Beckman Coulter Navios Instructions For Use Manual page 322

Flow cytometer
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Using Navios Software
Analysis Menu
If the Region Properties dialog for any of the quadrants is opened via right click in Protocol explorer
or double clicking the name in the View / Modify regions dialog, then changing a QC product for a
quadrant is only applied to this quadrant.
QC Product
Select the QC product from the drop down list. See also,
NOTE
In a networked database configuration, when QC Product is added or changed by one user it is not
visible to another user in the networked configuration unless the user logs out and logs back in to refresh
the Region Properties dialog box QC Product list.
AutoGating
None
This is the default when the AutoGate option is not selected.
Elliptical
Toggle the AutoGate to an elliptical region.
Contour
Toggle the AutoGate region to a contour region.
Sensitivity
Eliptical AutoGate settings are 0.11, 0.33 and 1.1 (% of processed peak). Contour AutoGate settings
are 1, 2, 3, 4, and 5.
• Level 1 = 0.11% processed peak at a resolution of 64x64.
• Levels 2, 3 and 4 = 0.11, 0.33 and 1.1% at a resolution of 128x128.
• Level 5 = 1.1 % processed peak at a resolution of 256x256.
Selecting 0.11 creates a larger region, whereas selecting 1.1 creates a smaller radius region around
the target population.
Travel
Input a maximum value the contour AutoGate region travels from the mean. The Minimum value is
25; Maximum is 200 channels. Use this option to slightly adjust the AutoGate from tube to tube if
slight changes in lysis cause the target population to move slightly. This option is not available for
elliptical AutoGates.
10-126
Setting Up QC
Products.
773232AH

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