Epi-Fl Microscopy; Epi-Fl Microscopy - Nikon Eclipse LV100DA-U Instructions Manual

Universal design microscope udn
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6

Epi-fl Microscopy

Attach the accessories required for the epi-fl microscopy.
1
The following accessories must be attached to perform the epi-fl microscopy.
• Filter cube for the epi-fl microscopy (attached to the LV-UEPI2A) (See Page 84.)
• External light source (Intensilight C-HGFIE or EXFO X-Cite 120 PC, used when the brightness
of the halogen lamp is insufficient.) (See Page 89.)
* If only the simplified polarization microscopy is performed, the PA block (LV-PAB) can be used instead of the
analyzer and the polarizer.
Find the target and focus on it by bright-field or dark-field microscopy under the epi-
2
illumination.
(See Pages 15 to 17.)
Set the microscope for the epi-fl microscopy.
3
1 Press the CUBE switch on the operation panel
and light up the "FL1" or "FL2" position of the
microscopy method indicator. (See Page 37.)
The light source is adjusted to the predetermined
light quantity, and the aperture diaphragm for
the episcopic illumination is adjusted to the
predetermined size automatically by the
interlock control function.
2 Open or close the shutter of the light source with
the EPI switch on the operation panel, and adjust
the brightness with the EPI brightness switch.
(See Pages 41 and 42.)
3 Adjust the brightness with ND filters.
(See Page 44.)
About the shutter of the light source
To prevent fading of the specimen, make sure to close the shutter when you don't observe the
specimen. The shutter of the light source can be opened and closed with the EPI switch on the
operation panel.
24
F. ST OP
1
BF
DF FL1 FL2
FL1
FL2
0.8
0.7
Achr N.A
0.6
= 0.9
0.5 0.4
JAPAN
0.3 0.2 0.1
Operation
panel
ND8
F.S.
2
EPI switch
1
CUBE switch
A.S.
OBJ.
CUBE
3
UEP I2A
USB
RS2 32C
LCN T
NCB
2
EPI brightness
switch
EPI
EPI
DIA
DIA

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