Agilent Technologies 1260 Infinity Quick Start Manual page 55

Characterizing the delay volumes: Mixing point to UV detector and Column to UV detector
Part
UV Cell
Total
Preparative System
The Mixing point to UV detector tubing volume of the preparative system or
combined analytical and preparative 1260 system is calculated between the
T-connection that connects the preparative pumps and the UV detector cell.
The calculation of the Mixing point to UV detector tubing volume comprises
two steps (modify the tables in these steps for a customized setup):
1 Calculate Mixing point to column delay volume
Table 8
Item
T-connection (preparative pumps)
T to mixer (0.6 × 40 mm)
Mixer (79835-87330)
Mixer to 2/10 valve (0.5 × 800 mm)
Valve to autosampler (0.5 × 600 mm)
Dual loop autosampler(G2258A), 5 mL loop
Autosampler to 2/10 valve (0.5 × 600 mm)
Valve to column (0.5 × 600 mm)
Total Preparative (mixing point to column)
Table 9
Item
Quat. Pump G1311B, Water/ACN gradient
Quat. pump to 2/10 valve (0.17 × 700)
Valve to autosampler (0.5 × 600 mm)
Purification Solution - Developer's Guide
Description
Max-Light Cartridge, 10 mm cell
Preparative flow path of combined instrument:
Analytical flow path of combined instrument:
1
Calibration Procedures
Volume, mL
0.001
0.258 + injected volume
Volume, mL
0.002
0.011
0.75
0.156
0.117
5.13
0.117
0.117
6.40
Volume, mL
p /2760 + 0.742
0
0.016
0.118
6
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