Alternating Column Regeneration - Agilent Technologies G1157A User Manual

4 Valve Applications

Alternating Column Regeneration

Alternating column regeneration is a convenient way to increase the sample
throughput. The Agilent 1200 Series 2 position/ 10 port valve (G1157A) can be
used in combination with the Agilent LC and LC/MS modules to increase the
efficiency in laboratories running large amounts of samples.
Gradient elution is a common technique for separation of complex samples in
liquid chromatography, which requires column regeneration before the
subsequent run is started. Using alternating column regeneration valuable
time for the analysis is saved. Core of the alternating column regeneration is
the Agilent 1200 Series 2 position / 10 port valve, which allows simultaneous
analysis on one column while a second identical column is flushed and
equilibrated. The schematic setup is displayed in
Figure 18
40
Alternating Column Regeneration (schematic setup)
Alternating Column Regeneration
Figure 18.
Agilent 1200 Series Valves