Implen NanoPhotometer N120 User Manual
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Implen NanoPhotometer N120 User Manual

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®
NanoPhotometer
N120/NP80/N60/N50/C40
User Manual
Version 4.3.1
Software Version 4.3.15670

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Summary of Contents for Implen NanoPhotometer N120

  • Page 1 ® NanoPhotometer N120/NP80/N60/N50/C40 User Manual Version 4.3.1 Software Version 4.3.15670...
  • Page 2 By using the NanoPhotometer device, End User hereby agrees to these terms, and agrees that Implen shall not be held liable for any loss, deletion or damage of any data or files for any reason, including any damages attributable thereto.
  • Page 3 N120/NP80/N60/N50/C40 User Manual Version 4.3.1 ® Declaration of conformity for the NanoPhotometer (C40/N50/N60/NP80/N120) ® This is to certify that the Implen NanoPhotometer conforms to the requirements of the following directives: 2014/35/EU Low Voltage Equipment Safety Directive 2014/30/EU Electromagnetic compatibility (EMC) directive...

  • Page 4: Table Of Contents

    ® NanoPhotometer N120/NP80/N60/N50/C40 User Manual Version 4.3.1 Contents ® ............... 9 HOTOMETER AT A LANCE ® ....................9 HOTOMETER VERVIEW ® N120 ....................... 10 HOTOMETER ® NP80 ......................11 HOTOMETER ® N60 ......................... 12 HOTOMETER ® N50 ......................... 13 HOTOMETER ®...
  • Page 5 ® NanoPhotometer N120/NP80/N60/N50/C40 User Manual Version 4.3.1 (N120) ............49 OLUME ULTI AMPLE EASUREMENT ASICS (C40/NP80) .................. 51 UVETTE EASUREMENT ASICS (C40)............... 52 UBMICROLITER EASUREMENTS ASICS ........................53 AMPLE ANDLING (N50/N60/N120/NP80/C40 ) ......55 OLVENT OMPATIBILITY WITH SUBMICROLITER CELL ....................56 RANSFER VIA ERVER ......................
  • Page 6 ® NanoPhotometer N120/NP80/N60/N50/C40 User Manual Version 4.3.1 ........................111 ETHOD VERVIEW ......................111 EASUREMENT ROTOCOL ..........................113 ALCULATIONS ..................114 ONCENTRATION ........................114 ETHOD VERVIEW ......................114 EASUREMENT ROTOCOL ..........................117 ALCULATIONS ..................118 TANDARD URVE ........................118 ETHOD VERVIEW ......................
  • Page 7 ® NanoPhotometer N120/NP80/N60/N50/C40 User Manual Version 4.3.1 ........................138 YSTEM REEZE ..................... 139 SSISTANCE ..........................139 UPPORT ......................139 EPORT ROBLEM ........................140 ANUAL ....................140 OFTWARE AINTENANCE NPOS ..........................140 UPDATE .......................... 141 REATE ..........................141 ACTORY ESET ..........................141 REATE ACKUP ........................
  • Page 8 ® NanoPhotometer N120/NP80/N60/N50/C40 User Manual Version 4.3.1 ..................160 ISIMBALLAGGIO E COLLOCAZIONE 6 – I 15. A ..........162 PPENDIX NSTALACJA PEKTROFOTOMETRU NFORMACJE DOTYCZĄCE BEZPIECZEŃSTWA ................162 .................. 163 OZPAKOWYWANIE I UMIEJSCOWIENIE 7 – I 16. A ........165 PPENDIX NSTALACIÓN DEL SPECTROFOTÓMETRO ....................

  • Page 9: Nano Photometer

    AT A LANCE ® HOTOMETER VERVIEW ® The Implen NanoPhotometer is a mobile, simple to use UV/Visible spectrophotometer with a CCD array detector with options ranging from NanoVolume to standard cuvette. The ® NanoPhotometer family comprises five different models. There are four single sample models available:...

  • Page 10: Nano Photometer ® N120

    ® NanoPhotometer N120/NP80/N60/N50/C40 User Manual Version 4.3.1 ® N120 HOTOMETER Item Number Touchscreen Battery Pack N120-Touch N120-Mobile...

  • Page 11: Nano Photometer ® Np80

    ® NanoPhotometer N120/NP80/N60/N50/C40 User Manual Version 4.3.1 ® NP80 HOTOMETER Item Number Touchscreen Battery Pack NP80 NP80-Touch NP80-Mobile...

  • Page 12: Nano Photometer ® N60

    ® NanoPhotometer N120/NP80/N60/N50/C40 User Manual Version 4.3.1 ® HOTOMETER Item Number Touchscreen Battery Pack N60-Touch N60-Mobile...

  • Page 13: Nano Photometer ® N50

    ® NanoPhotometer N120/NP80/N60/N50/C40 User Manual Version 4.3.1 ® HOTOMETER Item Number Touchscreen Battery Pack N50-Touch...

  • Page 14: Nano Photometer ® C40

    ® NanoPhotometer N120/NP80/N60/N50/C40 User Manual Version 4.3.1 ® HOTOMETER Item Number Touchscreen Battery Pack C40-Touch C40-Mobile...

  • Page 15: Instrument Rear Panel (N120/Np80/N60/N50/C40)

    ® NanoPhotometer N120/NP80/N60/N50/C40 User Manual Version 4.3.1 (N120/NP80/N60/N50/C40) NSTRUMENT ANEL ® To boot/shut down the NanoPhotometer push shortly (< 1 second) on the power on/off ® button at the back of the NanoPhotometer Note: A long push (> 3 seconds) initiate a hard reset. Only activate a hard reset of the ®...

  • Page 16: Accessories

    ® NanoPhotometer N120/NP80/N60/N50/C40 User Manual Version 4.3.1 CCESSORIES TANDARD CCESSORIES  Connecting Cable ® USB connecting cable to connect the NanoPhotometer to a computer to control the ® NanoPhotometer via computer (please see page 26 Software Installation). ®  NanoPhotometer Power Adapter ®...
  • Page 17 ® NanoPhotometer N120/NP80/N60/N50/C40 User Manual Version 4.3.1  Standard Solution (N120, NP80, N60, N50) ® The NanoPhotometer is recalibration free therefore it is not necessary to review the photometric accuracy on a regular basis. If the SOPs of a laboratory require a routine control of the photometric accuracy standard solutions can be used.
  • Page 18 ® NanoPhotometer N120/NP80/N60/N50/C40 User Manual Version 4.3.1  Field Kit Implen aluminum roller case for safe transportation, designed to fit in most airplane overhead bins; special compartments for all necessary accessories, cleaning tools and samples. ® Note: Delivered w/o NanoPhotometer and accessories ®...
  • Page 19 ® NanoPhotometer N120/NP80/N60/N50/C40 User Manual Version 4.3.1 Printout for DYMO LabelWriter 4XL Printout for DYMO LabelWriter 450 Note: The printouts are optimized for the label size of the DYMO LabelWriter 4XL. All other DYMO printers can be used. However, the font size will be zoomed to utilized paper size. ...

  • Page 20: Connectivity

    ® NanoPhotometer N120/NP80/N60/N50/C40 User Manual Version 4.3.1 ONNECTIVITY  USB A ® There is a USB A port on the front as well as the rear panel of the NanoPhotometer which is compatible with standard portable USB 2.0 storage devices (back) and USB 3.0 (front) for direct data transfer in a variety of formats including Excel.
  • Page 21 ® NanoPhotometer N120/NP80/N60/N50/C40 User Manual Version 4.3.1 Note: Due to the limitations of some handheld devices saving to a wireless device is limited ® to 40 measurements per dataset. Larger datasets can be saved on the NanoPhotometer itself.  HDMI ®...

  • Page 22: Nano Photometer

    ® NanoPhotometer N120/NP80/N60/N50/C40 User Manual Version 4.3.1 ® HOTOMETER PECIFICATIONS NanoVolume Performance Detection Range dsDNA N60, NP80: 1 - 16,500 ng/µl N50: 5 - 7,500 ng/µl N120: 2 - 8,000 ng/µl Detection Range BSA N60, NP80: 0.03 - 478 mg/ml N50: 0.15 - 217 mg/ml N120: 0.06 - 230 mg/ml Sample Volume...
  • Page 23 ® NanoPhotometer N120/NP80/N60/N50/C40 User Manual Version 4.3.1 N50 (Lid 15): < 0.004 A (0.67 mm path) @ 280 nm CV < 1% @ 0.3 – 1.5 A @ 280 nm < 0.004 A @ 0 – 0.3 A @ 280 nm N120 (Lid 10): CV <...

  • Page 24: Getting Started

    ® NanoPhotometer N120/NP80/N60/N50/C40 User Manual Version 4.3.1 ETTING TARTED PECTROPHOTOMETER NSTALLATION Note: Translations of the Safety Information and Unpacking and Positioning are available in Appendix 1 (Czech), Appendix 2 (Dutch), Appendix 3 (French), Appendix 4 (German), Appendix 5 (Italian), Appendix 6 (Polish), Appendix 7 (Spanish), Appendix 8 (Swedish) and Appendix 9 (Portuguese) AFETY NFORMATION...

  • Page 25: Unpacking And Positioning

    ® NanoPhotometer N120/NP80/N60/N50/C40 User Manual Version 4.3.1 regulatory and organization requirements before working with such potential infectious materials. Note: Do not spill any biological samples on instrument components. If spill occurs, disinfect the instrument immediately following your laboratory protocols and the cleaning instruction of the instrument (see page 145 Maintenance).

  • Page 26: Software Installation

    NPOS can store data either to a common directory or be configured to save to independent directories according to file format and/or instrument. NPOS can save data in an Implen .IDS format, PDF or as an Excel format file. ®...

  • Page 27: Installing Software On Computer

    NPOS software on your computer. 2. Start the NPOS installation file and follow the installation routine for single user installation. Installation file can be found on the Implen USB flash drive which is included with the ® NanoPhotometer at time of delivery or can be downloaded from the Implen webpage: www.implen.de/downloads.

  • Page 28: Installing Nano Photometer ® App On Tablet Or Smartphone

    ® NanoPhotometer N120/NP80/N60/N50/C40 User Manual Version 4.3.1 4. Start the NPOS software and select the desired connection. For a connection via ® USB cable, connect the NanoPhotometer to the PC using the USB cable supplied. For a connection via WiFi hotspot, ensure a stable WiFi connection between the PC ®...

  • Page 29: First Steps And Configuration Wizard

    IRST TEPS AND ONFIGURATION IZARD When starting the Implen NPOS the first time an Implen configuration wizard is shown. Please accept the End User License Agreement (EULA) and select the country in which the ® NanoPhotometer is used and confirm.

  • Page 30: Nano Photometer ® Basics

    ® NanoPhotometer N120/NP80/N60/N50/C40 User Manual Version 4.3.1 ® HOTOMETER ASICS ® The NanoPhotometer product line offers a complete solution for NanoVolume (N120/NP80/N60/N50/C40) and standard cuvette (C40/NP80) applications. The ® NanoPhotometer N120 is a multi-sample model that can measure up to 12 samples per run.
  • Page 31 ® NanoPhotometer N120/NP80/N60/N50/C40 User Manual Version 4.3.1 BCA (562 nm), Bradford (595 nm), Lowry (750 nm), and Biuret Protein Assays (546 nm; cuvette only) Assays Measures cell density at 600 nm (or in a range of 200 – 900 OD600 Stored Results Archive of stored results Stored Methods...

  • Page 32: Icons

    ® NanoPhotometer N120/NP80/N60/N50/C40 User Manual Version 4.3.1 CONS Icon Name Action WiFi Network WiFi network active; status of WiFi connection WiFi Hotspot WiFi hotspot active Assistance Opens the assistance page Battery Status Shows the actual battery status (only shown with optional battery pack) Preferences Opens the preferences page Home Screen...

  • Page 33: Buttons

    ® NanoPhotometer N120/NP80/N60/N50/C40 User Manual Version 4.3.1 UTTONS When opening a method and starting a sample measurement, a blank measurement is required. For the blank measurement, either water or ® sample buffer can be used to give the NanoPhotometer a reference of what zero should be.

  • Page 34: Measurement Screens

    ® NanoPhotometer N120/NP80/N60/N50/C40 User Manual Version 4.3.1 EASUREMENT CREENS INGLE AMPLE ETHODS  Side Tab Bar On the left side of the measurement screen there is a vertical tab bar that contains four tabs including: parameters, results, graph, and table. The different tabs allow the user to organize the measurement screen.
  • Page 35 ® NanoPhotometer N120/NP80/N60/N50/C40 User Manual Version 4.3.1 graph area. With the header tick box it is possible to select/unselect all samples (maximum number of sample selection is 30). The second column of the table indicates whether the measurement is saved ( ) or not saved (blank field). With the edit button the sample name of a single sample can be edited.

  • Page 36: Multi Sample Methods

    ® NanoPhotometer N120/NP80/N60/N50/C40 User Manual Version 4.3.1 ULTI AMPLE ETHODS  Change Screens Opening a multi sample method (Nucleic Acid, Protein UV, Protein Assay or Wavescan) shows the parameter screen first. To confirm the parameter swipe the screen to the left and the sample map is shown.
  • Page 37 ® NanoPhotometer N120/NP80/N60/N50/C40 User Manual Version 4.3.1 Selection Options Selection of cells is only possible in the sample map screen. Cell A01 must always be selected. A push in the top left corner selects/deselects all 96 cells at once: Manual selection is possible by selecting any area starting in cell A01. or by pushing in any cell:...
  • Page 38 ® NanoPhotometer N120/NP80/N60/N50/C40 User Manual Version 4.3.1 A push to the row/column header selects/deselects the whole row/column: Note: As a blank is necessary for each measurement it is not possible to select more cells as in the previous row/column. Sample ID Definition There are multiple options do define Sample IDs.
  • Page 39 Sample IDs can be imported with the Import function. For the import of Sample IDs , the sample IDs must first be defined in the following format and saved as a csv file. - for horizontal Sample Map: Note: Draft files are available for download on the Implen homepage (www.implen.de/downloads). - for vertical Sample Map:...
  • Page 40 NanoPhotometer N120/NP80/N60/N50/C40 User Manual Version 4.3.1 Note: Draft files are available for download on the Implen homepage (www.implen.de/downloads). Note: It is not possible to edit imported Sample IDs. To import sample IDs open the sample ID pop up and push the import button. A file directory is shown.
  • Page 41 ® NanoPhotometer N120/NP80/N60/N50/C40 User Manual Version 4.3.1 A push on a result cell opens a single result overview: A double click to a result cell shows tick boxes for all measured samples. The tick boxes can be used in the graph screen to overlay up to 12 samples. Up to 12 ticked samples will be overlaid by pushing on the graph icon ( ) in the navigation bar.

  • Page 42: Data Processing Dialogs

    ® NanoPhotometer N120/NP80/N60/N50/C40 User Manual Version 4.3.1 A table view ( ) is available on all result screens. The table lists all measured samples. With the edit button selected Sample IDs can be changed. ROCESSING IALOGS RINT Selecting the print icon ( ) opens a full screen dialog window with various print options.
  • Page 43 ® NanoPhotometer N120/NP80/N60/N50/C40 User Manual Version 4.3.1 ® the NanoPhotometer via USB cable. If no USB printer is available, the print command is sent to the defined network printer, if configured. Network printers can be configured in the preferences by entering the printer IP (see page 133 Network Printer). All ticked samples are printed.

  • Page 44: Save

    SD card crashes data loss cannot be ruled out. Implen Document Source File The Implen Document Source (IDS) file is a specific file format, which can only be opened with the NPOS software. It is a hardcopy file which cannot be changed. This file type contains all measurement information including raw data, results, values, and parameters.
  • Page 45 ® NanoPhotometer N120/NP80/N60/N50/C40 User Manual Version 4.3.1 Excel File Measurement data can be saved as Excel file. This file type contains all measurement information including raw data, results, and parameters. Note: The saved files contain only the selected samples at the time the file is saved. PDF File Measurement data can be saved as PDF file.

  • Page 46: Delete

    ® NanoPhotometer N120/NP80/N60/N50/C40 User Manual Version 4.3.1 ELETE Selecting the delete icon ( ) opens a full screen overlay dialog window. All data which are selected (tick) in the first table column will be deleted. The header tick box selects/deselects all samples.

  • Page 47: Store Methods

    ® NanoPhotometer N120/NP80/N60/N50/C40 User Manual Version 4.3.1 TORE ETHODS With the favorite icon it is possible in each method to save the parameter settings for easy access of custom defined methods. Select the desired parameter settings and open the Stored Method dialog by pushing o the favorite icon Enter a method name and select a save location in the folder.

  • Page 48: Basic Operation

    5 mm, 2 mm, 1 mm or 0.5 mm and a center height of 8.5 cm. ® Note: NanoVolume application in the NanoPhotometer C40 is only available with the optional accessory “submicroliter cell” from Implen. (N50/N60/NP80) OLUME INGLE AMPLE...

  • Page 49: Nano Volume Multi Sample

    Note: Do not use aggressive solvents such as strong acids or bases or organic solvents at any time (see page 55 Solvent Compatibility (N50/N60/N120/NP80/C40 with submicroliter cell). If unsure please contact support@implen.de for detailed information about your specific reagent/solvent. 7. It is possible to enter a sample name for each sample in the input window “enter sample name”.
  • Page 50 Note: Do not use aggressive solvents such as strong acids or bases or organic solvents at any time (see page 55 solvent compatibility). If unsure please contact support@implen.de for detailed information about your specific reagent/solvent. Raise the lid arm and pipette 2µl of sample solution onto the illuminated sample windows.

  • Page 51: Cuvette Measurementb

    ® NanoPhotometer N120/NP80/N60/N50/C40 User Manual Version 4.3.1 (C40/NP80) UVETTE EASUREMENT ASICS ® The NanoPhotometer (NP80 and C40 only) is compatible with standard cuvettes having an 8.5 mm center height. The light path is indicated with a red status LED arrow for the NP80 model and with two white arrows for the C40 model.

  • Page 52: Submicroliter Cellm

    C40. Insert the submicroliter cell to the cell holder with the cell windows facing the light beam. We recommend facing the Implen logo to the right side. The light beam is directed from BACK to FRONT as indicated with the white arrows.

  • Page 53: Sample Handling Tips

    Note: Do not use aggressive solvents such as strong acids or bases or organic solvents at any time (see page 55 solvent compatibility). If unsure please contact support@implen.de for detailed information about your specific reagent/solvent. 8. It is possible to enter a sample name for each sample in the input window “enter sample name”.
  • Page 54 ® NanoPhotometer N120/NP80/N60/N50/C40 User Manual Version 4.3.1 highly concentrated samples or certain proteins, the recommended procedure for cleaning is to use a slightly wet lint-free laboratory wipe (with water or 70% EtOH depending on sample type) to thoroughly clean the sample surface. ...

  • Page 55: Solvent Compatibility

    Note: Highly concentrated acids and bases are not recommended. It is recommended to wipe the sample surface with a lint-free laboratory wipe immediately upon completion of each measurement. For more information about compatibility of specific solvents not listed above, please contact the Implen support team (support@implen.de) to check the compatibility.

  • Page 56: Data Transfer Via Files

    ® NanoPhotometer N120/NP80/N60/N50/C40 User Manual Version 4.3.1 RANSFER VIA ERVER ® All data saved on the NanoPhotometer can easily be accessed from and transferred to a ® computer via the NanoPhotometer file server. Connection options are LAN/WLAN, USB cable or WiFi Hotspot. It is possible to create user accounts for password secured file server access.

  • Page 57: Battery Operation

    ® NanoPhotometer N120/NP80/N60/N50/C40 User Manual Version 4.3.1  File Server Access via USB cable ® For file server access via USB cable connection, connect the NanoPhotometer with a USB A/B cable to the computer and open the Windows Explorer or Connect to Server option for Mac (see file server access via LAN/WLAN) and enter \\192.168.7.1\ for connection.

  • Page 58: Nano Photometer

    ® NanoPhotometer N120/NP80/N60/N50/C40 User Manual Version 4.3.1 ® HOTOMETER PPLICATIONS ® The NanoPhotometer comes with pre-programmed applications as well as the ability to create custom applications. Any application method can be selected by tapping the icon once or pushing on the icon (computer based software). Note: Within each method selection of NanoVolume or cuvette mode (NP80/C40) is possible.
  • Page 59 ® NanoPhotometer N120/NP80/N60/N50/C40 User Manual Version 4.3.1 4. For NanoVolume application: Select the volume of sample to be applied. Note: 1 - 2 µl (default): automatic path length setting; 0.3 µl measures only the 0.07 mm path length (for samples with concentrations > 420 ng/µl dsDNA) For cuvette application: Select the path length depending on the used cuvette.
  • Page 60 ® NanoPhotometer N120/NP80/N60/N50/C40 User Manual Version 4.3.1 Note: A new Blank is recommended when changing the dilution lid. 5. Select the Units in which the concentration should be calculated. Options are ng/µl (default), µg/µl and µg/ml. pmol/µl if a nucleic acid sequence is entered for nucleic acid factor calculation.
  • Page 61 ® NanoPhotometer N120/NP80/N60/N50/C40 User Manual Version 4.3.1 9. Option to set/calculate a dilution factor for manual diluted samples. 10. Apply the blank ddH 0 or buffer to the illuminated sample window on the pedestal and close the lid arm for the reference measurement and select blank to initiate the reading.

  • Page 62: Measurement Protocol

    ® NanoPhotometer N120/NP80/N60/N50/C40 User Manual Version 4.3.1 Note: Auto sample function is only available NanoVolume methods and ® new NanoPhotometer versions it may not be available after updating an older firmware version. (N120) EASUREMENT ROTOCOL ULTI AMPLE 1. Select the Nucleic Acids icon on the home screen. To change between single and multi-sample application use the toggle switch Single Sample in the parameter area.
  • Page 63 ® NanoPhotometer N120/NP80/N60/N50/C40 User Manual Version 4.3.1 also possible to add any wavelength in the range of 220 – 350 nm 6. Air bubble recognition is disabled by default. Set to on it detects air bubbles, lint residues and bad sample conditions of the sample. Note: Lint residues and bad sample conditions are detected even if the air bubble recognition is set to off.
  • Page 64 ® NanoPhotometer N120/NP80/N60/N50/C40 User Manual Version 4.3.1 There are two options: Horizontally: Measure up to 12 samples (A01 – A12 shown in sample map as row) Vertically: Measure up to 8 samples (A01 – H01 shown in sample map as column) 9.

  • Page 65: Calculations

    ® NanoPhotometer N120/NP80/N60/N50/C40 User Manual Version 4.3.1 To measure a defined amount of samples select a cell pattern starting at A01. Sample IDs can be defined by pushing on the sample ID icon ( ) in the navigation bar. The opened pop up offers options to define, import, export or delete sample IDs. Further information about cell selection and sample ID definition can be found in chapter 3 ®...
  • Page 66 ® NanoPhotometer N120/NP80/N60/N50/C40 User Manual Version 4.3.1 nucleic acid samples can be calculated with or without background correction depending on enabled/disabled background correction parameter. Without background correction: C = A * ε * Đ With background correction: C = ( A ) * ε...
  • Page 67 ® NanoPhotometer N120/NP80/N60/N50/C40 User Manual Version 4.3.1 Concentration in ng/µl Absorbance at 260 nm (10 mm path) Absorbance at selected background wavelength (10 mm path) Absorbance value at the absorbance maximum of the dye (10 mm path) max, dye ε Extinction coefficient/nucleic acid factor in ng*cm/µl Dye-dependent correction factor at 260 nm Đ...
  • Page 68 ® NanoPhotometer N120/NP80/N60/N50/C40 User Manual Version 4.3.1 With background correction without dye correction: 324.5 * (A − A max, dye FOI = ε * 10 * (A − A ) * ε Without background correction and with dye correction: 324.5 * A max, dye FOI = ε...
  • Page 69 ® NanoPhotometer N120/NP80/N60/N50/C40 User Manual Version 4.3.1 NP80/N60/N50/C40 Oyster-500 78,000 0.29 NP80/N60/N50/C40 Oyster-550 150,000 0.05 NP80/N60/N50/C40 Oyster-556 155,000 0.03 NP80/N60/C40 Oyster-645 220,000 0.05 NP80/N60/C40 Oyster-650 200,000 0.04 NP80/N60/C40 Oyster-656 200,000 0.04 NP80/N60/N50/C40 Texas Red 85,000 0.23  Ratios Reactions utilizing nucleic acids often require minimum purity standards. Common contaminants of nucleic acid samples include: proteins, organic compounds, and other.

  • Page 70: Protein Uv

    ® NanoPhotometer N120/NP80/N60/N50/C40 User Manual Version 4.3.1 ROTEIN ETHOD VERVIEW The Protein UV method exploits the inherent absorbance of proteins at 280 nm in combination with the Beer-Lambert Law, where each protein is characterized by a protein specific extinction coefficient (ε) which can be used to determine total protein concentration of a solution.
  • Page 71 ® NanoPhotometer N120/NP80/N60/N50/C40 User Manual Version 4.3.1 EASUREMENT ROTOCOL INGLE AMPLE 1. Select the Protein UV icon on the home screen. 2. To change between NanoVolume and Cuvette application (NP80/C40 only) use the Change to Cuvette/NanoVolume button below the parameter area. 3.
  • Page 72 ® NanoPhotometer N120/NP80/N60/N50/C40 User Manual Version 4.3.1 Options are: 0.5 mm, 1 mm, 2 mm, 5 mm and 10 mm If it is desired to heat the sample to 37°C use the toggle switch to turn on cell holder heater. When the cuvette holder has reached 37°C the toggle color changes to green.
  • Page 73 ® NanoPhotometer N120/NP80/N60/N50/C40 User Manual Version 4.3.1 8. Air bubble recognition is disabled by default. When enabled it detects air bubbles, lint residues and poor conditions of the sample. Note: Lint residues and bad sample conditions are detected even if the air bubble recognition is set to off.
  • Page 74 ® NanoPhotometer N120/NP80/N60/N50/C40 User Manual Version 4.3.1 11. Apply the blank ddH 0 or buffer to the illuminated sample window on pedestal for the reference measurement and select blank to initiate the reading. Note: The illumination of the sample window can be switched off in the preferences.
  • Page 75 ® NanoPhotometer N120/NP80/N60/N50/C40 User Manual Version 4.3.1 Options are: BSA, SA Mouse, SA Human, IgG Mouse, IgG Human, IgE Human, Lysozyme, OD1, Custom (Molar Extinction Coefficient), Custom (Extinction Coefficient) and Custom (1/Ɛ).  For Custom (Mol. Ext. Coefficient) enter Molecular Weight in g/mol and the Mol. Ext. Coefficient in M-1*cm-1 ...
  • Page 76 ® NanoPhotometer N120/NP80/N60/N50/C40 User Manual Version 4.3.1 7. Air bubble recognition is disabled by default. When activated it detects air bubbles, lint residue and bad sample conditions. Note: Lint residue and bad sample conditions are detected even if the air bubble recognition is off.
  • Page 77 ® NanoPhotometer N120/NP80/N60/N50/C40 User Manual Version 4.3.1 Vertically: Measure up to 8 samples (A01 – H01 shown in sample map as column) 10. To confirm the parameter setting, swipe the screen to the left and the sample map screen is shown. The sample map offers the possibility to select cells and define sample IDs for measurements.

  • Page 78: Measurement Protocol

    ® NanoPhotometer N120/NP80/N60/N50/C40 User Manual Version 4.3.1 The opened pop up offers options to define, import, export or delete sample IDs. Further information about cell selection and sample ID definition can be found in chapter 3 ® NanoPhotometer Basics / Sample Map on page 36. 11.
  • Page 79 ® NanoPhotometer N120/NP80/N60/N50/C40 User Manual Version 4.3.1 extinction coefficient defined by the user. The protein concentration is calculated with or without background correction as follows: With background correction: C = ( A − A ) * ε * Ð prot Without background correction: C = A * ε...
  • Page 80 ® NanoPhotometer N120/NP80/N60/N50/C40 User Manual Version 4.3.1 C = A * ε * Ð prot Concentration in mg/ml Absorbance at 280 nm (10 mm path) Absorbance at selected background wavelength (10 mm path) Absorbance value at the absorbance maximum of the dye (10 mm path) max, dye ε...
  • Page 81 ® NanoPhotometer N120/NP80/N60/N50/C40 User Manual Version 4.3.1...
  • Page 82 ® NanoPhotometer N120/NP80/N60/N50/C40 User Manual Version 4.3.1 W/o background and with dye correction: * ε max, dye prot DOL = − cf ) * ε max, dye W/o background and w/o dye correction: * ε max, dye prot DOL = * ε...
  • Page 83 ® NanoPhotometer N120/NP80/N60/N50/C40 User Manual Version 4.3.1  Ratios Protein samples e.g. from whole cell lysates may contain nucleic acids. To check the purity of the isolated protein, the 260/280 ratio is calculated to give an indication of the nucleic acid contamination.

  • Page 84: Protein Assays

    ® NanoPhotometer N120/NP80/N60/N50/C40 User Manual Version 4.3.1 ROTEIN SSAYS ETHOD VERVIEW Protein concentration may be measured using colorimetric assays, in which certain reagents are added to the protein solution to generate a colored product; either a protein-cupric ion chelate as in the Biuret, Lowry, BCA assays or a protein-dye complex as in the Bradford assay.
  • Page 85 ® NanoPhotometer N120/NP80/N60/N50/C40 User Manual Version 4.3.1 EASUREMENT ROTOCOL INGLE AMPLE 1. Select protein assays icon from home screen. To change between NanoVolume and cuvette application, use the Change to Cuvette/NanoVolume button below the parameter area (NP80/C40 only). 3. To change the assay type push on Bradford and a list with available options opens on the right side.
  • Page 86 ® NanoPhotometer N120/NP80/N60/N50/C40 User Manual Version 4.3.1 For NanoVolume application with submicroliter cell (C40 only): Select Lid depending on your sample concentration (for concentration ranges of different lids see page 52 Submicroliter Cell Measurements Basics). Required sample volume for each lid is shown. Note: A new Blank is recommended when changing the dilution lid.
  • Page 87 ® NanoPhotometer N120/NP80/N60/N50/C40 User Manual Version 4.3.1 7. Select Unit 8. Add up to 20 Concentrations by pushing on the Add Concentration button. Added concentrations can be deleted with Enter the concentrations of the standards for the standard curve. 9. Select Replicates none, 2 or 3...
  • Page 88 ® NanoPhotometer N120/NP80/N60/N50/C40 User Manual Version 4.3.1 10. Measure a blank and depending on the replicate selection, all required concentrations. Absorbances of the replicates will be shown in the results area and if replicates are selected a mean value for each standard. It is possible to exclude single measurements from the curve calculation by switching the toggle switch off.
  • Page 89 ® NanoPhotometer N120/NP80/N60/N50/C40 User Manual Version 4.3.1 instrument version: BCA default value 750 nm Bradford default value 750 nm Lowry default value 405 nm Note: It is recommended to use the default baseline for each assay. 5. Select curve fit type: Options are linear regression, zero regression (forces the straight line through the origin) and 2 order regression 6.
  • Page 90 ® NanoPhotometer N120/NP80/N60/N50/C40 User Manual Version 4.3.1 8. Select Replicates none, 2 or 3 9. Select sample loading orientation. 10. To confirm the parameter setting, swipe the screen to the left and the standard map screen is shown. The standard map is created automatically depending on the parameter settings (sample orientation, concentration and replicates).
  • Page 91 ® NanoPhotometer N120/NP80/N60/N50/C40 User Manual Version 4.3.1 e.g. vertically 6 standards and 3 replicates defined: Note: It is necessary to measure all defined blanks (horizontally 12 and vertically 8) as they might be needed for sample measurements. Measure blanks and standards as defined by initiating the blank button for blank measurements and sample button for the standard measurements.
  • Page 92 ® NanoPhotometer N120/NP80/N60/N50/C40 User Manual Version 4.3.1 The sample map offers the possibility to select cells and define sample IDs for measurements. It is not necessary to make a selection. If cells are left empty without defined sample IDs for horizontal and vertical sample loading, all 12 positions for horizontal loading and all 8 positions for vertical loading are measured.

  • Page 93: Saving And Loadings

    ® NanoPhotometer N120/NP80/N60/N50/C40 User Manual Version 4.3.1 ® new NanoPhotometer versions it may not be available after updating an older firmware version. Note: Once the sample measurement is initiated it is no longer possible to change the standard curve. AVING AND OADING TANDARD URVES...

  • Page 94: Kinetics

    ® NanoPhotometer N120/NP80/N60/N50/C40 User Manual Version 4.3.1 INETICS ETHOD VERVIEW The Kinetics application is useful for: measuring initial rates of enzyme-catalyzed reactions, performing progress-curve analysis for complete reactions, calculating basic Michaelis- Menten parameters for single-substrate reactions, and measuring enzyme inhibition. Simple kinetics studies, where the change in absorbance is followed as a function of time at a fixed ®...
  • Page 95 ® NanoPhotometer N120/NP80/N60/N50/C40 User Manual Version 4.3.1 Note: Only available for cuvette applications (NP80 and C40). For NanoVolume Application: Select the dilution depending on the sample concentration. Note: There is no automatic path length setting available in this method. Select either a virtual dilution of 15 (path length 0.67 mm) or of 140 (path length 0.07 mm).

  • Page 96: Calculations

    ® NanoPhotometer N120/NP80/N60/N50/C40 User Manual Version 4.3.1 6. Insert cuvette with the reference sample and select blank button to initiate the measurement. 7. Insert cuvette with the sample and select the sample button to initiate the measurements. Once the kinetic is started the Blank button turns to a Pause/Continue button and the Sample button to a Stop button.

  • Page 97: Od600

    For more information see Technical Note #8 OD 600 which can be downloaded on the Implen webpage: www.implen.de/scientific-publications/ A calibration curve can be constructed by comparing measured OD 600 to expected OD 600.

  • Page 98: Measurement Protocol

    ® NanoPhotometer N120/NP80/N60/N50/C40 User Manual Version 4.3.1 Figure 1 Bacterial growth curve EASUREMENT ROTOCOL 1. Select the OD600 icon on the home screen To change between NanoVolume and cuvette application, use the Change to Cuvette/NanoVolume button below the parameter area (NP80/C40 only).
  • Page 99 ® NanoPhotometer N120/NP80/N60/N50/C40 User Manual Version 4.3.1 For NanoVolume application with submicroliter cell (C40 only): Select Lid depending on your sample concentration (for concentration ranges of different lids see page 52 Submicroliter Cell Measurements Basics). Required sample volume for each lid is shown. Note: A new Blank is recommended when changing the dilution lid.

  • Page 100: Calculations

    ® NanoPhotometer N120/NP80/N60/N50/C40 User Manual Version 4.3.1 8. Option to set/calculate a dilution factor for manual diluted samples. 9. Insert cuvette with the reference sample and select blank button to initiate the measurement 10. Insert cuvette with the sample and select the sample button to initiate the measurement.

  • Page 101: More Apps

    ® NanoPhotometer N120/NP80/N60/N50/C40 User Manual Version 4.3.1 The More Apps icon located on the home screen opens another menu screen with access to ® icons for additional applications available on the NanoPhotometer . The applications featured in this menu include: wavelength, concentration, wavescan, absorbance/ratio, standard curve and custom applications.
  • Page 102 ® NanoPhotometer N120/NP80/N60/N50/C40 User Manual Version 4.3.1 If it is desired to heat the sample to 37°C use the toggle switch to turn on cell holder heater. When the cuvette holder has reached 37°C the toggle color changes to green. Note: Only available for cuvette applications (NP80 and C40).
  • Page 103 ® NanoPhotometer N120/NP80/N60/N50/C40 User Manual Version 4.3.1 6. Option to smooth the graph with different boxcars. Options: Off, 1 = boxcar 11 (default), 2 = boxcar 21 and 3 = boxcar 61 7. Option to enter formulas to calculate It is possible to enter up to 5 different formulas for result calculation. Input options are any number with up to four significant figures (use period “.”...

  • Page 104: Calculations

    ® NanoPhotometer N120/NP80/N60/N50/C40 User Manual Version 4.3.1 10. Use a lint-free laboratory wipe to clean both the sample window on pedestal and mirror in lid arm prior to applying the next sample. Note: It can be helpful to apply the blank a second time and read it as a sample to ensure a proper blank.

  • Page 105: More Apps : Wavescan Method Overview

    ® NanoPhotometer N120/NP80/N60/N50/C40 User Manual Version 4.3.1 AVESCAN ETHOD VERVIEW Using the wavescan application it is possible to obtain the complete spectral scan for a defined wavelength range between 200-900 nm (NP80/N60/C40) or from 200-650 nm (N50). EASUREMENT ROTOCOL INGLE AMPLE 1.
  • Page 106 ® NanoPhotometer N120/NP80/N60/N50/C40 User Manual Version 4.3.1 Note: A new Blank is recommended when changing the dilution lid. 4. Set Start and End Wavelength to define the scan range. Note: If samples with a different wavelength range are selected, the graphs are shown on full scan range of 200–900 nm (N50: 200-650 nm).
  • Page 107 ® NanoPhotometer N120/NP80/N60/N50/C40 User Manual Version 4.3.1 8. Apply the blank ddH 0 or buffer to the illuminated sample window on pedestal for the reference measurement and select blank to initiate the reading. Note: The illumination of the sample window can be switched off in the preferences.
  • Page 108 ® NanoPhotometer N120/NP80/N60/N50/C40 User Manual Version 4.3.1 4. Set Start and End Wavelength to define the scan range. 5. Baseline correction is set off by default. Enabling the baseline corrections shows a list with different wavelength options: 377 nm, 604 nm, 650 nm, 770 nm and 823 nm. Option to enter any wavelength between 200 nm and 900 nm.
  • Page 109 ® NanoPhotometer N120/NP80/N60/N50/C40 User Manual Version 4.3.1 Vertically: Measure up to 8 samples (A01 – H01 shown in sample map as column) 7. To confirm the parameter setting, swipe the screen to the left and the sample map screen is shown. The sample map offers the possibility to select cells and define sample IDs for measurements.

  • Page 110: Measurement Protocol Measurement Protocol Calculations

    ® NanoPhotometer N120/NP80/N60/N50/C40 User Manual Version 4.3.1 Further information about cell selection and sample ID definition can be found in chapter 3 ® NanoPhotometer Basics / Sample Map on page 36. 8. Apply the blank ddH20 or buffer to the illuminated sample windows on pedestal for the reference measurement and select blank to initiate the reading.

  • Page 111: Ratio

    ® NanoPhotometer N120/NP80/N60/N50/C40 User Manual Version 4.3.1 BSORBANCE ATIO ETHOD VERVIEW In this mode, it is possible to determine simple absorbance ratios for a given sample by measuring the absorbance at two wavelengths specified in the parameters of the method relative to a blank.
  • Page 112 ® NanoPhotometer N120/NP80/N60/N50/C40 User Manual Version 4.3.1 Note: A new Blank is recommended when changing the dilution lid. 4. Enter desired wavelengths ( 1-1 and  1-2) for ratio calculation. It is possible to measure up to 20 absorbance/ratios simultaneously. More wavelengths for ratio calculation can be added by selecting the Add Ratio button.

  • Page 113: Calculations

    ® NanoPhotometer N120/NP80/N60/N50/C40 User Manual Version 4.3.1 8. Apply the blank ddH20 or buffer to the illuminated sample window on pedestal for the reference measurement and select blank to initiate the reading. Note: The illumination of the sample window can be switched off in the preferences.

  • Page 114: More Apps : Concentration

    ® NanoPhotometer N120/NP80/N60/N50/C40 User Manual Version 4.3.1 ONCENTRATION ETHOD VERVIEW In this mode, concentration can be calculated for a sample by determining the absorbance at a specific wavelength relative to a reference. The concentration is then obtained by multiplying the measured absorbance by a specific factor. This factor may be known in advance and entered by the user, or it may be calculated by the instrument by measuring a set of standard (standard curve method) with known concentrations to create a standard curve.
  • Page 115 ® NanoPhotometer N120/NP80/N60/N50/C40 User Manual Version 4.3.1 lids see page 52 Submicroliter Cell Measurements Basics). Required sample volume for each lid is shown. Note: A new Blank is recommended when changing the dilution lid. 4. Default wavelength is 260 nm but can be changed in the range of 200- 900 nm (N50: 200–650 nm), depending on the sample/application.
  • Page 116 ® NanoPhotometer N120/NP80/N60/N50/C40 User Manual Version 4.3.1 8. Option to smooth the graph with different boxcars. Options: Off, 1 = boxcar 11 (default), 2 = boxcar 21 and 3 = boxcar 61 9. Option to set/calculate a dilution factor for manual diluted samples. 10.

  • Page 117: Calculations

    ® NanoPhotometer N120/NP80/N60/N50/C40 User Manual Version 4.3.1 11. Use a lint-free laboratory wipe to clean both the sample window on pedestal and mirror in lid arm prior to applying the next sample. Note: It can be helpful to apply the blank a second time and read it as a sample to ensure a proper blank.

  • Page 118: More Apps : Standard Curve

    ® NanoPhotometer N120/NP80/N60/N50/C40 User Manual Version 4.3.1 TANDARD URVE ETHOD VERVIEW The construction of a calibration curve from multiple standards of known concentrations can ® be created and stored on the NanoPhotometer . The standard curve can be used to quantify samples of the same type with unknown concentrations.
  • Page 119 ® NanoPhotometer N120/NP80/N60/N50/C40 User Manual Version 4.3.1 Select Lid depending on your sample concentration (for concentration ranges of different lids see page 52 Submicroliter Cell Measurements Basics). Required sample volume for each lid is shown. Note: A new Blank is recommended when changing the dilution lid. 4.
  • Page 120 ® NanoPhotometer N120/NP80/N60/N50/C40 User Manual Version 4.3.1 7. Add up to 20 Concentrations by pushing on the Add Concentration button. Added concentrations can be deleted with Enter the concentrations of the standard curve. 8. Select Replicates none, 2 or 3 9.

  • Page 121: Calculations

    USTOM There is an option for designing customer specific Custom Apps which can be loaded to the ® NanoPhotometer . For more information about designing custom applications to suit individual research needs please contact Implen directly for assistance.

  • Page 122: Stored Results

    ® NanoPhotometer N120/NP80/N60/N50/C40 User Manual Version 4.3.1 TORED ESULTS The Stored Results icon opens a directory of folders containing files of results that have been previously saved. On the left side of the screen all available directories/storages are shown: ® NanoPhotometer , Control Device, Network and/or USB flash drive (depending on availability).

  • Page 123: Stored Methods

    ® NanoPhotometer N120/NP80/N60/N50/C40 User Manual Version 4.3.1 TORED ETHODS The stored methods icon opens the directories of folders containing methods stored by the user. On the left side of the screen all available directories/storages are shown: ® NanoPhotometer , Control Device, Network and/or USB flash drive (depending on availability).

  • Page 124: Preferences

    ® NanoPhotometer N120/NP80/N60/N50/C40 User Manual Version 4.3.1 REFERENCES System preferences can be set by selecting preferences on the home screen. The preferences menu includes: General, Dyes, Warning Messages, Network, Printer and CFR21. The preference options of the selected menu item are listed in the window on the right.

  • Page 125: Display

    ® NanoPhotometer N120/NP80/N60/N50/C40 User Manual Version 4.3.1 Note: Do not change the date and time at the same time as the time zone. Make sure that the UTC time is set correctly before changing the time zone. ISPLAY Brightness: adjustment of the built-in screen brightness Screensaver: Option to switch the screensaver video on and off.

  • Page 126: Illumination Sample Window

    ® NanoPhotometer N120/NP80/N60/N50/C40 User Manual Version 4.3.1 Note: The change will affect all methods. Note: It is possible to work in users’ desired language and change the selected language for Excel and PDF Printouts to English. This option can be selected with the toggle switches Excel in English and PDF/Printing in English.
  • Page 127 ® NanoPhotometer N120/NP80/N60/N50/C40 User Manual Version 4.3.1 Each dye has either a lock icon ( ) in front of the dye name indicating that the dye is locked and cannot be changed or a delete symbol ( ). The delete option is only available for unlocked and not preprogrammed dyes.

  • Page 128: Warning Messages

    ® NanoPhotometer N120/NP80/N60/N50/C40 User Manual Version 4.3.1 ARNING ESSAGES LANK ONTROL Toggle switch to switch on/off the Blank Control™ of the NanoPhotometer ® Note: Blank Control™ is available for all NanoVolume methods (for N120/NP80/N60/N50). AMPLE UALITY ONTROL It is possible to change the upper and the lower limit of the ratio alert warning messages. Default values for nucleic acid ratios are: 260/230 ratio 1.8 A - 3 A and 260/280 ratio 1.65 A - 2.5 A.

  • Page 129: Network

    ® NanoPhotometer N120/NP80/N60/N50/C40 User Manual Version 4.3.1 ETWORK Selecting Network in the Preferences menu opens a window to the right of the preferences menu with the following options: Network Settings, WLAN Settings, File Server Access and Network Folder. ETWORK ETTINGS In Network Settings it is possible to choose between a Dynamic Host Configuration Protocol (DHCP) and a static network configuration.

  • Page 130: Wlan Settings

    ® NanoPhotometer N120/NP80/N60/N50/C40 User Manual Version 4.3.1 For static IP configuration select Static in the Configure IPv4 dropdown and enter the IPv4 address, netmask and gateway then confirm with disk icon ( ). WLAN S ETTINGS The WLAN Setting preferences allow the user to switch off the WiFi or to set up a WiFi Hotspot (WiFi Access Point) or a WiFi network.

  • Page 131: Ile Erver Ccess

    ® NanoPhotometer N120/NP80/N60/N50/C40 User Manual Version 4.3.1 ERVER CCESS The File Server Access preferences allow creating user accounts to limit the access to the ® NanoPhotometer file server via network. By default, access to the file server is open to any user.

  • Page 132: Network Folder

    ® NanoPhotometer N120/NP80/N60/N50/C40 User Manual Version 4.3.1 ETWORK OLDER The network folder preferences allow creating network folder for saving data directly from the ® NanoPhotometer to a network folder. To add a network folder push the + icon and enter network folder nickname and network path of the network folder.

  • Page 133: Printer

    ® NanoPhotometer N120/NP80/N60/N50/C40 User Manual Version 4.3.1 RINTER Selecting Printer in the Preferences menu opens a window to the right of the preferences menu with the following options: Network Printer and Report Configuration. ETWORK RINTER ® It is possible to print via network (Ethernet) or Airprint / IPP compatible (supporting PDF format) printers in the network.

  • Page 134: Report Configuration

    In the CFR21 Preferences it is possible to enable the optional CFR21 Software. This is only possible with a license key. For further details please contact the Implen Support team or refer to the CFR21 software user manual.

  • Page 135: Troubleshooting

    If the ok button is selected, the window is closed and the home screen will be shown. Initialization failed will be displayed on top of all method screens. If the initialization test fails please contact the Implen Support Team.
  • Page 136 ® NanoPhotometer N120/NP80/N60/N50/C40 User Manual Version 4.3.1 The concentration of the sample used is too low for submicroliter cell measurements using Lid 5 with the Concentration too low. C40. Sample is below the detection limit of the instrument. Only cuvette measurement may be possible The concentration of the sample used is too low for Concentration too low.

  • Page 137: Important Alert Messages

    ® NanoPhotometer N120/NP80/N60/N50/C40 User Manual Version 4.3.1 a log file and send it to Implen customer support. The measurement parameters for 0.3 µl NanoVolume samples read only the 0.07 mm path length (dilution 140). The utilized sample Sample concentration too low for 0.3 µl - change concentration is too low.

  • Page 138: System Freeze

    Reboot the ® Server Not Available NanoPhotometer . If the message still appears please create a log file and send it to Implen Customer Support. Internal software communication problem during an open measurement session. Reboot the ®...

  • Page 139: Assistance

    Once the form is completed and the send button is selected a message will be sent directly to Implen and the appropriate support person will contact the end user as soon as possible to provide further support.

  • Page 140: User Manual

    AINTENANCE NPOS UPDATE Download the firmware update file (zip folder) from the Implen homepage: www.implen.de/downloads/ and unzip the file into the root folder of a USB flash drive. Note: Do not change the file names of the unzipped installation files.

  • Page 141: Create Log File

    USB flash drive. ® Recovery of the NanoPhotometer is only possible via the Implen support (remote access). ® In the case a NanoPhotometer recovery is necessary please get in touch with the Implen support team (support@implen.de).

  • Page 142: Diagnostics

    . Make sure that the measurement head is clean and no sample is applied and push the self-test button to start the self-test. As result reference and signal counts and the wavelength shift are shown. If the self-test is fails, please contact the Implen support (support@implen.de).

  • Page 143: License Information

    ® NanoPhotometer N120/NP80/N60/N50/C40 User Manual Version 4.3.1 ICENSE NFORMATION The NPOS software is Copyright of Implen and its affiliates. The NPOS software includes some open source software components under the licenses listed. (EULA) ICENSE GREEMENT Shows the end-user license agreement.

  • Page 144: Contact Implen

    User Manual Version 4.3.1 ONTACT MPLEN There is an option to contact Implen for instruments connected to the internet. For any support issues and questions please contact the Implen team directly: Europe, Asia, South Pacific, Middle East and Africa Implen GmbH Schatzbogen 52 81829 München...

  • Page 145: Maintenance

    Didymium glass filters and standard solution sets are suitable for the control and documentation of the wavelength accuracy and the photometric accuracy of your system. IQ/OQ documentation is also available. Please contact your local Implen office or an authorized Implen partner for further information.

  • Page 146: Cleaning And General Care

    ® NanoPhotometer N120/NP80/N60/N50/C40 User Manual Version 4.3.1  Touchscreen Replacement The optional touchscreen can only be assembled or replaced by the manufacturer or a certified service engineer from your supplier. LEANING AND ENERAL ® Switch off the NanoPhotometer and disconnect the power cord prior to external cleaning. Use a soft wet cloth or dry microfiber cloth to clean all external surfaces.

  • Page 147: Warranty

    The warranty is as defined in our current terms and conditions and is only valid if the product has been used according to the instructions supplied. Implen or your supplier can accept no liability of loss or damage arising from the faulty or incorrect use of this product...

  • Page 148: Appendix 1- Instalace Spektrofotometru

    ® NanoPhotometer N120/NP80/N60/N50/C40 User Manual Version 4.3.1 1- I PPENDIX NSTALACE PEKTROFOTOMETRU EZPEČNOSTNÍ INFORMACE Před zahájením instalace se prosím důkladně seznamte s výstražnými štítky a symboly na vašem přístroji a s jejich významem. Obsahují informace o tom, kde hrozí potenciální riziko a kde musíte být velmi obezřetní.

  • Page 149: Vybalení A Umístění

    NanoVolume. Zařízení musí být zapojeno do sítě pomocí napájecího zdroje / kabelu 90 W od společnosti Implen. Síťová zásuvka musí být vybavena ochranným vodičem (uzemněním). Lze používat se systémy napájecích zdrojů 90-250 V ± 10%, 50-60 Hz.

  • Page 150: Appendix 2 - Installatie Spectrofotometer

    ® NanoPhotometer N120/NP80/N60/N50/C40 User Manual Version 4.3.1 2 - I PPENDIX NSTALLATIE PECTROFOTOMETER EILIGHEIDSINFORMATIE Voordat u begint met de installatie, moet u de tijd nemen om de waarschuwingslabels en symbolen op het instrument te bekijken en te leren wat deze betekenen. Deze zijn aangebracht om u te informeren indien er een gevaar is of indien u specifieke voorzorgen moet nemen.

  • Page 151: Uitpakken En Plaatsen

    Draag het instrument steeds door het hoofdgedeelte van het instrument vast te nemen en niet door bijvoorbeeld het optionele scherm of de NanoVolume-steun vast te nemen. De apparatuur moet zijn aangesloten met het 90W-netsnoer/voedingskabel door Implen meegeleverd. Het stopcontact moet een aarding hebben. Het instrument is geschikt voor voedingen van 90-250 V ±...
  • Page 152 ® NanoPhotometer N120/NP80/N60/N50/C40 User Manual Version 4.3.1 Neem onmiddellijk contact op met de originele leverancier indien u technische problemen ervaart of problemen merkt met het verwerken van monsters Opmerking: Indien deze apparatuur wordt gebruikt op een andere manier dan hierboven vermeld of in een omgeving die niet geschikt is voor veilig gebruik, kan de voorziene bescherming van de apparatuur beschadigd raken en vervalt de garantie van het instrument.

  • Page 153: Ppendix Nstallation Du Spectrophotomètre

    ® NanoPhotometer N120/NP80/N60/N50/C40 User Manual Version 4.3.1 3 - I PPENDIX NSTALLATION DU SPECTROPHOTOMÈTRE NFORMATIONS DE SÉCURITÉ Avant de commencer l'installation, veuillez prendre le temps de vous familiariser avec les étiquettes et symboles de mise en garde de votre instrument, ainsi qu'avec leur signification. Ces étiquettes et symboles vous indiquent les situations dans lesquelles un risque potentiel existe, ou les cas où...

  • Page 154: Déballage Et Positionnement

    ® NanoPhotometer N120/NP80/N60/N50/C40 User Manual Version 4.3.1 Remarque: ne renversez aucun échantillon biologique sur les composants de l'instrument. Si cela se produit, désinfectez immédiatement l'instrument conformément aux protocoles de votre laboratoire et aux consignes de nettoyage de votre instrument (voir 8 Maintenance). Les échantillons biologiques peuvent contenir des maladies infectieuses ou avoir le potentiel de les transmettre.
  • Page 155 L'équipement doit être relié à l'alimentation secteur à l'aide du câble/bloc d'alimentation 90 W fourni par Implen. La prise électrique doit être équipée d'un conducteur protecteur (mise à la terre). L'équipement peut être utilisé avec un système d'alimentation 90–250 V ±...

  • Page 156: Appendix 4 - Installation Spectrophotometer

    ® NanoPhotometer N120/NP80/N60/N50/C40 User Manual Version 4.3.1 4 – I PPENDIX NSTALLATION PECTROPHOTOMETER ICHERHEITSINFORMATIONEN Vor Beginn der Installation nehmen Sie sich bitte die Zeit sich mit den Beschriftungen und Warnsymbolen auf Ihrem Instrument vertraut zu machen. Diese dienen als Information wo potenzielle Gefahren auftreten können bzw.

  • Page 157: Auspacken Und Platzieren

    Beim Transport des Gerätes nicht am optionalen Display oder Hebelarm tragen, sondern immer am Hauptgehäuse. Für den Betrieb des NanoPhotometers darf ausschließlich das 90W Netzteil / Kabel welches von Implen geliefert wurde verwendet werden. Stromversorgung: 90 - 250 V ±...
  • Page 158 ® NanoPhotometer N120/NP80/N60/N50/C40 User Manual Version 4.3.1 Schalten Sie das Gerät mit der Power-Taste auf der Rückseite des Gerätes ein, nachdem es eingesteckt ist. Das Instrument führt automatisch eine Reihe von Selbstdiagnoseprüfungen durch. Bitte lesen Sie die vollständige Bedienungsanleitung vor dem Gebrauch. Bitte kontaktieren Sie die ursprünglichen Lieferanten sofort, falls technische oder Handhabungs-Probleme auftreten.

  • Page 159: Appendix 5 - Installazione Dello Spettrofotometro

    ® NanoPhotometer N120/NP80/N60/N50/C40 User Manual Version 4.3.1 5 – I PPENDIX NSTALLAZIONE DELLO PETTROFOTOMETRO NFORMAZIONI DI SICUREZZA Prima di cominciare l’installazione, acquisire familiarità con i simboli e le etichette di avvertenza presenti sullo strumento e il loro significato. Simboli ed etichette segnalano potenziali pericoli oppure se occorre esercitare particolare cautela.
  • Page 160 NanoVolume. L’apparecchio deve essere collegato all’alimentazione utilizzando il cavo di alimentazione/l’alimentatore da 90 watt fornito da Implen. La presa di alimentazione deve essere provvista di conduttore di protezione (terra/massa). Può essere utilizzata con sistemi di alimentazione a 90-250 V ±...
  • Page 161 ® NanoPhotometer N120/NP80/N60/N50/C40 User Manual Version 4.3.1 Si prega di leggere il manuale utente completo prima del primo utilizzo. Dopo averlo collegato, accendere lo strumento utilizzando il pulsante di accensione sul pannello posteriore. Lo strumento esegue una serie di test di autodiagnostica. In caso di difficoltà...

  • Page 162: Informacje Dotyczące Bezpieczeństwa

    ® NanoPhotometer N120/NP80/N60/N50/C40 User Manual Version 4.3.1 6 – I PPENDIX NSTALACJA PEKTROFOTOMETRU NFORMACJE DOTYCZĄCE BEZPIECZEŃSTWA Przed rozpoczęciem instalacji należy zapoznać się z etykietami ostrzegawczymi i symbolami umieszczonymi na urządzeniu oraz z ich znaczeniem. Informują one o możliwym zagrożeniu lub o konieczności zachowania szczególnej uwagi. Niewłaściwe użytkowanie może doprowadzić...

  • Page 163: Rozpakowywanie I Umiejscowienie

    NanoVolume. Urządzenie należy połączyć ze źródłem zasilania przewodem zasilania o mocy 90 W dostarczonym przez firmę Implen. Gniazdo zasilania musi być uziemione. Urządzenie można stosować z systemami zasilania o napięciu 90–250 V ± 10%, 50/60 Hz.
  • Page 164 ® NanoPhotometer N120/NP80/N60/N50/C40 User Manual Version 4.3.1 używany. Ładowanie głęboko rozładowanych akumulatorów nie jest możliwe, ponieważ muszą one zostać wymienione na nowe. Przed pierwszym użyciem należy przeczytać pełną instrukcję obsługi. Po połączeniu urządzenia ze źródłem zasilania należy je włączyć przyciskiem zasilania na panelu tylnym.

  • Page 165: Información De Seguridad

    ® NanoPhotometer N120/NP80/N60/N50/C40 User Manual Version 4.3.1 7 – I PPENDIX NSTALACIÓN DEL SPECTROFOTÓMETRO NFORMACIÓN DE SEGURIDAD Antes de comenzar con la instalación, dedique un tiempo a familiarizarse con las etiquetas y símbolos de advertencia del instrumento y con su significado. Son para informarle cuando existan daños potenciales o se requiera una precaución particular.

  • Page 166: Desembalaje E Instalación

    ® NanoPhotometer N120/NP80/N60/N50/C40 User Manual Version 4.3.1 Las muestras biológicas pueden contener o tener el potencial de transmitir enfermedades infecciosas. Tenga en cuenta los riesgos para la salud que presentan dichas muestras y use el equipo de protección adecuado. Manipule dichas muestras con el mayor cuidado y de acuerdo con los requisitos reglamentarios y organizativos aplicables antes de trabajar con dichos materiales potencialmente infecciosos.
  • Page 167 ® NanoPhotometer N120/NP80/N60/N50/C40 User Manual Version 4.3.1 El equipo deberá conectarse a la corriente con la fuente de alimentación/cable de 90 W suministrada por. La toma de corriente deberá tener protección con toma de tierra. Puede usarse con fuentes de alimentación de 90-250 V ± 10%, 50-60 Hz. Para el caso de modelos portátiles, la batería deberá...

  • Page 168: Säkerhetsinformation

    ® NanoPhotometer N120/NP80/N60/N50/C40 User Manual Version 4.3.1 8 – S PPENDIX PEKTROFOTOMETERINSTALLATION ÄKERHETSINFORMATION Innan installationen påbörjas, var vänlig ägna tid åt att bekanta dig med ditt instruments varningsetiketter och symboler samt deras innebörd. De finns där för att informera dig om var det föreligger en risk eller särskild försiktighet krävs.

  • Page 169: Uppackning Och Placering

    NanoVolume-pelaren. Utrustningen måste vara ansluten till ström med 90 W strömförsörjning/sladd som levererats av Implen. Strömuttaget måste ha en skyddsledare (jord). Den kan användas med strömförsörjningssystem med 90-250 V ± 10%, 50-60 Hz. För mobila modeller, var vänlig ladda batteripaketet minst 3 timmar innan första användning.
  • Page 170 ® NanoPhotometer N120/NP80/N60/N50/C40 User Manual Version 4.3.1 Obs: Om den här utrustningen används på ett sätt som inte är föreskrivet eller i miljöförhållanden som inte är lämpliga för säker drift kan skyddet som utrustningen tillhandahåller försämras och instrumentets garanti förverkas.

  • Page 171: Appendix 9 - I

    ® NanoPhotometer N120/NP80/N60/N50/C40 User Manual Version 4.3.1 9 – I PPENDIX NSTALAÇÃO DE SPECTROFOTÓMETRO NFORMAÇÕES DE EGURANÇA Antes de começar a instalação, procure familiarizar-se com os símbolos e as etiquetas de aviso no instrumento e o respetivo significado. Destinam-se a informá-lo sobre onde se encontram os potenciais perigos ou onde é...
  • Page 172 O equipamento deve ser ligado à corrente através do cabo elétrico de 90W fornecido pela Implen. A tomada de corrente deve dispor de um condutor de proteção (ligação à terra). Pode ser usado num sistema de alimentação elétrica de 90-250 V ± 10%, 50-60 Hz.
  • Page 173 ® NanoPhotometer N120/NP80/N60/N50/C40 User Manual Version 4.3.1 Leia o manual do usuário completo antes da primeira utilização. Ligue o instrumento no botão de energia situado no painel traseiro, depois de ligado na tomada de corrente. O instrumento realiza um conjunto de verificações em auto-diagnóstico. Contacte imediatamente o fornecedor original se ocorrerem dificuldades técnicas ou no manuseamento de amostras.
  • Page 174 Icons ..............32 Solvent ............49, 55 IDS ............... 44 Concentration ............. 113 Illumination Sample Window ......125 Calculations ........... 116 Implen Document Source ........44 Measurement Protocol ........113 Initialization Test ..........134 Connecting Cable ..........16 Installation Connectivity Computer ............
  • Page 175 ® NanoPhotometer N120/NP80/N60/N50/C40 User Manual Version 4.3.1 Measurement Protocol Single Channel .... 58 Kinetics ..............93 Calculation ............95 Measurement Protocol ........93 OD600 ..............96 Calculations ............. 99 Measurement Protocol ........97 On/Off Switch ......10, 11, 12, 13, 14, 15 Lamp ..............
  • Page 176 ® NanoPhotometer N120/NP80/N60/N50/C40 User Manual Version 4.3.1 Remeasure ............92 Standard Solution ..........17 Rear Panel ............15 Stored Methods ..........47, 122 Replacement Parts ..........144 Stored Results ............ 121 Battery ............144 Submicroliter Cell ........17, 33, 52 Lamp ..............

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