Agilent Technologies 1260 Infinity Quick Start Manual page 53

Characterizing the delay volumes: Mixing point to UV detector and Column to UV detector
Dead volumes of
The dead volume of some modules is dependent on pressure and solvent;
Agilent pumps
therefore, the table contains a calculation of the dead volumes for
Water/Acetonitrile and Water/Methanol gradients using the initial pump
pressure (p
Pump
G1311A/C
G7111A
1120 Compact LC
1220 VL Compact LC
G1311B
G7111B
1220 Compact LC
G5611A
G1312A/C
G1312B
G7112B
G4204A
G7104A/C
G4220A/B
G7120A
Dead volumes of
Because of the flow-through design, the injected volume must be added to the
Agilent
module's dead volume.
autosamplers
Purification Solution - Developer's Guide
• If used, heat exchange capillary in the thermostat of the column
compartment (TCC)
• Column to detector
•
Inlet capillary of the UV detector cell
•
Dead volume of the UV detector cell
, bar) at the beginning of the gradient.
0
Volume, mL
Water/ACN (p , bar)
0
p /2000 + 0.76
0
p /2760 + 0.742
0
p /2860 + 0.725
0
p /3330 + 0.76
0
No mixer: p /20000 + 0.205
0
Mixer: p /4000 + 0.77
0
No mixer: 0.46
V380: 0.85
No mixer: 0.033
V35: 0.075
V100: 0.16
V380: 0.39
Calibration Procedures
Volume, mL
Water/MeOH (p , bar)
0
p /4000 + 0.82
0
p /6060 + 0.787
0
p /2860 + 0.725
0
0.865
No mixer: 0.225
Mixer: p /6670 + 0.83
0
No mixer: 0.46
V380: 0.85
No mixer: 0.033
V35: 0.075
V100: 0.16
V380: 0.39
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