Reichert Univar User Manual page 27

Simultaneous darkfield fluorescence in transmitted-light
(possible only with mirror house 4)
⚪ Turn on halogen lamp and HBO 200W mercury vapor lamp
⚪ Turn on relay (red dot). For weak fluorescent objects, it can be
switched off
⚪ Turn off Bertrand lens (pull out pin)
⚪ Switch off neutral density filter in the body (black dot not
visible)
⚫ Set the blocking-filter slide for UV darkfield to the white
marked filter
⚫ Attach the UV blocking shade
⚪ Switch off the compensator
⚪ Pull out the interference contrast main prism to the stop
⚫ With the immersion darkfield condenser, use the 40x
objective. When working with the dry darkfield condenser, use
the 25x objective. If the condenser is on the quick-coupler, use
the 10x objective
⚫ Position the immersion darkfield condenser and oil with
immersion oil (Cargille D/A). Use the aperture insert In the dry
darkfield condenser for apo 25x and plan 40x objectives
⚪ Swing out the transmitted-light polarizer
⚪ Turn lower condenser turret to empty opening (red dot)
⚫ Set condenser fine drive in the middle of its travel and raise
the condenser with coarse feed up to the stop. Build a uniform
liquid contact between specimen slide and the immersion
condenser
⚪ Turn off the wide-field condenser auxiliary optics (red dot)
⚫ Place UV light blocking tube on light well
⚪ Switch off exciter or color filter for transmitted-light (red dot)
⚪ Switch off neutral density filters for transmitted-light (red dot)
⚪ Adjust the halogen lamp transmitted-light collector (red dot)
⚪ Set photo magnification changer (red dot)
⚪ Direct beam path to the camera (red dot, CAM)
⚫ Direct 20% beam to the body (red dot, CAM/PRO)
Use position EYE for weak fluorescent preparations
⚪ Set the relay phase ring knob to empty (red dot)
⚪ Set the relay magnification changer to 1x (red dot)
⚫ Set the rotary prism to transmitted-light with high-
performance lamp
⚫ Set the red filter or neutral density filters for simultaneous
fluorescence
⚫ Set the exciter filter U2 for UV darkfield
⚪ Set sliding mirror to halogen lamp (red dot)
⚫ Turn off the automatic zoom lighting (lever to the side)
⚫ Set manual zoom lighting to DF
⚫ Fully open condenser iris diaphragm
⚪ With coarse and fine condenser controls, focus illumination
from halogen lamp on the specimen
⚫ Close field iris aperture and focus on the aperture image with
condenser fine adjustment
⚫ Center field iris diaphragm with centering screws to the
center of the field of view, then open field iris diaphragm to just
larger than the field-of-view
⚫ Set the sliding mirror to high-performance light and adjust for
best fluorescence image with the high-performance lamp
collector
⚫ Set the sliding mirror for mixed light and adjust the brightness
of the image of the halogen lamp on the fluorescence image
B17