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Reichert Univar User Manual

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  • Page 1 Reichert Univar Manual...
  • Page 3 Translated from the 11/1975 German language edition, with slight modifications. William R. Porter San Marcos CA USA 2017 v 1.4...
  • Page 4 In a very few places, the text has been rewritten slightly for clarity. Any mistakes are probably mine. No other Univar manuals or literature were used (nor indeed available) in the production of this one. This manual was translated purely for the use of myself; it is being made available to the public, free of charge, for their own use and enjoyment, with all the usual disclaimers and the understanding that they do so at their own risk, etc., etc.

  • Page 5: Table Of Contents

    Table of contents A) Setup and assembly....………………..............A1 B) Univar transmitted-light setup Transmitted-light brightfield microscopy……..............B1 Transmitted-light darkfield microscopy…………............. B3 Transmitted-light phase and anoptral contrast……............…..B5 Transmitted-light microscope with polarized light …............…B7 Transmitted-light interference contrast……………..……….………………….......B9 Transmitted-light brightfield fluorescence…………………………………....……….. B11 Transmitted-light darkfield fluorescence……………………………….……....…………… B13 Simultaneous contrast fluorescence with transmitted-light...………………………..…….
  • Page 6 (E) Trimatic camera system ……………………………………………………………………….... E1 Installation and connection of the Trimatic camera system ………………………………….. E3 Operation ………………………………………………………………………………...……….... E5 Special cases: Point measurement ..……………………………………………………………………..E7 Fluor button …..……..…………………...……………………………………………..E7 Double exposure .……………………………………………………………………....E7 Half-frame photograph ...…………….…………………………………………………..E7 Extension factors …………..…………………………………………………………….... E8 Interruption of exposure ..……………………………..…………………………………………..E8 Lock Gate ………………………………………………………………………………………....
  • Page 7 Three handles are screwed into the base plate, and are used to lift the microscope stand. The tripod weighs approx. 55 kg (121 lb) and should be lifted by 2 people. The desk on which the Univar is positioned must be stable, since the microscope with all of its associated gear can weigh up to 120 kg (264 lb).The countertop must be hard and flat, so...
  • Page 8 Assembly of the stage: The stand has a dovetail mount and the stage support has a mating dovetail. There are cutouts on each dovetail that allow the two to be fastened together. First, unscrew the large screw with a coin slot, ➂, near the top of the stage dovetail.
  • Page 9 Attaching the mirror housing: Remove the back of the mirror housing, after loosening the four corner fasteners by turning each fastener counterclockwise 1/4 turn. Fasten the housing to the microscope stand with the captive allen screw inside the housing (about a third of the way down in the center) using a long allen wrench or other hex key tool.
  • Page 10 Projection attachment: The projection tower is placed on the microscope stand, with the cylindrical light inlet over the opening of the stand. Carrying handles: The three carrying handles can be unscrewed from the base of the microscope stand. The threaded holes are provided with screw plugs.

  • Page 11: Transmitted-Light Brightfield Microscopy

    Transmitted-light brightfield microscopy A) with halogen lamp: When doing transmitted-light brightfield microscopy with the halogen lamp, the controls of the Univar are set at the red- dot positions. ⚪ Turn on halogen lamp with control transformer ⚫ Turn on relay (red dot) ⚫...
  • Page 12 B) with high-performance lamp: Adjustment of the microscope and the microscopic image is done in the same way described for the halogen lamp. ⚪ Turn on the desired high-performance lamp. ⚫ Turn the rotary prism for light from the HBO lamp ⚫...

  • Page 13: Transmitted-Light Darkfield Microscopy

    Transmitted-light darkfield microscopy A) with halogen lamp: ⚪ Switch to the halogen lamp with control transformer ⚪ Relay switch: red dot ⚪ Turn off the Bertrand lens (pull out pin) ⚪ Switch out the eyepiece neutral density filters (black dot not visible) ⚫...
  • Page 14 B) With high-performance lamp The adjustment of the microscope and the microscopic image is done in the same way as described for the halogen lamp ⚪ Switch to the high-performance lamp: switch on the desired high-performance lamp with corresponding power supply ⚫...

  • Page 15: Transmitted-Light Phase And Anoptral Contrast

    Transmitted-light phase- and anoptral-contrast A) with halogen lamp: ⚪ Turn on halogen lamp with control transformer. Turn on relay (red dot). ⚪ Set Bertrand lens off (pull out the pin) ⚪ Turn off neutral density filters in the body (no black dot) ⚪...
  • Page 16 B) with high-performance lamp The adjustment of the microscope and the microscopic image is done in the same way as described for the halogen lamp ⚪ Switch to the high-performance lamp with corresponding power supply ⚫ Set rotary prism for transmitted-light ⚫...

  • Page 17: Transmitted-Light Microscope With Polarized Light

    Transmitted-light microscopy with polarized light: (A) with halogen lamp ⚪ Turn on halogen lamp with control transformer ⚪ Turn on relay (red dot) ⚪ Turn off Bertrand lens (pull out pin) ⚪ Turn off neutral density filter in the ocular body (black dot not visible) ⚫...
  • Page 18 B) with high-performance lamp The adjustment of the microscope and the microscopic image is done in the same way as described for the halogen lamp ⚪ Switch on the desired high-performance lamp with corresponding power supply. ⚫ Redirect this light with rotating prism into the microscope for transmitted-light ⚫...

  • Page 19: Transmitted-Light Interference Contrast

    Transmitted-Light Interference Contrast (A) with halogen lamp ⚪ Turn on halogen lamp power supply ⚪ Turn on relay (red dot) ⚪ Disengage Bertrand lens (pull out pin) ⚪ Turn off neutral density filters in the ocular body (no black dot) ⚪...
  • Page 20 B) with high-performance lamp The adjustment of the microscope and the microscopic image is done in the same way as described for the halogen lamp. ⚪ Switch on the desired high-performance lamp with corresponding power supply. ⚫ Set the rotating prism for transmitted-light. ⚫...

  • Page 21: Transmitted-Light Brightfield Fluorescence

    Transmitted-light brightfield fluorescence: ⚪ Turn on HBO 200W mercury-vapor high-pressure lamp ⚪ Set light path to relay (red dot). With weak fluorescent objects it can switched off ⚪ Disengage Bertrand lens by pulling out pin ⚪ Disengage neutral density filters in the ocular body (black dot not visible) ⚫...
  • Page 22 Fluorescence immersion condenser N.A.: 1.35 oil Free working distance: 0.37mm Use: For fluorescence microscopy in brightfield, with objectives between 10x and 100x, to meet the highest requirements of brightness and contrast. Also for working with normal light with 4x objective (use the frosted 4 filter on the lower condenser turret) Brightfield immersion condenser N.A.: 1.30 oil...
  • Page 23 Transmitted-light darkfield fluorescence: ⚪ Turn on HBO 200 W mercury vapor high-pressure lamp ⚪ Turn on relay (red dot). For weak fluorescent objects, it can be switched off ⚪ Pull out pin of Bertrand lens (set off) ⚪ Turn off neutral density filters in the ocular body (black dot not visible) ⚫...
  • Page 24 Immersion darkfield condenser N.A.: 1.18 -1.42 oil Free working distance: 0.38mm Slide thickness: 1.1 ± 0.1mm Use: for all objectives between 40x and 100x. A cardioid condenser, fully suitable for fluorescence studies. Dry darkfield condenser N.A.: 0.7-0.9 Free working distance: 4.8mm Slide thickness: 1.1 ±...
  • Page 25 Simultaneous contrast fluorescence in transmitted- light (possible only with mirror house 4) ⚪ Turn on halogen lamp and 200W HBO mercury vapor lamp ⚪ Turn on relay (red dot) ⚪ Turn off Bertrand lens (pull out pin) ⚪ Turn off neutral density filters in the ocular body (black dot not visible) ⚫...
  • Page 26 Fluorescence immersion condenser N.A.: 1.35 oil Free working distance: 0.37mm Use: For fluorescence microscopy in brightfield, with objectives between 10x and 100x for highest requirements of brightness and image contrast. When working with normal light, usable with 4x objective; the lower condenser turret must be turned to 4, the frosted auxiliary lens.
  • Page 27 Simultaneous darkfield fluorescence in transmitted-light (possible only with mirror house 4) ⚪ Turn on halogen lamp and HBO 200W mercury vapor lamp ⚪ Turn on relay (red dot). For weak fluorescent objects, it can be switched off ⚪ Turn off Bertrand lens (pull out pin) ⚪...
  • Page 28 Immersion darkfield condenser N.A: 1.18 -1.42 oil Free working distance: 0.38mm; slide thickness: 1.1 ± 0.1mm Use: for all objectives between 40x and 100x A cardioid condenser, fully suited for fluorescence studies Dry darkfield condenser N.A.: 0.7-0.9 Free working distance 4.8mm; slide thickness: 1.1 ± 0.1mm Use: In combination with the lower condenser turret, can work with lenses 25x and 40x.
  • Page 29 Immunofluorescence with epifluorescence attachment With the epifluorescence optics carrier, the interference splitter mirror set on 40 can perform all transmitted-light and simultaneous methods. To achieve best specimen detail we recommend transmitted-light phase contrast (page C9) or transmitted-light darkfield (page C7). Immunofluorescence: ⚪...
  • Page 30 Epi-illumination swivel filter holder: The swivel filter holder is screwed into the side of the microscope stand. It contains the following filters which can be combined with the filters of the epifluorescence illuminators: Markings on the swivel arms: S (white).…..Empty special filter (2-11mm thick) I (black)...…….
  • Page 31 Conventional fluorescence and quinacrine with epifluorescence With the epifluorescence illuminator, you can simultaneously perform all illumination methods using the interference beamsplitter mirror 40. For isolation of paler details we recommend transmitted- light phase contrast (page C9) or transmitted-light darkfield (page C7) to sharpen the specimen image Conventional fluorescence and quinacrine: ⚪...
  • Page 32 Field iris diaphragm & aperture slot for microphotometry There is an iris diaphragm in the epifluorescence illuminator light pipe. This is adjusted to prevent UV fading of large areas of the specimen. Opening the field iris diaphragm is done by rotating the knurled ring, while focusing is done by loosening the hex lock pin and using it to slide the cylindrical part towards or away from the observer, then retightening the pin.
  • Page 33 Detection of aromatic amines with epifluorescence With the epifluorescence illuminator, you can simultaneously perform all light methods using the interference beamsplitter mirror 40. For isolation of paler details, we recommend transmitted-light phase contrast (page C9) or transmitted-light darkfield (page C7) to sharpen the specimen image. Verification of aromatic amines ⚪...
  • Page 34 Field iris diaphragm aperture illumination for microphotometry There is an iris diaphragm in the epifluorescence illuminator light pipe. This is adjusted to prevent UV fading of large areas of the specimen. Opening the field iris diaphragm is done by rotating the knurled ring, while focusing on the iris is done by loosening the hex lock pin and using it to slide the cylindrical part towards or away from the observer, then...
  • Page 35 Mixed illumination: epifluorescence + transmitted- light darkfield, epifluorescence + transmitted-light darkfield fluorescence A) transmitted-light darkfield with halogen lamp: ⚪ Turn on halogen lamp with control transformer ⚪ Turn on relay (red dot) ⚪ Turn off neutral density filters in the ocular body (dot not visible) ⚪...

  • Page 36: Transmitted-Light Darkfield Fluorescence

    B) epifluorescence ⚪ Turn on the HBO 200W mercury-vapor high- pressure lamp ⚪ Turn off the halogen lamp ⚪ Attach the UV-blocking eyeshade ⚫ Switch the interference beam-splitting mirror between 40 and 56, according to the desired excitation, for immunofluorescence (page C1), aromatic amines (page C5), or conventional fluorescence (page C3) ⚫...

  • Page 37: Mixed Illumination: Epifluorescence + Transmitted-Light Phase And Anoptral Contrast

    Mixed illumination: epifluorescence + transmitted-light phase- and anoptral-contrast (A) Transmitted-light phase- and anoptral-contrast, halogen lamp ⚪ Turn on halogen lamp. Turn on relay (red dot) ⚪ Turn off Bertrand lens (pull out pin) ⚪ Turn off neutral density filters in ocular body (no black dot) ⚪...
  • Page 38 B) epifluorescence ⚪ Turn on HBO 200W mercury-vapor lamp ⚪ Turn off the transmitted-light halogen lamp ⚪ Mount the UV blocking shade ⚫ Switch to the desired exciter interference beam- splitting mirror (40 to 56) - as described (page C1), aromatic amines (page C5), or conventional fluorescence (page C3) for immunofluorescence ⚫...
  • Page 39 Mixed illumination: epifluorescence + transmitted-light interference contrast A) Transmitted-light interference contrast with halogen lamp ⚪ Turn on halogen lamp ⚪ Turn on relay optics (red dot) ⚪ Turn off Bertrand lens (pull out pin) ⚪ Turn off neutral density filters in the ocular body (black dot not visible) ⚪...
  • Page 40 B) epifluorescence ⚪ Turn on HBO 200W mercury-vapor lamp ⚪ Turn off the transmitted-light halogen lamp ⚪ Mount the UV blocking shade ⚫ According to the desired exciter, move slide to interference beam-splitting mirror 40 to 56 for immunofluorescence (page C1), aromatic amines (page C5) or conventional fluorescence (page C3) ⚫...
  • Page 41 Mixed illumination: epifluorescence +transmitted- light polarization A) Polarization with halogen lamp ⚪ Turn on halogen lamp with control transformer ⚪ Turn on optical relay (red dot) ⚪ Disengage Bertrand lens (pull out pin) ⚪ Rotate off the neutral density filters in the ocular body (black dot not visible) ⚪...
  • Page 42 B) Epifluorescence ⚪ Turn on the HBO 200W mercury vapor lamp ⚪ Turn off the halogen lamp ⚪ Mount the UV blocking shade ⚫ For the desired UV exciter, set the interference beam-splitting mirror from 40 to 56 for immunofluorescence (page C1), aromatic amines (page C5), or conventional fluorescence (page C3) ⚫...

  • Page 43: Mirror House 4

    Mirror House 4 Swivel mirror for Epi-illumination collector for Transmitted light halogen lamp Lamp house 50 Epi-illumination halogen lamp Rotary Prism: trans/epi swivel ground glass for DL Collector for right-hand light Collector for left high- Transmitted light filter performance light hub U Lamp house 25L Filter selector CF for...
  • Page 44 Filter hub N for transmitted-light ⬤ (Red dot)……..……………………… Empty opening ⬤ ….………….………………………………. Neutral density filters 32x2 N3, T=12.5% ⬤ ….………….……………………………… Neutral density filters 32x2 N6, T=1.6% ⬤ ….………….………………………………. Neutral density filters 32x2 N9, T=0.2% ⬤ ….………….……………………………… Neutral density filters 32x2 N12, T=0.025% ⨂...

  • Page 45: Mirror House 2

    Mirror House 2 Rotary Prism: transmitted-light / Clear/Diffuse for epi-illumination transmitted light Collector for right- Collector hand light adjustment Lamp house 10 or Lamp 25 or 50 25 L Filter hub S for Filter hub N for transmitted-light transmitted-light On the mirror house 2, two bulbs of your choice can be attached, e.g., the halogen lamp on the left, and the lamp house 25 or 50 on the right.
  • Page 46 Filter hub N for transmitted-light ⬤ (Red dot)……..…..………………………..... IR-blocking filter 32x1.7 KG 1/h ⬤ ….………….…………………………….…... Neutral density filters 32x2 N3, T=12.5% ⬤ ….………….…………………………….…... Neutral density filters 32x2 N6, T=1.6% ⬤ ….………….…………………………….…... Neutral density filters 32x2 N9, T=0.2% ⬤ ….………….…………………………….…... Neutral density filters 32x2 N12, T=0.025% ⨂...

  • Page 47: Lamp House 10

    Lamp house 10 The lamp house 10 houses the 100W low-voltage halogen bulb. Insert the 100W low voltage halogen light bulb ➂ ➀ ⚫ Loosen the cam screw , pull out the lamp socket ➃ from the housing. Hold the new bulb with optical tissue, and insert its pins into the lamp pin holes while pressing together the spring-loaded clamp levers.
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  • Page 49: Lamp House 25

    Lamp House 25 The lamp house 25 is manufactured in two versions; one mounted on the left side of the mirror house, the other on the right side. The illustration shows the left-mounted version. The following lamp can be used in the lamp house 25: HBO 200W/4 or CS 200W/4 mercury vapor high-pressure lamp;...
  • Page 50 ⚫ Turn to the 10x objective, and insert the 10x eyepieces into the observation tube. ⚫ Ignite the lamp, and with no specimen on the stage, set the Univar to transmitted-light brightfield microscopy with epi-illumination ⚫ Switch off the ground glass ⚫...
  • Page 51 Lamp house 50 The lamp house 50 can hold either the XBO 450W Xenon lamp or the HBO 200 W/2 mercury-vapor high-pressure lamp Installation of the XBO 450W/2 Xenon lamp: PLEASE NOTE! Before opening the lamp house, unplug the connection cable. The lamp contains a pressure of several atmospheres.
  • Page 52 ⚫ Turn to the 10x objective, and insert the 10x eyepieces into the observation tube. ⚫ Ignite the lamp and with no specimen on the stage, set the Univar to transmitted-light brightfield with the high-performance lamp for the light source ⚫ Swivel ground-glass off.
  • Page 53 Power supply connections: Up to three lamps may be supplied from the power supply shown, with connectors for the following lamps: ⚫ 100W low-voltage halogen lamp ⚫ HBO 200W/4 or CS 200W/4 mercury-vapor high- pressure lamp ⚫ CSI 250W/1 metal halide short-arc lamps ⚫...

  • Page 54: Power Supply For Csi 250W/1 Metal-Halide Short-Arc Lamp

    Connections for HBO 200W/4 or CS 200W/4 mercury-vapor high-pressure lamp 1. safety starter 2. hour counter 3. power switch 4. connector for lamp 25 The power supply is intended for 220VAC. If the line voltage is not 220V, the intermediate transformer, page D13, is connected upstream.

  • Page 55: Intermediate Transformer

    Intermediate transformer 1. main switch 2. power connection 3. voltage selector ⚫ The intermediate transformer is used if the supply voltage differs from 220V. It is installed upstream of the other power supplies. ➁ ⚫ The line connection is via connector ⚫...

  • Page 56: Power Supply For Xbo 450W Xenon Lamp

    Power supply for 450W/2 xenon lamp 1. test button for voltage 2. voltmeter 3. current controller 4. ammeter 5. main switch 6. fuse, 8A 7. pilot light 8. fuse, 1A 9. connection for control cable for the remote control unit or jumper 10.

  • Page 57: (E) Trimatic Camera System

    E. Trimatic Camera System Camera with Polaroid Camera with 4”x5” Automatic camera, 35mm film cassette, 3 1/4” International film 24 x 36mm format x 4 1/4” back...
  • Page 58 (blank, no content)

  • Page 59: Installation And Connection Of The Trimatic Camera System

    Installation and connection of the Trimatic camera system You can place three different cameras in any arrangement on the Univar. ⚫ To install, turn cover plate counterclockwise and remove. ⚫ Line up the locking bayonet pins on the male camera tube with the slots in the cavity, insert the camera and turn clockwise to lock into place.
  • Page 60 ⚫ The connection cable of the automatic camera with the multiple plug is connected to the associated connector at the rear of the microscope. There are three female connectors: one for a camera on the left, the second for a camera on the right, and the third for a top-mounted automatic camera.

  • Page 61: Operation

    Operation ⚫ Turn on controller box with main switch. The switch cap lights up red, along with the exposure time display, as well as more signals that indicate a state of readiness. ⚫ Select camera The data for all three cameras are stored in the controller simultaneously.
  • Page 62 ⚫ Adjust the photo system magnification changer to L, normal magnification. In special cases, increasing magnification by a factor of 1.6x is possible by switching to ⚫ For all cameras, the same image section, which is located in the formatting eyepiece within the limit lines, is photographed.

  • Page 63: Special Cases: Point Measurement

    Special cases Point measurement Normally, the light measurement is integrated, i.e., an average value of all brightnesses occurring in the object is measured. When the button is switched on, it is possible to select individual object details, which are within the double circle, if the brightness of specimen zones varies a lot.

  • Page 64: Extension Factors

    (a) the optical path is not directed to the camera: the two levers on the right side of the Univar are not in the position CAM or CAM/PRO (red dot positions) (b) the lever of the magnification changer for the...

  • Page 65: Magnification In The Film Plane

    Magnification in the film plane Photomicrograph image scale (M) is calculated according to the formula: M = Vobj * q * Vok * P Vobj........Magnification of the lens q........Relay magnification Vok......Enlargement by the Photo eyepiece P........Camera factor The table below provides information on the product Vok * p, considering the camera format and the positions L or H of the magnification changer in the photosystem...

  • Page 66: Camera With Lnternational Camera Back, Format 4" X 5

    Camera with international camera back, format 4”x 5” Accommodates traditional double film-holders for plates and film, 9x12 cm or 4x5, and the Polaroid cut-film holder Model 545, by means of an international spring camera back. In use, the frame of the spring-loaded frame is lifted slightly, and the film holder is inserted between the camera housing and the frame.
  • Page 67 Automatic camera for 35mm film (24 x 36mm) To remove the cassette (which looks like a 35mm camera body) from the camera, the locking lever must be moved forward, and the red light "error" illuminated on the control unit, so that the automatic operation is blocked. At the same time, the cassette shutter closes and the cover lock is fixed so that the lid can be removed and the cassette can be removed.
  • Page 68 ⚫ After loading the cassette with film, make sure the transport key is raised before pushing the cassette into the camera housing. Be sure that the transport key is parallel to the film plane. This will ensure a proper engagement with the driving cam. The lid is then placed horizontally and pressed with one hand as far as it will go, while the other hand reverses the locking lever.

  • Page 69: Coarse Drive Stop

    The special accessories were already described in the different working methods. In the following, you will find general instructions that make it easier for you to work with the Univar. Ocular body ➂ The Interpupillary distance is set with the knurled control knob , and can be ➀...
  • Page 70 HF: Brightfield DLF: Transmitted fluorescence excitation DFI: Darkfield with immersion darkfield condenser AFBL: Epi-illumination fluorescence with blue light excitation DFT: Darkfield with dry darkfield condenser AFUV: Epi-illumination fluorescence w/ UV excitation PHC: Phase and phase anoptral contrast Interference contrast The engravings on the lens barrel have the following meanings: Plan ....

  • Page 71: Relay System

    Relay system The relay system is an apochromatic transport optical path that can be inserted between objectives and eyepiece. It generates intermediate pictures of the image field and the objective pupil. In this path are then made the necessary interventions in the optical path of the microscope, while lenses and eyepieces remain unchanged.

  • Page 72: Projection Attachment

    Projection attachment The projection tower has a diameter of 240mm, this corresponding to a field of view number of 24. Projection magnification = Objective magnification x 10x the magnification in the relay ⚫ Lever position PRO ⚫ Lever position CAM/PRO (red dot) ⚫...

  • Page 73: Care Of The Microscope

    This endeavor requires occasional changes to the mechanical and optical construction of improvable instruments. All descriptions and illustrations in catalogs and instructions for use, as well as all numerical information on mechanical structure and optical data, are therefore not binding. 21.25/1 GA - UNIVAR - D - 11-75...

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