Reichert Univar User Manual page 25

Simultaneous contrast fluorescence in transmitted-
light (possible only with mirror house 4)
⚪ Turn on halogen lamp and 200W HBO mercury vapor lamp
⚪ Turn on relay (red dot)
⚪ Turn off Bertrand lens (pull out pin)
⚪ Turn off neutral density filters in the ocular body (black dot
not visible)
⚫ Slide into place the yellow blocking filter on the slide
⚫ Attach the UV blocking shade
⚪ Switch off the compensator
⚪ Pull out the interference contrast main prism until it stops
⚪ Move the 10x objective into position
⚫ Position the fluorescence immersion condenser or the
brightfield immersion condenser and oil with immersion oil
(Cargille D/A)
⚫ Set condenser fine drive in the central position and, with
the coarse feed, raise the condenser up to the stop. A
uniform liquid contact should be formed between the
specimen slide and the condenser
⚪ Swing out the transmitted-light filter polarizer
⚫ Turn to the 10Ph position of the lower condenser turret
⚫ Place UV blocking light tube on the light well
⚪ Turn off exciter filters for transmitted-light (red dot)
⚪ Turn off neutral density filters for transmitted-light (red
dot)
⚪ Switch off the auxiliary optics for wide-field condenser (red
dot)
⚪ Adjust the collector for transmitted-light halogen lamp
(red dot)
⚪ Set the photo magnification changer to red dot
⚪ Direct the beam path to the camera (red dot, CAM)
⚫ Direct 20% beam to the body (red dot, CAM/PRO)
For weak fluorescent objects, select position EYE
⚫ At the relay phase ring knob, select 10 for phase contrast,
or 10A for anoptral contrast
⚪ Set the relay magnification changer to 1x (red dot)
⚫ Set the rotary prism to transmitted-light with high-
performance lamp
⚫ Use red or neutral density filters for contrast fluorescence
⚫ Turn to exciter filter U3
⚫ Set the sliding mirror to halogen lamp (red dot)
⚪ Turn on automatic zoom lighting (red dot)
⚪ Fully open condenser iris diaphragm
⚪ Focus on the specimen with coarse and fine adjustment
using halogen illumination
⚫ Close field iris aperture and focus on the aperture image
with condenser fine adjustment
⚫ Center field iris diaphragm with centering screws to the
center of the field of view, then open field iris diaphragm to
just larger than the field-of-view
⚫ Set the sliding mirror to high-performance light and adjust
for best fluorescence image with the high-performance lamp
collector
⚫ Set the sliding mirror for mixed light and adjust the
brightness of the image of the halogen lamp on the
fluorescence image
⚪ When changing objectives, use the corresponding phase
ring or anoptral ring. The condenser iris can be closed to
control fluorescence when using different magnification
B15
objectives