Important Guidelines; Operation - VWR International 250V Instruction Manual

Important Guidelines

Important guidelines for operating the 300V Power Supply are provided in this section. We
recommend that you carefully review these guidelines before operating the instrument.
For best results, do NOT use the 300V Power Supply at its maximum
Important:
electrical load limits. Variations in buffer conditions can result in exceeding the power supply's
maximum voltage, current, or power output capacity and produce undesirable variations in
electrophoretic separations.
General Operating Instructions
Follow the instructions below to operate the 300V Power Supply.
Turn on the 300V Power Supply by pressing the power switch on the rear side of the
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instrument. Upon start-up, the Display Screen on the front of the instrument will illuminate.
Use the START/PAUSE and STOP keys to switch on and off the power to the output jacks.
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Recommendation
The duration of electrophoresis can be defined in time (hours/minutes). When using this or any
electrophoresis product, we recommend that you adhere to the time protocols provided in
protocol and application manuals.
For best results, follow these important guidelines when running multiple
Important:
gels and electrophoresis units concurrently.
Avoid running samples with differing buffer salt concentrations at the same time.
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Note: Variations in conductivity due to differences in buffer salt concentrations can affect the run of all the
samples run at the same time.

Operation

The 300V Power Supply is designed to operate under two modes, Constant Voltage Mode or
Constant Current Mode, depending upon your electrophoresis application.
Use the Constant Voltage / Current Mode for applications that require only one specific voltage
limit or current limit during the entire duration of electrophoresis.
Display Screen
The Display Screen illuminates after turning on the power to your instrument and the factory
default settings (or last settings used) will be displayed. You can choose the operational
Mode (Constant Voltage or Constant Current Mode) by pressing the "constant" button.
6/22/2012
Revision 1.0
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