Reichert Univar User Manual page 16

B) with high-performance lamp
The adjustment of the microscope and the microscopic
image is done in the same way as described for the halogen
lamp
⚪ Switch to the high-performance lamp with corresponding
power supply
⚫ Set rotary prism for transmitted-light
⚫ When working with mirror house 4, set the white-coded
exciter filter. Working with mirror house 2, set exciter filter
to red dot
⚫ Set the sliding mirror for high-performance lamp
⚪ Engage the neutral density filters for transmitted-light as
needed.
⚫ Adjust the collector of the high-performance lamp so that
the field is just moderately bright
Phase ring knob with phase and anoptral annuli
The internal turret contains the phase and anoptral rings
(i.e., the diffraction or phase plates, which are built into the
objective lenses in ordinary microscopes) that the relay
system places into the optical path. For the 10x objective,
the phase ring with 10 and anoptral ring with 10A are used.
The rings of the other lenses are also marked with the
objective magnification. The position 0 of the knob, the red
dot position, provides an empty hole in the beam path.
Phase ring selection
The phase annuli (annular diaphragms) are in the lower
turret of the condenser. The correct centering of each phase
or anoptral annulus to the image of the corresponding ring
phase plate should be done after changing objectives. For
the ring aperture to be centered:
First as in A) described, set the illumination with 10x
objective. Set the lower condenser turret to 10Ph , and the
phase ring knob to 10. Carefully center the field iris
diaphragm.
The Bertrand lens pin is then pushed in so that the
superimposed images of the phase annulus and the
diffraction plate are visible.
By turning the knurled head of the Bertrand lens pin, the
lens is focused on the plate and the annulus. Using the two
➀
centering wrenches on the underside of the condenser,
press the wrenches to engage, and then turn them
individually to shift the images and make them concentric.
➁
The centering screws on the condenser carrier, which
serve to center the field iris diaphragm, must not be
adjusted during the above procedure.
B6