Integral Signal; Integral And Peak Pulses - Beckman Coulter Navios Instructions For Use Manual

Operation Principles
Signal Processing

Integral Signal

Because the total fluorescence in all three cells is the same, but the distribution is different, the
pulse can be integrated to produce an integral signal (see
The height of the integral pulse is proportional to the total fluorescence and is obtained when the
cell exits the laser beam. The pulse height, however, represents the most intense amount of
fluorescence produced. The area under the pulse is proportional to the total fluorescence.
Figure 3.8 Integral and Peak Pulses
1. Direction of sample flow
2. Cell in laser beam
3. Peak pulses
3-8
Figure
4. Volts
5. Time
6. Integral pulses
3.8).
7. Volts
8. Time
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