminated sample window for the reference measurement and select blank to initiate the reading. Note: The illumination of the sample window can be switched off in the preferences.

  • Page 48: Calculations

    ® NanoPhotometer NP80/N60/N50/C40 User Guide Version 3.1.1 initiate the measurement. ALCULATIONS  Nucleic Acid Concentration To determine the concentration of nucleic acids in solution, the absorbance as measured at the wavelength of 260 nm is used. The function describing the relationship between concentration and absorbance is a modification of the Beer-Lambert law equation.
  • Page 49 ® NanoPhotometer NP80/N60/N50/C40 User Guide Version 3.1.1 Table 2). The concentration of a dye-labeled nucleic acid is calculated with or without background/dye correction as follows: With background and with dye correction: C = [ ( A − A ) − (cf * (A −...
  • Page 50 ® NanoPhotometer NP80/N60/N50/C40 User Guide Version 3.1.1 Table 2). The dye concentration is calculated with or without background correction as follows: With background correction: − A ) * Ð max,dye ε * 10 Without background correction: Đ max,dye ε * 10 Concentration in ng/µl Absorbance value at the absorbance maximum of the dye (10 mm path) max,dye...
  • Page 51 ® NanoPhotometer NP80/N60/N50/C40 User Guide Version 3.1.1 Frequency of Incorporation (dye per 1,000 bases) Absorbance at 260 nm (10 mm path) Absorbance at selected background wavelength (10 mm path) Absorbance value at the absorbance maximum of the dye (10 mm path) max,dye ε...
  • Page 52 ® NanoPhotometer NP80/N60/N50/C40 User Guide Version 3.1.1 Table 2. Dye Types, Absorbance Max, Extinction coefficient, and dye-dependent correction factors ® NanoPhotometer Dye Type Absorbance Dye-dependent Dye-dependent Model maximum of ext. coeff. correction factor ε Dye (nm) in M at 260 nm cf NP80/N60/N50/C40 Alexa Fluor 350 18,400...
  • Page 53 ® NanoPhotometer NP80/N60/N50/C40 User Guide Version 3.1.1 this is generally indicative of contamination with guanidinium thiocyanate, a reagent commonly used in RNA purification and which absorbs over the 230-260 nm range. If a ratio is detected out of the acceptable range an alert icon is shown in the results/table area.

  • Page 54: Protein Uv

    ® NanoPhotometer NP80/N60/N50/C40 User Guide Version 3.1.1 ROTEIN ETHOD VERVIEW The Protein UV method exploits the inherent absorbance of proteins at 280 nm in combination with the Beer-Lambert Law, where each protein is characterized by a protein specific extinction coefficient (ε) which can be used to determine total protein concentration of a solution. The intrinsic absorbance of proteins is due to the presence of aromatic amino acids in their structure, primarily tryptophan and tyrosine, as well as cysteine (oxidized cysteine residues in a disulphide bond).
  • Page 55 ® NanoPhotometer NP80/N60/N50/C40 User Guide Version 3.1.1 Table 3). However, if the protein of interest is not included in the pre-programed methods it is also possible to manually enter the specific ε for the protein of interest using the Mol. Ext. Coefficient or Ext.

  • Page 56: Measurement Protocol

    ® NanoPhotometer NP80/N60/N50/C40 User Guide Version 3.1.1 Table 3). The corresponding extinction coefficient of the dye is used along with the Beer- Lambert Law to determine the dye concentration. Note: It is important to ensure the extinction coefficient and units entered are correct in order to ensure that calculations are performed properly for accurate concentration values.
  • Page 57 ® NanoPhotometer NP80/N60/N50/C40 User Guide Version 3.1.1 Select the volume of sample to be applied. Note: 1-2 µl (default): automatic path length change; 0.3 µl measures only the 0.07 mm path length (possible for samples with concentrations e.g. BSA > 12.6 mg/ml) For cuvette application: Select the path length depending on the used cuvette.
  • Page 58 ® NanoPhotometer NP80/N60/N50/C40 User Guide Version 3.1.1 6. Air bubble recognition is disabled as a default. When enabled it detects air bubbles, lint residues and poor conditions of the sample. Note: Lint residues and bad sample conditions are detected even if the air bubble recognition is set to off.

  • Page 59: Calculations

    ® NanoPhotometer NP80/N60/N50/C40 User Guide Version 3.1.1 10. Apply the blank ddH 0 or buffer to the illuminated sample window for the reference measurement and select blank to initiate the reading. Note: The illumination of the sample window can be switched off in the preferences.
  • Page 60 ® NanoPhotometer NP80/N60/N50/C40 User Guide Version 3.1.1 Concentration (mg/ml) Absorbance at 280 nm (10 mm path) Absorbance at selected background wavelength (10 mm path) ε Extinction coefficient/protein factor in g*cm/l prot Ð Dilution factor...
  • Page 61 ® NanoPhotometer NP80/N60/N50/C40 User Guide Version 3.1.1 Table 3. Protein extinction coefficients (ε prot ε Ext. Coeff. [l/g*cm] Mol. Ext. Coeff. [M MW [g/mol] [ g* cm/l] Type prot 0.667 44,289 66,400 1.50 0.670 44,220 66,000 SA Mouse 1.49 0.582 40,370 69,365 SA Human...
  • Page 62 ® NanoPhotometer NP80/N60/N50/C40 User Guide Version 3.1.1 maximum of the dye, and a dye-specific extinction coefficient (see Table 4). The dye concentration is calculated with or without background correction for are as follows: With background correction: − A ) * Ð) max,dye ε...
  • Page 63 ® NanoPhotometer NP80/N60/N50/C40 User Guide Version 3.1.1 Degree of labeling/dye per protein ratio Absorbance value at the absorbance maximum of the dye (10 mm path) max,dye Absorbance at 280 nm (10 mm path) Absorbance at selected background wavelength (10 mm path) ε...

  • Page 64: Protein Assays

    ® NanoPhotometer NP80/N60/N50/C40 User Guide Version 3.1.1 detected out of the acceptable range an alert icon is shown in the results/table area. A click on the alert icon shows additional information. The ranges for acceptable ratio values can be defined in preferences. The ratio is calculated with or without background correction according to if the background correction is activated during the measurements or not as follows: Without background correction:...

  • Page 65: Measurement Protocol

    ® NanoPhotometer NP80/N60/N50/C40 User Guide Version 3.1.1  Lowry Assay Method is based on the Biuret reaction. Under alkaline conditions the divalent copper ion forms a complex with peptide bonds in which it is reduced to a monovalent ion. Monovalent copper ion and the radical groups of tyrosine, tryptophan, and cysteine react with Folin reagent to produce an unstable product that becomes reduced to molybdenum/tungsten blue.
  • Page 66 ® NanoPhotometer NP80/N60/N50/C40 User Guide Version 3.1.1 For NanoVolume Application: Select the dilution depending on the sample concentration Note: There is no automatic path length setting available in this method. Select either a virtual dilution of 15 (path length 0.67 mm) or of 140 (path length 0.07 mm) depending on your sample concentration.
  • Page 67 ® NanoPhotometer NP80/N60/N50/C40 User Guide Version 3.1.1 7. Select curve fit type: Options are linear regression, zero regression (forces the straight line through the origin) and 2 order regression 8. Select Unit 9. Add up to 20 Concentrations by clicking on the Add Concentration button. Added concentrations can be deleted with Enter the concentrations of the standard curve.
  • Page 68 ® NanoPhotometer NP80/N60/N50/C40 User Guide Version 3.1.1 10. Select Replicates none, 2 or 3 11. Measure a blank and depending on the replicate selection, all required concentrations. Absorbances of the replicates will be shown in the results area and if replicates are selected a mean value for each standard. It is possible to exclude single measurements from the curve calculation by switching the toggle switch off.

  • Page 69: Calculations

    ® NanoPhotometer NP80/N60/N50/C40 User Guide Version 3.1.1 ALCULATIONS Protein concentration is determined using the standard curve by correlating absorbance values of samples with known concentration to calculate the concentration of the unknown sample. In order to maintain accuracy and precision please ensure that the R value of the standard curve is 0.95 or greater.
  • Page 70 ® NanoPhotometer NP80/N60/N50/C40 User Guide Version 3.1.1 For NanoVolume Application: Select the dilution depending on the sample concentration. Note: There is no automatic path length setting available in this method. Select either a virtual dilution of 15 (path length 0.67 mm) or of 140 (path length 0.07 mm). For NanoVolume application with submicroliter cell (C40 only): Select Lid depending on your sample concentration (for concentration ranges of different lids see page 39 Submicroliter Cell Measurements Basics).

  • Page 71: Calculations

    ® NanoPhotometer NP80/N60/N50/C40 User Guide Version 3.1.1 3,600 seconds (N50: 10–3,600 sec.), depending on the duration time.  Enter the delay time in seconds before the first measurement is taken. Possible delay time is between 0–3,600 seconds, depending on the duration time. Note: A maximum of 500 samples is possible.
  • Page 72 ® NanoPhotometer NP80/N60/N50/C40 User Guide Version 3.1.1 Growth of bacterial cells typically progresses through a series of consecutive phases including: lag, log, stationary and decline (see Figure 1). In general, cells should be harvested towards the end of the log phase using the optical density of the samples to determine when this point has been reached.

  • Page 73: Measurement Protocol

    ® NanoPhotometer NP80/N60/N50/C40 User Guide Version 3.1.1 Figure 1 Bacterial growth curve EASUREMENT ROTOCOL 1. Select the OD600 icon on the home screen To change between NanoVolume and cuvette application, use the Change to Cuvette/NanoVolume button below the parameter area (NP80/C40 only).
  • Page 74 ® NanoPhotometer NP80/N60/N50/C40 User Guide Version 3.1.1 For NanoVolume application with submicroliter cell (C40 only): Select Lid depending on your sample concentration (for concentration ranges of different lids see page 39 Submicroliter Cell Measurements Basics). Required sample volume for each lid is shown. Note: A new Blank is recommended when changing the dilution lid.

  • Page 75: Calculations

    ® NanoPhotometer NP80/N60/N50/C40 User Guide Version 3.1.1 8. Option to smooth the graph with different boxcars. Options: Off, 1 = boxcar 11 (default), 2 = boxcar 21 and 3 = boxcar 61 9. Option to set/calculate a dilution factor for manual diluted samples. 10.

  • Page 76: More Apps

    ® NanoPhotometer NP80/N60/N50/C40 User Guide Version 3.1.1 Correction factor for spectrophotometer multiplier Multiplier of sample The More Apps icon located on the home screen opens another menu screen with access to ® icons for additional applications available on the NanoPhotometer .
  • Page 77 ® NanoPhotometer NP80/N60/N50/C40 User Guide Version 3.1.1 For NanoVolume application with submicroliter cell (C40 only): Select Lid depending on your sample concentration (for concentration ranges of different lids see page 39 Submicroliter Cell Measurements Basics). Required sample volume for each lid is shown. Note: A new Blank is recommended when changing the dilution lid.
  • Page 78 ® NanoPhotometer NP80/N60/N50/C40 User Guide Version 3.1.1 7. Option to smooth the graph with different boxcars. Options: Off, 1 = boxcar 11 (default), 2 = boxcar 21 and 3 = boxcar 61 8. Option to set/calculate a dilution factor for manual diluted samples. 9.

  • Page 79: Calculations

    ® NanoPhotometer NP80/N60/N50/C40 User Guide Version 3.1.1 11. Apply sample to the sample window and press the sample button to initiate the measurement. ALCULATIONS  Absorbance Calculation: Absorbance is formally defined as the decimal logarithm (base 10) of the reciprocal of transmittance: A = log ( ) = - log T...
  • Page 80 ® NanoPhotometer NP80/N60/N50/C40 User Guide Version 3.1.1 (NP80/C40 only). 3. For NanoVolume application: Select the dilution depending on the sample concentration Note: There is no automatic path length setting in this method. Select either a virtual dilution of 15 (path length 0.67 mm) or of 140 (path length 0.07 mm) For cuvette application: Select the path length depending on the used cuvette.
  • Page 81 ® NanoPhotometer NP80/N60/N50/C40 User Guide Version 3.1.1 Note: If samples with a different wavelength range are selected, the graphs are shown on full scan range of 200–900 nm (N50: 200-650 nm). 6. Baseline correction is set to off as default. Enabling the baseline corrections shows a list with different wavelength options: 377 nm, 604 nm, 650 nm, 770 nm (N/A N50) and 823 nm (N/A N50).

  • Page 82: Calculations

    ® NanoPhotometer NP80/N60/N50/C40 User Guide Version 3.1.1 10. Use a lint-free laboratory wipe to clean both the sample window and mirror prior to applying the next sample. Note: It can be helpful to apply the blank a second time and read it as a sample to ensure a proper blank.
  • Page 83 ® NanoPhotometer NP80/N60/N50/C40 User Guide Version 3.1.1 3. For NanoVolume application: Select the dilution depending on the sample concentration Note: There is no automatic path length setting in this method. Select either a virtual dilution of 15 (path length 0.67 mm) or of 140 (path length 0.07 mm). For cuvette application: Select the path length depending on the used cuvette.
  • Page 84 ® NanoPhotometer NP80/N60/N50/C40 User Guide Version 3.1.1 6. Baseline correction is set to off as default. Enabling the baseline corrections shows a list with different wavelength options: 377 nm, 604 nm, 650 nm, 770 nm (N/A N50) and 823 nm (N/A N50).

  • Page 85: Calculations

    ® NanoPhotometer NP80/N60/N50/C40 User Guide Version 3.1.1 11. Apply sample to the sample window and press the sample button to initiate the measurement. ALCULATIONS The absorbance ratio is calculated from the two path lengths specified by the user in the parameters.
  • Page 86 ® NanoPhotometer NP80/N60/N50/C40 User Guide Version 3.1.1 Note: There is no automatic path length setting in this method. Select either a virtual dilution of 15 (path length 0.67 mm) or of 140 (path length 0.07 mm). For cuvette application: Select the path length depending on the used cuvette. Options are: 0.5 mm, 1 mm, 2 mm, 5 mm and 10 mm For NanoVolume application with submicroliter cell (C40 only): Select Lid depending on your sample concentration (for concentration ranges of different...
  • Page 87 ® NanoPhotometer NP80/N60/N50/C40 User Guide Version 3.1.1 7. Unit selection 8. Baseline correction is set to off as default. Enabling the baseline corrections shows a list with different wavelength options: 377 nm, 604 nm, 650 nm, 770 nm (N/A N50) and 823 nm (N/A N50).

  • Page 88: Calculations

    ® NanoPhotometer NP80/N60/N50/C40 User Guide Version 3.1.1 10. Option to set/calculate a dilution factor for manual diluted samples. 11. Apply the blank ddH 0 or buffer to the illuminated sample window for the reference measurement and select blank to initiate the reading. Note: The illumination of the sample window can be switched off in the preferences.

  • Page 89: Nanophotometer Np80/N60/N50/C40

    ® NanoPhotometer NP80/N60/N50/C40 User Guide Version 3.1.1 In this method, the concentration of the sample is calculated based on the Beer-Lambert law given the user specified wavelength of interest and user defined extinction coefficient. The equations for calculating concentration without background correction are as follows: Without background correction: ��...
  • Page 90 ® NanoPhotometer NP80/N60/N50/C40 User Guide Version 3.1.1 For cuvette application: Select the path length depending on the used cuvette. For NanoVolume application with submicroliter cell (C40 only): Select Lid depending on your sample concentration (for concentration ranges of different lids see page 39 Submicroliter Cell Measurements Basics). Required sample volume for each lid is shown.
  • Page 91 ® NanoPhotometer NP80/N60/N50/C40 User Guide Version 3.1.1 6. Select the curve fit type: Options are linear regression, zero regression (forces the straight line through the origin) and 2 order regression. 7. Select Unit...

  • Page 92: Calculations

    ® NanoPhotometer NP80/N60/N50/C40 User Guide Version 3.1.1 8. Add up to 20 Concentrations by clicking on the Add Concentration button. Added concentrations can be deleted with Enter the concentrations of the standard curve. 9. Select Replicates none, 2 or 3 10.

  • Page 93: Custom Apps

    There is an option for designing customer specific Custom Apps which can be loaded to the ® NanoPhotometer . For more information about designing custom applications to suit individual research needs please contact Implen directly for assistance. TORED ESULTS The Stored Results icon opens a directory of folders containing files of results that have been previously saved.

  • Page 94: Stored Methods

    ® NanoPhotometer NP80/N60/N50/C40 User Guide Version 3.1.1 TORED ETHODS The stored methods icon opens the directories of folders containing methods stored by the user. ® On the left side of the screen all available directories/storages are shown: NanoPhotometer and/or USB flash drive. A click on a storage folder shows on the left side the subfolders of the storage folder and on the right side the method files.

  • Page 95: Preferences

    ® NanoPhotometer NP80/N60/N50/C40 User Guide Version 3.1.1 REFERENCES System preferences can be set by selecting preferences on the home screen. The preferences menu includes: General, Dyes, Warning Messages, Network, WiFi, Network Printer and Report Configuration. The menu item which is selected will have its preference options listed in the window to the right.

  • Page 96: About

    ® NanoPhotometer NP80/N60/N50/C40 User Guide Version 3.1.1 BOUT ® ® In About the following information of the NanoPhotometer are shown: NanoPhotometer Version, Serial Number, IP Address, Hardware Version, Firmware Version, Software Version, Time & Date of Initialization Test and Status of Self-test. ANGUAGE ®...

  • Page 97: Illumination Sample Window

    ® NanoPhotometer NP80/N60/N50/C40 User Guide Version 3.1.1 LLUMINATION AMPLE INDOW Toggle switch to switch on/off the illumination of the sample window (for NP80/N60/N50 only) Note: Only available for NP80/N60/N50 models. NABLE OLUME ® Toggle switch to enable the submicroliter cell option in the NanoPhotometer C40.

  • Page 98: Warning Messages

    ® NanoPhotometer NP80/N60/N50/C40 User Guide Version 3.1.1 Note: It is not possible to delete a dye from the default factory list; custom dyes can be deleted if they are not locked. It is possible to add a new dye to the list by selecting the button to add a new dye.

  • Page 99: Network

    ® NanoPhotometer NP80/N60/N50/C40 User Guide Version 3.1.1 ETWORK In Network preferences it is possible to choose between a Dynamic Host Configuration Protocol (DHCP) and a static network configuration. As default the DHCP protocol is selected. Connect ® the NanoPhotometer with the Ethernet and the IP is automatically set during the startup of the ®...

  • Page 100: W I F I

    ® NanoPhotometer NP80/N60/N50/C40 User Guide Version 3.1.1 The WiFi preferences allow the user to switch off the WiFi or to set up a WiFi Hotspot (WiFi Access Point) or a WiFi network. To set up a WiFi Hotspot select “WiFi Access Point” in the dropdown and click on the save icon. The WiFi Hotspot is needed to connect a computer via WiFi, an Android smartphone/tablet, an ®...

  • Page 101: Report Configuration

    ® NanoPhotometer NP80/N60/N50/C40 User Guide Version 3.1.1 ® Note: For printing on network printer the NanoPhotometer needs to be connected to the network either via Ethernet (LAN cable) or WiFi network. ® Note: If a printer is directly connected to the NanoPhotometer via USB, this printer will have the highest priority and will be the printer used by default when selecting Print on NanoPhotometer.

  • Page 102: Troubleshooting

    If the self-calibration test fails please contact the Implen Support Team. ESSAGES The software gives three different kind of messages confirmation (green), warning (yellow) and alert (red) messages.
  • Page 103 This message is shown on control devices like tablets and smartphones when no data has been saved in the control device folder. For Android Implen Folder is Empty tablets and smartphones this folder is always empty. Files are saved in the Download Folder of the Android tablet/smartphone directory.

  • Page 104: Important Alert Messages

    NP80/N60/N50/C40 User Guide Version 3.1.1 seconds. If the message still appears please create a log file and send it to Implen customer support. The measurement parameters for 0.3 µl NanoVolume samples read only the 0.07 mm path length (dilution 140). The utilized sample Sample concentration too low for 0.3µl - change...

  • Page 105: System Freeze

    Reboot the ® Server Not Available NanoPhotometer . If the message still appears please create a log file and send it to Implen Customer Support. Internal software communication problem during an open measurement session. Reboot the ®...

  • Page 106: Assistance

    Once the form is completed and the send button is selected a message will be sent directly to Implen and the appropriate support person will contact the end user as soon as possible to provide further support.

  • Page 107: Oftware Aintenance

    OFTWARE UPDATE Download the software update file from the Implen homepage: www.implen.de/downloads/ copy it to a USB flash drive. Note: Make sure that the sample name is NPOS.bin without any additions or extensions like from multiple downloads, otherwise rename file to be NPOS.bin exactly.

  • Page 108: Install Printer Driver

    Note: It is not possible to operate more than one printer at a time. EGAL The NPOS software is Copyright of Implen and its affiliates. The NPOS software includes some open source software components under the licenses listed in “Legal”.

  • Page 109: Contact Implen

    User Guide Version 3.1.1 ONTACT MPLEN There is an option to contact Implen for instruments connected to the internet. For any support issues and questions please contact the Implen team directly: Europe, Asia, South Pacific, Middle East and Africa Implen GmbH Schatzbogen 52 81829 München...

  • Page 110: Maintenance

    IQ/OQ documentation is also available. Please contact your local Implen office or an authorized Implen partner for further information. Support agreements that help to fulfill the demands of regulatory guidelines concerning...

  • Page 111: Cleaning And Generalc

    The warranty included in the conditions of supply is valid for 12 months and is valid only if the product has been used according to the instructions supplied. Implen or your supplier can accept no liability of loss or damage arising from the faulty or incorrect use of this product...

  • Page 112: Bezpečnostní Informace

    ® NanoPhotometer NP80/N60/N50/C40 User Guide Version 3.1.1 1- I PPENDIX NSTALACE PEKTROFOTOMETRU EZPEČNOSTNÍ INFORMACE Před zahájením instalace se prosím důkladně seznamte s výstražnými štítky a symboly na vašem přístroji a s jejich významem. Obsahují informace o tom, kde hrozí potenciální riziko a kde musíte být velmi obezřetní.

  • Page 113: Vybalení A Umístění

    NanoVolume. Zařízení musí být zapojeno do sítě pomocí napájecího zdroje / kabelu 60 W od společnosti Implen (se sadou baterií 90 W). Síťová zásuvka musí být vybavena ochranným vodičem (uzemněním). Lze používat se systémy napájecích zdrojů 90-250 V, 50-60 Hz.

  • Page 114: Veiligheidsinformatie

    ® NanoPhotometer NP80/N60/N50/C40 User Guide Version 3.1.1 2 - I PPENDIX NSTALLATIE PECTROFOTOMETER EILIGHEIDSINFORMATIE Voordat u begint met de installatie, moet u de tijd nemen om de waarschuwingslabels en symbolen op het instrument te bekijken en te leren wat deze betekenen. Deze zijn aangebracht om u te informeren indien er een gevaar is of indien u specifieke voorzorgen moet nemen.

  • Page 115: Uitpakken En Plaatsen

    Draag het instrument steeds door het hoofdgedeelte van het instrument vast te nemen en niet door bijvoorbeeld het optionele scherm of de NanoVolume-steun vast te nemen. De apparatuur moet zijn aangesloten met het 60W-netsnoer/voedingskabel door Implen meegeleverd (90W met batterijpack). Het stopcontact moet een aarding hebben. Het instrument is geschikt voor voedingen van 90-250 V, 50-60 Hz.

  • Page 116: Appendix 3 - I

    ® NanoPhotometer NP80/N60/N50/C40 User Guide Version 3.1.1 3 - I PPENDIX NSTALLATION DU SPECTROPHOTOMÈTRE NFORMATIONS DE SÉCURITÉ Avant de commencer l'installation, veuillez prendre le temps de vous familiariser avec les étiquettes et symboles de mise en garde de votre instrument, ainsi qu'avec leur signification. Ces étiquettes et symboles vous indiquent les situations dans lesquelles un risque potentiel existe, ou les cas où...
  • Page 117 L'équipement doit être relié à l'alimentation secteur à l'aide du câble/bloc d'alimentation 60 W fourni par Implen (90 W pour les modèles à batterie). La prise électrique doit être équipée d'un conducteur protecteur (mise à la terre). L'équipement peut être utilisé avec un système d'alimentation 90–250 V, 50–60 Hz.
  • Page 118 ® NanoPhotometer NP80/N60/N50/C40 User Guide Version 3.1.1 Mettez l'équipement sous tension à l'aide du bouton marche/arrêt qui se trouve sur son panneau arrière une fois que vous l'avez branché. L'instrument procédera à une série de vérifications d'autodiagnostic. Veuillez contacter immédiatement le fournisseur d'origine si vous rencontrez des difficultés techniques ou de manipulation d'échantillons.

  • Page 119: Auspacken Und Platzieren

    ® NanoPhotometer NP80/N60/N50/C40 User Guide Version 3.1.1 4 – I PPENDIX NSTALLATION PECTROPHOTOMETER ICHERHEITSINFORMATIONEN Vor Beginn der Installation nehmen Sie sich bitte die Zeit sich mit den Beschriftungen und Warnsymbolen auf Ihrem Instrument vertraut zu machen. Diese dienen als Information wo potenzielle Gefahren auftreten können bzw.
  • Page 120 Hauptgehäuse. Für den Betrieb des NanoPhotometers darf ausschließlich das 60W Netzteil / Kabel welches von Implen geliefert wurde verwendet werden (90 W mit Akku-Pack). Stromversorgung: 90 - 250 V, 50 - 60 Hz Für Mobile Versionen bitte den Akku vor dem ersten Gebrauch für mindestens 3 Stunden laden.

  • Page 121: Informazioni Di Sicurezza

    ® NanoPhotometer NP80/N60/N50/C40 User Guide Version 3.1.1 5 – I PPENDIX NSTALLAZIONE DELLO PETTROFOTOMETRO NFORMAZIONI DI SICUREZZA Prima di cominciare l’installazione, acquisire familiarità con i simboli e le etichette di avvertenza presenti sullo strumento e il loro significato. Simboli ed etichette segnalano potenziali pericoli oppure se occorre esercitare particolare cautela.

  • Page 122: Disimballaggio E Collocazione

    NanoVolume. L’apparecchio deve essere collegato all’alimentazione utilizzando il cavo di alimentazione/l’alimentatore da 60 watt fornito da Implen (90 watt con il gruppo batteria). La presa di alimentazione deve essere provvista di conduttore di protezione (terra/massa). Può...

  • Page 123: Nstalacja Spektrofotometru

    ® NanoPhotometer NP80/N60/N50/C40 User Guide Version 3.1.1 6 – I PPENDIX NSTALACJA PEKTROFOTOMETRU NFORMACJE DOTYCZĄCE BEZPIECZEŃSTWA Przed rozpoczęciem instalacji należy zapoznać się z etykietami ostrzegawczymi i symbolami umieszczonymi na urządzeniu oraz z ich znaczeniem. Informują one o możliwym zagrożeniu lub o konieczności zachowania szczególnej uwagi.
  • Page 124 NanoVolume. Urządzenie należy połączyć ze źródłem zasilania przewodem zasilania o mocy 60 W dostarczonym przez firmę Implen (90 W z akumulatorem). Gniazdo zasilania musi być uziemione. Urządzenie można stosować z systemami zasilania o napięciu 90–250 V, 50/60 Hz.
  • Page 125 ® NanoPhotometer NP80/N60/N50/C40 User Guide Version 3.1.1 W przypadku wystąpienia problemów technicznych lub z próbką należy niezwłocznie skontaktować się z pierwotnym dostawcą urządzenia. Uwaga: Eksploatacja urządzenia w sposób niezgodny ze specyfikacją lub w warunkach niesprzyjających bezpiecznemu działaniu może zmniejszyć skuteczność jego zabezpieczeń i doprowadzić...

  • Page 126: Spectrofotómetro

    ® NanoPhotometer NP80/N60/N50/C40 User Guide Version 3.1.1 7 – I PPENDIX NSTALACIÓN DEL SPECTROFOTÓMETRO NFORMACIÓN DE SEGURIDAD Antes de comenzar con la instalación, dedique un tiempo a familiarizarse con las etiquetas y símbolos de advertencia del instrumento y con su significado. Son para informarle cuando existan daños potenciales o se requiera una precaución particular.
  • Page 127 ® NanoPhotometer NP80/N60/N50/C40 User Guide Version 3.1.1 El símbolo situado sobre el producto, o en la documentación que acompaña al producto, indica que este dispositivo no debe ser tratado como residuo doméstico. Deberá llevarse al punto de recogida correspondiente para el reciclaje de equipos eléctricos y electrónicos. El desecho deberá...
  • Page 128 ® NanoPhotometer NP80/N60/N50/C40 User Guide Version 3.1.1 8 – S PPENDIX PEKTROFOTOMETERINSTALLATION ÄKERHETSINFORMATION Innan installationen påbörjas, var vänlig ägna tid åt att bekanta dig med ditt instruments varningsetiketter och symboler samt deras innebörd. De finns där för att informera dig om var det föreligger en risk eller särskild försiktighet krävs.
  • Page 129 NanoVolume-pelaren. Utrustningen måste vara ansluten till ström med 60 W strömförsörjning/sladd som levererats av Implen (90 W med batteripaket). Strömuttaget måste ha en skyddsledare (jord). Den kan användas med strömförsörjningssystem med 90-250 V, 50-60 Hz.
  • Page 130 ® NanoPhotometer NP80/N60/N50/C40 User Guide Version 3.1.1 9 – I PPENDIX NSTALAÇÃO DE SPECTROFOTÓMETRO NFORMAÇÕES DE EGURANÇA Antes de começar a instalação, procure familiarizar-se com os símbolos e as etiquetas de aviso no instrumento e o respetivo significado. Destinam-se a informá-lo sobre onde se encontram os potenciais perigos ou onde é...
  • Page 131 O equipamento deve ser ligado à corrente através do cabo elétrico de 60W fornecido pela Implen (90W no caso da bateria). A tomada de corrente deve dispor de um condutor de proteção (ligação à terra). Pode ser usado num sistema de alimentação elétrica de 90-250 V, 50-60 Hz.
  • Page 132 ® NanoPhotometer NP80/N60/N50/C40 User Guide Version 3.1.1 Nota: Se este equipamento for utilizado de forma não especificada ou em condições ambientais não adequadas para o funcionamento em segurança, a proteção proporcionada pelo equipamento pode ficar comprometida e a garantia do instrumento pode ser anulada.
  • Page 133 Blank Control............8, 98 IDS ................35 Bradford Assay............64 IDS files ..............35 Buttons ..............30 Illumination Sample Window ........97 Implen Document Source ......... 35 Installation Computer ............. 24 Cleaning ..............111 Multi-User ............24 Compatibility............. 23 Printer ..............26 Solvent ..............
  • Page 134 ® NanoPhotometer NP80/N60/N50/C40 User Guide Version 3.1.1 Positioning ............... 22 Collocazione ............122 Kinetics ..............69 Instalación ............127 Calculation ............71 Plaatsen ............. 115 Measurement Protocol ......... 69 Placering ............129 Platzieren ............119 Posicionamento ..........131 Positionnement ..........117 Lamp ..............
  • Page 135 ® NanoPhotometer NP80/N60/N50/C40 User Guide Version 3.1.1 Safety Information ............ 21 Table area ..............31 Bezpečnostní informace ........112 Touch Screen ............110 Información de seguridad ........126 Trademarks ............108 Informacje dotyczące bezpieczeństwa ....123 Troubleshooting ............. 102 Informações de Segurança ........ 130 Informations de sécurité...

This manual is also suitable for:

Nanophotometer n60Nanophotometer c40Nanophotometer n50

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