Troubleshooting - GE SimpliNano Original Instructions Manual

3. TROUBLESHOOTING

Detailed in the table below are the most common faults found whilst using our
spectrophotometers
Instrument
Ultrospec 10
Ultrospec 10
All instruments
All instruments
All instruments
All instruments
All cuvette models
Novaspec III
Novaspec III
Novaspec III
Novaspec plus
Novaspec plus
Novaspec plus
GeneQuant 100/1300,
Ultrospec 2100,
Ultrospec 7000/8000/9000
GeneQuant 100/1300,
Ultrospec 2100,
Ultrospec 7000/8000/9000
29-0047-78 AC 04-2014
Problem
A flashing Absorbance reading of 2.00 Abs is
obtained.
REF is displayed when T is pressed.
A negative reading is obtained.
Unexpected results are obtained.
Absorbance values higher than expected.
Absorbance values lower than expected.
Instrument will not zero.
Error code 001 symbol
Error code 006 symbol
Error code Flashing number!
X against Lamp on initialisation screen.
X against Wavelength calibration on
initialisation screen.
- against all calibration items on
initialisation screen.
Poor reproducibility on DNA concentration
and purity measurements.
Instrument fails start up calibration check.
Possible causes
This indicates an Absorbance of more than
1.99 and is therefore out of range. The
sample needs to be diluted.
The instrument has not been zeroed
before a sample measurement has been
attempted.
In normal measurements the test sample
has a positive Absorbance compared to that
of the Reference. Negative readings will be
obtained if the Reference and Test cuvettes
are mixed up.
Any bubbles in solution will produce
considerable error.
Possible issue with optical alignment.
Contact service support.
Check sample cuvette filled to 20 mm from
base of cuvette.
Possible stray light issue. Contact service
support.
Cuvette placed in wrong orientation, Wrong
cuvette material used for wavelength.
Lamp needs changing.
.
Pixel clock too high. Instrument needs
.
optical alignment. Contact service support.
Wavelength accuracy failure. Instrument
needs optical alignment. Contact service
support.
Lamp needs changing.
Check sample beam is clear when
instrument switched on.
Instrument needs optical realignment or
reprogramming. Contact service support.
Wrong cuvette being used – not a 15 mm
beam height or wrong material – not letting
UV light pass through.
Background correction mode not switched
on.
Measured Absorbance too low, should be
>0.1 A for accurate results.
Check sample and reference beams are
clear when instrument switched on.
GeneQuant 100/1300, Ultrospec
2100/7000/8000/9000 report failure
message to service support.
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