Ratio Parameter; Time Vs Fluorescence Plot - Beckman Coulter Navios Instructions For Use Manual

Operation Principles
Parameters
Figure 3.10 Time vs Fluorescence plot
The minimum stop time is 10 seconds, the maximum stop time (maximum duration) is
1,200 seconds and the default stop time is 300 seconds (5 minutes).
When you assign the TIME parameter to a plot axis, the divisions on the axis change accordingly.
To find the time (in seconds) per channel in a one-parameter histogram, divide the stop time (in
seconds) by 1,024 (0.001 second = 1.0 ms).
For a two-parameter plot, divide by 64, 128, 256 or 512 depending upon the plot resolution you are
using.

RATIO Parameter

The RATIO parameter is calculated, not acquired directly. When you select a parameter, you specify
which signal is the numerator and which is the denominator.
RATIO =
----------------------------------- -
Denominator
A ratio of 1 is at channel 1,023. If you assign RATIO to a plot axis, RATIO events appear at a lower
channel if the intensity of the numerator signal is less than the denominator signal.
To calculate the actual ratio at a particular intensity for a one-parameter histogram, divide the
intensity by 1,024. For a two-parameter plot, divide by 64, 128, 256 or 512 depending upon the plot
resolution you are using.
3-12
Numerator
×
1024
773232AH